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Gene Transfer | Electroporation

... for Better ResultsUse the Gene Pulser MXcell™ electroporation system to quickly optimize conditions for deliveringmolecules efficiently into mammalian cells — especially into primary and difficult-to-transfect cells.This high-throughput system uses disposable 12-, 24-, and 96-well plates and can be used fortransfecting siRNA, plasmid DNA, and other molecules directly into the nucleus.

Optimize Your Research Timen Use a preset optimization protocol or a gradient protocol to decrease programming timen Process an entire multiwell plate in 2 minutes or less

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Edited by Simon Hughes Tumour Biology Laboratory, Cancer Research UK Clinical Centre and Adrian Moody, AstraZeneca

PCR is probably the most widely-used technique in molecular biology today, with new variantsappearing on a frequent basis. Many of the new methods offer substantial benefits over protocols thathave previously been covered in original papers and lab manuals. PCR: Methods Express describes thelatest PCR-based methodologies and approaches and provides the most up-to-date practical adviceon how to tackle a broad range of biological problems using these techniques.PCR: Methods Express is a comprehensive research guide; every chapter discusses the merits and limitations of variousapproaches and then provides selected tried-and-tested protocols with useful hints and tips for success, along with strategiesfor optimization. This book is an essential laboratory manual for researchers in all life science fields, and at all levels frompostgraduate student to principal investigator.

2007, 400pp., full-color sectionPaperback: $75.00 ISBN 978-1-904842-28-6Hardback: $125.00 ISBN 978-1-904842-29-3

CONTENTS1. Polymerase Chain Reaction:

From the beginningSimon Hughes, Tumour BiologyLaboratory, Cancer Research UKClinical Centre, London

2. Polymerases for PCRMeg Martel, Simon Baker andSimon May, ABGene

3. Approaches for PCR cleanupJohn DeMartino and Paul Pickering,Invitrogen

4. A detailed guide to quantitativeRT-PCRPete Kaiser, Institute for AnimalHealth, Compton, Newbury

5. Identification of genomicmicrodeletions andmicroduplications usingquantitative PCRRosanna Weksburg, The Hospital forSick Children, Toronto; SimonHughes, Tumour Biology Laboratory,London; Jeremy Squire, OntarioCancer Institute, Toronto

6. PCR multiplexesJuan Sanchez, Claus Borsting andNiels Morling, all at the Institute ofForensic Medicine, KøbenhavnsUniversity

7. Use of PCR for DNAmethylation analysisMario F. Fraga and Manel Esteller,both at the Spanish National ScienceCentre

8. Forensic genetic DNA typingwith PCR based methodsClaus Borsting, Juan Sanchez andNiels Morling, all at the Institute ofForensic Medicine, KøbenhavnsUniversity

9. Robust and unique PCR forgenotyping applicationXiangning Chen, Virginia Institutefor Psychiatric and BehavioralGenetics

10.Rapid generation of genetargeting constructs by PCRTrevor Wilson, Antonietta Giudiceand Paul Hertzog, all at the MonashInstitute of Medical Research

11.Using PCR and linkagemapping to identify singlegenes and QTLs for livestocktraitsJill Maddox, Centre for AnimalBiotechnology, School of VeterinaryScience, University of Melbourne

12.Preimplantation geneticdiagnosis (PGD) of monogenicdisease: PCR-based methodsfor the identification ofmutations in single cellsDagan Wells, Yale University MedicalSchool

13.PCR and diagnosis ofinfectious diseasesRichard Rothman, Department ofEmergency Medicine, Johns HopkinsUniversity

14.Efficient PCR-basedmutagenesis method applicab-le to diverse mutagenesisstrategies using type IIsrestriction enzymeJae-Kyun Ko, RobertWood JohnsonMedical School, University ofMedicine and Dentistry of New Jersey

15.Construction of long DNAmolecules from multiplefragments using PCRNikolai Shevchuk and AntonBryksin, Center for Cancer andImmunology Research, Washington

16.PCR-based whole genomeamplificationSimon Hughes, Tumour BiologyLaboratory, Cancer Research UKClinical Centre, London; NonaArneson and Susan Done, both at the Division of ExperimentalTherapeutics, Ontario CancerInstitute, Toronto

17.PCR and microarraysIan Giddings, Cancer ResearchUK DNA Microarray Facility,Institute of Cancer Research

18.Inverse PCR-based RFLP foridentifying low-level mutationsin tumorsMike Makrigiorgos, DanaFarber-Brigham and Women’ sCancer Center

List of suppliers; index

To order or request additional information:Call: 1-800-843-4388 (Continental US and Canada) Fax: 516-422-4097Email: USorders@scionpublishing.com WWW Site: www.scionpublishing.comWrite: Scion Publishing Ltd., 500 Sunnyside Blvd., Woodbury, NY 11797-2924

Methods Express• protocols in step-by-step detail• comprehensive troubleshooting • example data as benchmarks• key references to further readingSeries Editor: B. David Hames,University of Leeds

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Now you can see results of the finest methods in action with new online movies inCSH Protocols.

The November issue of CSH Protocols features methods for tracking RNA and protein molecules in both cells and organisms, and introduces new multimediacontent made possible by online presentation: movies showing examples of thesetechniques in action.

Read—and see—these newly featured protocols online at www.cshprotocols.org:

• Photoactivation-Based Labeling and In Vivo Tracking of RNAMolecules in the Nucleus

• Selection of Appropriate Imaging Equipment and Methodology for Live Cell Imaging in Drosophila

CSH Protocols offers cutting edge and classic protocols in molecular and cell biologyfrom laboratories worldwide, along with methods from Cold Spring HarborLaboratory’s renowned manuals and on-site courses. Free trials and discounted subscriptions are available for a limited time.

CSH Protocols is created by Cold Spring Harbor Laboratory Press in associationwith HighWire Press of Stanford University.

For pricing information or to request a free trial visit the website or: Phone:1-800-843-4388 (Continental US and Canada) or 516-422-4100 (all other locations)FAX: 516-422-4097 E-mail: cshpress@cshl.edu Write: Cold Spring Harbor Laboratory Press, 500 Sunnyside Blvd., Woodbury, NY 11797-2924

Subject Coverage

Antibodies

Bioinformatics/Genomics

Cell Biology

Chromatography

Computational Biology

DNA Delivery/GeneTransfer

Electrophoresis

Genetics

High ThroughputAnalysis

Imaging/Microscopy

Immunology

Laboratory Organisms

Molecular Biology

Neuroscience

Plant Biology

Polymerase ChainReaction (PCR)

Proteins and Proteomics

RNA Interference(RNAi)/siRNA

Stem Cells

Transgenic Technology

Cold Spring Harbor Protocols • Executive Editor: Dr. Michael RonemusISSN 1559-6095 / online, monthly • Available exclusively via institutional site license

75 years at the frontiers of science: www.grc.org

Gordon Research Conferences

THE SCIENCE OF VIRAL VECTORS FOR GENE THERAPY: The Host Response to Viral Infection

March 2-7, 2008Crowne Plaza, Ventura, CA

Gene transfer has the potential to treat innumerable genetic and acquired diseases. Although viral vectors remain the most efficient means of gene delivery, further progress in understanding the fundamental biology of viruses, their special relationships with the host including mechanisms of infection, persistence, gene expression and immune evasion is required for successful virus-mediated gene therapy. Thus the focus of this meeting is to examine the host response, in particular the immune response, to viral infection and how to regulate it. The meeting will bring together immu-nologists, virologists and gene therapists to discuss these important aspects of gene therapy. The gathering of experts in fields of gene therapy, basic virology and immunology offers a unique opportu-nity to find solutions to new challenges in this field of regulating the host immune response to viral gene transfer. The design of this conference is to provide a detailed scientific overview of specific aspects of virus biology that relate to the immune response and the use of this knowledge to enhance effective gene delivery, resulting in successful gene therapy. Each session will have speakers with expertise in viral vectors, the host response to viral infection and/or immunology. In addition to the invited speakers per session, additional speakers will be selected from the submitted abstracts. There will also be travel grants available for graduate students and post-doctoral fellows.

TOPICS & SPEAKERS (discussion leaders noted in italics):

Immune Response to Viral VecorsPaul Robbins / Kathy High

Biodistribution and TargetingJohn Engelhardt / Jude Samulski / Renata Pasqualini / Wadih Arap / Richard Vile / David Schaffer

Viral Entry and Nuclear TranslocationJude Samulski / John Engelhardt / Arun Srivastava / Mark Kay

Host Cell Response to Viral InfectionJoseph Glorioso / Karen Mossman / Erik Falck-Pedersen / Paola Grandi

Innate Immune Response to Viral InfectionJames Wilson / Daniel Muruve / Glen Nemerow / Yiping Yang

Adaptive Immune Response to Viral InfectionLuigi Naldini / Andrea Amalfitano / James Wilson / Luca Guidotti

Approaches to Blocking the Immune Response to Viral InfectionMalcolm Brenner / Luigi Naldini / Maria Grazia Roncarolo / Roland Herzog

Suppression of the Immune Response by Gene TransferMaria Grazia Roncarolo / Paul Robbins / David Scott / Garrison Fathman

Stimulation of the Immune Response by Gene TransferGarrison Fathman / Carl June / Glenn Dranoff / Malcolm Brenner / Marjorie Robert-Guroff

GRC is holding over 190 meetings in 2008. One upcoming meeting that may be of particular interest to Genes & Development readers is listed below.