4. Lisbeth Olsson Biochemical conversion 2017-03-28 · 2017. 4. 12. · [email protected]...
Transcript of 4. Lisbeth Olsson Biochemical conversion 2017-03-28 · 2017. 4. 12. · [email protected]...
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INDUSTRIAL BIOTECHNOLOGY
Lisbeth OlssonIndustrial biotechnology, Chalmers
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INDUSTRIAL BIOTECHNOLOGY
Industrial Biotechnology Department of Biology and Biological Engineering
Chalmers University of Technology Göteborg, Sweden
Bioethanol and bioproducts for the bio‐based economy – development of robust
cell factories and robust processes
Maurizio Bettiga, Carl Johan Franzén, Johan Larsbrink, Lisbeth Olsson
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INDUSTRIAL BIOTECHNOLOGY
Enzymatichydrolysis
Product
Fermentation
Enzymediscovery and design
Propagation Cell factory
Fermentable sugar stream
Raw material stream containing mono‐, oligo and polysaccharides, lignin, inhibitors (furans, weak acids and phenolics)
Process integration and optimization
Biochemical conversion of biomass
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INDUSTRIAL BIOTECHNOLOGY
Robust microorganisms and processes ?• Microbial processes receive more and more industrial importance in line with bio‐based economy solutions
• Understanding and ability to improve robustness increase the industrial potential for microbial conversion
• Developing robust process allow for maximum utilization of the potential of the production host
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Robustness, the ability to maintain performance in the face of perturbations and uncertainty, is a long‐recognized key
property of living systemsStelling J et al. (2004) Cell 118:675‐685
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INDUSTRIAL BIOTECHNOLOGY
Enzymes
• Biological catalysts• Act on specific chemical bonds
• Has a certain size
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Process robustness
Koppram, R., E. Tomas‐Pejo, Elia, C. Xiros, L. Olsson (2014) Trends in Biotechnology, 32, 46‐53
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INDUSTRIAL BIOTECHNOLOGY
Multi‐feed SSCF – Process design
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• Fed‐batch SSCF with feeds of solids, yeast, (and enzymes)
• Integrated yeast propagation and adaptation
Koppram and Olsson, Biotechnol Biofuels 2014, 7:54, Wang et al., Bioresource Technol 2014, 172:303‐311Wang et al Biotechnol Biofuels 2016, 9:88
SSCF
Liquidfraction
Cell Feed
SolidsFeed
Continuous yeast cultivation
Pretreated wheat straw
Enzymes
Strategies for more robust processes
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Ethanol yield: 56%Ethanol yield: 13%
20 % WIS SpruceBatch SSF Multi‐feed SSF
Koppram and Olsson, Biotechnol Biofuels 2014, 7:54
Multi‐feed SSF improves mixing and reduces inhibition of hydrolysis and fermentation
Strategies for more robust processes
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INDUSTRIAL BIOTECHNOLOGY
30
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Max
imal
EtO
H ti
ter
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> 20% WIS
Model‐based feed of solids
Flocculating cells
New material, more hydrolysate in propagation, temperature decrease
Quicker feed of solids, more hydrolysate in SSF (strain 1 and 2)
15% WIS A
B
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D
E
F
Initial enzyme, cell feed
Enzyme feed, Initial cell
Multifeed SSF allowsfor improved highgravityfermentations – highcell viability is a key
Strategies for more robust processes
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INDUSTRIAL BIOTECHNOLOGY
From sugar to polymers using cell factories
Microbial conversion –development of microbial catalyst
Sugar rich biorefinerystreams
Polymeric building Polymeric building blocks; 3‐hydroxy propionic acid, lactic aid, adipicacids
Using strain engineering we develop the microorganism to:• Produce the targetted product at high yield and productivity• Being robust to industrially prevailing conditions
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INDUSTRIAL BIOTECHNOLOGY
11Inhibitors influence cellular functions in numerous ways
Cellular robustness
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Reduce uptakeEnhance excretion
Reduce damage
Enhance repair
Enhance conversion (detoxification)
Strain improvement strategies for increased cellular robustness should enable functions including
Cellular robustness
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INDUSTRIAL BIOTECHNOLOGY
0
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0 5 10 15 20 25 30
8 μmol glutathione/(g DCW)
Concen
tration (g/l)
Time (h)
ETHANOL‐CONTROL STRAIN
ETHANOL‐GSH STRAIN
Ask et al. (2013) Microbial Cell Factories 12:8713
Fermentation performance of glutathione modulated strains
12 μmol glutathione/(g DCW)
Cellular robustnessReduce damage and increase repair
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INDUSTRIAL BIOTECHNOLOGY
Robust processes and cellular robustness in a biorefinery perspective
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Thanks to ….Industrial Biotechnology group members
Our research within cellular robustness process robustness was funded by
… and collaborators