Experiment gone wrong? Transform it into a work of art!

Turn to page 9 to see all the winning entries for the 2015 Art in Microfluidics Award.

Online now: Biomicrofluidics special issue!

Selected Papers from the 2015 Annual Meeting of the AES Electrophoresis Society in

Salt Lake City, Utah (guest editors Nathan Swami and Michael Pycraft Hughes).

Read all the contributed papers at http://scitation.aip.org/content/aip/journal/bmf/10/3.

The 2016 AES Annual Meeting returns to San Francisco!

November 14-16, 2016

Look inside for a sneak peek at the preliminary program.

Late Breaking Poster Deadline: October 17, 2016.

Workshops are back for 2016! Watch your email for details coming soon.

AES 2016 Meeting Organizers

July 2016

Volume 21,

Issue 2 A E S N e w s l e t t e r

Inside this issue:

Annual Meeting Info 2-5

Electroporation Feature 6-7

SciX 2016 8

Art in Microfluidics

Award Winners 9

Many thanks to our sup-porters and friends for their generous contributions.

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questions about the Society

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Fatima Labeed

Associate Professor

University of Surrey

Biomedical Engineering

Guildford, England, UK

f.labeed@surrey.ac.uk

Lisa Flanagan

Associate Professor

Univ. California-Irvine

School of Medicine

Irvine, California, USA

lisa.flanagan@uci.edu

Abraham “Abe” P. Lee is the William J. Link Professor and Chair of the Biomedical Engineering Depart-ment with a courtesy appointment in Mechanical and Aerospace Engineering at the University of Califor-nia, Irvine (UCI). He is the Director of the NSF I/UCRC Center for Advanced Design & Manufacturing of Integrated Microfluidics. Prior to UCI, he was at the National Cancer Institute and a program manager in the Microsystems Technology Office at DARPA. Dr. Lee’s lab focuses on developing active integrated microfluidics and droplet-based platforms applied to point-of-care and molecular diagnostics, “smart” nanomedicine theranostics for early detection and treatment, sample preparation for cell sorting and enrich-ment, single cell processing and analysis, tissue engineering, and cell-based therapeutics. Dr. Lee serves as an associate editor for Lab on a Chip, and his research has contributed to the founding of several start-up companies. Professor Lee was awarded the 2009 Pioneers of Miniaturization Prize and is an elected fellow

of the American Institute of Medical and Biological Engineering and the American Society of Mechanical Engineers.

Elain Fu is an Assistant Professor in Bioengineering at Oregon State University. Prior to this, she spent three years as an Assistant Research Professor in Bioengineering at the University of Washington. Elain received a Sc.B. degree in Physics from Brown University, and M.S. and Ph.D. degrees in Physics from the University of Maryland, College Park. Her research focus has been microfluidics-based sensor develop-ment with the goal of using an understanding of the physics and chemistry of device operation to improve device performance for field applications. Recently, she has been active in the area of paper microfluidics. In particular, her lab develops tools for the automated manipulation of reagents in porous materials, as well as specific tests for high-sensitivity detection in low-resource settings for global health applications. She has published over 40 articles in peer-reviewed journals and is a co-inventor on multiple patents and patent

applications.

Utkan Demirci is an Associate Professor with tenure at the Stanford University School of Medicine, Palo Alto, CA. Before Stanford, he was an Associate Professor of Medicine and Health Sciences and Technol-ogy at the Harvard Medical School and Massachusetts Institute of Technology Health Sciences and Tech-nology division. He received his bachelor’s degree (summa cum laude) from the University of Michigan, Ann Arbor, his master’s degrees in Electrical Engineering in 2001 and in Management Science and Engi-neering in 2005, and his doctorate in Electrical Engineering in 2005, all from Stanford University. Dr. Demirci received the IEEE EMBS Early Career Award; IEEE EMBS Translational Science Award; NSF CAREER Award; Coulter Foundation Early Career Award; HMS-Young Investigator Award; and Chinese National Science Foundation International Young Scientist Award. In 2006, he was selected to TR-35 as one of the world’s top 35 young innovators under the age of 35 by the MIT Technology Review. Dr.

Demirci has over 110 peer-reviewed journal publications, 14 book chapters, 4 edited books, and holds more than 20 issued or

pending U.S. and international patents. He is the Editor-in-Chief of the journal Advanced Health Care Technologies.

John O’Neill studied Biochemistry as an undergraduate at the University of Oxford. This was followed by Ph.D. research on the mammalian circadian clock in the lab of Michael Hastings at the MRC Laboratory of Molecular Biology (LMB) in Cambridge. John continued to work on biological timing, next in plants and algae, with Andrew Millar at the University of Edinburgh. Finally, he worked with Akhilesh Reddy (University of Cambridge) on biological timing in human red blood cells. In 2013 he was recruited to be-come a group leader in the LMB's Cell Biology division. John has published 46 papers since 2003. The O'Neill lab's current research is focused on understanding the fundamental molecular mechanisms of the

cellular clockwork, and how it facilitates temporal regulation of biological function.

Marina F. Tavares received a B.S. degree in Chemistry from University of Sao Paulo, São Paulo, SP, Brazil in 1980, a M.Sc. in Analytical Chemistry from University of São Paulo (with Roberto Tokoro) in 1986 and a Ph.D. in Analytical Chemistry from Michigan State University, East Lansing, MI, U.S.A. in 1993 (with Victoria L. McGuffin). She joined the Institute of Chemistry at the University of Sao Paulo in 1997 as an assistant professor, became associate professor in 2003 and full professor in 2008. To date she has published 7 book chapters and 109 articles, and has participated in more than 180 symposia and con-ferences. Present research interests include separation science, physical chemistry and clinical metabolom-ics/peptid-omics. Projects are focused on modeling, simulation, method development and optimization of

conditions for the separation and analysis of molecules of clinical, forensic, nutritional, pharmaceutical, cosmetological, envi-

ronmental, and industrial importance using modern separation techniques.

AES Plenary Session: AES is excited to announce the following lineup of

speakers for this year’s Plenary Session!

P a g e 2 www.aesociety.org

AES MEETING PROGRAM — 2016

Meet our session chairs!

Electrokinetics for Biological Separation and Analysis Electrokinetics provides a significant tool for the separation and analysis of biomolecules, and has been adapted to the micro-

fluidic device format. The ability to study processes at the cell and molecular levels promises to provide a host of information

with benefits in the area of therapeutics and drug discovery. In this session, we invite papers describing biological separations

to probe chemical and biochemical responses at cellular and sub-cellular levels; proteomic technologies such as electrophoretic

protein separations; novel means of detecting and quantifying proteins or analyzing specific protein classes; proteomic analysis

of post-translational modifications; mass spectroscopic methods, and other related technologies.

Electrokinetics for Cellular Analysis & Separation Cellular electrokinetic analysis plays a vital role in understanding complex responses of cells, tissues, and organisms to stim-

uli and mutations. In order to fully understand the factors governing cellular response, new technologies are needed that pro-

vide quantitative information with high sensitivity and throughput. This session will focus on the development of analysis and

separation technologies. Of particular interest are advances in electrophoretic and dielectrophoretic cell separations, novel

means for cell detection, methods of analyzing cellular properties, and related technologies. Papers linking cellular analysis

and translational research will also be featured.

Electrokinetics for Sample Preparation Sample preparation is the link connecting the real-world with microfluidic analysis. For example, electrodes have been used

to induce electrothermal flows for on-chip single cell analysis and electrochemical assay development. Partially overlooked in

the past years, robust on-chip sample preparation must be further developed to enable practical applications such as environ-

mental monitoring and point-of-care clinical diagnostics. This session is aimed at presenting how electrokinetic technology,

including electrophoresis, electroosmosis, diffussiophoresis, etc., is enhancing sample preparation.

AES Award Session

Recognizing the career and accomplishments of Jean-Louis Viovy. See page 5 for complete details.

Chair: Adrienne Minerick

Chemical Engineering

Michigan Technological University

minerick@mtu.edu

Co-Chair: Erin A. Henslee

Mechanical Engineering Sciences

University of Surrey, UK

e.henslee@surrey.ac.uk

P a g e 3

Chair: Soumya Srivastava

Chemical and Materials Engineering

University of Idaho

srivastavask@uidaho.edu

Co-Chair: Tayloria Adams

Neurology

University of California Irvine

tayloria@uci.edu

Chair: Mark Hayes

Chemistry and Biochemistry

Arizona State University

mhayes@asu.edu

Co-Chair: Michael J. Heller

Nanoengineering

University of California San Diego

mjheller@ucsd.edu

Chair: Rodrigo Martinez-Duarte

Mechanical Engineering

Clemson University

rodrigm@clemson.edu

Co-Chair: Christa N. Hestekin

Chemical Engineering

University of Arkansas

chesteki@uark.edu

P a g e 3 Electrokinetics: Advancing the Fundamentals (Sessions I and II)

Electrokinetics describes interactions between electrical fields and particles to produce colloidal particle motion within a medium,

which may be a fluid, or a porous or fibrous medium. A detailed understanding of particle-particle electrostatic forces is particu-

larly relevant, and both experimental and simulation approaches are required to advance understanding of electrokinetic science.

Therefore, contributions with novel approaches related to fundamental micro/nanoscale electrokinetic principles, modeling, and

experimental studies are welcome. Topics will include electrokinetic networks, biosensors, chemical assays, electrophoretic mo-

bility and electro-optics.

Session I Co-Chairs

Session II Co-Chairs

Soft Matter Electrokinetics: Particles, Drops, and Bubbles

Electric fields can be used to concentrate, pattern, and sort various sized colloidal particles for directed assembly of nanoparticles,

or for droplet deformation. Electrokinetic phenomena such as electrophoresis, electroosmosis, dielectrophoresis, electrothermal

flows, induced charge electrokinetics, and electrohydrodynamic, are at play during these processes leading to direct or indirect

manipulation of particles, droplets, and bubbles. Fundamental and applied papers pertaining to the assembly or transport of parti-

cles, droplets and/or bubbles using electrokinetics or related physical phenomena, and microfluidic colloidal crystallization will be

featured.

Electrokinetics and Microfluidics for Biomolecular Analysis

Remarkable progress has been made in the fabrication of micro/nanoscale devices for the manipulation and detection of organ-

isms and biomolecules. This session focuses on integration and detection aspects related to the emerging concept of a ‘chip-in-a-

lab’ as well as the more established ‘lab-on-a-chip’ systems includeing (but not limited to) platforms for uni/multicellular analy-

sis, immunosensors, electrochemical sensors, and other spectroscopic and separation tools in a microchip format. Experimental

and theoretical papers dealing with micro/nanoscale systems and issues related to biochemistry, cellular, and systems biology will

be featured.

AES Plenary Session See page 2 for this year’s lineup of plenary speakers. Chaired by the AES 2016 Meeting Organizers, Lisa Flanagan and Fatima Labeed (see page 1 for contact information).

P a g e 4 www.aesociety.org

Chair: Kyle Bishop

Chemical Engineering

Pennsylvania State University

kjmbishop@engr.psu.edu

Co-Chair: Michael Sano

School of Medicine

Stanford University

mikesano@stanford.edu

Chair: Michael Hughes

Biomedical Engineering

University of Surrey, UK

m.hughes@surrey.ac.uk

Co-Chair: Nathan Swami

Electrical & Computer Engineering

University of Virginia

nswami@virginia.edu

Chair: Christopher Wirth

Chemical & Biomedical Engineering

Cleveland State University

c.wirth@csuohio.edu

Co-Chair: Stuart J. Williams

Mechanical Engineering

University of Louisville

stuart.williams@louisville.edu

Chair: Zachary R. Gagnon

Chemical & Biomolecular Engineering

Johns Hopkins University

zgagnon1@jhmi.edu

Co-Chair: Sagnik Basuray

Chemical, Biological & Pharmaceutical

Engineering

New Jersey Institute of Technology

sagnik.basuray@njit.edu

P a g e 5

Special Proceedings Issue of ELECTROPHORESIS

Once again, AES is excited to announce that we are teaming up with the journal ELECTROPHORESIS to publish a special proceedings issue highlighting manuscripts associated with work presented at the Annual Meeting. We will follow an accelerated timeline with a manuscript submission deadline of December 15, 2016, with a targeted publication date in May 2017. Please sub-mit manuscripts electronically at: http://mc.manuscriptcentral.com/elpho indicating that they are intended for this special proceedings issue, or contact one of the co-editors directly for more information (Fatima Labeed, Lisa Flanagan, and Blanca H. Lapizco-Encinas). All submissions will be subject to peer review in accordance with the

standard journal policies. Please take advantage of this exclusive opportunity to share your latest discoveries!

Jean-Louis Viovy

Professor Jean-Louis Viovy, a Research Director at the Institut Curie in Paris, France, has been hon-ored with the Lifetime Achievement Award by the AES Electrophoresis Society at the Society’s annual meeting to be held in San Francisco, CA November 14-16. The Lifetime Achievement Award recognizes individuals who have made exceptional contributions to the fields of electropho-resis, electrokinetics and related areas. The governing board of the society elected Jean-Louis “for his outstanding work on capillary electrophoresis, including chip electrophoresis, of DNA.” Previous

winners of this award include Neil Ivory, Pier Giorgio Righetti, Nancy Stellwagen, and Kelvin Lee.

Prof. Viovy is Research Director at the CNRS (Centre National de la Recherche Scientifique) in France. Since 1999, he has lead within the Institut Curie (UMR168) the MMBM team (Macromolecules and Microsystems in Biology and Medicine) comprising about 25 researchers (permanent, Engineers, PhD students and postdocs). He was awarded the Bronze Medal of the CNRS (1983), the Polymer Prize of the French Chemical Society (1996), the Philip Morris Scientific

Prize in 1996 (with F. Caron, D. Chatenay and R. Lavery), and two OSEO Entrepreneurship Awards in 2004 and 2005. He is author or co-author of more than 220 articles (h-factor 45) and 22 patents. He has been involved in seven European projects, including 3 as coordinator. He was recently granted ERC funding (advanced grant) on the topic of development of artificial or-gans. He is member of the board of the Chemical and Biological Microsystems Society, which organizes the MicroTAS confer-

ence, and was chairman of this conference in 2007 in Paris.

Please join us for a session of inspiring internationally renowned speakers

to celebrate Prof. Viovy’s contributions!

AES Award Session: AES invites you to join us in celebrating the distin-

guished contributions of Prof. Jean-Louis Viovy

Madhavi Krishnan

Departments of Chemistry

and Physics

University of

Zurich

Switzerland

James Landers

Department of Chemistry

University of

Virginia

USA

Amit Meller

Department of Biomedical

Engineering

Technion – Israel Institute

of Technology

Israel

Yoshinobu Baba

Departments of Applied Chemistry and Advanced

Medical Science

Nagoya University

Japan

P a g e 6 www.aesociety.org

Microfluidic Electroporation

By Tao Geng1 and Chang Lu2 1Environmental Molecular Sciences Laboratory, Pacific

Northwest National Laboratory, Richland, WA 99354, USA 2Department of Chemical Engineering, Virginia Tech, Blacks-

burg, VA 24061, USA

Electroporation, also termed as electropermeabilization, is a robust physical method for breaking cell membranes to ei-ther release intracellular components out of cells or intro-duce exogenous molecules into cells.1-3 When an electric field is applied to a cell, cell membrane charges like a ca-pacitor, as it has several orders of magnitude higher con-ductivity than cytoplasm and extracellular medium. Thus, a potential difference is formed across the intact cell mem-brane within an extremely short charging time (on the scale of microseconds). As the transmembrane potential (ΔψE) exceeds a critical threshold (typically 0.2 to 1 V irrespec-tive of cell types), electroporation occurs with nanoscale eletropores created within the plasma membrane via the localized structural rearrangements of lipid molecules.1,2 Assuming that the cell is a sphere and the membrane charg-ing time is much shorter than the electric field duration, the ΔψE induced by an external electric field can be described using the following equation: ΔψE = 0.75 g(λ) a E cosθ where g(λ) is a function of the con-ductivities of cytoplasm, cell mem-brane and extracellular buffer, a is the cell diameter, E is the electric field intensity, and θ is the angle between the direction of the electric field E and the normal from the cell center to a point on the membrane

surface.

Conventional electroporation is implemented by exerting short elec-tric pulses of defined duration and intensity to a cuvette with elec-trodes fabricated out of aluminum, stainless steel, platinum or graphite, and arranged in a plate-to-plate manner.4 However, these systems require high voltage input and suf-fer from adverse environmental conditions such as electric field distortion, local pH variation, metal ion dissolution and excess heat generation, leading to low electro-poration efficiency. Microfluidic electroporation systems overcome

many drawbacks of bench-scale electroporators owing to its unique characteristics of miniaturization and integration.5-7 First, microfluidic electroporation devices are fabricated with standard microfabrication techniques, and hence a wide spectrum of microelectrodes can be incorporated into the microchips to generate the field necessary for electropo-ration. By shrinking the distance between microelectrodes to a few tens of µm8-10 or creating physical constraints with subcellular dimensions11-13, the required voltage is dramati-cally decreased to a few volts. Second, the electroporation microchips provide relatively more uniform electric field distribution, favorable fluidic and chemical environment, and rapid heat dissipation in small-volume microstructures. Third, individual cells could be manipulated on chips to probe single-cell dynamics and identify cell heterogeneity. The miniaturization of the systems also makes them very suitable for assays involving rare cell populations and ex-pensive reagents due to the substantially reduced sample consumption. Fourth, the flow-through format of the micro-fluidic electroporation also allows the continuous process-ing of large cell populations with high throughput to obtain statistically meaningful results at the single-cell level or produce in a large volume.11,13-17 Fifth, the utilization of transparent materials (e.g., polydimethylsiloxane and glass) for microchips allows in situ observation and real-time

Figure 1. Schematic of microfluidic electroporation methods. (a) Electrode incorporation and configuration: interdigitated 2D planar electrodes incorporated in a microchamber; (b) Channel geometry variation and constriction structures: a fluidic channel comprising a number of alternating wide and narrow sections where cells undergo electroporation while flowing through the narrow section; (c) Hydrodynamics-enhanced electroporation: hydrodynamic focusing for single-cell electroporation; (d) Compartmentalized electropo-

ration: single-cell electroporation within a microfluidic droplet surrounded by oil.

P a g e 7

monitoring of electroporation process using fluorescent probes, which facilitates the exploration of electroporation mechanism. Finally, microfluidic electroporation can be integrated with other analytical processes such as dielectro-phoresis, electrophoresis, electroosmosis, and hybridization to implement a total analysis analytical system, which is especially critical to intracellular content analysis.18-32 Based on the strategies used to facilitate electroporation at the microscale, microfluidic electroporation methods are divided into five categories: (1) electrode incorporation and configuration, (2) channel geometry variation and constric-tion structures, (3) hydrodynamics-enhanced electropora-tion, (4) compartmentalized electroporation, and (5) other miscellaneous methods. Figure 1 demonstrates representa-tive microfluidic electroporation methods. The fusion of microfluidics with electroporation will benefit biotechnol-ogy and life sciences in broad realm including cell therapy, electrochemotherapy, large-scale functional screening of

genomes, and cell biology studies.

References

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Bioenerg. 1996, 41, 135-160.

(2) Teissie, J.; Golzio, M.; Rols, M. P. Biochim. Biophys.

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Anal. Bioanal. Chem. 2006, 385, 474-485.

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(12) Munce, N. R.; Li, J.; Herman, P. R.; Lilge, L. Anal.

Chem. 2004, 76, 4983-4989.

(13) Khine, M.; Lau, A.; Ionescu-Zanetti, C.; Seo, J.; Lee,

L. P. Lab Chip 2005, 5, 38-43.

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242-249.

(15) Khine, M.; Ionescu-Zanetti, C.; Blatz, A.; Wang, L. P.;

Lee, L. P. Lab Chip 2007, 7, 457-462.

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8, 62-67.

(17) Valley, J. K.; Neale, S.; Hsu, H. Y.; Ohta, A. T.; Jam-

shidi, A.; Wu, M. C. Lab Chip 2009, 9, 1714-1720.

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technol. 1998, 16, 541-546.

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Chip 2011, 11, 2417-2423.

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C.; Lim, T. M. Sens. Actuators B 2006, 113, 944-955.

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Tao Geng Chang Lu

The SciX Conference is hosted by The Federation of Analytical Chemistry and Spectroscopy Societies (FACSS),

which focuses on analytical chemistry and related sciences. For the seventh year, AES Electrophoresis Society is co-

organizing sessions with FACSS to include such topics as fundamentals and electrokinetic theory, micro– and nanoflu-

idics, gradient techniques, DNA separations, genomics, proteomics, and other biomolecular analysis. The programming

chair for the entire SciX meeting this year is our own Alexandra Ros from Arizona State University. The AES session

organizers are Ryan Kelly from the Pacific Northwest National Laboratory and Jason Dwyer from the University of

Rhode Island.

July 31: Deadline for Late Breaking and Student Poster Abstracts; last day to edit submitted abstracts.

September 1: Advanced registration rate deadline.

Networking Opportunities: One of the unique features of this meeting is the emphasis on networking. The conference

begins with a welcome mixer on Sunday evening. Monday night features a reception to foster interactions between aca-

demia and industry. A dinner party for all attendees is held on Wednesday evening. Numerous activities are planned for

smaller groups throughout the week. The business office collects resumes and puts attendees in contact with companies

that are recruiting at the conference.

Student Poster Session: AES is sponsoring a student / postdoc poster session at SciX. First place prize is $250 and Sec-

ond place is $100. Please select the “AES Electrophoresis Society Student Poster Award” box when on the submission

page and uploading your poster abstract. There are numerous opportunities for students to receive discounted or free

conference registration and travel reimbursements. Visit the awards section of the conference website for additional de-

tails.

2016 AES Mid-Career Award

AES is pleased to announce that Dr. Amy Herr, Lester John and Dewar Lloyd Distinguished Pro-

fessor in the Department of Bioengineering at the University of California at Berkeley, is the recipi-

ent of the 2016 AES Mid-Career Award. Amy’s plenary talk on Thursday, September 22nd is ti-

tled: “Electrophoretic Cytometry: Targeted Proteomics in Single Cells.” The AES Mid-Career

Award is presented to individuals for their exceptional contributions to the field of electrophoresis,

microfluidics, and related areas who are currently in the middle of their career.

P a g e 8 www.aesociety.org

P a g e 9

2015 Art in Microfluidics Winners

Congratulations to the winning entries of the Art in Microfluidics Award competition, jointly sponsored by AIP Biomicroflu-idics and AES, and held in conjunction with the 2015 AES Annual Meeting. The talented artists behind these works uniquely excelled at expressing their scientific research in creative and unexpected ways. Even experiments gone wrong can become works of art! Winners were selected in both image and video categories. The first place award for each category was $300 + $100 for conference travel/registration. Both Runners Up and Honorable Mentions were awarded $100 + $100 for confer-

ence/travel. Many thanks to our friends at AIP Biomicrofluidics for generously co-sponsoring these awards!

Best Image: Mario Saucedo (RIT)

Miscarried Separation in a SpiderChannel Microdevice

Runner Up Image: Avanish Mishra (Purdue)

A Bacteria Flower

Honorable Mention Image: Ali Rohani (Virginia)

Conductivity Gradient-enhanced Dielectrophoresis

Best Video:

Tayloria Adams (UC, Irvine)

Dielectrophoretic Patterning of

Sickle Cells

https://youtu.be/UFJGmHnHtU0

Runner Up Video:

Renny E. Fernandez (SMU)

Dielectrophoretic Profiles of Cells

Created by Pt Black Threads

https://youtu.be/XwoJg3YV0pU

Honorable Mention Video:

Aashish Priye (Sandia)

Convective PCR

https://youtu.be/-zWgg7yo1ak

Image Category

Video Category