5. SKHep1C3 72h 6. Lipofectamine 72h 7. anti-miR-193a 50 nM 72h
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5. SKHep1C3 72h 6. Lipofectamine 72h 7. anti-miR-193a 50 nM 72h 8. anti-miR- 193a 100 nM 72h 1. SKHep1C3 48h 2. Lipofectamine 48h 3. anti-miR-193a 50 nM 48h 4. anti-miR-193a 100 nM 48h 1. Lipofectamine 48h 2. 50 nM miR-193a 48h 3. 100 nM miR-193a 48h 4. SKHep1C3 72h 5. Lipofectamine 72h 6. 50 nM miR-193a 72h 7. 100 nM miR-193a 72h 1 2 3 4 5 6 7 1 2 3 4 5 6 7 8 Loading control (Coomassie staining) Loading control (Coomassie staining) SKHep1C3 T 2 = 72h 5. HA22T/VGH 6. Lipofectamine 7. 50 nM anti-miR-193a 8. 100 nM anti-miR- 193a T 1 = 48h 1. HA22T/VGH 2. Lipofectamine 3. 50 nM anti-miR-193a 4. 100 nM anti-miR- 193a 1 2 3 4 5 6 7 8 1. HA22T/VGH 2. Lipofectamine 3. 50 nM miR-193a 4. 100 nM miR-193a 48 h 72 h 1 2 3 4 Loading control (Coomassie staining) Loading control (Coomassie staining) HA22T/VGH Additional file 6 kDa 110 - 60 - 50 - 40 - kDa 110 - 60- 50 - 40 - 30 - kDa 110 - 60 - 50 - 1 2 3 4 kDa 110 - 60 - 50 - 40 - kDa 110 - 60 - 50 - 40 - A B Loading controls relative to Figs. 2A, 2B, 3A and 3B. Coomassie staining of proteins loaded on polyacrylamide gels 8% (panel A, left) and 4-12% pre-cast gels (panel A, right and panel B). The staining highlights constant amounts of total proteins loaded for each gel.
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A. HA22T/VGH. Loading control ( Coomassie staining). Loading control (Coomassie staining ). kDa 110 - 60 - 50 - 40 -. kDa 110 - 60 - 50 - 40 -. 48 h. 1 2 3 4 5 6 7 8. 1 2 3 4. T 2 = 72h 5. HA22T/VGH - PowerPoint PPT Presentation
Transcript of 5. SKHep1C3 72h 6. Lipofectamine 72h 7. anti-miR-193a 50 nM 72h
5. SKHep1C3 72h6. Lipofectamine 72h7. anti-miR-193a 50 nM 72h 8. anti-miR- 193a 100 nM 72h
1. SKHep1C3 48h2. Lipofectamine 48h3. anti-miR-193a 50 nM 48h4. anti-miR-193a 100 nM 48h
1. Lipofectamine 48h2. 50 nM miR-193a 48h3. 100 nM miR-193a 48h4. SKHep1C3 72h5. Lipofectamine 72h6. 50 nM miR-193a 72h7. 100 nM miR-193a 72h
1 2 3 4 5 6 7
1 2 3 4 5 6 7 8
Loading control (Coomassie staining)
Loading control (Coomassie staining)
SKHep1C3
T2= 72h5. HA22T/VGH 6. Lipofectamine7. 50 nM anti-miR-193a8. 100 nM anti-miR-193a
T1= 48h1. HA22T/VGH2. Lipofectamine3. 50 nM anti-miR-193a4. 100 nM anti-miR-193a
1 2 3 4 5 6 7 8
1. HA22T/VGH2. Lipofectamine3. 50 nM miR-193a4. 100 nM miR-193a
48 h
72 h
1 2 3 4
Loading control (Coomassie staining)
Loading control (Coomassie staining)
HA22T/VGH
Additional file 6
kDa110 -
60 -50 - 40 -
kDa110 -
60-
50 -
40 -30 -
kDa110 -
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1 2 3 4
kDa110 -
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kDa110 -
60 -50 - 40 -
A
B
Loading controls relative to Figs. 2A, 2B, 3A and 3B. Coomassie staining of proteins loaded on polyacrylamide gels 8% (panel A, left) and 4-12% pre-cast gels (panel A, right and panel B). The staining highlights constant amounts of total proteins loaded for each gel.
