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    Application of UltrasoundTechnology in Foods

    Valente B. AlvarezFood Industries Center

    Department of Food Science and

    Technology

    V.B. Alvarez/Ultrasound/10-2007

    Application of UltrasoundTechnology in Foods

    Contents

    Ultrasound principle

    Ultrasound applications

    Microbial inactivation

    Applications

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    Basic Principle (Knorr, 2004, Piyasena et.al., 2003)

    When sound energy passes to the mediumresulting in a continuous wave-type motion,longitudinal waves are generated

    The motion creates alternative compressionand rarefaction of the medium particles

    Depending on the frequency and amplitude,

    a number of physical, chemical andbiochemical effects are observed.

    V.B. Alvarez/Ultrasound/10-2007

    Ultrasound of interest

    20 kHz (human range) to 100sof MHz

    Able to penetrate solid andliquid materials

    Used widely in medicine (fetusexamination), engineering(inspection of materials), andagriculture (measurement ofbackfat on livestock)

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    Energy of sound field

    Characterized by

    Sound power (W)

    Sound intensity (W/m2)

    Sound energy density (Ws/m3)

    Classified as

    Lower energy

    Low frequency low amplitude

    High frequency low amplitude

    Higher energy

    Low frequency high amplitude

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    How ultrasound can benefit foodindustries? (McClements, 1995)

    New analytical techniques to study complexfood materials and to monitor properties offoods during processing

    Relatively inexpensive ultrasonicinstrumentation

    Fully automated, rapid and precise

    measurements, non-destructive, non-invasiveand online applications

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    Effects during Food Processing

    Generation of heat

    Cavitation (implosion of gas bubbles)

    Caused by a rapid change of heating to5500C and pressure increase to 50 MPa(Leighton, 1998)

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    Low energy ultrasoundapplications

    Intensities lower than 1 W/cm2 andfrequencies higher than 100 kHz

    Used for non-invasive detection (processcontrol) and for characterizing

    physicochemical properties of foodmaterials (product control)

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    Low energy ultrasound

    applications (contd..)

    Stimulation of activity of living cells

    Surface cleaning of foods

    Effects on enzymes

    Ultrasonically assisted extraction,crystallization, emulsification, filtration,

    drying and freezing as well astenderization of meat

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    High energy ultrasoundapplications

    Intensities higher than 1 W/cm2 andfrequencies between 20 and 100 kHz

    Applications include, degassing of liquidfoods, induction of oxidation/reductionreactions, extraction of enzymes andproteins, induction of nucleation for

    crystallization Inactivation of heat resistant enzymes

    Inactivation of microorganisms

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    V.B. Alvarez/Ultrasound/10-2007(Povey, 1998)

    V.B. Alvarez/Ultrasound/10-2007

    Transmission electronmicrograph showing aprostate cancer cellimmediately after exposureto ultrasound. Image hasbeen color enhanced toshow to the spot where thecell membrane has beenremoved.

    Image courtesy of RobynSchlicher, Robert Apkarianand Mark Baran

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    Sanitation of food processing

    equipment-fouling (Withers, 1996)

    Ultrasonic sensor for measurement of fouling inpipelines

    Pulse-echo technique - detect fouling films as thin as0.1mm

    Transmission technique-limit of 0.5mm

    Useful in tube-in-tube heat exchangers and other

    tubular systems

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    Microbial inactivation

    Power ultrasound high power low frequency (20-100kHz)

    Causes cavitation inactivates microbes

    Hot zones kill some bacteria, but they are localized

    Used in conjunction with pressure (manosonication), heat

    (thermosonication), or both (manothermosonication)

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    Microbial inactivation

    More effective when used incombination with otherdecontamination techniques, such asextremes of pH or chlorination

    Patents exist on ultrasonic bacterialinactivation systems

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    Microbial inactivation studies

    Ultrasound not only increases thesusceptibility of microorganisms to heattreatment, but also increased the rate atwhich solid food particles heat.

    Research done on Listeria monocytogenes,strains ofSalmonellaspp., Escherichia coli,

    Staphylococcus aureus, Bacillus subtilisandsome other microorganisms Mamothermosonication increases the lethality

    of heat treatment by @10-fold againstBacillus subtilis(Knorr et.al.,2002).

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    Factors affecting microbial

    inactivation (USDA,2000)

    Amplitude of ultrasonic waves

    Exposure/contact time

    Volume of food being processed

    Composition of the food

    Treatment temperature

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    Advantages of ultrasound overheat pasteurization

    Minimized flavor loss, especially insweet juices

    Greater homogeneity

    Significant energy savings

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    Specific advantages

    Measurements are rapid,nondestructive, precise, fully automated

    Suitable for online measurements

    Can be used to analyze optically opaqueand delicate samples

    No need for extensive samplepreparation

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    Food industries of interest

    Bakery and snack foods

    Candy and confectionery

    Cheese

    Fish

    Prepared meatsVegetable

    Health bars

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    Application of Ultrasound

    Technology in Foods

    Summary

    Ultrasound principle

    Ultrasound applications

    Microbial inactivation

    Applications

    V.B. Alvarez/Ultrasound/10-2007

    References

    http://www.hielscher.com/ultrasonics/cut.htm

    http://www.campden.co.uk/whatsnew/NEWS21.HTM

    Knorr,D, B.I.O.Ade-Omowaye and V.Heinz. 2002. Nutritional improvement of plantfoods by non-thermal processing. Proceedings of the Nutrition Society. 61, 311-318.

    Knorr,D, M.Zenker, V.Heinz and D.Lee. 2004. Applications and potential of ultrasonicsin food processing. Trends in Food Science & Technology. 15, 261-266.

    McClements, D.J. 1995. Advances in the application of ultrasound in food analysis andprocessing. Trends in Food Science & Technology. 6, 293-299.

    Sala,F.J., J.Burgos, S.Condon, P.Lopez and J.Raso. 1995. Effect of heat and ultrasoundon microorganisms and enzymes. In New Methods of Food Preservation, pp.177-203[GW Gould Editor]. Blackie Academic and Professional Publisher, London.

    USDA, 2000. U.S.Food and Drug Administration Report. Kinetics of MicrobialInactivation for Alternative Food Processing Technologies: Ultrasound. Published June2,2000. Available at http://www.vm.cfsan.fda.gov/~comm/ift-us.html

    Withers, P.M. 1996. Ultrasonic, acoustic and optical techniques for the non-invasivedetection of fouling in food processing equipment. Trends in Food Science &Technology. 7, 293-298.