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Doc. No. 031951-04 Page 1 of 2 August 2007

ASRS 300CSRS 300

QUICKSTART

A. Hydrating the Suppressor

1. Hydrating the suppressor ensures that the ion exchange membranes are in a swollen form for proper operation.A 20 minute static step is recommended during first time installation to ensure complete hydration.

2. Using a disposable plastic syringe and the 10-32 Luer adapter (P/N 046888), push approximately 3 mL (SRS 4-mm) or 0.75 mL(SRS 2-mm) of degassed DI water through the ELUENT OUT port. Using a disposable syringe and the

e a d T h i s F i r s

1/4-28 Luer adapter (P/N 024305), push 5 mL (SRS 4-mm) or 2 mL (SRS 2-mm) of degassed DI water through the REGEN IN port, according to Figure 1.

NOTE

Step 1 can be accomplished by installing the suppressor in the system in the recycle mode, bypassing the guard and analytical columns, and pumping 5 mL (SRS 4-mm) or 2 mL (SRS 2-mm) of deionized water through the suppressor.

P/N Description

1CC

016388 1 cc plastic syringe024305 1/4-28 Luer adapter 046888 10-32 Luer adapter 042627 10-32 Union043275 10-32 Bolt043276 10-32 Double Cone Ferrule

Figure 1Hydrating the Suppressor

3. Allow the suppressor to sit for approximately 20 minutes to fully hydrate the suppressor screens and membranes.

WARNING

Do not install a luer adapter directly into a SRS 300 port. Connect a luer adapter to a union and use two 10-32 bolt and ferrules with a few centimeters of tubing to connect to the SRS ports.

B. Back Pressure Coil Pressure Test

1. Disconnect the eluent line from the injection valve to the column at the column inlet.

2. Connect the eluent line from the injection valve directly to the detector cell inlet withthe recommended number of back pressure coils attached for your application (see thetable below). Turn the pump on at your application flow rate. After 2 to 3 minutes of equilibration record pressure P 1.

3. Disconnect the back pressure coils and with the pump on measure the system pressureP2.

4-mm Chromatography 2-mm Chromatography____________

1.0 mL/min. = 2 black backpressure coils 0.25 mL/min. = 2 red backpressure coils(P/N 045877) (P/N 045878)

2.0 mL/min. = 1 black backpressure coil 0.50 mL/min. = 1 red backpressure coil(P/N 045877) (P/N 045878)

ELUENTOUT

REGENIN

R EGEN OU TEL U EN TIN

1.0 cc syringe

Luer adapter Bolt andFerrule Union

D IO NEX AN I O N SEL F - R EG ENERATING

SU P PR E S SOR A SR SULTRA 4-mm1

DI H2O

DI H2ON 0 P / 6 1 56

Waste

Cell

InjectionValve

Coil

#2Coi l

#1

Waste

Cell

InjectionValve

Coil

#2Coi l

#1

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Wa s t e

InjectionValveInjectionValve

Cell

4. The correct operating pressure range for the backpressure coil being tested is

SRS 300 P 1 - P2 = 3040 psi

If the pressure is greater than 40 psi, then trim the back pressure coil and repeat step 2and 3 to achieve 30-40 psi in step 4.

If it is less than 30 psi, then add more tubing to achieve 30 - 40 psi.

C. Quick Back Pressure Check Refer to section 3.2.3. in SRS 300 Product Manual P/N 031956

D. Column Purging

1. If the column is new, purge column to waste with eluent for at least 10 minutes before connecting the column to thesuppressor flow path.

2. Connect the suppressor to your system in the recycle mode according to Figure 2.

NOTE

Recycle mode is recommended for all routine applications where solvent is not used in the eluent. Use theexternal water mode for all applications where solvent (< 40% v/v) is used in the eluent, analysis of borated waters or analysis of samples with complex matrices. Refer to the suppressor manual for moredetails.

Recommended tubing for various column formats2-mm i.d., 10-32, 0.005" i.d. PEEK Liquid Line3-mm i.d., 10-32, 0.005" i.d. PEEK Liquid Line4-mm i.d., 10-32, 0.010" i.d. PEEK Liquid Line5-mm i.d., 10-32, 0.010" i.d. PEEK Liquid Line

10-32

1/4-28REGEN OUT

ELUENT IN

10-32

1/4-28

ELUEN T OUT

REGEN IN

InjectionValve

GuardColumn

1/4-28

1/4-28

GasSeparator

Waste

TubeWaste

AnalyticalColumn

10-32

Cell

10-32 10 -32 10 -321/4-28

10-32 or1/4-28

10-32 or1/4-28

10-32 or1/4-28

Signal toConductivityDetector

DIONEXSELF-REGENERATING

SUPPRESSORSRSU LTRA IIP/N0xxxxx

1/428 Tubing i.d.

Backpressure Coils

1/428 Tubing i.d.

Figure 2Connecting the Suppressor to the System in Recycle Mode (Optional)

E. Connecting Suppressor and Power Settings

Connect the suppressor to the power supply and set the power setting to the recommended current as defined for your eluentconditions. See suppressor manual for more details on power settings.

CAUTION

The Recommended Maximum Current Setting for the SRS 300 2-mm is 100 mA.s a general operation precaution, never apply current to the SRS 300 without eluent or water regenerant

lowing through the eluent and regenerant channel of the SRS 300.