A Holiday Gift Buying Guide for your GC: Latest …...A Holiday Gift Buying Guide for your GC:...
Transcript of A Holiday Gift Buying Guide for your GC: Latest …...A Holiday Gift Buying Guide for your GC:...
A Holiday Gift Buying Guide for your GC: Latest Supply Innovations
Alexander UcciOnline Application EngineerNovember 12, 2019
November 12, 20191 Latest GC Supply Innovations Agilent Restricted
Receive a 25% discount off any Agilent J&W GC columns, Agilent LC columns, sample preparation products, chemical standards, and your most often used GC and LC supplies.*
• Discount applies on future purchase of up to $3,000 (USD)
• Offer valid for up to 30 days after the Agilent webinar
• Use promotion code 9969
*Some restrictions apply on supplies
November 12, 20192
Exclusive Offer for Attending Agilent Chemistries Webinars
Latest GC Supply Innovations Agilent Restricted
An Overview of the Latest Supply Innovations
Gas traps
Crimpers
SeptaLinersGold sealsColumn nuts
A-line vials
Latest columnsFID jets
Sample cleanup
ADM FlowmeterStandards
November 12, 20193 Latest GC Supply Innovations Agilent Restricted
Let’s Talk About Gas Quality and Filters
• Oxygen in carrier gas is detrimental to GC/MS– Reduced response– Elevated background– Irreversible column damage– Impaired electron multiplier function– Premature filament, liner lifetime
• Use UHP carrier gases– 99.9995% or greater
• Use Gas Clean carrier gas filters
4
~5% O2
Decreased retention
Red
uced
resp
onse
GC/MS filterAgilent p/nCP17973
November 12, 2019 Latest GC Supply Innovations Agilent Restricted
Let’s Talk About Gas Quality and FiltersIf you used lower quality gases, how much O2 could the filter clean up?
5
He
Installing (and properly purging) the Gas Clean carrier gas filter lowered the O2 signal by a factor of 5.
Extracted Ion Chromatogram (EIC) for O2
November 12, 2019 Latest GC Supply Innovations Agilent Restricted
Let’s Talk About Gas Quality and FiltersIf you used lower quality gases, how much H2O could the filter clean up?
Gas Clean filter lowered the H2O signal by a factor >10.You can further increase Gas Clean and column lifetime by installing the universal filter before the Gas Clean filter.
6
HeExtracted Ion Chromatogram (EIC) for H2O
November 12, 2019 Latest GC Supply Innovations Agilent Restricted
Let’s Talk About Gas Quality and FiltersIf lower quality gases were used, how much background could the filter clean up?
• Install the Universal trap vertically – use the mounting bracket(s)• Extend the lifetime of your Gas Clean (indicating) filter and your column
7
HeBig Universal trap
GC/MS filterAgilent p/nCP17973
RMSH-2
Mounting bracketUMC-5-2
November 12, 2019 Latest GC Supply Innovations Agilent Restricted
New Agilent A-Line Electron Crimpers and Decappers
• More vials crimped per battery charge• Increased crimping speed when compared to previous
models• Lighter weight means less hand strain and effort• OLED screen for easier viewing interface• More efficient charging• New motor life is significantly longer leading to extended
productivity
November 12, 20198
https://www.agilent.com/en/products/lab-supplies/chromatography-spectroscopy/vials-closures/crimpers-decappers-accessories
Latest GC Supply Innovations Agilent Restricted
• Choose high-quality vials and caps– Poorly constructed vial septa siloxanes bleed peaks
– Low-quality vial leach contaminants into sample
– Choose the right cap/septa for your solvent
9
Vials
November 12, 2019 Latest GC Supply Innovations Agilent Restricted
ADM Flow Meter: Easy No-Return Recalibration
10
• Replaceable calibration cartridge
• Automatic notification of cartridge replacement
• Ergonomic and robust design
• Universal 3AA or USB power
• USB connects to Web interface for added functionality
• Easy to view OLED Screen
• Kickstand
Technical overview 5991-7685EN
November 12, 2019 Latest GC Supply Innovations Agilent Restricted
If your target ions are buried beneath matrix peaks, it might be time to trim the column or do sample cleanup
The Matrix
500 ppb ethenoproxin black tea
10 ppb linalool in shampoo
(or improve your sample cleanup)
November 12, 201911 Latest GC Supply Innovations Agilent Restricted
How Did it Become Contaminated in the First Place?
12 November 12, 2019 Latest GC Supply Innovations Agilent Restricted
November 12, 2019
Bond Elut Solid Phase Extraction cartridges and plates
Captiva EMR-Lipid filtration cartridges and plates
Captiva syringe filters
Offline Options for Sample Matrix Removal
QuEChERS
Filter vials
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Synthetic Chem Elut S
Latest GC Supply Innovations Agilent Restricted
• Environmental analyses of water samples• Odor analyses (ppt)• Flavor analyses of food products• Forensic analyses of arson/explosives samples• Toxicology analyses: blood alcohol or drugs in
urine/serum• Surfactants, other industrial applications
• Trace analysis in food• Drugs and pharmaceuticals• Herbicides/pesticides• Medical diagnostics• Trace impurities in polymers and solid samples• Solvent residues in raw materials• Explosives
Solid Phase Microextraction (SPME)
14
SPME fibers SPME arrows
SPME Fiber and Arrow Offering from Agilent
November 12, 2019 Latest GC Supply Innovations Agilent Restricted
Polycyclic aromatic hydrocarbons (PAHs) are a large class of organic compounds containing two or more fused aromatic rings. PAHs are considered compounds of concern by every environmental organization; their concentration in water is strictly regulated.
15
5994-1301EN
Examination of Lower Molecular Weight PAHs in Drinking Water Using Agilent PDMS SPME Fibers
SIM chromatogram of naphthalenes with PDMS fibers (black trace = 100 µm; green trace = 30 µm; blue trace = 7 µm)
SIM chromatogram of fluoranthene and pyrene with PDMS fibers (black trace = 100 µm; green trace = 30 µm; blue trace = 7 µm)
6x10
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3.7+EI SIM(128.0) SIM CTC_100PDMS_PAH_25uL_SIM_REP007.D
8.786
Counts vs. Acquisition Time (min)
8.7 8.75 8.8 8.85 8.9 8.95 9 9.05 9.1 9.15 9.2 9.25 9.3 9.35 9.4 9.45 9.5 9.55 9.6 9.65 9.7 9.75 9.8 9.85
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+EI SIM(202.0) SIM CTC_30PDMS_PAH_25uL_SIM_REP007.D
15.088
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Counts vs. Acquisition Time (min)
15 15.05 15.1 15.15 15.2 15.25 15.3 15.35 15.4 15.45 15.5 15.55 15.6 15.65 15.7 15.75
Naphthalene1-Methylnaphthalene
2-Methylnaphthalene FluoranthenePyrene
November 12, 2019 Latest GC Supply Innovations Agilent Restricted
Liquid/Liquid Extraction (LLE)
• LLE has been successfully used as a method of sample preparation for many years.
• It separates the more organic solvent soluble compounds from the more water-soluble compounds using water immiscible organic solvents.
• It can remove many interfering substances like salts• Modulating pH can selectively extract or eliminate specific
compound types.
16 November 12, 2019 Latest GC Supply Innovations Agilent Restricted
Drawbacks of Liquid/Liquid Extraction
• LLE does have drawbacks– Inconsistent results from one analyst to another
• Shaking time• Shaking motion• Determination of where to cut between layers
– Emulsions– Labor intensive– Quite tedious with small sample sizes (<5 mL)– Challenging with large numbers of samples– Difficult to automate for large numbers of samples
How many of these problems can be fixed with Solid Supported Liquid Extraction?
Latest GC Supply Innovations Agilent RestrictedNovember 12, 201917
Load
Add solvent
Gravity elute
Add aqueous sample
Hold 5 min
1. Add aqueous sample to SLE sorbent2. Wait 5 minutes3. Elute with water immiscible solvent
How Does SLE Work?
18 November 12, 2019 Latest GC Supply Innovations Agilent Restricted
• There are two types of SLE media– Diatomaceous earth (DE) based products like our Chem Elut brand of SLE products
• A mined fossil diatom material, which is heterogeneous and inconsistent from one mine to the next
– Synthetic media we use in Chem Elut S• Controlled synthesis to be consistent batch after batch
What is SLE Sorbent?
Diatomaceous Earth
in Chem Elut
Synthetic SLE sorbent
× Naturally occurring; mined
× Broad particle size distribution
× Supplier reliability issues
× Poor lot-to-lot consistency
Large scale synthesis
Narrow particle size distribution
Reliable supplier Controlled manufacturing
19 November 12, 2019 Latest GC Supply Innovations Agilent Restricted
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Recovery (30 µg/mL; n = 3), 87-119% Chem Elut S 5 mL Chem Elut S 10 mL Chem Elut S 20 mL
0.01.02.03.04.05.06.07.08.09.0
10.0Reproducibility (30 µg/mL; n = 3), RSD 0.4-8.2%
Chem Elut S – 15 Minute Hold TimeLarge scale format comparison with aromatic amines
20
5994-0951EN
Latest GC Supply Innovations Agilent RestrictedNovember 12, 2019
Enhanced Matrix Removal: Agilent Captiva EMR-Lipid
Free fatty acids
Phospholipids
Triglycerides
EMR-Lipid sorbent technology effectively traps lipids through two mechanisms:• Size exclusion – unbranched hydrocarbon chains (lipids)
enter the sorbent; bulky analytes do not
• Sorbent chemistry – lipid chains that enter the sorbent are trapped by hydrophobic interactions
Analyte
Lipid
EMR sorbent
November 12, 2019 Latest GC Supply Innovations Agilent Restricted21
Determination of 19 Polycyclic Aromatic Hydrocarbon Compounds in Salmon and Beef
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Using Captiva EMR-Lipid cleanup by GC/MS/MS
5994-0553EN
Latest GC Supply Innovations Agilent RestrictedNovember 12, 2019
Captiva EMR-Lipid Cleanup Improves Analytes S/N Ratio and Integration Accuracy on GC/MS(/MS)
Pesticide
+ MRM (151.0 -> 79.1) AV MBPI-a-CD C2 spike 50ppb-1.D
Acquisition Time (m
12.7 12.8 12.9 13 13.1 13.2 13.3 13.4 13.5 13.6
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13.163 min.
+ MRM (151.0 -> 79.1) AV Z-Sep+ C5 spike 50ppb-1.D
Acquisition Time (m
12.7 12.8 12.9 13 13.1 13.2 13.3 13.4 13.5 13.6
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ts 5x10
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13.163 min.
+ MRM (183.1 -> 168.1) AV MBPI-a-CD C2 spike 50ppb-1.D
Acquisition Time (m
20.4 20.5 20.6 20.7 20.8 20.9 21 21.1 21.2
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ts 4x10
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520.788 min.
+ MRM (183.1 -> 168.1) AV Fatty dSPE spike 50ppb-1R.D
Acquisition Time (m
20.4 20.5 20.6 20.7 20.8 20.9 21 21.1 21.2
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20.833 min.
+ MRM (183.1 -> 168.1) AV Z-Sep+ C5 spike 50ppb-1.D
Acquisition Time (m
20.4 20.5 20.6 20.7 20.8 20.9 21 21.1 21.2
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20.819 min.
Captan Permethrin
EMR-Lipidcleanup
Zirconia sorbentcleanup
C18/PSAcleanup
Deltamethrin
+ MRM (181.0 -> 152.1) AV a-CD pow C3 spike 50ppb-1 .D
Acquisition Time (m
22.2 22.3 22.4 22.5 22.6 22.7 22.8 22.9 23
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ts 4x10
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1.722.628 min.
Ratio = 169.4 (488.3 %)
+ MRM (181.0 -> 152.1) AV Z-Sep+ C5 spike 50ppb-1.D
Acquisition Time (m
22.2 22.3 22.4 22.5 22.6 22.7 22.8 22.9 23
Coun
ts 4x10
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Ratio = 116.9 (337.0 %)
+ MRM (181.0 -> 152.1) AV Fatty dSPE spike 50ppb-1R.D
Acquisition Time (m
22.2 22.3 22.4 22.5 22.6 22.7 22.8 22.9 23
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ts 4x10
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22.645 min.
Ratio = 103.9 (299.5 %)
5994-0405EN
Latest GC Supply Innovations Agilent RestrictedNovember 12, 201923
ULTRA Scientific is Now Part of Agilent Technologies
24
Agilent ULTRA Chemical Standards have:• Best in class online search, compare, and
ordering capabilities• Rapid shipping: 99.9% of orders dispatched within
24 to 48 hours• Custom solutions• Sample preparation materials, columns, supplies,
instrumentation, and reference materials from a single source
• Rigorously tested and manufactured under ISO 9001, ISO 17025, and Guide 34 certifications
You can calibrate with confidence and maximize accuracy
November 12, 2019 Latest GC Supply Innovations Agilent Restricted
An Overview of the Latest Supply Innovations
Gas traps
Crimpers
SeptaLinersGold sealsColumn nuts
A-line vials
Latest columnsFID jets
Sample cleanup
ADM FlowmeterStandards
25 November 12, 2019 Latest GC Supply Innovations Agilent Restricted
InletSepta
Liner
Gold seal
Column ferrule and nut
• Injection efficiency:– Main function of the inlet is to produce a narrow
sample band at the head of the column– One of the most important aspects to any high-
resolution GC method• Must be reproducible• The liner volume must be large enough to
accommodate the solvent’s phase transformation into a vapor (backflash)
• Most chromatography problems are “front-end” related
• Many consumables to replace: septa, liner, gold seal
• Inlet body must be cleaned/solvent rinsed periodically (no steel brushes, please)
Latest GC Supply Innovations Agilent RestrictedNovember 12, 201926
30+ Years of R&D Focused on Surface Deactivation
UltiMetal Plus
• Inorganic vapor deposition in Middelburg
• CFT device
• Ultimate union
• Flexible metal ferrule
• Inlet welding
• Detector parts
• GC tubing
Ultra Inert
• Organic vapor deposition– Ultra Inert inlet liners– Ultra Inert gold seals
• Unique proprietary deactivation process
– UI columns
27
Two unique chemistries were developed to treat the surfaces in a GC flow path.
November 12, 2019 Latest GC Supply Innovations Agilent Restricted
Agilent Inert Flow Solution
Agilent UltiMetal Plus– TCD, FPD, NPD/FID jets
Agilent UltiMetal Plus capillary flow technologydevices, Ultimate union
Agilent J&W Ultra Inert GC column
Agilent Ultra Inert gold seal
Agilent Ultra Inert inlet liner
Agilent UltiMetal Plus ferrules
Agilent UltiMetal Plus inlet weldment, shell and transfer lines
5990-8532EN brochure
Latest GC Supply Innovations Agilent RestrictedNovember 12, 201928
Septa• Typical cost of one premium septum (list), $1.25
• Typical cost of one GC column, 30 m x 0.25 mm id, $450
• Proactively change inlet septa
• Agilent packing eliminates contamination of septa
• “CenterGuide septa” puts less train on syringe compared to solid septa
• Do not overtighten septum nut; septum can begin to “bulge” out
• Should tighten nut until c-clamp on top stops turning, then ½ to ¾ turn more
Removed during PM
29 November 12, 2019 Latest GC Supply Innovations Agilent Restricted
Septum nut
Ultra Inert Inlet Liners
1. Ultra Inert deactivated inlet liners provide higher response for sensitive compounds
2. Ultra Inert glass wool liners deliver benefits of glass wool without loss of active compounds
3. QC tested and certified for consistent performance
ProductivityTouchless packaging with pre-installed O-ring: quick and easy hassle free installation
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ndrin
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akdo
wn
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Restek Sky Liners (n=4) UI Liners (n=4)Competitor liners UI liners
Latest GC Supply Innovations Agilent RestrictedNovember 12, 201930
Agilent Assurance
UI liner lot QC with demanding test probes (dinitrophenol and others)Assurance of consistent performanceLabel for p/n, batch, and lot testingTraceabilityDeactivation lot number is on certificate
• Liner lot number (and part number) etched on glass
November 12, 2019 Latest GC Supply Innovations Agilent Restricted31
Packaging, Ease, and Productivity
Individual liner
25/pk in Touchless dispenserHigh throughput
Convenient 5/pk in Touchless packaging
100/pk, bulk, blister(no O-ring)High throughput
32 Latest GC Supply Innovations Agilent RestrictedNovember 12, 2019
What’s New? Glass Wool Alternative LinersUltra Inert liners with sintered glass frits
Low frit single taper
liner
• Designed for dedicated splitless analyses
• Ideal for SVOCs and OC/OP pesticides
Mid frit single
taper liner
• General purpose split/splitless liner
• Ideal for basic drugs analysis
Porous glass frit
33 November 12, 2019 Latest GC Supply Innovations Agilent Restricted
Modern Day Solution: The Glass-Frit
34
Substituting glass wool for a sintered glass frit provides equivalent vaporization properties while enhancing performance consistency and quality
November 12, 2019 Latest GC Supply Innovations Agilent Restricted
PesticidesOrganochlorine pesticidesEndrin and DDT
Acids
Semivolatiles
PhenolsAcid-base pairsBenzidine
Dislodged glass wool fibers expose active sites that interact with sensitive analytesThe Benefits of the Glass Frit
Interaction with active sites results in
Tailing
Degradation
Carryover
Response loss
35 Latest GC Supply Innovations Agilent RestrictedNovember 12, 2019
Low frit liner gives excellent response and peak shape for tricky analytes like phenols8270 Chromatogram Overlay – Phenols/Low Frit Liner
min9 9.2 9.4 9.6 9.8 10 10.2 10.4 10.6 10.8
pA
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Blue = Initial injection Red = After 100 matrix injectionsGreen = After liner replacement
Acenaphthene-d10
2,4-dinitrophenol
4-nitrophenol4,6-dinitro-2-methylphenol
5 ppm, 1 µL splitless injection FID detector
36 November 12, 2019 Latest GC Supply Innovations Agilent Restricted
min6 8 10 12 14
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FPD1 A, Front Signal (Shear\FPD_2293_10-50 2018-05-15 20-02-44\50ppb_2293_v2_F2.D)
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FPD1 A, Front Signal (Shear\FPD_P1_10-50 2018-05-18 08-54-29\50ppb_P1_v1_F2.D)
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7
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96
FPD1 A, Front Signal (Shear\FPD_P2_10-50 2018-05-22 10-19-20\50ppb_P2_v1_F2.D)
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9
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5 5.
555 6.
160
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3
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8 8.
446
8.81
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8
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92
FPD1 A, Front Signal (Shear\FPD_P3_10-50 2018-05-23 09-18-08\50ppb_P3_v1_F2.D)
5.17
3
5.40
6 5.
555
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0
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6.75
4
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3
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9 8.
447
8.81
5
9.80
9
11.76
4
12.45
6
13.01
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8
13.63
2
14.8
93
FPD1 A, Front Signal (Shear\FPD_P4_10-50 2018-05-17 10-35-43\50ppb_P4_v1_F2.D)
5.17
9
5.40
8 5.
556
6.16
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8.81
9
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7
FPD1 A, Front Signal (Shear\FPD_P5_10-50 2018-05-21 09-51-39\50ppb_P5_v1_F2.D)
5.19
4
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5 5.
554
6.15
9
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4
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4
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4
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3
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92
Results for fritted liner indicate highly inert, consistent responsePesticides by FPD
min8.2 8.3 8.4 8.5 8.6 8.7 8.8 8.9
150 pA
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FPD1 A, Front Signal (Shear\FPD_2293_10-50 2018-05-15 20-02-44\50ppb_2293_v2_F2.D)
8.39
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0
8.82
0
FPD1 A, Front Signal (Shear\FPD_P1_10-50 2018-05-18 08-54-29\50ppb_P1_v1_F2.D)
8.39
0
8.44
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8.81
7
FPD1 A, Front Signal (Shear\FPD_P2_10-50 2018-05-22 10-19-20\50ppb_P2_v1_F2.D)
8.38
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FPD1 A, Front Signal (Shear\FPD_P3_10-50 2018-05-23 09-18-08\50ppb_P3_v1_F2.D)
8.38
9
8.44
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8.81
5
FPD1 A, Front Signal (Shear\FPD_P4_10-50 2018-05-17 10-35-43\50ppb_P4_v1_F2.D)
8.39
1
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9
FPD1 A, Front Signal (Shear\FPD_P5_10-50 2018-05-21 09-51-39\50ppb_P5_v1_F2.D)
8.38
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4
Chlorpyrifos methyl
Fenitrothion
37 Latest GC Supply Innovations Agilent RestrictedNovember 12, 2019
Agilent UI Gold Seal: Deactivated Gold Surface
• Soft gold plating is essential for proper sealing • Ultra Inert chemistry blocks active sites (gold is notinert)• Smooth surface doesn't leak (injection molded)• Part numbers 5190-6144 ea. 5190-6145, 10/pk. 5190-6149, 50/pk.
Agilent MIM seal Competitor’s machined seal
Reliable ppb and ppt measurements require attention to the little things
November 12, 201938 Latest GC Supply Innovations Agilent Restricted
For mass spectrometry transfer lineNovember 12, 2019
39
• Spring driven piston continuously presses against ferrule
• Automatically retightens when ferrule shrinks
• No leaks, no downtime, no frustration
• Wing design for finger tightening
• No tools needed
• No polymer materials for durability
• Compatible with only short graphite • Vespel ferrules
For inlet or detector
Column Installation: Self-Tightening Column Nut
November 12, 2019 Latest GC Supply Innovations Agilent Restricted
40
Increasing Ease of Use Through Continued Innovation: Self-Tightening Nuts
For mass spectrometry transfer line
For GC inlet or detector
Innovating for the next generation
Innovating for the next generation
• Easier and faster to install
• Collar holds column in place
• Single-hand installation into inlet
• No tools needed
November 12, 2019 Latest GC Supply Innovations Agilent Restricted
• Spring-driven piston continuously presses against ferrule
• Automatically retightens when ferrule shrinks
• Wing design for finger tightening
• No tools needed
November 12, 201941
Self-Tightening Nuts: No leaks, No Downtime, No Frustration
400 injections
Part Number DescriptionG3440-81013 Column Nut, Collared Self-Tightening MSDG3440-81011 Column nut, Collared Self Tightening Inlet/DetectG3440-81012 Collar for Self Tigthening Nut
Latest GC Supply Innovations Agilent Restricted
An Overview of the Latest Supply Innovations
Gas traps
Crimpers
SeptaLinersGold sealsColumn nuts
A-line vials
Latest columnsFID jets
Sample cleanup
ADM FlowmeterStandards
Latest GC Supply Innovations Agilent RestrictedNovember 12, 201942
Low Polarity Mid Polarity High PolarityCP-Sil 2 DB & HP-1MS UI DB & HP-5MS UI DB-XLB DB-225MS DB-ALC1 HP-88 DB-WAX DB-WAX UI
DB-MTBE DB & HP1-MS DB & HP5-MS VF-XMS DB-225 DB-Dioxin CP-Sil 88 DB-WAX ETR DB-HeavyWAX
CP-Select CB MTBE VF-1MS VF-5MS DB-35MS UI CP-Sil 43 CB DB-200 DB-23 HP-INNOWax DB-FATWAX UI
DB & HP-1 DB & HP-5 DB & VF-35MS VF-1701 MS VF-200MS VF-23 MS VF-WAX MS
CP-Sil 5 CB CP-Sil 8 CB DB & HP-35 DB-1701 DB-210 CP-WAX 57 CB
Ultra 1 Ultra 2 DB & VF-17MS CP-Sil 19 CB DX-4 DB & HP-FFAP
DB-1HT VF-DA DB-17 HP-Blood Alcohol DB-WAX FF
DB-2887 DB-5.625 HP-50+ DB-ALC2 CP-FFAP CB
DB-Petro/PONA DB & VF-5HT DB-17HT DX-1 CP-WAX 58 FFAP CB
CP-Sil PONA CB CP-Sil PAH CB DB-608 CP-WAX 52 CB
DB-HT SimDis Select Biodiesel DB-TPH CP-WAX 51
CP-SimDis SE-54 DB-502.2 CP-Carbowax 400
CP-Volamine HP-VOC Carbowax 20M
Select Mineral Oil DB-VRX HP-20M
HP-101 DB-624 CAM
SE-30 VF-624MS CP-TCEP
CP-Select 624 CB
DB-1301
VF-1301MS
CP-Sil 13 CB
Agilent J&W columns have over 50 different stationary phase
offerings
Agilent J&W Column Portfolio- DB, HP, CP, VF
Latest GC Supply Innovations Agilent RestrictedNovember 12, 201943
Introducing DB-HeavyWAX
• WAX column with increased MAOT compared to existing columns on the market– 280 °C isothermal and 290 °C programmed
• Provides increased thermal stability• Has a low bleed level
General GC
• Shorter runtimes when late eluters are present
• Better S/N ratio, improved detection
• Better thermal stability• Faster column bake-out
GC/MS
• Desire for “zero” bleed• Avoid MS contamination by
column bleed for longer system uptime and column lifetime
• Improve detection limit
GC×GC
• Extended scope of compounds
November 12, 201944 Latest GC Supply Innovations Agilent Restricted
Advantages
Response Units (%) vs. Acquisition Time (min)1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 310
20
40
60
80
10
30
50
70
90
pA
DB-HeavyWAX at 100 hours
DB-HeavyWAX at 0 hours
WAX A at 0 hours
WAX B at 0 hours
WAX A at 100 hours
WAX B at 100 hours
Bleed Summary at 280 °C Over 100 Hours
Latest GC Supply Innovations Agilent RestrictedNovember 12, 201945
Thermal Stability and Retention Time Shifting for a Traditional WAX Phase
Peak Compound1 Methanol2 Benzene3 Toluene4 Ethylbenzene5 p-Xylene6 m-Xylene7 o-Xylene
Application note 5991-9035EN
2.4 2.5 2.6 2.7 2.8 2.9 3 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 3.9 4 4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 5 5.1 5.2 5.3 5.4 5.5 5.6 5.7 5.8 5.9 6 6.1 6.2 6.3 6.4 6.5 6.6 6.7 6.8 6.9 7 7.1 7.2 7.3 7.4 7.5 7.6 7.7 7.8 7.9 8 8.1 8.2 8.3 8.4
Initial BTEX injection
25 h at 280 °C
50 h at 280 °C
1
2
3
4 5 6
7
1 min earlier
Latest GC Supply Innovations Agilent RestrictedNovember 12, 201946
2.8 2.9 3 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 3.9 4 4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 5 5.1 5.2 5.3 5.4 5.5 5.6 5.7 5.8 5.9 6 6.1 6.2 6.3 6.4 6.5 6.6 6.7 6.8 6.9 7 7.1 7.2 7.3 7.4 7.5 7.6 7.7 7.8 7.9 8 8.1 8.2 8.3 8.4 8.5 8.6 8.7 8.8
Initial Injection
25 h at 280 °C
50 h at 280 °C
75 h at 280 °C
100 h at 280 °C1
2
3
4 5 67
Application note 5991-9035ENDB-HeavyWAXPeak Compound
1 Methanol2 Benzene3 Toluene4 Ethylbenzene5 p-Xylene6 m-Xylene7 o-Xylene
Only a few seconds of
shift
Latest GC Supply Innovations Agilent RestrictedNovember 12, 201947
Higher Final Temperatures Mean Earlier Elution of Heavy Compounds, Sharper Late Eluting Peaks, and Reduced CarryoverPink grapefruit essential oil (cold pressed)
2x10
-0.1-0.05
00.050.1
0.150.2
0.250.3
0.350.4
0.450.5
0.550.6
0.650.7
0.750.8
0.850.9
0.951
1.05 Final temp 250 °C1
2x10
-0.1-0.05
00.050.1
0.150.2
0.250.3
0.350.4
0.450.5
0.550.6
0.650.7
0.750.8
0.850.9
0.951
1.05 Final temp 280 °C1
Counts (%) vs. Acquisition Time (min)3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69
Initial Injection
Repeat Injection
Meranzin
Isomeranzin
Osthole
Carryover compounds
Application note 5991-9078ENLatest GC Supply Innovations Agilent RestrictedNovember 12, 201948
Better Peak Shape and Decreased Runtime
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
1.1
1.2
1.3
1.4
1.5
DB-HeavyWAXRun time: 13 minFinal temp: 280 °C Ramp: 30 °C/min Flow: 5 mL/min
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
1.1
1.2
1.3
1.4
1.5 DB-WaxRun time: 19 minFinal temp: 250 °C Ramp: 30 °C/min Flow: 5 mL/min
Response Units (%) vs. Acquisition Time (min)0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5 6 6.5 7 7.5 8 8.5 9 9.5 10 10.5 11 11.5 12 12.5 13 13.5 14 14.5 15 15.5 16 16.5 17 17.5 18 18.5 19 19.5 20
C9
C10
C18C16C14
C12
C20 C22 C24
C26
C28
C30C32
C34C36
C9
C10
C18C16C14
C12C20 C22
C24 C26 C28
C30
C32
C34C36
Final temp reached
Final temp reached
Latest GC Supply Innovations Agilent RestrictedNovember 12, 201949
2x10
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
1.1
1.2Agilent J&W DB-HeavyWAX
2x10
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
1.1
1.2
Competitor A
Counts (%) vs. Acquisition Time (min)27.5 28 28.5 29 29.5 30 30.5 31 31.5 32 32.5 33 33.5 34 34.5 35 35.5 36 36.5 37 37.5 38 38.5 39 39.5 40 40.5 41 41.5 42 42.5 43 43.5 44 44.5 45 45.5 46 46.5 47 47.5 48 48.5 49 49.5 50 50.5 51 51.5 52 52.5 53
Bleed 8.8 pA
Bleed 3.1 pA12
3
4 5 76
12
3
45
76
Bleed Reductions for FAMEs in Biodiesel (IP-585) Using a DB-HeavyWAX, 60 m x 0.25 mm x 0.50 µm
Peak FAME1 C16:02 C17:0-d33 (IS)3 C17:04 C18:05 C18:16 C18:27 C18:3
November 12, 201950 Latest GC Supply Innovations Agilent Restricted
• Great need in the food, forensic, and cosmetic industries to monitor the content of free and natural occurring fatty acids.
• Analysis of underivatized organic acids and free acids is desirable to eliminate the problems associated with derivatization, extraction, and cleanup procedures
• Volatile organic acids and fatty acids are difficult to quantify accurately by standard WAX columns. These acids often elute as tailing or poorly resolved peaks. For some acids, adsorption can become irreversible.
• Normally:– Fatty acids are derivatized to the methyl ester (FAME)– Free fatty acids are analyzed using acid-deactivated WAX columns. The acid modifier,
nitroterephthalic acid, however, reduces thermal stability, operating temperature and reacts with humidity, reducing column life.
DB-FATWAX UI Provides the Desired Inertness and Thermal Stability to Separate Challenging Organic Acids and Fatty Acids
Latest GC Supply Innovations Agilent RestrictedNovember 12, 201951
min5 10 15 20 25
pA
10
20
30
40
50
60
min5 10 15 20 25
pA
10
20
30
40
50
60
Compound Identification1. 2-Nonanone2 Decanal3. Propionic Acid4. Ethylene Glycol5. Heptadecane6. Aniline
7. Methyl Dodecanoate8. 2-Chlorophenol9. 1-Undecanol10. Nonadecane11. 2-Ethylhexanoic Acid12. Ethyl Maltol
1
1
2
2
3
3
4
4
5
5
6
6
7
7
8
8
9
9
10
10
11
12
1211
Standard acid-modified WAX
DB-FATWAX Ultra Inert
Comparison Between Acid-Modified WAX and DB-FATWAX UI After 50 h at 250 ºCDB-FATWAX Ultra Inert shows superior inertness and thermal stability compared to acid-modified WAX
52 November 12, 2019 Latest GC Supply Innovations Agilent Restricted
November 12, 2019
DB-FastFAME20 m x 0.18 mm x 0.20 μm
min1 2 3 4 5 6 7
C21:
0C2
0:3n
6C2
0:4n
6C2
0:3n
3
C20:
5n3
(EPA
)C2
2:0
C22:
1
C24:
0
C22:
2C2
3:0
C24:
1C2
2:6
(DHA
)C4:0
C6:0
C8:0 C1
0:0
C11:
0
C12:
0
C13:
0
C14:
0C1
4:1
C15:
0C1
5:1
C16
:0
C17:
0
C17:
1
C16:
1 C18:
0 C18:
1tra
ns
C18:
2 tra
ns
C18:
1 ci
s
C18:
2 ci
s C1
8:3
n 6
C18:
3 n
3
C20:
0C2
0:1n
9
C20:
2
C18
:1tr
ans
C18
:2 tr
ans
C18
:2 c
is
C18:1 cis
C20
:5n3
(EPA
)C
22:0
C24
:0
C24
:1C
22:6
(DHA
)
C20
:4n6
C20
:3n3
C21
:0C
20:3
n6
RS ≥ 1.95 for cis/trans isomers
RS = 1.51RS = 1.56
C21:0, C20:3n6, C20:4n6RS ≥ 1.52
Column Agilent J&W DB-FastFAME, 20 m x 0.18 mm, 0.20 µm Gas Hydrogen, 28 psi, constant pressure mode Inlet Split/splitless, 250 °C, split ratio 50:1 Oven 80 °C (0.5 min), 65 °C/min to 175 °C, 10 °C/min to 185 °C (0.5 min),
7 °C/min to 230 °C
FID 280 °C, hydrogen: 40 mL/min; air: 400 mL/min; make-up gas: 25 mL/min.
Injection 1 µL
TransCis
Strong interaction between cis isomers and the dipoles of the cyano propyl ligands. That allows the trans to elute after the cis isomers.
7 min analysis time
53 Latest GC Supply Innovations Agilent Restricted
54
Introducing the Agilent 8890 GC SystemFlexible and expandable to meet your needs today and tomorrow
Future-proof: Ready for anything — Powerful next generation electronic
architecture— Expanded smart-connected functionality— Full suite of inlets, detectors, and accessories,
CFT, Deans switch, backflush, GC x GC, dual simultaneous injection
— Six valves, eight heated zones, plus LVO— Generation 6 precision EPC— Smart keys— 7-inch color touch display
November 12, 2019 Latest GC Supply Innovations Agilent Restricted
55
Agilent 8890 GC SystemSmart-connected GC
Modern intuitive interface— 7-inch color touch screen
— Configuration— Status— Methods— Sequence info— Troubleshooting, diagnostics, and help
— Real-time chromatographic evaluation— Blank evaluation— Detector evaluation
November 12, 2019 Latest GC Supply Innovations Agilent Restricted
• Available with the Agilent 8890 GC only
• Can track use of a GC column
• Smart key contains GC column information, including:– Part and serial numbers– Number of injections/runs– Time at/above temperature limits– Date installed– Temperature limits – GC columns
• If more than one column is installed, the temperature is determined by lowest column smart key installed (DB-WAX vs DB-5)
– Column length/trimming done in “column maintenance mode” in software and rewritten to the smart key
– S/N of last instrument installed if it was in an 8890 GC
56
For immediate identification and usage monitoring of your GC columnGC Columns with Smart Key (for the Agilent 8890 GC only)
Latest GC Supply Innovations Agilent RestrictedNovember 12, 2019
57
Feature Advantage Benefit Economic BenefitSmart key included with the GC column p/n must include “KEY” Designation example*122-5532UI-KEY*
1. Reduce possible errors of manual input of method parameters
2. Optimized maintenance schedules with usage tracking
1. Better data quality as the system is aware of the configurationEase-of use
2. Ability to better plan preventive maintenance before issues occur
1. Ease-of-use, no risk of faulty information in GC, fewer reruns
2. Limited downtime as preventive maintenance is easier to plan
GC Columns with Smart Key
Feature, advantages, and benefits
Literature Web page
1 Agilent 8890 GC brochure https://www.agilent.com/cs/library/brochures/brochure-gc-8890-5994-0476en-agilent.pdf
2 Smart key product page (not for ordering smart keys)
www.Agilent.com/chem/smartkey8890
3 Instruction sheet https://www.agilent.com/cs/library/instructionsheet/public/insert-smart%20key-8890-5994-0700en-agilent.pdf
November 12, 2019 Latest GC Supply Innovations Agilent Restricted
• It is not a USB flash drive. It comes already programmed from the factory with specific information about the GC column that it belongs to
• It is not tethered to the GC column, neither is connected through it with WIFI or Bluetooth.
58
Smart key clarificationsGC Columns with Smart Key
Latest GC Supply Innovations Agilent RestrictedNovember 12, 2019
November 12, 201959
Expanding the Intuvo Column FamilyKey features• VF-, CP-, and Select- applications specific: 24 new phases and 39 new part numbers
• New Intuvo column dimension: 0.15 mm id to add to our 0.18 mm id for a greater range
of high-efficiency columns
• We now have PLOT columns
• Custom column options
VF-1ms VF-5ms CP-WAX 52 CB
CP-Volamine VF-17ms HP-PLOT Q
CP-Sil 5 CB CP-Sil 8 CB VF-WAXms Select Mineral Oil
VF-1701ms HP-PLOT Al2O3 S
CP-Sil 8 CB for Amines
CP-WAX 57 CB
Select PAH VF-Xms
CP-WAX 58 FFAP
Select Low Sulfur
VF-624ms
CP-Wax for Volamine
Select-FAME CP-Select 624 CB
CP-Sil 88 for FAME
CP-Sil 88
CP-TAP VF-200ms
PDMS 5% Phenyl WAX SpecialtySiloxane/ Cyanopropyl PLOT
Latest GC Supply Innovations Agilent Restricted
An Overview of the Latest Supply Innovations
Gas traps
Crimpers
SeptaLinersGold sealsColumn nuts
A-line vials
Latest columnsFID jets
Sample cleanup
ADM FlowmeterStandards
Latest GC Supply Innovations Agilent RestrictedNovember 12, 201960
New Agilent Universal Fit GC Detector Jets
• Easier column installation and jet replacement reducing the risk of column damage
• Lubricant free threads reducing the risk of contamination
• Made of strong material reducing the risk of deforming
• Universally fits in both capillary column and packed column (adaptable) FID detectors
November 12, 201961 Latest GC Supply Innovations Agilent Restricted
Upcoming GC Seminars
Have an idea for a topic? Let us know.
November 12, 201962
How to combat the Helium Shortage: Making the Switch from Helium to Hydrogen or Nitrogen Carrier Gas
December 5, 2019 11:00am ET/8:00am PT
Optimizing Conditions for GC/MS Analyses
January 23, 2020 11:00am ET/8:00am PT
Plunge Deep into the World of Syringes: Simplifying GC Syringes and Sample Introduction
February 27, 2020 11:00am ET/8:00am PT
An Agilent Guide to SPME Analysis
March 26, 2020 11:00am ET/8:00am PT
Latest GC Supply Innovations Agilent Restricted
Contact Agilent Chemistries and Supplies Technical Support
1-800-227-9770 option 3, option 3:Option 1 for GC and GC/MS columns and suppliesOption 2 for LC and LC/MS columns and suppliesOption 3 for sample preparation, filtration, and QuEChERSOption 4 for spectroscopy suppliesOption 5 for chemical standardsAvailable in the USA and Canada 8–5, all time zones
[email protected]@[email protected]@[email protected]
63 Latest GC Supply Innovations Agilent RestrictedNovember 12, 2019