3rd “PARA” AO -DAY - unipr.it · 1990-1991 Chair of Genetics at the University of Bologna ......
Transcript of 3rd “PARA” AO -DAY - unipr.it · 1990-1991 Chair of Genetics at the University of Bologna ......
3rd “PARMA” NANO-DAY The event that brings together students, researchers, enterprises
CENTRO CONGRESSI, AULE DELLE SCIENZE
CAMPUS DELLE SCIENZE E DELLE TECNOLOGIE, UNIVERSITÀ DI PARMA
JULY 12-14, 2017
PROGRAM & BOOK OF ABSTRACTS
3rd “PARMA” NANO-DAY
The event that brings together students, researchers, enterprises
JULY 12-14, 2017
CENTRO CONGRESSI, AULE DELLE SCIENZE
CAMPUS DELLE SCIENZE E DELLE TECNOLOGIE UNIVERSITÀ DI PARMA
ORGANIZED BY
Prof. Nelson Marmiroli - Dept. Chemistry, Life Sciences and Environmental Sustainability, UniPR
Director CINSA
Dr. Salvatore Iannotta - Director IMEM-CNR, Parma
http://www2.bioscienze.unipr.it/nano-day/ @ParmaNanoDay
Published by Università di Parma
Edited by Nelson Marmiroli and Salvatore Iannotta
© 2017 by Nelson Marmiroli and Salvatore Iannotta
(Authors of the single lecture/poster abstracts here published
are jointly-responsible for their correspondent submitted content)
ISBN 978-88-941066-8-8
3rd “PARMA” NANO-DAY, July 12-14, 2017
4 Preface
The conference, now at its third edition, represents an important meeting point for anybody
working on nanotechnology, not only in the academic world, but also in the industrial one.
The object is to organize an event focussed on young researchers, and their work. To promote
broad participation, the 3rd
Parma Nano-Day is completely free to all participants.
The event will take place from the 12th
to the 14th
of July. In the morning of July 12th
, a round
table will be organized with title “Technologies and Sustainable Development”. At the
discussion will attend important figures from different institutions, the academic world, and
the industry. In the afternoon of the same day, there will be a job day where industries, and
academic spin-offs will introduce their new line of research in the nanotechnology field. This
event will be followed by a session where several funded projects will be briefly presented.
The third part will focus on scientific talks (13th
and 14th
of July) divided in four sessions:
Technologies and Applications; Regulation and Economics; Agri-food, Environment and
Biotechnologies; and last Medicine and Health. The program will see almost 30 speakers
alternating the stage. Among those, it is worth mentioning: Dr. Ciro Chiappini (King’s
College London, UK), Dr. Soren Bowadt (European Commision), Dr. Jason C. White (CAES,
USA), and Dr. Isabella De Angelis (Istituto Superiore di Sanità). Moreover, are expected
almost 50 poster presentations, and the best ones will be awarded in the afternoon of the last
day.
3rd “PARMA” NANO-DAY, July 12-14, 2017
5 Organizers
THE ORGANIZERS
Prof. Nelson Marmiroli
Dept. Chemistry, Life Sciences and Environmental Sustainability, Parma
tel. 0521905606
Education and professional experience
1967-1971 degree in Biological Sciences at the University of Parma
1973-1977 Research Assistant at the University of Parma
1978 Associate Professor at the University of Chicago (IL, USA)
1979-1982 Adjunct Professor of Agricultural Genetics at the University of Udine
1980-1986 Associated Professor in Applied Genetics at the University of Parma
1986-1990 Full Professor of Genetics (Chair) at the University of Lecce
1990-1991 Chair of Genetics at the University of Bologna
1991-1995 Chair of Biology at the University of Parma
Since 1995 Chair of Recombinant DNA Technologies at the University of Parma
Current titles and assignements
Rector Delegate and President of the Committee for University Sport Activities; Coordinator of the
Unit "Biotechnology, Ecology, Sustainability" of the Department; Member of the Coordinators'
Committee of the Department; Director of the CINSA (National Interuniversity Consortium for
Environmental Sciences).
He has authored over 300 scientific publications. Milton P. Gordon Award for excellence in the career
related to environmental biotechnologies (International Phytotechnology Society, 2013).
Main research activities
- Application of environmental biotechnologies for sustainability. Phytoremediation, bioremediation,
emerging contaminants (nanomaterials and nanoparticles), interaction of plants with pollutants
(outdoor and indoor).
- Genomics and proteomics of the response to environmental stress; genes and gene products involved
in the adaptive response and in response to stress; new proteomic tools based on 2D analysis and mass
spectrometry.
- Analysis of the effects of environmental stress and agricultural conditions on protein composition of
cerela grains (durum wheat and rice), through proteomic, ionomic and ultrastructural analyses.
Analysis of the effects of nanoparticles of different structure on plant, fungal and animal organisms
subjected to environmental stress.
- Development of molecular biosensors and nanosensors based on protein and DNA for analysis of
food composition and quality; development of microsensors for on-line monitoring of food supply
chains, development of Lab-on-chip systems. Molecular traceability in food and feed.
- Development of systems for analysis of gene expression regulation mediated by microRNAs,
concerning occurrence of infective immunological and inflammatory diseases in man.
- Development of innovative tools for toxicological analyses.
- Innovative methodologies as countermeasure against deliberate threats towards environmental and
food resources.
- Production of selected plant species which can be used as natural resources to decrease the impact of
conventional energy sources, using renewable resources in the production of biofuels.
3rd “PARMA” NANO-DAY, July 12-14, 2017
6 Organizers
Dr. Salvatore Iannotta
IMEM-CNR, Parma
tel 0521269225
Major research interests
Synthesis and studies of nanostructured and molecular materials; organic electronics; bioelectronics;
memristive devices and systems and nanomedicine. Active and passive gas- and bio-sensors, trace-gas
detection and related applications.
Education
1976 – Degree in Physics at the University of Bologna
1984 – PhD. in Chemistry at the (GWC) (Guelph Waterloo Centre for Graduate Work in Chemistry-
Waterloo - Ontario Canada)
Current position
Director of CNR Istituto dei Materiali per l'Elettronica ed il Magnetismo (IMEM) and of the
BIOGENA-P Center, BIOelectronics GEnomics and NAnomedicine of CNR and University of Parma.
He is Professor at the PhD School on Science and Technology of Materials of the University of Parma
(since 2009).
Professionals and intramural responsibilities
He has been science visitor at several research institutes among which the Nanoscale Facility of
Cornell University and has been visiting scientist at the Lash Miller Laboratory of the University of
Toronto (Ontario–Canada). He has been member of the board of directors of the Bruno Kessler
Foundation (FBK) and of scientific/stirring committees of national and International conferences
(among some recent ones: ECOSS-26 –European Conference on Solid Surface August 2009, CIMTEC
2012; MAMA –Hybrids October 2012; chair 20th International Symposium on Metastable,
Amorphous and Nanostructured Materials - ISMANAM 2013, chair ICNAAM 2014 Symposium on
"Adaptive Materials, Devices and Systems Towards Unconventional Computing and Robotics:
Modeling and Implementation", chair of E-MRS 2016 Symposium on "Adaptive Materials, Devices
and Systems"). He is coordinator of several research projects on a national and international basis in
the fields of nanostructured, molecular, hybrid materials and systems including their applications to
gas and bio-sensing, energy (photovoltaics in particular). He is co-author of more than 150 papers on
journals with international editorial boards with more than 3100 citations and he has been invited
speaker to more than 50 international conferences. He is also author or co-author of several
monographs, among which: "Cluster Beam Synthesis of Nano-structured Materials" – Springer –
Berlin (http://link.springer.com/book/10.1007/978-3-642-59899-9). He is promoter of several national,
European and international scientific collaborations/initiatives and member of the International Union
for Vacuum Science Techniques and Applications (IUVSTA) - Nanostructures Division Committee.
He is also member of the board of directors of the Ph. D. Schools: Science and Technology of the
University of Parma. Since 2014 he is President of the Panel Energy, Micro- and Nanoelectronics for
the evaluation of proposal whitin the MISE-FCS Programme (the Italian Ministry of Economical
Developments Programme for Competitive Growth). He has been acting as research proposal reviewer
for several international agencies.
3rd “PARMA” NANO-DAY, July 12-14, 2017
7 Steering Committee
STEERING COMMITTEE
Prof. Elena Maestri Dept. SCVSA, UniPR
Prof. Stefano Selleri Dept. Engineering and Architecture, UniPR
Prof. Marina Caldara Dept. SCVSA, UniPR
Prof. Marta Marmiroli Dept. SCVSA, UniPR
Prof. Roberta Ruotolo Dept. SCVSA, UniPR
Dr. Michela Janni IMEM-CNR, Parma
Dr. Caterina Agrimonti Dept. SCVSA, UniPR
Dr. Alessia Comastri Dept. SCVSA, UniPR
Dr. Sara Graziano Dept. SCVSA, UniPR
Dr. Davide Imperiale Dept. SCVSA, UniPR
Dr. Giacomo Lencioni Dept. SCVSA, UniPR
Dr. Francesca Mussi Dept. SCVSA, UniPR
Dr. Laura Paesano Dept. SCVSA, UniPR
Dr. Luca Pagano Dept. SCVSA, UniPR
Dr. Graziella Pira Dept. SCVSA, UniPR
With the kind collaboration of
Dr. Marina Cassano U.O. Ricerca Competitiva, UniPR
Dr. Mimmo Cavalca Dept. SCVSA, UniPR
Dr. Doretta Fava U.O. Placement e Rapporti con le Imprese, UniPR
Dr. Licia Gambarelli U.O. Ricerca Competitiva, UniPR
Dr. Guido Giombi C.I.R.E.A., UniPR
Dr. Vanni Villa U.O. Approvvigionamenti, UniPR
3rd “PARMA” NANO-DAY, July 12-14, 2017
10 Scientific Committee
Prof. Andrea Caneschi
INSTM Director, UniFI
Prof. Maria Careri
Dept. SCVSA, UniPR
Prof. Paolo Cescon
IDPA-CNR, Venice
Prof. Attilio Corradi
Dept. Veterinary Science, UniPR
Prof. Antonio D’Aloia
Director of the Centre of Bioetics, UniPR
Prof. Roberto De Renzi
Dept. Mathematical, Physical and Computer Sciences, UniPR
Prof. Luca Di Nella
Dept. Economics and Management, UniPR
Prof. Giorgio Dieci
Dept. SCVSA, UniPR
Prof. Giovanni Franceschini
Vice Rector, Dept. Engineering and Architecture, UniPR
Prof. Rinaldo Garziera
Dept. Engineering and Architecture, UniPR
Prof. Alessandro Mangia
Dept. SCVSA, UniPR
Prof. Antonio Mutti
Dept. Medicine and Surgery, UniPR
Prof. Ludovico Valli
Dept. Biological and Environmental Science and Technology, UniSalento
3rd “PARMA” NANO-DAY, July 12-14, 2017
12 Round Table
Simona Caselli
Assessore all'agricoltura, caccia e pesca Regione Emilia Romagna, Bologna, IT
E’ nata a Parma nel 1961. Laureata in Economia e Commercio all’Università di Parma, è revisore
contabile iscritta all’Albo. Dirigente di Legacoop nazionale e regionale, è stata presidente di Legacoop
Emilia Ovest (che raggruppa le province di Reggio Emilia, Parma e Piacenza), dopo aver guidato per
quattro anni la Legacoop di Reggio Emilia. E’ stata anche Direttore Commerciale e Sviluppo del
CCFS, il Consorzio finanziario nazionale di Legacoop con sede a Reggio Emilia. Ha ricoperto svariati
incarichi come consigliere di amministrazione e di sindaco in società cooperative. In particolare è stata
amministratore di Coopfond, il Fondo Nazionale di promozione cooperativa di Legacoop e di Quadir
spa, società di alta formazione cooperativa; consigliera di amministrazione di Coop Consumatori
Nordest e membro della Direzione Operativa dell’Associazione Nazionale delle Cooperative Agricole
per le politiche finanziarie. Ha collaborato a diverse pubblicazioni su temi economico-finanziari e
sulla cooperazione ed ha svolto attività di docenza in Master in Economia Cooperativa. E’ stata
consigliere di amministrazione dell’Università di Parma.
Palma Costi
Assessore alle attività produttive, Regione Emilia Romagna, Bologna, IT
E’ nata a Camposanto, in provincia di Modena, nel 1957. E’ stata presidente dell’Assemblea
legislativa della Regione Emilia-Romagna nella precedente legislatura. Laureata in Storia
contemporanea, ha ricoperto diversi incarichi tra cui quello di sindaco di Camposanto (1982-1991). E’
stata responsabile della Segreteria dell’assessore regionale alla Sanità, Giuliano Barbolini; dal 1994 al
1995 responsabile dei Servizi sociali del Comune di Mirandola e dal 2004 al 2006 dell’Ufficio
Relazioni con il Pubblico/Servizi Demografici, sempre del Comune di Mirandola. Ha ricoperto il ruolo
di assessore alla Pianificazione del Comune di Modena - dal 1995 al 2004 - e di assessore alle Attività
produttive, pari opportunità, risorse umane e semplificazione della Provincia di Modena dal 2006 al
2010.
Giovanni Franceschini
Dip. di Ingegneria e Architettura, UniPR, Parma, IT
Giovanni Franceschini si è Laureato in Ingegneria Elettronica presso l'Università di Bologna. Dal 1987
al 1989 svolge attività di ricerca presso il CESI (Centro Elettrotecnico Italiano) di Milano. Nel 1990 è
ricercatore presso l'Università degli Studi di Parma dove, attualmente, e' Professore Ordinario del
raggruppamento ING-IND/32 "Convertitori, Macchine e Azionamenti Elettrici".
Da gennaio 2011 a novembre 2013 è stato Direttore del Dipartimento di Ingegneria dell’Informazione,
Università di Parma. Da novembre 2013 ricopre l’incarico di prorettore vicario dell’Università di
Parma con delega ai sistemi informativi. L'attivita' di ricerca del Prof. Franceschini si colloca nel
campo della modellistica, progettazione e diagnostica degli azionamenti elettrici ad alta dinamica e sui
sistemi di conversione statica dell'energia. E' revisore per le Transaction on Industrial Electronics e
Industry Applications. E' autore o coautore di oltre 150 lavori pubblicati a convegni o su riviste
internazionali.
3rd “PARMA” NANO-DAY, July 12-14, 2017
13 Round Table
Flavia Barone Istituto Superiore di Sanità, Roma, IT
Laureata in Scienze Biologiche presso l’Università La Sapienza di Roma è primo ricercatore presso il
Dipartimento Ambiente e Salute dell’Istituto Superiore di Sanità, dove svolge la propria attività
istituzionale e di ricerca. E’ membro designato al Working Party on Manufactured Nanomaterials
dell’OECD, esperto designato nel GdL ISS “Nanomateriali e salute” e nel GdL Nazionale ISPESL sui
nanomateriali, esperto nel CtC REACH del Ministero della Salute GdL Nanomateriali. Principali
progetti nazionali e internazionali: coordinatore per l’ISS del progetto FP7 NANoREG-A common
European approach to the regulatory testing of Manufactured Nanomaterials (2013-2017);
convenzione ISS - Regione Lazio “RInnovaReNano - Ricerca e innovazione responsabile delle
nanotecnologie” (2015-2017); progetto H2020 NanoReg2-Development and implementation of
Grouping and Safe-by-Design approaches within regulatory frameworks (2015-2018); referente per
l’ISS (Additional Partner) del progetto H2020 EC4SafeNano - European Centre for Risk Management
and Safe Innovation in Nanomaterials & Nanotechnologies (2016-2019).
Alberto Manzo
MIPAAF, Roma, IT
Il Dr. Alberto MANZO è laureato in Scienze Agrarie, presso l’Università degli Studi di Napoli, Portici
e ha conseguito la specializzazione post-laurea in Biotecnologie Vegetali presso la Scuola di
Specializzazione dell'Università degli Studi di Pisa, ed ha conseguito presso l’Università di Perugia il
titolo di Dottore di Ricerca in Biotecnologie agro ambientali.
Dal 1988, è entrato nei ruoli del Ministero partecipando a numerose commissioni Nazionali ed
Internazionali. E’ stato esperto nazionale designato presso la Commissione UE nel Gruppo “COEX-
NET” nonché membro della delegazione italiana nel Comitato biologico (SCOF). Dal 2006 al 2009 è
stato dirigente dell’Ufficio “Agricoltura biologica” del Ministero delle politiche agricole alimentari e
forestali. Dal 2009 al 2012 ha ricoperto l’incarico di dirigente dell’Ufficio “Agroenergie e filiere
agricole e agroalimentari minori” del Ministero delle politiche agricole alimentari e forestali ordinando
il Tavolo Bioenergie.
Andrea Pontremoli
Dallara S.p.A, Parma, IT
Andrea Pontremoli entra in IBM nel 1980, assunto come semplice tecnico di manutenzione percorre la
carriera professionale fino ad essere nominato nel 2004 Presidente e Amministratore Delegato IBM
Italia. Nell’ottobre del 2007 lascia l’incarico di Presidente e Amministratore Delegato di IBM Italia e
Amministratore Delegato e Direttore Generale di Dallara Automobili. Da novembre 2007, è Direttore
del “Executive Master in Technology and Innovation Management” presso ALMA Graduate School,
Università di Bologna. Da aprile 2008, è nel CdA e nel Comitato Esecutivo di Barilla S.p.A. Da
maggio 2012, è Lead Indipendent Director nel CdA della Brunello Cucinelli S.p.A. Nel 2004
l’Università di Parma gli conferisce la laurea Honoris Causa in Ingegneria Informatica. Da tempo
Pontremoli fa parte delle Giunte di Confindustria, Assolombarda ed Assonime. A ciò si aggiunge
l’appartenenza al direttivo del “Council of Relationship Italy and USA” ed è membro dell’Aspen
Institute Italia.
3rd “PARMA” NANO-DAY, July 12-14, 2017
14 Round Table
Paolo Cescon
IDPA-CNR, Venezia, IT
Prof. Ordinario di Chimica Analitica all’Università Ca’ Foscari Venezia, già Preside della Facoltà di
Scienze e Pro Rettore Vicario. Incarichi istituzionali e professionali: Membro Comitato Scientifico
dell’Istituto Superiore di Sanità, Direttore Istituto Dinamica dei Processi Ambientali- CNR,
Commissario Straordinario Centro Nazionale di Ricerche in Agricoltura (CRA), Membro
Commissione Scientifica Nazionale del Programma di Ricerche in Antartide (PNRA), Membro
Ufficio di Piano (PCM) per il monitoraggio di piani riguardanti la protezione di Venezia, Membro
Commissione Nanotech della Regione del Veneto. E’ autore, su riviste scientifiche internazionali, di
numerose pubblicazioni dedicate allo sviluppo e applicazione di metodologie analitiche in settori come
la caratterizzazione di elementi in matrici reali, lo studio di processi naturali che influenzano il clima.
RICONOSCIMENTI: Medaglia Lavoisier, Euroanalysis VI 1987 - Premio Nazionale INARC 1989 –
Premio della Terra 2002 – Medaglia Canneri, Società Chimica Italiana 2005.
Lanfranco Masotti Consorzio Italbiotec, Milano, IT
Presidente del Consorzio Italbiotec e già Presidente del corso di laurea specialistica in Biotecnologie
Farmaceutiche dell’Università Alma Mater Studiorum di Bologna. Laureato in Chimica Industriale è
stato inoltre coordinatore del Dottorato di ricerca in Biotecnologie Cellulari e Molecolari e direttore
del Dipartimento di Biochimica “G. Moruzzi” di detta Università. È autore di oltre 160 pubblicazioni
scientifiche su riviste nazionali ed internazionali. Alla presidenza del Consorzio, un ente pubblico-
privato no-profit con quasi 20 anni di esperienza, si occupa della promozione delle biotecnologie,
grazie alla collaborazione tra Università, centri di ricerca ed industrie. Il Consorzio è Regional Branch
Office per la Regione Italia dell'European Federation of Biotechnology (EFB), membro del Cluster
Tecnologico Nazionale SPRING e coordinatore del Cluster Regionale Lombardo della Chimica Verde
(LGCA). In questo contesto si impegna per lo sviluppo della bioeconomia, un passaggio obbligato per
affrontare le grandi sfide planetarie: mutamenti climatici, scarsità di risorse, tutela dell’ambiente,
multifunzionalità dell’agricoltura, salute, miglioramento delle condizioni di vita della popolazione,
nuove opportunità di crescita e occupazione, rafforzamento della competitività delle imprese.
Francesco Ausiello ASTER Emilia Romagna, Bologna, IT
Laureato in Ing. Meccanica indirizzo meccatronico diviene ingegnere in Fiat Direzione Ricerca
applicata nell’ambito di studi generali sul sistema energetico Italia e sul veicolo in rapporto al
risparmio energetico. Assunto in Magneti Marelli PWT è direttore del gruppo che ha ideato e
sviluppato il 1° sistema Common Rail per l’iniezione elettronica diesel. Direttore R&S MM Divisione
motori ed alternatori elettrici sviluppa l’ultima generazione di Alternatori e Starter, e in tale ruolo
viene assunto dalla DENSO ltd con la stessa gamma prodotto. Rientrato in Marelli fonda il gruppo J
RAUM (Joint Research Area University Marelli). Conclude la carriera in MMarelli Holding come
direttore della Innovazione Corporate. Dal 2009 Assume la carica di Direttore Tecnico ASTER per la
promozione della rete di alta tecnologia dell’Emilia Romagna; attualmente ricopre la carica di
responsabile dei progetti strategici di ASTER, e opera come rappresentante regionale nel Progetto
Vanguard Initiative.
3rd “PARMA” NANO-DAY, July 12-14, 2017
15 Round Table
Antonio D’Aloia
Centro Bioetica, UniPR, Parma, IT
Antonio D’Aloia, è Professore Ordinario di Diritto Costituzionale nell’Università di Parma, dove
insegna anche Biodiritto. Ha fondato, e ne è Direttore scientifico, University Center for Bioethics,
Centro Interdipartimentale di studi bioetici dell’Ateneo parmense; è vice-Direttore della Rivista on
line Biolaw Journal. Dal 2015, è membro del Consiglio Direttivo dell’Associazione Italiana dei
Costituzionalisti.
Da anni si occupa delle connessioni tra diritto e sviluppi della scienza e della tecnica. Ha scritto e
curato decine di pubblicazioni sui principali temi della bioetica e del biodiritto: decisioni di fine vita,
procreazione medicalmente assistita, wrongful birth e wrongful life, responsabilità medica, protezione
dei dati genetici, ruolo del giudice nei conflitti biogiuridici, enhancement, bioetica ambientale e tutela
delle generazioni future, diritto e climate change, decisione legislativa e razionalità scientifica.
3rd “PARMA” NANO-DAY, July 12-14, 2017
18 Chairmen
Ordered following the programme
Prof. Enrico Dalcanale Scientific Director Functional Materials section, INSTM, Florence, IT
Enrico Dalcanale graduated in Industrial Chemistry (cum laude) at the University of Bologna in 1981.
After working as research scientist at the Donegani Research Institute of Montedison in Novara from
1982 to 1990, he joined the Faculty of the Department of Chemistry of the University of Parma, where
he is currently Full Professor in Industrial Chemistry. In 1985-86 he spent a sabbatical year in the
group of D. J. Cram (Nobel Laureate 1987) at UCLA. In 2004 he has been visiting professor at Naval
Research Laboratory (Washington DC, USA). He is the founder of SOATEC, a University spin-off
company active in the field of environmental and food sensors. He is presently the Scientific Director
of the Functional Materials section of the Italian Consortium for Materials Science (INSTM). He has
published over 160 research papers, 16 reviews and he holds 15 patents. He presented the results of his
research in over 110 research lectures in conferences and invited seminars worldwide. The research
profile of ED's group is defined by the supramolecular approach to materials science, giving a
privileged position to molecular recognition and self-assembly as operating tools. The major fields of
activity have been responsive surfaces, supramolecular polymers and sensors.
Prof. Roberto De Renzi Dept. Mathematical, Physics and Computer Sciences, UniPR, Parma, IT
Prof. Roberto de Renzi born in Pavia in 1955. In 1979 he got an honours degree, the University of
Parma. In 2002 he became full professor in Physics at University of Parma. He coordinated the Parma
Research on Magnetism (PaRMa) Laboratory and reorganized the solid state NMR laboratory of the
Department of Physics in Parma. He developed muon spin spectroscopy: from the early exploratory
experiments at CERN (Geneve, 1979-1987), and designed and commissioning of the pulsed source at
ISIS (Rutherford Laboratory, 1985-1989). He had a scientific advisory role at ISIS, at the Paul
Scherrer Institute (Villigen, CH) and at J-PARC (Tokai, JP). In 2017 he became Head of the
Department of Mathematical, Physics and Computer Sciences, Parma.
Publications and Distinctions: Author of 187 papers, coauthor of a condensed matter textbook.
Yamazaki Prize 2014 recipient from the International Society for Muon Spin Rotation APS
Outstanding Referee 2013.
Prof. Rinaldo Garziera Dept. Engineering and Architecture, UniPR, Parma, IT
Professor. Rinaldo Garziera, born in Como in 1962, began his scientific career at the Normale Scuola
di Pisa in 1980. From 1985 to 1986 he worked as a temporary researcher at Ispra's Euratom Center. He
graduated from the Politecnico di Milano in Mechanical Engineering in 1987, where he has been a
Ph.D. student since 1989. In 1991 became a Researcher in the Industrial Engineering Department at
the University of Parma. He became associate professor in the same university in 1998, and received
the Applied Mechanics Chair at the Machines in January 2001. In his research activity he was
involved with automatic machines, vibrations of fluid interacting structures, nonlinear structures and
vibration monitoring. He has held the Master of Mechanical Engineering Fundamentals course, and
for some years has been director of the Industrial Engineering Department of the University of Parma.
3rd “PARMA” NANO-DAY, July 12-14, 2017
19 Chairmen
Prof. Maria Careri
Dept. Chemistry, Life Sciences and Environmental Sustainability, UniPR, Parma, IT
Full Professor of Analytical Chemistry at Parma University since 2001. Head of the
Chemistry Department of Parma University since July 2012 up to December 2016. Board Member of
University of Parma (Term 2017-2020).Director of the University Master Course "Laboratory Quality
Systems" from 2001-2002 to 2010-2011 academic years. Chair elected of the Executive Board of the
Division of Analytical Chemistry of the Italian Chemical Society (2007 – 2009). Director of the
National School of "Analytical and Bioanalytical Methodologies based on Mass Spectrometry" of the
Italian Chemical Society, which is hold in Parma starting from 2005. Member of the International
Advisory Board of Analytical and Bioanalytical Chemistry (Springer) since January 2008.
Representative of the University of Parma in the board of JRU “METROFOOD-IT”. Author of more
than 170 scientific papers on international peer-reviewed journals and 2 patents. Invited speaker at
national and international symposia.
Dr. Andrea Zappettini IMEM-CNR, Parma, IT
Andrea Zappettini is senior researcher at IMEM-CNR and Head of the SIGNAL research group, whose
main activities are: i) the development of CdZnTe-based gamma ray detector ii) the realization and
characterization of metal oxide nanostructures for piezo and gas sensing iii) the integration of organic
electrochemical transistors into textile and into plants.
He is author of over 180 scientific publications in international journals (source: Web of Science), that
have cited more than 2000 times, his h-index is 25, and he is author of 10 international patents and 4
Italian patents.
Since 2008 he has been professor at the PhD course in Materials Science at the University of Parma.
Since 2011, he has been Chair of the Commission for Crystal Growth and Characterization of
Materials of the International Union of Crystallography and ex-officio member of the Executive
Committee of the International Organization for Crystal Growth. Since 2015 he is Coordinator of the
Crystal Growth Section of the Italian Crystallography Association.
Prof. Luca Di Nella Dept. of Economics and Management, UniPR, Parma, IT
Degree in Law with full marks cum laude. Postgraduate Specialization in civil law at School of Civil
law at University of Camerino with full marks cum laude. Research doctorate in "Civil Law in
constitutional legality". Research scientist - full post in research sector IUS/01 Private Law, UniPR
(2000) Assistant professor in research sector IUS/01 Private Law, UniPR (2002) Full professor in
research sector IUS/01 Private Law, UniPR (2006) Member of supervision team on research doctorate
programme "The individual's problems in civil law" based at University of Sannio, and member of
supervision team on research doctorate programme in "Civil Law in Constitutional Legality"
University of Camerino, from fifteenth course. Since 2009 is Responsible of the University of Parma
for the international Master Strategia e Pianificazione delle Organizzazioni, degli Eventi e degli
Impianti sportivi. Since 2010 is Coordinator of Law Section and member of Scientific Board of the
review Associazioni e sport. Since 2011 is Director of the Centro di Ricerche sullo Sport (CeRS,
UniPR). Since 2009 is member of Tribunale Nazionale di Arbitrato per lo Sport of CONI.
3rd “PARMA” NANO-DAY, July 12-14, 2017
20 Chairmen
Dr. Søren Bøwadt European Commision, DG Research & Innovation, Brussels, BE
Dr. Søren Bøwadt has been working for the European Commission's DG Research and Innovation
since 1999 and is currently the Deputy Head of Unit for the "Advanced Materials and
Nanotechnology" Programme. His main educational background in organic synthetic and analytical
chemistry has been obtained from the University of Odense, Denmark. Søren Bøwadt has been
responsible for over 150 scientific projects in various areas of natural science, setting up the SPIRE
public private partnership and has published more than 50 peer-reviewed papers in various areas of
chemistry related research.
Prof. Ruggero Bettini Pharmaceutical Technology, Food and Drug Department, UniPR, Parma, IT
Ruggero Bettini in 1994 got his Ph.D. in Pharmaceutical Chemistry and Technology, University of
Pavia. Currently is Professor of Pharmaceutical Technology, Food and Drug Department, University
of Parma, Italy. Is Director of the Interdepartmental Centre for Innovation in Health Products,
Biopharmanet-TEC, University of Parma, Italy. He is also Director of the Master course in
“Pharmaceutical Technology and Regulatory Affairs”, University of Parma. His research activity is
mainly focused on Solid dosage forms for controlled and site specific drug delivery, and solid-state
chemistry for improving biopharmaceutical properties of active pharmaceutical ingredients; swelling
controlled systems for oral drug delivery; nasal and pulmonary administration of powders;
pharmaceutical applications of supercritical fluid technologies; solid-state chemistry of drugs; natural
polymers for regenerative medicine applications.
Prof. Giorgio Dieci Director Dept. Chemistry, Life Sciences and Environmental Sustainability, UniPR, Parma, IT
Giorgio Dieci, Professor of Biochemistry at the University of Parma, studies the molecular
mechanisms of gene transcription and its regulation, with recent emphasis on human retrotransposon
silencing control. After the Ph.D. in Molecular Biology and Pathology, he was EMBO post-doctoral
fellow at CEA-Saclay (Gif-sur-Yvette, France) where he contributed to clarify fundamental aspects of
the transcription process. He later joined the University of Parma, where he coordinated several
national scientific programs and an international team supported by the Human Frontier Science
Program. Studies carried out within these programs gave rise to many collaborations with research
groups in USA, France, Japan, Switzerland and Italy, and led to scientific achievements including a
better understanding of the RNA polymerase III transcription system and the discovery of novel
regulators of ribosome biogenesis in eukaryotic cells. He is presently supported by a grant from the
Italian Association for Cancer Research (AIRC) for the study of the connections between
retrotransposons and cancer. For the period 2017-2019 he has been appointed Head of the new
Department of Chemistry, Life Sciences and Environmental Sustainability of the University of Parma.
3rd “PARMA” NANO-DAY, July 12-14, 2017
21 Chairmen
Prof. Marta Marmiroli Dept. Chemistry, Life Sciences and Environmental Sustainability, UniPR, Parma, IT
BSc (Hons) in Solid State Physics, Parma University. PhD in Biotechnology, Parma University,
“Applications of scanning electron microscopy, microanalysis, microfluorescence and EXAFS to
environmental biotechnology”. OECD grant for research on N use in agriculture. Senior Researcher at
Parma University, scientific section BIO/13. Lecturer in Applied Biotechnology and
Phytoremediation. Research focus on food security in several EU and NATO projects. Collaboration
with Canterbury and Lincoln University, NZ; CAES (The Connecticut Agricultural Experiment
Station, CT., USA); The James Hutton Institute, UK; ELETTRA synchrotron, Trieste. Main interest in
investigation of plant-metals /ENMs interaction through advanced “-omics”, microscopy, EDX, and
synchrotron-light based methods.
Prof. Jason C. White Vice Director Connecticut Agricultural Experiment Station, CT, USA
I am currently Vice Director of the Connecticut Agricultural Experiment Station, as well as Head of
the Department of Analytical Chemistry. I am also State Chemist. I received a B.S. in Ecology from
Juniata College in Huntingdon, PA in 1992. I received my Ph.D. in Environmental Toxicology from
Cornell University in 1997. I did a one-year post-doctoral position at the Connecticut Agricultural
Experiment Station in New Haven CT from 1997-1998 in the Department of Soil and Water. I also
have adjunct status at the University of Texas-El Paso, University of Massachusetts, and Post
University. I am Managing Editor for the International Journal of Phytoremediation, Immediate Past
President of the International Phytotechnology Society, on the Editorial Advisory Board (SAB) of
Environmental Science and Technology and Environmental Science and Technology Letters, and on
the editorial boards of Environmental Pollution and NanoImpact. My primary research interests
include nanotoxicology, nano-enabled agriculture and food safety. I live in Prospect CT USA with my
wife Michelle and 6 children.
Dr. Rita Baraldi IBIMET-CNR, Bologna, IT
Doctor in Agricultural Science, Dr. Rita Baraldi is a Senior Scientist at the Institute of Biometeorology
(IBIMET) of the National Research Council. Since 2009 responsible of the detached branch of
IBIMET-CNR in Bologna, Italy. Her research activities are mainly focused on: Atmosphere/biosphere
exchange, Ecophysiological studies on volatile organic compound emission (VOC) from vegetation at
plant and ecosystem scale, Plant physiology and ecophysiology, Auxin and abscisic acid biosynthesis
and metabolism in association with various aspects of plant, bacteria and fungal development,
phytohormones as intermediates between light signal and morphogenesis. She is author of several
publications in international journals.
3rd “PARMA” NANO-DAY, July 12-14, 2017
22 Chairmen
Prof. Elena Maestri Dept. Chemistry, Life Sciences and Environmental Sustainability, UniPR, Parma, IT
Elena Maestri has a degree in Biology and a PhD in Genetics. She is Full Professor of Applied
Biology at the University of Parma, in the Department of Chemistry, Life Sciences, and
Environmental Sustainability. She is the President of the Degree in Biotechnology and member of the
PhD Course in Biotechnology and Life Sciences. Her teaching activities cover Cell and Animal
Biology, Environmental Biology, Applications of Transgenic Organisms.
The research activities are focused on two main topics:
- food integrity in the framework of several National research projects, with the application of
molecular biology and genomics to protection of quality and authenticity
- environmental biotechnologies, phytoremediation, effects of pollutants including nanoparticles as
emerging contaminants.
She authored over 250 publications. She is in the Editorial Board of the journals 'Environmental
Science and Pollution Research' and 'International Journal of Phytoremediation'.
Dr. Rosaria Rinaldi IMM-CNR, Lecce, IT
Rosaria Rinaldi graduated in Physics at the University of Bari (Italy) on July 1991. During her
Phd she worked on the optical and electronic properties of semiconductor quantum wires. She got
her PhD in 1994. From 1994 she has been working at the Material Science Department of
University of Lecce, where she builds up the facility for the fabrication of low dimensional
structures and photonic structures based on patterned materials. In January 2001, she was
appointed Professor in Condensed Matter Physics at the department of Innovative Engineering of
the University of Lecce. In 2001, she set up a Clean-Room for advanced nanoprocessing of
materials and realization of nanostructures based on Electron Beam Lithography, Galvanic
Electrodeposition of metals and Soft Lithographies. In the mean time, she opened a new research
line on nano- biotecnology and nano-bio-electronics. She is actually responsible of the “NEW
QUANTUM SYSTEMS” laboratory at DIE and "NANO - BIO-ELECTRONICS" division at the
National Nanotechnology Laboratory - INFM-CNR Lecce. She is author and co-author of more
than 200 articles published in peer reviewed international Journals.
3rd “PARMA” NANO-DAY, July 12-14, 2017
23 Chairmen
Prof. Antonio Mutti
Dept. of Medicine and Surgery, UniPR, Parma, IT
Graduated summa cum laude in Medicine and Surgery (1974), Specialist cum laude in Occupational
Medicine (1977), Resident (1974), then Research fellow (1976), and established university researcher
(1980), he is full Professor of Occupational Medicine at the University of Parma (2000). Responsible
of the Regional Reference Center of Industrial Toxicology (from 1997). Delegate for International
Relationships and Biomedical Research (from 1996 to 2000). From 2000, he is President of the
Committee for the assistance to the personnel of the University of Parma. Assistant director at the
Institute of Clinical Medicine and Nephrology (from 1991) then Department of Clinical Medicine,
Nephrology and Health Sciences (from 1996), of which he became Vice-director (2003-2010) and
then Director (1st November 2010). From 2006, he is member of the Board of the International
Commission on Occupational Health and member of Panel on Contaminants of the Food Chain at the
EFSA (European Food Safety Authority). Author of over 400 scientific publications, half of which in
peer-reviewed international journals he has been Project Leader or local Coordinator of the several
projects funded by the European Commission.
Sen. Prof. Giorgio Pagliari
Dept. of Law, Political and International Studies, UniPR, Parma, IT
He was elected Senator in 2013, he is professor of Administrative Law at the University of
Parma. He was involved in the important reform of the public administration to simplify
italian administration. According to Open Polis 2016 he is the most productive senator and
second within the Parliament. He is now a member of: Giunta delle elezioni e delle immunità
parlamentari, Commissione Affari Costituzionali, Commissione Giustizia, Comitato
parlamentare per i procedimenti di accusa, Commissione parlamentare per la
semplificazione, Commissione parlamentare di inchiesta sul rapimento e sulla morte di Aldo
Moro and President of Commissione contenziosa.
Dr. Andrea Zanlari President Camera di Commercio of Parma, Parma, IT
Dr. Zanlari is since 1999 president of the Camera di Commercio di Parma. In addition, he holds many
official offices, such as president of INDIS – Istituto Nazionale Distribuzione e Servizi, President of
PARMA ALIMENTARE.
He was nominated “Cavaliere dell’Ordine Costantiniano di San Giorgio, Commendatore della
Repubblica italiana and Grande Ufficiale dell’Ordine “Al Merito della Repubblica Italiana”.
3rd “PARMA” NANO-DAY, July 12-14, 2017
24 Invited Speakers
Dr. Ciro Chiappini Dept. of Materials, King's College, London, UK
Dr. Ciro Chiappini is Lecturer in Nanomaterials and Biointerfaces at King’s College London, United
Kingdom. He earned a PhD in Biomedical Engineering at the 3816, University of Texas at Austin
(USA) in 2011. He worked as Newton Fellow and Marie Curie Fellow at Imperial College London
until 2016. Dr. Chiappini develops functional interfaces for tissue engineeringand precision medicine.
Prof. Luca Vincetti Dept. of Engineering “Enzo Ferrari”, UniMORE, Modena-Reggio, IT
He received the Degree in Electronics Engineering (cum laude) from University of Parma, Italy. He
did his PhD in Information Technologies at the University of Parma on the development of numerical
methods for optical devices analysis and he got the PhD degree in 2000. He also received a Young
Research Project grant. The same year he became researcher at the National Inter-University
Consortium for Telecommunications. In 2001 he moved to the University of Modena and Reggio
Emilia with a grant funded by Cisco Research Center of Cisco Systems. The same year he became
Assistant Professor of Electromagnetic Fields at the Department of Information Engineering,
University of Modena and Reggio Emilia. He has made several key contributions in the development
and applications of analytical models of PMD in optical fiber and numerical models based on Finite
Element Method for the analysis and design of photonic components. He has also pioneered the use of
FEM modal solvers for the analysis of Photonic Crystal Fibers.
Prof. Daniele Ruggiu Dept. of Political Science, Law, and International Studies, UniPD, Padua, IT
Daniele Ruggiu is a legal philosopher at the Department of Political Science, Law, and International
Studies of the University of Padova where he teaches “Legal Theory”. He obtained his Ph.D degree at
the University of Palermo (2008). His main interests are: The European human rights law and the
work of the European Court of Human Rights; the impact of emerging technologies on human rights
(nanotechnology, synthetic biology, information technologies, human enhancement technologies); the
study of governance models, such as Responsible, Research and Innovation and the development of
rights-based models of governance; legal hermeneutics as the philosophical framework of human
rights. He worked on several EU funded projects (Synth-Ethics, EPOCH, ResAgora, NeroLaw
network etc.). He published articles in several Journals (Nanoethics, JLT, Ragion pratica, Rivista di
Filosofia del Diritto, Politeia) and took part to several conferences, national and international. He was
member of the “Center of research for legal, ethical and social decisions on emergent technologies” at
Padua University (2009-2015). He is member of the Group on “Ethics and Emerging technologies: a
Population-based Health Monitoring Project” of the Fondazione Lanza (Padova). He is member of the
editorial board of “Ars interpretandi. Journal of Legal Hermeneutics”.
3rd “PARMA” NANO-DAY, July 12-14, 2017
25 Invited Speakers
Dr. Dania Esposito Institute for Environmental Protection and Research, Rome, IT
Dr. Dania Esposito is a PhD, researcher of the Institute for Environmental Protection and Research-
ISPRA, in Rome. She is involved in the activities of national Competent Authority (CA) for the
implementation of Regulations concerning hazard and risk assessment of chemicals (i.e. “REACH”
and “CLP”), including environmental issues related to the Substance Evaluation process.
She is advisor of the Italian member of the Risk Assessment Commettee (RAC) at the European
Chemical Agency (ECHA). She takes part to national and European activities on challenges in
REACH implementation in relation on nanomaterials, focusing on the ecotoxicity and environmental
fate, with the aim to evaluate, define and propose advices to ensure the protection of the environment,
in relation to nanomaterials, supporting the national tasks at European level. Recently she was
nominated auxiliary expert for IT-CA in the advisory group ECHA-NanoMaterial Expert Group for
environmental aspects.
Prof. Cesare Galli Dept. of Law, Political and International Studies, UniPR, Parma, IT
Full Professor of IP Law, Law Department, University of Parma (1998-today), he is the author of
numerous publications, including the books: 3 D Printing. Legal aspects and business opportunities
(2014-2015, edited together with A Zama); The Commented Code of Industrial and Intellectual
Property (2011, edited together with A Gambino); The Italian IP Code: the 2010 Reform (2010); A
guide to the warranties on IP rights (2011); The New Frontiers of Patent Law (2003); The Future of
Trademarks in the Face of the Challenges of Globalization (2002); Functions of Trademarks and
Scope of Protection (1996). Since 2005 he has been a member of the Italian Government Boards of
Counsel in the IP field, including the board which drafted the 2010 reform of the Code of Industrial
Property. Since 2009 he has been a member of the sub-group on the legal framework of the EU
Counterfeiting and Piracy Observatory (now OHIM Observatory on the Infringement of IPRs). Head
and founder of IP-Law Galli (2004-today), a highly specialized IP boutique law firm, he handles
litigation involving all branches of IP law, often involving cross-border profiles, in particular
regarding the most cutting-edge sectors of technology.
Dr. Giovanna Zappa Research director at ENEA, Rome, IT
Giovanna Zappa, Research director at ENEA (Italian National Agency for New Technologies, Energy
and Environment) with more than thirty years of experience in Analytical Chemistry, Reference
Materials and Metrology applied to Chemical and Biological Measurements. She is Coordinator of the
European Research Infrastructure METROFOOD-RI and of the JRU METROFOOD-IT. Scientific
Responsible of National Research Project on food safety SAFE&SMART and principal Investigator of
research projects on food quality and safety. She is member of the IMEKO TC23 "Metrology in Food
and Nutrition", UNI and ISO Committees, Codex Alimentarius and academic teaching on "Risk
Analysis and Methodologies for Exposure Evaluation to Chemical Agents” graduation course in
“Techniques Environment and workplaces" – University of Rome “Tor Vergata”, tutor for graduation,
post-graduation, PhD and fellowships. She is author of more than 150 scientific publications.
3rd “PARMA” NANO-DAY, July 12-14, 2017
26 Invited Speakers
Prof. Luca Marchiol Dept. of Agricultural and Environmental Sciences, UniUD, Udine, IT
Luca Marchiol, PhD, is Associate Professor at the University of Udine (I). Director of 2nd degree in
"Land and Environment Science and Technology", joint Master between the University of Udine and
University of Trieste. Currently, LM teaches “Ecosystems Ecology” and “Restoration of Degraded
Sites”. As regards the research interests LM worked on plant ecophysiology and abiotic stress, then on
metal accumulators/hyperaccumulators vs phytoremediation. Since 2012 LM studies the relationships
between engineered nanomaterials and plants.
Dr. Isabella De Angelis Dept. of Environment and Health of the Italian National Institute of Health, Rome, IT
Isabella De Angelis, graduated in Biological Sciences, is researcher at the Department of Environment
and Health of the Italian National Institute of Health (ISS). Her research interests are focused on the
use and validation of in vitro models to investigate toxic action mechanisms. She is particularly
interested in chemicals and nanoparticles absorption processes using in vitro models of epithelial
barriers.
Since 2011, she is Head of Delegation at the OECD Working Party of Manufactured Nanomaterials
and member of the ISS working group “Nanomaterials and Health”. She actively participates to
National (Lazio Region, Ministry of Health) and European (NaNoREG, NanoReg2, EC4Safe Nano)
research projects addressed to nanomaterials safety.
Prof. Michele Miragoli Dept. of Medicine and Surgery, UniPR, Parma, IT
Tenure track assistant professor at the Department of Medicine and Surgery, University of Parma.
Research Associate at CNR-IRBG and group leader of the Cardiac Nanophysiology Laboratory at the
Humanitas Research Hospital, Milan. Currently, he holds the position of honorary research associate
at Imperial College London. 17 years of experience in cardiac physiology. Supervisor of different
M.Sc and PhD students. Dr. Miragoli is interested in cardiac nanomedicine and nanophysiology and
the group was among the first in demonstrating the interaction between excitable cardiac tissue and
charged nanoparticles. Principal Investigator and co-investigator of several national and international
project from Italian Ministry of Health, Italian Ministry of Education University and Research,
Wellcome Trust UK, British Heart Foundation UK, FP7 Capacities Program Qualityinano
Transnational Access, Horizon 2020. Awarded for the Italian Young Investigator Research Prize, from
the Italian Society of Cardiovascular Research and the National Institute of Cardiovascular Research
(2009) and from young investigation award led by the Swiss Society of Cardiology (2008). Co-author
of more than 40 papers published in international peer-reviewed journals and co-inventor of 1
international patent.
3rd “PARMA” NANO-DAY, July 12-14, 2017
28 Programme
Wednesday, 12 July
08:30 Registration (registration area)
09:00 Introduction of the organizers (congress hall)
Prof. Nelson Marmiroli (UniPR) and Dr. Salvatore Iannotta (IMEM-CNR)
Authorities welcome and remarks
09:30 Round Table “Technologies and Sustainable development”
Nanotechnologies are just one of the new technologies facing Industry and Universities
in their search on innovation. New applications create new perspectives in Italy and
abroad, opening up possibilities for researchers and operators in the field of science and
technologies, to be discussed for the safe and sustainable use of the knowledge.
Organizer
Prof. Nelson Marmiroli
Moderator
Vittoriano Zanolli - Direttore, “La Provincia” di Cremona e Crema
Speakers
Prof. Giovanni Franceschini - Rettore Vicario, UniPR
Dr. Simona Caselli - Assessore all'agricoltura, caccia e pesca, Regione E-R
Dr. Palma Costi - Assessore alle attività produttive, Regione E-R
Dr. Flavia Barone - Istituto Superiore di Sanità, Roma
Dr. Alberto Manzo - Ministero delle Politiche Agricole Alimentari e Forestali (MIPAAF)
Dr. Andrea Pontremoli - Dallara S.p.A., Parma
Prof. Paolo Cescon - IDPA-CNR, Venezia
Dr. Lanfranco Masotti - Presidente, Consorzio Italbiotec, Milano
Dr. Francesco Paolo Ausiello - Direttore Tecnico, ASTER
Prof. Antonio D’Aloia - Direttore Centro Bioetica, UniPR
13:00 Lunch break
14:00 Poster exhibition (poster area)
3rd “PARMA” NANO-DAY, July 12-14, 2017
29 Programme
14:30 Job Day (congress hall and Industry dedicated area, 1st floor)
“Industries meet students and young researchers”
Young participants will have the opportunity to meet Companies and Industries from the
sector of nanotechnologies, for discussion and interaction.
Oral communications (10 minutes) of selected companies that present themselves to the
audience by explaining the company’s Core Business, the Mission, the placement in the
national/international market, the main activities carried out, the possibility to offer
internships and apprenticeships for students and/or graduates and also provide contacts
and relevant business figures.
Moderator
Prof. Sara Rainieri (UniPR)
16:30 Project Session (congress hall)
Brief presentation of funded project in the area of nanotechnology.
Moderators
Prof. Enrico Dalcanale (INSTM) and Dr. Salvatore Iannotta (IMEM-CNR)
Projects
SUPRANANO – Prof. Enrico Dalcanale (INSTM)
INTENSE – Prof. Elena Maestri (UniPR)
TERMOREF – Prof. Francesco Basile (CIRI EA, UniPR)
Nano2Fun – Prof. Anna Painelli (UniPR)
SUPERSITO – Dr. Stefano Zauli Sajani (Center for Environment and Health, ARPAE E-R)
Smart-PV – Dr. Massimo Mazzer (IMEM-CNR)
Refrigerazione Magnetica – Dr. Simone Fabbrici (MIST E-R) and Dr. Franca Albertini
(IMEM-CNR)
3rd “PARMA” NANO-DAY, July 12-14, 2017
30 Programme
Thursday, 13 July
08:30 Registration (registration area)
Session I. Technologies and Applications (congress hall)
Chairmen: Prof. Roberto De Renzi (UniPR) and Prof. Rinaldo Garziera (UniPR)
09:00 Plenary lecture - Dr. Ciro Chiappini (King’s College London, UK).
Engineering high aspect ratio nanostructures to direct cell fate
09:25 Oral Communication - Prof. Daniele Pontiroli (UniPR).
Carbon Nanostructures for Energy Applications
09:40 Oral Communication - Dr. Simone Fortunati (UniPR).
Amperometric genosensor based on PNA probes implemented on carbon nanotubes-
modified screen printed electrodes
09:55 Oral Communication - Dr. Valeria Caponetti (UniBO).
Fluorescent nanoaggregates by self-assembly of amphiphilic perylene bisimides for
sensing pH and anions in water
10:10 Oral Communication - Prof. Rosaria Rinaldi (IMM-CNR).
Multi-shape hydrogel-nanostructures: microfluidic-assisted approach
10:25 Oral Communication - Dr. Carlo Molardi (UniPR).
Design of micro-structured fiber for improved high power laser operation
10:40 Oral Communication - Dr. Carlotta Peruzzi (IMEM-CNR).
Full plastic organic electrochemical transistors with graphene-metal nanoparticle
composites as gate electrodes: a benchmark towards the intrinsic selectivity
11:00 Coffee break
11:20 Invited speaker - Prof. Luca Vincetti (UniMORE).
Microstructured Fibers for label-free DNA detection
11:40 Poster Presentation Session A. Technologies, Chemistry and Physics (congress hall)
Chairmen: Prof. Maria Careri (UniPR) and Dr. Andrea Zappettini (IMEM-CNR)
13:00 Lunch break
13:45 Poster vision and evaluation (poster area)
3rd “PARMA” NANO-DAY, July 12-14, 2017
31 Programme
Session II. Regulation and Economics (congress hall)
Chairmen: Prof. Luca Di Nella (UniPR) and Dr. Soren Bowadt (European Commision, DG Research
& Innovation)
14:30 Plenary lecture - Dr. Soren Bowadt (European Commision)
15:00 Miniplenary - Prof. Daniele Ruggiu (UniPD).
Towards a overall regulation of nanotechnologies: is the devil in the detail?
15:20 Invited speaker - Dr. Dania Esposito (ISPRA, Roma).
Nanomaterials and EU Regulation on Chemicals. Approach to the environmental risk
assessment
15:35 Invited speaker - Prof. Cesare Galli (UniPR).
IP Protection of Nanotechnology: Patents Vs Open Source - Pros and Cons
15:50 Oral Communication - Dr. Maria Chiara Errigo (UniPR).
The Nanotechnologies in the food sector: the European regulation
16:05 Short Communication - Prof. Elena Maestri (UniPR).
Nanotechnologies in agri-food: lessons learned from transgenic organisms
16:15 Coffee break
16:40
Poster Presentation Session B. Medicine, Health, Drugs and Veterinary Medicine
(congress hall)
Chairmen: Prof. Ruggero Bettini (UniPR) and Prof. Giorgio Dieci (UniPR)
3rd “PARMA” NANO-DAY, July 12-14, 2017
32 Programme
Friday, 14 July
08:30 Registration (registration area)
Session III. Agri-food, Environment and Biotechnologies (congress hall)
Chairmen: Prof. Marta Marmiroli (UniPR) and Dr. Jason C. White (The Connecticut Agricultural
Experiment Station, CAES; New Haven, CT, USA)
09:00 Plenary lecture - Dr. Jason C. White (CAES, USA).
Nanomaterials and the Food Supply: Assessing the Balance Between Applications and
Implications
09:30 Miniplenary - Dr. Giovanna Zappa (ENEA)
Application of nanotechology in agri-food: opportunities and challenges
09:50 Oral Communication - Dr. Michela Janni (IMEM-CNR).
Bioristor: an in vivo biosensing, biomimetic electrochemical transistor with applications
in plant science and precision farming
10:05 Oral Communication - Dr. Alessia Favero (UniPR).
Tetraphosphonate Cavitands recognition in water: from amino acids to histone
camouflage
10:20 Oral Communication - Dr. Manon Auguste (UniGE).
PANDORA: probing safety of nano-objects by defining immune responses of
environmental organisms
10:35 Oral Communication - Dr. Chiara Lico (ENEA).
A biodistribution study of two differently shaped plant virus nanoparticles reveals new
peculiar traits
10:50 Short communication - Dr. Luca Pagano (UniPR).
Physiological and molecular response Cucurbita pepo exposed to ENM binary
combinations
11:00 Coffee break
11:20 Invited speaker - Prof. Luca Marchiol (UniUD).
Phytonanotechnology: a new horizon to food security
11:40 Poster Presentation Session C. Agri-food, Environment and Biotechnologies
(congress hall)
Chairmen: Dr. Rita Baraldi (IBIMET-CNR) and Prof. Elena Maestri (UniPR)
13:00 Lunch break
13:45 Poster vision and evaluation (poster area)
3rd “PARMA” NANO-DAY, July 12-14, 2017
33 Programme
Session IV. Medicine and Health (congress hall)
Chairmen: Prof. Rosaria Rinaldi (IMM-CNR) and Prof. Antonio Mutti (UniPR)
14:30 Plenary lecture - Dr. Isabella De Angelis (Istituto Superiore di Sanità)
Approaches for nanomaterial safety
15:00 Miniplenary - Prof. Michele Miragoli (UniPR).
Recovery of cardiac impulse propagation in ischemic rat heart via silicon carbide
conductive nanowires (SiC-NW)
15:20 Oral Communication - Dr. Gregorio Marchiori (Rizzoli Orthopedic Institute).
3D printing of novel PCL/bioactive glass scaffolds for bone tissue regeneration:
nanoindentation and finite element analyses
15:35 Oral Communication - Dr. Alessandra Procopio (UniBO).
Quantitative determination of dye doped silica nanoparticles in cancer cells
15:50 Oral Communication - Dr. Nicolò Riboni (UniPR).
Odorant binding protein-functionalized superparamagnetic nanoparticles for biomedical
applications
16:05 Oral Communication - Dr. Gloria Zaccariello (UniVE).
Growth of Bismuth Titanate into mesoporous silica nanoparticles: a new safe self-sealing
UV filter for cosmetic formulations
16:20 Short Communication - Dr. Laura Paesano (UniPR).
MicroRNAs transcriptional regulation in human cells exposed to CdS QDs
16:30 Poster awards (congress hall)
Chairmen: Prof. Giorgio Pagliari (Senatore) and Dr. Andrea Zanlari (Presidente, Camera
di Commercio Parma)
17:00 Conference closure by organizers
18:00 Scientific Happy Hour
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 36 Oral Communication - Session I
Engineering vertical nanowire arrays for cell manipulation
C. Chiappini1
1Department of Craniofacial Development and Stem Cell Biology, Dental Institute
King's College London, United Kingdom.
Engineering the cell-material interface to direct cell fate carries transformative potential for
regenerative medicine, precision medicine and fundamental cell and molecular biology.
Vertical arrays of nanowires and nanoneedles are rapidly emerging as promising biointerfaces
capable of manipulating cells. Thanks to their high aspect ratio, nanoneedles interact
simultaneously with multiple organelles within the cell, enabling gene delivery, intracellular
sensing, and direct stimulation of signalling pathways at multiple levels of the
mechanosensory machinery. Leveraging these interactions enables gene therapy, epigenetic
remodelling and intracellular sensing, yielding dynamic manipulation of cells and their
environment with broad impact both in vitro and in vivo.
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 37 Oral Communication - Session I
Carbon Nanostructures for Energy Applications
D. Pontiroli1*
, G. Magnani1, S. Scaravonati
1, D. D’Alessio
1, M. Gaboardi
1,2,
C. Milanese3, G. Bertoni
4, A. Malcevschi
5, N. Sharma
6, M. Riccò
1
1 CNL, DSMFI, University of Parma, Parco Area delle Scienze 7/A, 43124 Parma Italy.
2 ISIS Facility, Rutherford Appleton Laboratory, Chilton, Didcot, Oxfordshire OX11 0QX, United Kingdom.
3 Pavia Hydrogen Lab, University of Pavia, Viale Taramelli 16, 27100 Pavia (Italy).
4 IMEM-CNR, Parco Area delle Scienze 37/A, 43124 Parma, Italy.
5 DSCVSA, University of Parma, Parco Area delle Scienze 11/A, 43124 Parma Italy.
6 School of Chemistry, UNSW Australia, Sydney NSW 2052, Australia.
The progressive depletion of fossil fuels and the subsequent need to quickly switch to
renewable sources strongly require to boost the performances of the current energy-storage
systems. In fact, on the one hand, efficient large-scale electric energy accumulators will be
implemented in future smart-grids to buffer the intermittent production of energy by sun and
wind power. On the other hand, the production of electric vehicles will need even more
performing devices for both energy harvesting and storage, in order to become really
competitive with existing petrol cars.
In this scenario, the development of innovative technologies exploiting novel carbon
nanostructures could be the winning strategy, thanks to their unique mechanical, electronic
and structural properties and to the intrinsic environment-friendly character of carbon.
In this talk, a survey of the recent advances in the field of the energy transport, conversion
and storage, obtained at the Carbon Nanostructures Laboratory (CNL) at the Department of
Mathematical, Physical and Computer Sciences at the University of Parma, is given. Carbon
nanomaterials, based on Buckminster-fullerene, graphene and activated bio-chars,
demonstrated to be promising for the development of novel Li-/Na-/Mg-ion batteries,
supercapacitors and direct/indirect solid-state hydrogen-storage systems.
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 38 Oral Communication - Session I
Amperometric genosensor based on PNA probes implemented on carbon
nanotubes-modified screen printed electrodes
S. Fortunati, M. Giannetto, A. Rozzi, M. Mattarozzi, A. Manicardi, R. Corradini, M. Careri
Dipartimento Scienze Chimiche, della Vita e della Sostenibilità Ambientale, Università di Parma, Parco Area
delle Scienze 17/A 43124 Parma.
Genosensors have received great attention in the past decade, being nucleic acids promising
molecular probes due to the ease of functional modification and the specificity for base
pairing. In a program dealing with the development of innovative sensors as analytical tools
for assessing food safety1,2
we combined DNA-mimic probes based on Peptide Nucleic Acids
(PNAs) with the enhancing properties of Carbon NanoTubes as binding substrates on Screen
Printed Electrodes (CNT-SPEs). Our previous studies3, focused on the use of the same PNA
probes on all-gold SPEs, evidenced that best results have been obtained suiting a non-
competitive approach, based on the binding of target “Roundup-Ready (RR)” transgenic soy
DNA by a PNA-Capture Probe (CP)-functionalized sensor, followed by the hybridization
with a PNA-Reporter Probe (RP). The latter bears a biotin tag, capable of strong interactions
with a streptavidin-Alkaline Phosphatase conjugate, which converts a substrate into an
electroactive species. We experimented CNT-SPEs obtaining higher loading capability of CP,
if compared to gold substrates. Another crucial aspect deals with the nature of the RP, since
we noticed the formation of CP/Target/RP adduct using PNAs both for capture and reporter
probes. To overcome this limit, we moved to DNA-based RPs, obtaining encouraging results
in terms of signal inhibition associated to hybridization of the target DNA up to nanomolar
scale. Further studies, currently ongoing, are focused on the comparison of such inhibitive
approach with the use of a sandwich-type assay based on the use of a longer DNA target,
hetero-complementary to CP and RP.
[1] A. Manfredi, M. Giannetto, M. Mattarozzi, M. Costantini, C. Mucchino, M. Careri, Anal Bioanal Chem
(2016) 7289–7298
[2] M. Giannetto, E. Umiltà, M. Careri, Anal. Chim. Acta 806 (2014) 197-203.
[3] S. Fortunati, MsSCI thesis, 2016.
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 39 Oral Communication - Session I
Fluorescent nanoaggregates by self-assembly of amphiphilic perylene
bisimides for sensing pH and anions in water
V. Caponetti1*
, M. Montalti2
1University of Bologna, via Selmi 42, Bologna, Italy.
2University of Bologna, via Selmi 42, Bologna, Italy.
Multi-stimuli responsive materials are finding increasing importance in fields of high social
and economic impact that include drug delivery, diagnostics, tissue engineering and ‘smart’
optical systems, as well as microelectronics, biosensors, microelectromechanical systems,
coatings and textiles. Although different design approaches have been proposed, the self-
assembly of molecular or nanostructured building blocks is, without any doubt, one of the
most versatile, straightforward and powerful strategy to achieve stimuli-responsive materials.
The response of these materials, either to environmental or external solicitation, can be, in
fact, achieved by exploiting the same inter-components interactions that brings to their
assembly.
Here we demonstrate that aggregation of properly designed perylene bisimide amino derivate
can be controlled by pH in
water solution. As shown in
the figure the dye molecules
(a) aggregate in water
because of p-p stacking
interaction (b), causing
fluorescence quenching.
The system can be
disaggregated and the
fluorescence switched on
by protonation exploiting
the electrostatic repulsion
between the positive cations
(c).
Even more interestingly we
observed that the
protonated system can be re-assembled by increasing the concentration of anion that partially
shield the positive charge of the ammonium substituents.
Finally, our molecules show a unique response to protonation and anion concentration in
water. These features make them very promising tools for ion sensing in view of biological
and environmental applications.
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 40 Oral Communication - Session I
Multi-shape hydrogel-nanostructures: microfluidic-assisted approach
A. Aloisi1, C.C. Toma
2, G. Mandriota
2, R. Di Corato
2, R. Rinaldi
1,2,*
1CNR Institute for Microelectronics and Microsystems, SP Lecce-Monteroni, Lecce, Italy.
2Dipartimento di Matematica e Fisica "Ennio De Giorgi", Università del Salento, Via Arnesano, Lecce, Italy.
Here we present a microfluidic-assisted method of nano-vesicles assembly under sterile,
closed environment and gas exchange adjustable conditions, a critical issue, when the cargo to
be upload is very sensitive. Conventional approaches to synthesize sodium alginate-based
vesicles in aqueous phase relies on bulk mixing of polymer and divalent cations. Here we
propose the option to simply tuning diverse shapes of organic/organic-inorganic bio-hybrid
systems for pharmaceutical formulations/biomedical applications, adjusting chemical and
physical forces. Fine-tuning stream flow rate, microchannel surface chemistry and salts
concentration, we succeed in optimizing shape-controlled calcium alginate nanogels (NGs)
polymerization process, at interface of the glass/PDMS cross-shaped microfluidic device.
Furthermore, as, active molecules cargo amount mainly influences the final -drug delivery
device- product costs, one of the purposes of the novel method is to obtain a very efficient
drug encapsulation process, in order to improve the efficiency of encapsulated active
drug/vesicle volume ratio, even starting from very low cargo concentrations [1].
Given the undoubtedly favorable properties of hydrogels, different regulatory issues should
turn into a relevant starting point for discussion among worldwide regulatory bodies, drug
policymarkers, and biopharmaceutical companies in pursuing suitable pharmaceutical product
formulation based on biopolymer like alginate, chitosan and their derivatives.
[1] F. Sallustio, C. Curci, A. Aloisi, C.C. Toma, E. Marulli, G. Serino, S. Cox, G. De Palma, A. Stasi, C. Divella,
R. Rinaldi, F.P. Schena. Inhibin-A and Decorin secreted by humanadult renal stern/progenitor cells through the
TLR2 engagement induce renal tubular cell regeneration. Accepted for publication in Scientific Reports.
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 41 Oral Communication - Session I
Design of micro-structured fiber for improved high power laser operation
C. Molardi1, F. Poli
1, A. Cucinotta
1, S. Selleri
1*
1Department of Engineering and Architecture, University of Parma, Parco Area delle Scienze 181/A I-43124,
Parma, Italy.
In recent years fiber laser systems have shown a rapid evolution in terms of beam quality and
power. To achieve high pulse energy and peak power, a large effective area is required, so
new photonic crystal fiber designs have been proposed. Thermo-optical effects can lead to
transverse mode instability, a nonlinear effect which suddenly appears at certain threshold of
power. In this contribution, improved fiber designs for Yb-doped power amplifiers are
proposed. The fibers are numerically investigated focusing on the influence of thermal effects
on guidance properties. The analysis has been obtained with a custom software based on finite
element method, provided with a spatial amplifier combined with an efficient thermal model.
Two kind of fibers with micro-structured cladding have been investigated. The first, a large
pitch fiber, is based on a periodic pattern of hole inside the cladding. The second, a symmetry
free photonic crystal fiber, is based on aperiodic cladding structure. These fibers present a 19-
cell doped core with an area of 1178.47 μm2, a double cladding structure obtained with an air-
cladding characterized by a diameter of 270 μm, and a length of 0.9 m. The fibers have been
pumped with a 400 W pump at 976 nm. The input signals, composed by the fundamental
mode of 5 W and the first higher order mode of 50 mW, have been considered at 1032 nm.
Results show the possibility of achieving an effective amplification, maintaining an output
mode discrimination larger than 0.56.
Fig.1. (a) SEM image of a micro-structured all-solid photonic crystal fiber; (b) SEM image of the large pitch
fiber; (c) Schematic of the cross-section of the symmetry free photonic crystal fiber, with a detail of the mesh
used for numerical simulations.
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 42 Oral Communication - Session I
Full plastic organic elecrochemical transistors with graphene-metal
nanoparticle composites as gate electrodes: a benchmark towards the
intrinsic selectivity
C. Peruzzi1,2
, S. Pascale3,4
, P. D’Angelo1, G. Tarabella
1, S. Marasso
1,5, M. Cocuzza
1,5, A. Secchi
2, F.
Fabbri1,3
, S. Iannotta1
1IMEM-CNR Institute, Parco Area delleScienze 37/A, 43124, Parma, Italy.
2 Department of Chemistry, University of Parma, Parco Area delleScienze, 43124, Parma, Italy.
3 KET – Lab, c/c Italian Space Agency, Via del Politecnico, 00133, Roma, Italy.
4Hypatia Consortium, c/c Italian Space Agency, Via del Politecnico, 00133 Roma, Italy.
5Chilab, Materials and Microsystems Laboratory, Department of Applied Science and Technology (DISAT),
Politecnico di Torino, Via Lungo Piazza d'Armi 6 10034 Chivasso, Torino, Italy.
Organic Electrochemical Transistors (OECTs) are a special class of bio-electronic devices.
The peculiar features of the organic conductor constituting the OECT active channel allow
developing versatile devices with multifunctional operation, even if the OECT primary field
of action is Biosensing. OECTs are capable of detecting a large variety of bioanalytes via
modulation of the active channel current, also in real-time and with a sensitivity often
exceeding that of everyday biosensors. However, even if the demonstrated high
transconductance of OECTs allows amplifying small variations of ionic signals, the concrete
detection of bioanalytes dissolved in a physiological ambient is not an easy task, due to the
lack of intrinsic specificity.
We show the fabrication and characterization of a Graphene-metal nanoparticles composite
and its implementation as a flexible gate electrode in OECTs. Prism-shaped Ag nanoparticles
are physisorbed on Graphene/Low Density Polyethylene substrates at different concentrations
and the deposition process has been monitored by different experimental techniques (Uv-Vis
spectroscopy, TEM, SEM, DLS and Zeta-Potential). The effect of the as-prepared gate
electrodes on the OECT response in presence of a physiological ambient has been studied by
collecting transconductance curves as a function of the gate voltage. We show that our gates
furnish a tool for resolving the contribution of a specific species present in the saline ambient
to the overall signal. Our gates promote the decoupling of the faradaic signal of salts in
solution, activated by the metal counterpart of the nanocomposite and amplified by the
transducing element, and the contribution of the graphene support.
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 43 Oral Communication - Session I
Microstructured Fibers for label-free DNA detection
L. Vincetti1,*
, F. Giovanardi1, A. Cucinotta
2
1Department of Engineering “Enzo Ferrari”, University of Modena and Reggio Emilia, via Vivarelli 10, 41124
Modena, Italy. 2Department of Engineering and Architecture, University of Parma, Parco Area delle Scienze, 181/A, 43124
Parma, Italy
Photonic Crystal Fibres (PCFs) represent a promising platform for the development of cost-
effective and sensitive sensors for the detection of specific DNA sequence. Solid Core PCFs
and Hollow Core Photonic Band Gap PCFs have been numerically and experimentally
investigated for this purpose. The air holes running along the fibre length allow the
infiltration of biologically active substances into the fibre. Biological layers are so attached
at the air-dielectric interfaces. Inhibited Coupling (IC) fibres are a new kind of Hollow Core-
PCFs where the particular microstuctured cladding guarantees the inhibition coupling
between core modes and cladding modes at particular wavelengths. This results in a
transmission spectrum composed by an alternation of high and low loss regions. The
nanometric thickness of the biological layer results in a shift of the transmission spectrum.
This shift can be exploited in order to obtain a sensor without the need of any grating.
Fig. Top: Fibre cross section and detail of a single tube composing the microstructured cladding. Bottom: loss
of a 20cm long fibre without bio-layer, and with bio-layer 10nm, 20nm, and 30 nm thick
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 44 Oral Communication - Session II
Towards a overall regulation of nanotechnologies: is the devil in the detail?
D. Ruggiu1*
1Department of Political Science, Law, and International Studies, University of Padova, Italy.
Nanotechnology is at its mature stage. Products based on nanotechnologies are increasingly
marketed. Science has reduced the level of uncertainty and gained new knowledge in this
sector. In the meantime, a law-making process occurred. The EU started to regulate
nanotechnologies since 2000s. Cosmetics, electronics, food labelling, biocides, waste
electrical and electronic equipment, medical devices have been progressively regulated at EU
level. We are in the middle of a process of consolidating the European regulatory landscape in
this sector which paradoxically resulted from an initial approach inspired by the new
governance paradigm. However, this detailed regulatory movement caused lacunae,
inconsistencies, incoherencies which compromises EU efforts in this field. There is the need
for uniform criteria which are able to shape a multitude of regulatory tools as part of a smart
regulation. These criteria are EU fundamental rights, and they can be the starting point for a
novel type of business, which is competitive and ethically sound.
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 45 Oral Communication - Session II
Nanomaterials and EU Regulation on Chemicals. Approach to the
environmental risk assessment
D. Esposito1,*
1 National Institute for Environmental Protection and Research (ISPRA), Via V. Brancati 48 - 0144, Rome, Italy.
The rapidly expanding Nanotechnology provides technical and commercial opportunities,
however nanomaterials may pose risks to the environment and raise health and safety
concerns for humans and environment connected to their innovative properties.
According to the main EU regulation on chemicals, the REACH Regulation (EC) No
1907/2006, manufacturers, importers and downstream users must ensure the safe use of each
substance, whatever its form. Therefore, the processes foreseen by REACH (e.g. registration,
evaluation, authorisation and restrictions), together with classification and labelling processes,
(according CLP Regulation (EC) No 1272/2008), involve nanoforms as any other form of a
substance although there are no explicit requirements for nanomaterials. This imply to
adequately asses and manage the potential risks still not well defined of these forms of
materials, introducing new challenges for regulators, such as the Commission and European
Chemical Agency (ECHA), Member State Competent Authorities, as well as all other
stakeholders.
In particular, because one of the aims of the REACH Regulation is to fill the knowledge gap
on chemicals, it is important to provide advice on any scientific and technical issues regarding
the implementation of REACH and CLP legislation in relation to nanomaterials.
It is presented a focus on the main environmental issues, in term of hazard and exposure
information requirements, related to specificity of nanomaterial properties, as it is addressed
at European level.
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 46 Oral Communication - Session II
The nanotechnologies in the food sector: the European regulation
M.C. Errigo, M. Tempesta
1University of Parma, Parma, Italy.
Nanotechnologies industrial applications are diversified and concern the most important
business sectors with inevitable and significant economic impacts; first, in the field of food
production and storage. Producing feeds that have particular qualities, giving particular colour
to foods, enriching them with special additives, ensuring their wholesomeness by proper
packing, are some of the activities in which nanotechnologies can have a significant and
positive incidence. At the same time, because of their complexity, nanomaterials need to be
subjected to scrupulous control to reduce the risk of human health. Therefore, the law have to
mediate, even through the use of the precautionary principle, between the requirement of
technological innovation and the protection of human health, and finds a very interesting test
ground in the use of nanotechnologies in the food sector.
Consequently, the purpose of the proposed intervention is to study EU legislation on
the subject in question, trying to verify how this intervention has been carried out at the
institutional level (also in terms of its democratic legitimacy). So, the concepts of "soft law",
"new governance" and "accountability", which are crucial to understanding how the law - in
particular, the "European law" - "reacts" to the rapid evolution of technological development,
will be studied with special attention. More specifically, we will try to describe the role
played by European institutions - also by looking at their mutual relationships - without
forgetting, of course, the influence exercised by EFSA.
Following the definition of the general framework, the proposed speech will focus on
details of the regulation at European level.
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 47 Oral Communication - Session II
Nanotechnologies in agri-food: lessons learned from transgenic organisms
E. Maestri1,2
, R. Ruotolo1*
, M. Marmiroli1, N. Marmiroli
1,2
1University of Parma, Department Chemistry, Life Sciences, Environmental Sustainability, Parco Area delle
Scienze 11/A, Parma, Italy. 2CINSA, Parco Area delle Scienze, Parma, Italy.
Nanotechnology is a rapidly evolving field with the potential to forward agriculture and food
industry with new tools to improve food production and processing along the entire supply
chain, improving agricultural practices, industrial processes and analytical techniques but also
the shelf-life of fresh and processed products, plant performance, nutrient absorption and pest
resistance. The applicability of a nanotechnology-based farming, built upon the experience
and practices of different countries, must be pursued in order to increase sustainability,
without damaging soil and water resources, and without causing carbon/nitrogen loss due to
leaching and emissions. Similar exciting perspectives have often been prospected for
transgenic plants in agriculture, and have been subjected to wide debate.
Nanotechnology-based food products and food packaging materials are already available to
consumers in some countries, like titanium dioxide in sweets or metals in supplements, but
additional products and applications are currently at the research and development stage.
Some have reached a high level of technology readiness. A broad range of nanotechnology-
derived products will be increasingly available to stakeholders, agri-food business operators,
food industry and consumers in the near future. In this situation, existing data and knowledge
available on the effects of nanomaterials in humans, crop plants and livestock are not yet
sufficient to allow for a thorough evaluation of their potential and of their safety. Evidence
required for effective regulation and governance must be delivered, and full knowledge
exchange and dissemination programme with key stakeholders and end-users, including the
general public, must be implemented, to avoid some of the mistakes which characterised the
early introduction of transgenic crops.
A consideration about the history of introduction of transgenic organisms in agriculture from
the 1990s can provide useful information and lessons on mistakes to be avoided during the
introduction of nanotechnology in agriculture and food. The attitude of EU institutions and
bodies towards nanotechnologies will be described, in view of application of the
precautionary principle and of implementation of risk assessment procedures. Promises and
pitfalls of nano-agri-food will be examined.
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 48 Oral Communication - Session III
Nanomaterials and the food supply: assessing the balance between
applications and implications
J.C. White
2Connecticut Agricultural Experiment Station, 123 Huntington Street, New Haven CT 06511, USA.
Increasing population growth and a changing climate will exert significant pressure on
already stressed agricultural production systems. Nanotechnology has the potential to
dramatically increase global food production and food security. However, any widespread use
of nanomaterials in food production must occur with a thorough understanding of both the
risks and benefits, including an assessment of the fate and effects of these materials in the
environment. Work at the Connecticut Agricultural Experiment Station is focused both on
novel applications of nanomaterials (NM) to suppress crop disease, as well as on efforts to
understand the mechanisms of NM toxicity on biota. A synopsis of these two complementary
programs will be presented.
Plant pathogens reduce agricultural productivity by 20%, resulting in billions of dollars of
losses from reduced yield, wasted resources, and compromised food quality; novel
approaches to suppress crop disease are clearly needed. Plant micronutrients are critical to
disease resistance but availability in soil is low and foliarly applications are often poorly
absorbed/translocated. However, nanoparticle (NP) forms of nutrients may be absorbed and
translocated more effectively. Greenhouse and field investigations have shown that nanoscale
micronutrients can increase the growth and yield of tomato, eggplant and watermelon in soil
infested with the pathogens Verticillium or Fusarium. Current efforts are focused on
understanding the mechanisms of disease suppression, as well as on optimizing amendment
strategies to maximize food production.
Significant effort is being made to understand the fate and effects of NM in agricultural
systems so as to ensure the sustainable application of nanotechnology in agriculture.
Experiments are focused on three areas: assessing the molecular mechanisms of NP
phytotoxicity, evaluating the trophic transfer of NP within the food chain, and characterizing
the effect of NP exposure on the fate co-contaminants. Proteomic and transcriptomic data
indicate that plant response to NP exposure and subsequent trophic transfer is often
significantly different from corresponding bulk/ionic materials. NP co-exposure can also
significantly alter co-contaminant accumulation and toxicity, but these effects again vary
significantly with the conditions of exposure. Collectively, this work shows that although
NP/NM use can significantly increase food production, important questions remain
unanswered and any application of nanotechnology in agriculture should only be pursued with
a sufficient understanding of mechanisms of action.
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 49 Oral Communication - Session III
Application of nanotechnology in agrifood: opportunities and challenges
G. Zappa
Italian National Agency for New technology, Energy and Sustainable Economic Development
Department for Sustainability – Biotechnology and Agroindustry Division
C. R. Casaccia Via Anguillarese 301, Rome ITALY, City, Country.
The introduction of nanotechnologies in food industry and agriculture paved the way for a lot
of promising applications, at all the stages of the supply chain. Nanotechnologies could play
an important role in establishing a more sustainable supply of high-quality food products for
the global population. In primary production nanotechnologies allow to realize nanoformulate
pesticides, herbicides, or veterinary drugs with slow release characteristics or triggered release
in response to a specific hazard. At the stage of food processing, nanotechnologies permit a
more efficient fractionation of crops, and - by using nano-encapsulation - to deliver
ingredients to specific locations in the gastrointestinal walls or to improve bioavailability of
nutriceuticals. At now, the most advanced applications of nanotechnologies concern
packaging. Thanks to nanotechnologies it is possible to develop advanced food contact
materials and active, smart and biodegradable packaging. Other very important applications
are related to sensor development.
The application of nanotechnology offers the possibility to improve both the safety (in terms
of increased shelf life and better monitoring) and the quality (in terms of taste, texture,
firmness and nutritional value) of products, as well as to apply Controlled Environment
Agriculture and Precision Farming techniques.
In parallel, the rapid proliferation and spread of nanotechnologies in a wide range of
consumer products, raised a number of concerns related to the protection of public health,
food safety, environmental protection and ethical, political and regulatory issues.
Metrology has a key role in the development and introduction of new technologies, both to
facilitate their application, to assess the risks and to measure the benefits introduced.
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 50 Oral Communication - Session III
Bioristor: an in vivo biosensing, biomimetic electrochemical transistor with
applications in plant science and precision farming
M. Janni1,2
, N. Coppedè1, M. Bettelli
1, F. Gentile
3, A. Petrozza
4, F. Cellini
4, N. Marmiroli
5, A.
Zappettini1,*
11Institute of Materials for Electronics and Magnetism (IMEM), National Research Council (CNR), Parco Area
delle Scienze 37/A, 43124 Parma, Italy. 2Institute of Bioscience and Bioresources (IBBR), National Research Council (CNR), Via Amendola 165/A,
70126 Bari, Italy. 3Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parco Area
delle Scienze, 11/A, 43100 Parma, Italy. 4 Centro Ricerche Metapontum Agrobios. S.S. Jonica 106 Km. 448.2, Metaponto, Italy.
5Department of Electrical Engineering and Information Technology, University Federico II, Naples, Italy.
The ability to monitor the plant physiological activity using in vivo and integrated biosensors
represents a key point for SMART farming.
Different approaches have been proposed to analyse the plant physiological activity on a
qualitative basis but few data are available in terms of quantitative data. Biosensors are tools
that transform a recognition event such as the perception of a small molecule by a receptor
into a signal that can be easily detected and quantified.
Here we present a biomimetic in-vivo biosensor named “Bioristor” that monitors qualitative
and quantitative changes in tomato sap during growth and development directly integrated
within the plant tissues. It is an active part of the device and resulted perfectly integrated
within the plant tissues without altering tomato morphology. The data analyses showed that
the device detects the changes that occur in the sap composition and concentration and
allowed to do a perfect match with the photoperiodic cycle. Moreover, the application of
bioristor in tomato plants in low water conditions allow an early detection of the plant stress.
The sensor has been also used in an integrated experiment in the automated phenotyping
platform held in ALSIA on tomato plants subjected to drought and salt stress allowing to
couple the imaging data obtained in the platform with the bioristor data giving a 360-degree
view on tomato development and physiology under stress.
The developed user-friendly, low cost biosensor able to detect stresses in early stages, open
new perspectives for precision farming and plant science studies.
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 51 Oral Communication - Session III
Tetraphosphonate cavitands recognition in water: from amino acids to
histone camouflage
A. Favero1, R. Pinalli
1, G. Brancatelli
2, D. Menozzi
1, D. Hernández
3, P. Ballester
3, S. Geremia
2, E.
Dalcanale1,*
1 Department of Chemistry, University of Parma, Parco Area delle Scienze 17/A, 43124 Parma, Italy.
2 Department of Chemical and Pharmaceutical Sciences, University of Trieste, via Giorgeri 1, 34127 Trieste,
Italy. 3 Institute of Chemical Research of Catalonia (ICIQ), Avgda. Països Catalans 16, 43007 Tarragona, Spain.
Amino acids (AA) represent an ideal playground for testing complexation ability and
selectivity of synthetic receptors, due to their biological relevance and chemical diversity.1 In
particular, tetraphosphonate cavitands (Tiiii) present remarkable molecular recognition
properties towards N-methylammonium salts.2 The origin of Tiiii selectivity towards these
species can be attributed to the presence of three synergistic interaction modes: (i) N+•••O=P
cation–dipole interactions; (ii) cation-π interactions of the acidic +N–CH3 group with the π
basic cavity; (iii) two simultaneous hydrogen bonds between adjacent P=O bridges and the
nitrogen protons. The ability of Tiiii in complexing monomethylated AA was studied both in
solid state, through single crystal X-ray diffraction, and in solution, via NMR and ITC
experiments. The aims of the reported study are: (i) to highlight the non-covalent interactions
involved in the binding event in each case; (ii) to investigate Tiiii ability to discriminate
between the different AA; (iii) to calculate the Ka values of the different complexes,
dissecting the entropic and enthalpic contributions; (iv) to determine the solvent influence on
the complexation selectivity. The solution experiments show that, moving from methanol to
water, the complexation event changes from entropy driven to entropy opposed, leading to a
drop of the Ka. However, this reduction in binding affinity is associated with an increase in
selectivity, since in aqueous solutions only N-methylated amino acids are effectively
recognized. These results open the way for the use of tetraphosphonate cavitands in the
detection of post-translational monomethylation of lysine residues in histones.4
References 1 Späth, A.; König, B. Beilstein J. Org. Chem. 2010, 6, No. 32.
2 Dionisio, M. et al. J. Am. Chem. Soc. 2012, 134, 2392-2398.
3 Pinalli, R. et al. J. Am. Chem. Soc. 2016, 138, 8569.
4 Bontempi, N. et al. NanoScale. 2017, DOI: 10.1039/C7NR02491F.
Figure 1. Molecular structures and ITC data for Nε-Me-Lys complexation with Tiiii in MeOH vs water 3
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 52 Oral Communication - Session III
PANDORA: probing safety of nano-objects by defining immune responses
of environmental organisms
M. Auguste1, L. Canesi
1,*
1 Dept. of Earth, Environment and Life Sciences (DISTAV), University of Genova, Genova, Italy.
PANDORA (Probing safety of nano-objects by defining immune responses of environmental
organisms) is a European Training Network (ETN) funded in the framework of H2020 Marie
Skłodowska- Curie ITN programme. The PANDORA network aims at the education of
promising young scientists who will learn how to assess the impact of engineered nano-
objects (nanoparticles, NP) on the immune and defensive responses of organisms in the
environment. PANDORA will tackle the issue of global immunological nanosafety by
comparing the effects of a selected number of NP of wide application on the immune
response of several earth and marine organisms in parallel to human. The highly conserved
system of innate immunity/stress response/inflammation will be the focus of PANDORA, as
this would allow us to identify common reactivity across immune defence evolution. The
research activities implemented in PANDORA have the following objectives: 1. To identify
immunological mechanisms triggered by nano-objects, and predictive markers of risk vs.
safety; 2. To do so by a collaborative cross-species comparison, from plants to human, of
innate immune defence capacity, using selected, industrially-relevant NP; 3. To design
predictive in vitro assays to measure the immuno-risk of NP to the environment and human
health, as new approaches to industrial and environmental nanosafety testing.
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 53 Oral Communication - Session III
A biodistribution study of two differently shaped plant virus nanoparticles
reveals new peculiar traits
C. Lico1, P. Giardullo
2,3, M. Mancuso
4, E. Benvenuto
1, L. Santi
5, S. Baschieri
1,*
1Laboratory of Biotechnology, ENEA, Via Anguillarese 301, Rome, Italy.
2Department of Radiation Physics, Guglielmo Marconi University, Rome, Italy.
3Department of Sciences, University of Roma Tre, Rome, Italy.
4Laboratory of Biomedical Technologies, ENEA, Via Anguillarese 301, Rome, Italy.
5Department of Agricultural and Forestry Sciences, University of Tuscia, Via San Camillo de Lellis snc, 01100,
Viterbo, Italy.
Self-assembling plant virus nanoparticles (pVNPs) have started to be explored as nanometre-
sized objects of interest for application in biomedicine. Plant VNPs may be ideal in terms of
biocompatibility and biodegradability and are attractive because of the wide diversity of
symmetries and dimensions, easy chemical/biological engineering, easy and rapid production
in plants. Recently, we defined that icosahedral Tomato bushy stunt virus (TBSV) and
filamentous Potato virus X (PVX) are neither toxic nor teratogenic. To further contribute to
understand the behaviour of these nanoparticles in vivo, we report here the results of an
interdisciplinary study aimed to define for the first time the biodistribution of unlabelled,
unpegylated, underivatized TBSV and PVX through the use of reliable detecting antibodies.
These data add new important information about the in vivo behaviour of these nano-objects
and demonstrate that these pVNPs are intrinsically endowed with different and peculiar
properties, in terms of tissue and organ localization, persistence in the bloodstream,
association to cell membrane. The structural and behavioural differences between the two
viruses, will allow to select the most appropriate nano-scaffold, as a function of the foreseen
application (e.g. drug targeting and delivery, imaging, development of new diagnostic tools).
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 54 Oral Communication - Session III
Physiological and molecular response Cucurbita pepo exposed to ENM
binary combinations
L. Pagano1,2,3,*
, F. Pasquali1, S. Majumdar
3, R. De la Torre-Roche
3, N. Zuverza-Mena
3, M. Villani
4, A.
Zappettini4, R.E. Marra
3, S.M. Isch
5, M. Marmiroli
1, E. Maestri
1, O.P. Dhankher
2, J.C. White
3, N.
Marmiroli3
1Dept. Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parma, Italy.
2Stockbridge School of Agriculture, University of Massachusetts, Amherst, MA, USA.
3The Connecticut Agricultural Experiment Station, New Haven, CT, USA.
4IMEM-CNR, Parma, Italy.
5Dr. Katherine A. Kelley State Public Health Laboratory, Rocky Hill, CT, USA.
Although engineered nanomaterial (ENM) uptake, transport and response mechanisms in
plants have received increased attention in the recent years, many questions regarding ENM
risks to the environment and to food safety remain unanswered. The impact of ENM
interactions with co-existing organic and inorganic contaminants, including secondary ENMs,
remains poorly understood. The physiological and molecular response of zucchini (Cucurbita
pepo L.) under conditions of nanomaterial combined treatments (NMCT) with binary
combinations of nanoparticle (NP) cerium oxide (CeO2), lanthanum oxide (La2O3), copper
oxide (CuO), zinc oxide (ZnO) and cadmium sulfide quantum dots (CdS QDs) were tested
and compared with respective individual (NMIT) and bulk material (BMT) treatments. ICP-
MS results within specific tissues upon exposure to NMCT or NMIT conditions demonstrated
that metal content varied significantly upon co-contaminant exposure, including instances of
antagonistic effects: La uptake was significantly decreased upon CeO2 NP co-exposure
whereas La2O3 NP caused a complete deregulation of Cu uptake upon CuO NP co-exposure.
Expression analysis of specific genes previously shown to be responsive to ENM exposure
confirmed the involvement of the chloroplast in plant response: ORF31, a chloroplastic
electron carrier down-regulated in all treatments, showed potential as a biomarker of
exposure/effect. Principal component analysis (PCA) on plant physiological and molecular
response provided insight into the nature of phytotoxicity under NMIT and NMCT exposure.
This systematic approach is highly useful for characterizing the risk associated with ENMs by
providing a mechanistic interpretation and a holistic perspective for more complex systems of
contamination.
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 55 Oral Communication - Session III
Phytonanotechnology: a new horizon to food security
L. Marchiol1
1University of Udine, Via delleScienze 206, Udine, Italy.
Nanomaterials have rapidly gained importance in many fields of science and technology due
to their unique properties. Agriculture field is also looking towards this newly growing
technology with great hope for future sustainability. Although the use of nanotechnology in
agriculture (phytonanotechnology) is relatively a new idea as compared to the other sectors
(e.g. industry, energy, aerospace, medicine), it has been considered to be an important tool for
enhancing resistant against stress as well as enhancing the crop yield. In particular,
phytonanotechnology has the potential to alter conventional plant production systems,
allowing for the controlled release of agrochemicals and target-specific delivery of
biomolecules. However, the development of effective, safe and sustainable
phytonanotechnology strategies requires intense research and development.
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 56 Oral Communication - Session IV
Approaches for nanomaterial safety
I. De Angelis
Health and Environment Department, Istituto Superiore di Sanità, Rome, Italy.
Despite the increasing use of nanomaterials (NMs) in consumer products and in biomedical
fields, there is a scientific uncertainty about the safety of nanomaterials for both human health
and environment.
NMs have peculiar physico-chemical properties that make them suitable for innovative
applications but, on the other hand, they may be responsible of interaction with living system
that are unpredictable solely on the base of the chemical structure.
Although an increasing number of reports in the literature have shown adverse effects of
NMs, data are still contradictory and/or inconclusive. This is mostly related to bias in the
conduction of experiments as differences in sample preparation, NMs quantification and
characterization, dosimetry and stability of test solutions. Moreover, many parameters can
vary from nanoform to nanoform or even within the same nanoforms; therefore a case-by-case
strategy in NMs Risk Assessment (RA) is often suggested. For this reason, in NMs RA
emphasis is now placed on approaches for tiered testing schemes, read-across and grouping,
as well as modelling approaches and in silico methods.
It is generally accepted that methods and tools for traditional chemicals are in principle
suitable for NMs hazard assessment but their unique properties require that protocols (e.g. test
guidelines and guidance) are adapted when applied to NMs, as well as new ones developed to
address specific regulatory endpoints.
On this respect, in vitro tests are especially relevant in the early phase of NMs evaluation for
screening purposes and for identification of potential toxicity mechanisms as, for example,
genotoxicity.
Since the 7th
Framework Programme, European Commission and National Governments
have considered as priority research area the investigation on NMs safety and a large amounts
of money has been allocated on this topic. About 50 research projects were funded with the
aim of increasing knowledge on the potential hazard of NMs and providing answers to
regulatory requirements.
Within national and European research projects, the Italian National Institute of Health
(Istituto Superiore di Sanità) has developed competences and specific tools for NM hazard
assessment, taking advance of a multidisciplinary approaches and competences becoming an
important national point of reference on this topic.
In conclusion, the way forward in NMs RA is to develop robust, fast and cost-effective
methods appropriate for regulatory purposes, in order to keep pace between technological
innovations and safe use.
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 57 Oral Communication - Session IV
Recovery of cardiac impulse propagation in ischemic rat heart via silicon
carbide conductive nanowires (SiC-NW)
M. Miragoli
Department of Medicine and Surgery,University of Parma
Structural remodeling of the heart as occurring in the context of myocardial infarction (MI) is
the primary cause of arrhythmias and sudden death. Apart from changes in the size and
number of cardiomyocytes (CMs), the histopathology of MI is characterized by the
appearance of myofibroblasts (MFBs). MFBs persist locally for years and continue to secrete
collagen under humoral control. The resulting electrically insulating collagenous septa
contribute to arrhythmia by forming a non-uniform substrate for impulse propagation, which
causes activation wavefronts to take irregular (zigzag) pathways, resulting in slow conduction
and conduction blocks. We found that MFBs directly depolarize coupled CMs and induce
ectopic activity. Treatments for reducing post-MI arrhythmias show limited efficiency,
including recent approaches in regenerating MI tissue by stem cells or genetically
reprogrammed MFBs. Hence, other strategies have to be explored.
We started from HL-1 cardiac cells line cultured from 3 days together with semi-conductive
silicon carbide nanowires (SiC-Nw), in a controlled bioelectric and biomechanics
environment. Optogenetic and optical mapping of impulse propagation showed that SiC-NW
supported electric propagation and perform electrical coupling among distant cardiac cells by
synchronizing the spontaneous bioelectrical activities over distances. In-vivo preliminary data
indicate an anti-arrhythmogenic effect of SiC-NWs once injected into induced-ischemic
regions of the rat hearts. In the future, the predicted intervention strategies will be transferred
and validated infarcted animal where the electrical milieu of MFs can be manipulated by
nanotechnologies. On a very simple conceptual basis, showing that a possible SiC-NWs-
conductive cardiac patches are capable of re-establishing electrical communication in
infarcted areas would offer a new strategy for increasing post-MI life expectance
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 58 Oral Communication - Session IV
3D printing of novel PCL/bioactive glass scaffolds for bone tissue
regeneration: nanoindentation and finite element analyses
G. Marchiori1*
, M. Berni1, M. Petretta
1, C. Gualandi
2, M. Boi
1, D. Bellucci
3, C. Garavelli, M.L.
Focarete2, V. Cannillo
3, B. Grigolo
1, M. Bianchi
1
1Rizzoli Orthopedic Institute, Bologna, Italy.
2University of Bologna, Italy.
3University of Modena and Reggio Emilia, Italy.
Introduction
3D scaffolds composed by a polymeric matrix loaded with an inorganic phase are of
increasing interest in bone regeneration [1]. The objective of this study was to perform a
systematic study of the role of composition and 3D architecture on the mechanical
performances of PCL/Bioactive glass scaffolds.
Methods
Composites were obtained by mixing PCL pellets and bioactive glass powders with
innovative formulation (BGMIX_Mg [2], size < 25 µm) in a different wt.% ratio and
characterized by nano-indentation (NHT2, CSM Instruments SA) before and after plotted by
Fused deposition modelling (FDM) with a 3D Discovery printer (RegenHU, Switzerland), in
order to reveal potential effects of the printing on the material properties. Post-printing
nanoindentation outputs were then used as inputs for Finite Element Analysis (FEA) together
with geometrical parameters such as fiber and pore size (μm), layer orientation, height and
porosity in order to optimize the scaffold design. By FEA, compression tests on scaffolds
were simulated according to ASTM [3]. Scaffold performances were evaluated in terms of
compressive modulus (GPa), in dependence of architecture and material composition.
Optimized scaffold designs were then proposed for manufacturing and compression testing
[3] in order to reveal their strength.
Results and Discussion
Nanoindentation highlighted a great influence of composition and printing on PCL/Bioactive
glass mechanical properties. FEA showed that material composition had more influence on
compressive modulus than scaffold geometry, vice versa on strain. This could suggest to
choose the most compliant geometry and tune the material composition for reaching the
desired stiffness.
References
1. Poh et al, Acta Biomater, 30:319-333, 2016.
2. Bellucci et al, Mater Sci Eng C, 72:566–575, 2017.
3. ASTM D1621
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 59 Oral Communication - Session IV
Quantitative determination of dye doped silica nanoparticles in cancer cells
A. Procopio1, A. Sargenti
1, C. Cappadone
1, G. Farruggia
1, N. Zaccheroni
2, E. Rampazzo
2, F.
Palomba2, L. Merolle
3, A. Gianoncelli
4, S.o Iotti
5, E. Malucelli
5*
1Dipartimento di Farmacia e Biotecnologie, Università di Bologna, Via San Donato 19/2, Bologna 40127, Italy.
2Dipartimento di Chimica “G. Ciamician”, Via Selmi 2, 40126, Bologna, Italy.
3 ASMN-IRCCS, Dipartimento di Medicina Trasfusionale, Reggio Emilia, Italy.
4Elettra - Sincrotrone Trieste, 34149 Basovizza, Trieste, Italy.
4 Dipartimento di Farmacia e Biotecnologie, Via San Donato 15, University of Bologna, Bologna 40127, Italy
Dye doped silica nanoparticles (SiNPs) constitute very effective nanoplatfoms to obtain
efficient luminescent, stable, biocompatible and targeted agents for biomedical applications.
In this study, we have used silica-PEG core-shell nanoparticles, obtained by a one-pot
synthesis, based on preparation of Pluronic F127 micelles in water. The obtained
monodisperse aqueous suspension of nanoparticles (25 nm of diameter) shows high stability
also in the presence of proteins, offering a valuable advantage compared to other
nanostructures.
These SiNPs did not show toxicity up to 800 nM, and neither ROS induction, the mostly
recognized mechanism of cell damage for SiNPs.
To quantitatively determine the Si concentration and distribution in LoVo (Human colon
adenocarcinoma) cells loaded with dye SiNPs, we combined X-Ray Fluorescence Microscopy
(XRFM) and Scanning Transmission X-ray Microscopy (STXM). The Si molar concentration
requires knowing the volume of the illuminated area, from which the fluorescence intensity
originated. The volume has been calculated by the STXM considering an intracellular
constant density of 1.2 g/cm3.
These results showed the internalization of the SiNPs, mainly deploying in the perinuclear
area (Fig 1). The number of SiNPs present within the cell has been calculated considering a
constant value 15x103 of Si atoms for each SiNPs. In particular, in the cell showed in Figure
1, we estimated a number of 727x103 SiNPs.
The approach of combining XRFM with STXM to achieve the concentration maps of
exogenous nanoparticles in cells is still at its infancy and it represents an important step on the
way of nanoparticles quantitative determination and intracellular compartmentalization.
Figure1. Panel a shows the map of Si fluorescence obtained by XRFM; panel B shows the map of transmission
obtained by STXM; panel c shows the map of Si concentration obtained by combination of XRFM and STXM
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 60 Oral Communication - Session IV
Odorant binding protein-functionalized superparamagnetic nanoparticles
for biomedical applications
N. Riboni1*
, F. Bianchi1, R. Ramoni
2, S. Grolli
2, V. Conti
2, F. Casoli
3, C. de Julián Fernández
3, L.
Nasi3, F. Bisceglie
1, P. Luches
4, M. Careri
1
1Dipartimento di Scienze Chimiche, della Vita e della Sostenibilità Ambientale, Università di Parma, Parco
Area delle Scienze 17/A, 43124 Parma, Italy. 2Dipartimento di Scienze Medico-Veterinarie, Università di Parma, Via del Taglio 10, 43126 Parma, Italy.
3Istituto dei materiali per l'elettronica ed il magnetismo, Parco Area delle Scienze, 37/A, 43124 Parma, Italy.
4Istituto Nanoscienze del CNR, Via Campi 213/a, I-41125 Modena, Italy.
Quorum sensing (QS) is a system of stimuli and responses related to population density of
bacteria: when the secreted signaling molecules (inducers) binds the receptors, the
transcription of certain genes is activated. Odorant binding proteins (OBPs) are
multifunctional scavengers secreted by the nasal epithelia of mammals, characterized by
broad ligand binding specificity towards a large number of natural and synthetic molecules
of different chemical classes1 (Fig. 1). Preliminary investigations proved that OBPs are able
to bind QS molecules like acyl-homoserine lactones and pyocianin.
Being able to be driven towards a specific inflammation zone by using an external magnetic
field, superparamagnetic nanoparticles (MNPs) functionalized with bovine-OBP (bOBP)
are proposed as targeted quorum quenching agents to be used in lung diseases.
A three step synthesis was performed: i) synthesis of the bare MNPs having a diameter of
6.5 ± 1.1 nm; ii) functionalization with
phosphonoundecanoic and
phosphonopropionic acids as linkers; iii)
functionalization with bOBP.
MNPs were characterized by VSM, TEM,
XPS, EDX, IR spectroscopy and by using a
colorimetric assay (BCA Protein Assay Kit).
The bOBP amount on the surface of the
nanoparticles was estimated at 6.0 ± 1.1
mgOBP/g MNP.
With respect to bare MNPs, a good
magnetization was retained after bOBP
functionalization (57 vs 44 emu/g), thus allowing their use for biomedical applications.
1F. Bianchi, G. Basini, S. Grolli, V. Conti, F. Bianchi, F. Grasselli, M. Careri, R. Ramoni, Anal. Bioanal.
Chem. 405 (2013) 1067-1075
Figure 1. bOBP structure
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 61 Oral Communication - Session IV
Growth of Bismuth Titanate into mesoporous silica nanoparticles: a new
safe self-sealing UV filter for cosmetic formulations
G. Zaccariello1,*
, M. Back1, M. Zanello
1, P. Canton
1, E. Cattaruzza
1, P. Riello
1, A. Alimonti
2, A.
Benedetti1
1Università Ca’Foscari di Venezia, Via Torino 155/b, Venezia, Italia. 2Istituto Superiore di Sanità, Viale Regine Elena 299, Roma, Italia.
The use of nanosized inorganic UV filters (i.e. TiO2 and ZnO) in sunscreen formulations is
limited by the high photocatalytic properties that they show under UV irradiation. The
photocatalytic activity could induce the degradation and a chemical transformation of the
organic molecules in sunscreen formulations. To overcome this problem the commercial
sunscreens contain TiO2/ZnO nanoparticles modified with an external coating. However, the
development of new strategies to simultaneously tail off the photocatalytic activity of the
active phase and enlarge the window of filter’s absorption is still a challenge. In this view, we
propose the use of bismuth titanates BixTiyOz grown into mesoporous silica network of MSN.
The growing process of this new nanosystem is analyzed underlining the key role of the
bismuth ion that, acting as a low-melting point agent for the silica framework, led to a self-
sealing mechanism, by which the active phase is embedded into the silica matrix. The
chemical-physical properties were investigated by means of XRPD, TEM, UV-vis
spectroscopy, N2 physisorption, XPS, and SF-ICP-MS analysis. The photocatalytic activity
was evaluated through photocatalytic tests. The radical suppression of the photocatalytic
activity and the excellent UV shielding properties make the proposed nanosystem a perfect
candidate for the development of the next generation nanomaterials for cosmetic and skin care
formulations.
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 62 Oral Communication - Session IV
microRNAs transcriptional regulation in human cells exposed to CdS QDs
L. Paesano1,*
, M. Bianchi2, G. Guglielmi
1, M. Villani
3, O. Bussolati
2, M. Marmiroli
1
1Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parma, Italy.
2Department of Medicine and Surgery, University of Parma, Parma, Italy.
3IMEM-CNR, Parco Area delle Scienze 37/A, 43124 Parma, Italy.
The miRNAs are small non-coding RNAs that regulate gene expression at a post-
transcriptional level and exhibit a broad range of biological functions in a myriad of cellular
processes. Thus, the expression of miRNAs is under tight regulation and the changes in their
expression profile can alter either globally or selectively protein output and influence key
biological processes leading to disease development1. Several studies on the involvement of
miRNA were reported within pathological contexts, such as mitochondrial dysfunction2, HIV
infection3, and cystic fibrosis
4. Several studies revealed that miRNAs are regulated in
response to the cytotoxicity of nanomaterials2,5
. Here we demonstrate that both miRNA and
mRNA expression profiles were modified during exposure to quantum dots (QDs).
In a previous in vitro study on the toxicity of CdS QDs, we utilized HepG2 cells to find out
biomarkers of exposure to CdS QDs6. In a cognate study, we followed the effects in HepG2
cell lines of CdS QDs and Cd ions on microRNA levels and mRNA expression profiles.
Results showed that only a small number miRNAs (66) were differentially expressed during
CdS QDs exposure, whereas 131 miRNAs were altered in their expression by Cd ions. We
compared mRNA and miRNA regulation and performed a network analysis using the web-
resource DIANA-mirPath to provide a comprehensive understanding of CdS QD toxicity. The
ultimate goal will be to identify the role of miRNAs in the toxicity mechanism, so as to
identify specific miRNAs as reliable biomarkers of CdS QDs exposure.
1 Genovesi LA, Anderson D, Carter KW, Giles KM, Dallas PB, 2012. Identification of suitable endogenous
control genes for microRNA expression profiling of childhood medulloblastoma and human neural stem cells.
BMC Research Notes, 5:507 2 Huang Y, Lu X, Qu Y, Yang Y, Wu S, 2015. MicroRNA sequencing and molecular mechanisms analysis of
the effects of gold nanoparticles on human dermal fibroblasts. Biomaterials 37:13 3 Bignami F, Pilotti E, Bertoncelli L, Ronzi P, Gulli M, Marmiroli N, Magnani G, Pinti M, Lopalco L, Mussini
C, Ruotolo R, Galli M, Cossarizza A, Casoli C, 2012. Stable changes in CD4+ T lymphocyte miRNA expression
after exposure to HIV-1. Blood 119:6259 4 Montanini L, Smerieri A, Gulli M, Cirillo F, Pisi G, Sartori C, Amarri S, Bernasconi S, Marmiroli N, Street
ME, 2016. miR-146a, miR-155, miR-370 and miR-708 are CFTR-dependent, predicted FOXO1 regulators and
change at onset of CFDs. J Clin Endocrinol Metab 101:4955 5 Eom HJ, Chatterrjee N, Lee J, Choi J, 2014. Integrated mRNA and micro RNA profiling reveals epigenetic
mechanism of differential sensitivity of Jurkat T cells to AgNPs and Ag ions. Toxicology Letters 229:311 6 Paesano L, Perotti A, Buschini A, Carubbi C, Marmiroli M, Maestri E, Iannotta S, Marmiroli N, 2016. Markers
for toxicity to HepG2 exposed to cadmium sulphide quantum dots; damage to mitochondria. Toxicology 374:18
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 64 Poster Communication - Session A
Effect of the hollow topology on the local spin dynamics in Iron Oxide
MNPs
M. Basini1, D. Peddis
2 and A. Lascialfari
1
1Dipartimento di Fisica and INSTM, Universita' degli Studi di Milano, 20133 Milano, Italy.
2ISM-CNR, Area della Ricerca Roma 1, 00016 Monterotondo Scalo (RM), Italy.
This research has been developed within the framework of a general research concerning novel
systems based on magnetic nanoparticles with different spin topologies that present, consequently,
different static and dynamic magnetic properties. In particular the experimental investigation and
data analysis were focused on the effect of the hollow core spins topology. Nanoparticles with a
hollow magnetic core (HNP) of variable dimensions constituted of maghemite (-Fe2O3) and/or
magnetite (Fe3O4) have been studied, while nanoparticles having a full core (FNP) and the same
magnetic phase, were used as reference systems with superparamagnetic monodomain
conventional behaviour.
The static answer of the systems has been evaluated as a function of field and as a function of
temperature by means of DC magnetic measurements.
The characterization of the local spin dynamics have been performed as a function of frequency
by means of nuclear magnetic resonance dispersion profiles (NMR-D) and as a function of
temperature by means of muon spin resonance (MuSR) experiments.
The NMR-D of the hollow samples revealed the presence of a paramagnetic-like contribution
at high fields (>30MHz), which was ascribed to disordered surface spins, not visible in the
NMR-D of the full samples. To interpret the longitudinal relaxation experimental data a novel
phenomenological model was proposed.
The time-decay constant of the muon asymmetry was monitored as a function of temperature
and revealed a critical temperature (T* similar for HNP and FNP of comparable volume)
below which the electronic spins are blocked with respect to the frequency window of the
probes (muon’s Larmor frequency). At very low temperature, the presence of an anomaly,
much more evident in HNPs, was observed and ascribed to the surface spins. Additionally, for
the first time in a MuSR experiment on magnetic nanoparticles the presence of a static local
field (Hloc) was observed when the temperature is decreased below T*, thus indicating a local
magnetic order observable by muons.
[1] A. Cabot, J. Am. Chem. Soc. 129, 10358-10360 (2007)
[2] A. Cabot et al. Phys. Rev. B 79, 094419 (2009)
[3] H. Khurshid et al. Appl. Phys. Lett. 101, 022403 (2012)
[4] H. Kurshid et al. J. Appl. Phys. 115, 17E131 (2014)
[5] H. Khurshid et al. scientific reports 5:15054 (2014)
A.01
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 65 Poster Communication - Session A
Stabilization and thermal conductivity of aqueous nanofluids
M. Cannio*, M. Lassinantti Gualtieri, M. Romagnoli
Department of Engineering “Enzo Ferrari”, University of Modena and Reggio Emilia, Modena 41125, Italy.
Nowadays cooling is one of the most important technical challenges that many different
industries, such as microelectronics, transportation, solid-state lighting, and manufacturing
face and try to solve. Therefore there is an urgent need for new and innovative coolants with
improved performance: ‘nanofluids’, suspensions of nanoparticles (nominally 1–100 nm in
size) in conventional fluids, have been proposed as a means of meeting these challenges in
order to surpass the performance of heat transfer liquids currently available. In this work,
different nanoparticles_NPs (in particular magnetite, titania, silica, Ag/Pt), with controlled
morphology and size, commercial or obtained by microwave-assisted hydrothermal synthesis
and/or electrochemical approach, were investigated. Since the agglomerate sizes can
significantly impact the thermophysical properties of nanofluids and lead to a different heat
transfer performance, the preparation of stable suspensions is of great importance. In
particular, various parameters affecting stability of aqueous nanofluids suspension such as
dispersant addition, sonication time and pH were considered. The stability of the nanofluids
was studied by zeta potential, particle size distribution and UV–vis spectroscopy method
(following the investigated suspensions during a period of time). Finally, once identified the
most stable suspensions, their thermal properties were measured to evaluate the possible
application as heat transfer fluid. The preliminary results indicate a significant enhancement
of NPs water based nanofluid thermal conductivity with respect to water (up to 25%) and
conventional water based fluid.
A.02
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 66 Poster Communication - Session A
Solid state NMR spectroscopy and nuclear spin relaxometry for the
investigation of nanomaterials
E. Carignani1,2,*
, S. Borsacchi3,2
, F. Martini4,2
, L. Calucci3,2
, C. Forte3, M. Geppi
4,2
1Consiglio Nazionale delle Ricerche- Istituto per i Processi Chimico-Fisici (CNR-IPCF),
Via G. Moruzzi 1, 56124 Pisa, Italy. 2 Consorzio Interuniversitario Nazionale per la Scienza e Tecnologia dei Materiali (INSTM),
Via G. Giusti 9, 50121 Firenze, Italy. 3Consiglio Nazionale delle Ricerche - Istituto di Chimica dei Composti OrganoMetallici (CNR-ICCOM),
Via G. Moruzzi 1, 56124 Pisa, Italy. 4Dipartimento di Chimica e Chimica Industriale, Università di Pisa,
Via G. Moruzzi 13, 56124 Pisa, Italy.
Solid state NMR is a very powerful technique for the investigation of a large variety of
multicomponent nanostructured materials [1-3]. Thanks to its wide applicability and
versatility has gained a crucial role among solid state techniques. On the other hand, nuclear
spin relaxometry is especially suited for the investigation of properties of nanostructured
porous materials and contrast agents [4, 5].
In this contribution some applications, especially concerning the pharmaceutical and
biomedical fields, will be shown, taking examples from studies carried out in our research
group.
In particular, it will be highlighted how solid state NMR can give information on physical
and/or chemical interactions occurring at the interface between the components, on the
structural and dynamic behaviour of each component, and on the dimensions and dispersion
of the corresponding domains. Moreover, examples will be given on the possibility to
investigate in detail the state of water in porous materials and the mechanism of contrast
enhancement by nuclear spin relaxometry.
References
[1] A. Marchetti, J. Chen, Z. Pang, S. Li, D. Ling, F. Deng, X. Kong Adv. Mater., 2017, 29, 1605895.
[2] L. A. Straasø, Q. Saleem, M. R. Hansen Ann. Rep. NMR Spectrosc., 2016, 88, 307-383.
[3] M. Geppi, S. Borsacchi, G. Mollica, C.A. Veracini Appl. Spectrosc., Rev. 2009, 44, 1-89.
[4] R. Kimmich and E. Anoardo Progr. Nucl. Magn. Reson. Spectrosc., 2004, 44, 257-320.
[5] C. J. Meledandri, D. F. Brougham Anal. Methods, 2012, 4, 331-341.
A.03
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 67 Poster Communication - Session A
Synthesis and investigation of croconates as smart organic coating for nobel
metals nanoparticles
B. Cogliati1,*
, A. Arduini1, A. Secchi
1, L. Serravalli
2, G. Trevisi
2 and P. Frigeri
2
1Department of Chemistry, Life Sciences an Enviromental Sustainability – COFI unit – Parco area delle scienze
17/A. I-43124 Parma, Italy. 2 Istituto Materiali per Elettronica e Magnetismo IMEM-CNR, MBE Group, Parco Area delle Scienze 37/A –
43124 Parma, Italy.
Croconic acid is a cyclic organic molecule, belonging to a particular family of
compounds called oxo-carbon acids. This molecule properly functionalized exhibit an
absorption in NIR region and this property can be exploited in the design of NIR-harvesting
materials obtained with a hybridization of a nano-material, characterized by a NIR absorption,
with this organic molecule.
The purpose of this research is to combine a particular type of gold nanoparticles,
called nanorods (AuNRs), with a specific aspect ratio (AR) in order to have an absorption in
NIR region (900-1100 nm), with a croconic acid. This latter must be properly functionalized
with an alkyl spacer (for example thiol-ending) in order to allow the anchoring to the AuNRs.
O
O
O
A1
A2n X
X = -SH, -COOH, -NH2
anchoringgroup
NPs
The difficulty is to detect a synthetic pathway for the synthesis of the croconic acid
and here two strategies are proposed (Scheme 1), as reported in literature [1], [2]
. Gold nanorods
are synthetized with a Seed-Mediated Growth method [3]
.
Scheme 1
[1] Fatiadi, A.; Isbell, H.; Sager, W. Journal of Research of the national Bureau of Standards- A.Physics and
Chemistry. 1962, 67A. 153-162.
[2] FUJI PHOTO FILM CO., LTD. - Patent: EP1707552 A1, 2006.
[3] Nikoobakht, B; El-Sayed, M.A. Chem. Mater. 2002, 15, 1957-1962.
A.04
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 68 Poster Communication - Session A
ZnO nanorod-based piezoelectric stress sensor embedded within carbon
fiber composite
M. Culiolo1,*
, M. Villani1, D. Delmonte
1, D. Calestani
1, N. Coppedè
1, M. Solzi
2,
L. Marchini3, R. Bercella
3, A. Zappettini
1, T.Y. Kim
4, S.W. Kim
4,5
1 IMEM-CNR, Parco Area delle Scienze 37/A, Parma, Italy.
2 Dipartimento di Fisica e CNISM, Università di Parma, Via G.P. Usberti 7/A, Parma, Italy.
3 Bercella s.r.l., Via Enzo Ferrari 10, Varano de’ Melegari (PR), Italy.
4 SKKU Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University (SKKU), Suwon 440-746,
Republic of Korea. 5 School of Advanced Materials Science and Engineering (AMSE), Sungkyunkwan University (SKKU), Suwon
440-746, Republic of Korea.
Carbon fiber composites (CFC) are a fundamental class of materials in applications where
both high mechanical resistance and light weight are requested (automotive, civil
engineering, aerospace, etc.). Thanks to the latter feature, the use of these materials in
transportation leads to significant reduction of CO2, NOx and noise. Mentioning the aviation
field, an aircraft composed of 53% CFC by weight such as Airbus 350XWB can save up to
25% fuel. A life-cycle CO2 emissions analysis shows a reduction up to 2700 tons of CO2
per aircraft every year (Boeing 787 Dreamliner, 50% CFC by weight).
Nevertheless, due to their intrinsic structure strongly dependent on carbon fiber (CF) fabric
and resin arrangement, a precise predictive deformation and failure behavior is hard to
model, which is one of the main causes limiting the use of these materials in aerospace
technologies. Hence, on account of the very heavy loads the CFC structures are subject to,
it is important to carry out real-time monitoring of deformations and vibrations.
Nowadays’ stress sensors in CFC, mainly based on piezoelectric PZT and optical fibers
(Fiber Bragg Grating, FBG), present some drawback such as large size (compared to CF’s)
and weight addition. The use of zinc oxide (ZnO) piezoelectric nanostructures can
overcome these issues and lead to a real-time stress sensor completely embedded within
CFC.
In this work low cost, low temperature and low environmental impact synthesis of ZnO
nanorods on CF is carried out, as well as piezoelectric characterization of such micro-
composite sensor.
A.05
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 69 Poster Communication - Session A
Synthesis of water dispersible and catalytically active gold-decorated cobalt
ferrite nanoparticles
A. M. Ferretti1,*, A. Silvestri1,2, M. Marelli1, V.Pifferi2, L.Falciola2, A. Ponti1, and L. Polito1
1CNR – ISTM, Nanotechnology Lab., Via G. Fantoli 16/15, 20138 Milan, Italy.
2University of Milan, Department of Chemistry, via C. Golgi 19, 20133 Milan, Italy.
One of the most compelling goals in the field of the nanotechnologies is the design and
synthesis of hetero-structured nanoparticles containing different types of materials. This kind
of structures is able to combine in a single entity the physical and chemical properties of the
composing materials. Here we report a new strategy for the synthesis of cobalt ferrite (CoxFe3-
xO4) decorated with ultrasmall ( d= 2-3 nm) gold nanoparticles (NPs).The synthetic procedure
exploits double role of the of 2,3-meso-dimercapto succinic acid (DMSA): as phase transfer
for cobalt ferrite NPs from organic to aqueous media, and as promoter of the nucleation of
gold clusters only in proximity of the magnetic nanoparticle surfaces, thanks to its reducing
properties. We present also a complete morphological (TEM, STEM) and chemical
characterization, based on microanalysis methods (EFTEM, EELS and EDX). The new
obtained nano-hertostructure combines the
magnetic properties of cobalt ferrite with the
catalytic properties of small noble metal
clusters in a single entity (figure 1). So we
investigate the catalytic activity of
CoxFe3-xO4-Au NPs using a model reaction, that
is the reduction of 4-nitrophenol (4-NP) to 4-
aminophenol (4-AP), using NaBH4 as reducing
agent. The catalyst has been magnetically
recovered up to 5 times, maintaining excellent
kobs over the cycles
DOI: 10.1021/acs.langmuir.6b01266
Figure 1 STEM image of CoxFe3-xO4-DMSA-Au
NPs obtained using the optimized experimental
conditions
A.06
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 70 Poster Communication - Session A
Ultrastructural analysis of dental ceramic surfaceprocessed by a 1070 nm
fiber laser
C. Fornaini1, F. Poli
1,*, E. Merigo
2, S. Selleri
1, A. Cucinotta
1
1Department of Engineering and Architecture, Parco Area delle Scienze 181/A, 43124 – Parma, Italy.
2Micoralis Laboratory, Faculty of Dentistry, University of Nice, 24 Avenue des Diables Bleus, 06357 Nice,
France.
Lithium di-silicate ceramics offer high accuracy when used in prosthetic dentistry. Their
bonding, using different resins, is highly dependent on micro-mechanical interlocking and
adhesive chemical bonding. Ceramic surface treatment increases the contact area with the
tooth structure, creating micro-porosities and enhancing the potential for mechanical retention
of the cement. Different surface treatment methods have been proposed in literature. Non-
destructive techniques to treat inert ceramics and modify their mechanical and chemical
characteristics help to produce an activated surface. Performance investigation of high
strength ceramics when their surface is modified for chemical and mechanical bonding is
required.
The aim of this study is to investigate the possibility of using fiber lasers for surface treatment
of Lithium di-silicate ceramics, thus improving their mechanical and chemical properties.
Samples were irradiated by a 1070 nm pulsed fiber laser (AREX 20) provided by Datalogic,
Italy, with different parameters. Surface modifications were analyzed by optical microscope,
SEM and EDS (Energy Dispersive X-ray Spectroscopy). The observation of the irradiated
surface shows a roughness increase with small areas of melting and carbonization. EDS
analysis revealed that, by using proper laser parameters, no evident differences between laser-
processed samples and controls are obtained. These preliminary results have shown that fiber
laser irradiation can be considered an effective tool to increase the Lithium di-silicate
ceramics adhesion.
A.07
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 71 Poster Communication - Session A
Plasma processing of oxide-based nanomaterials for sensing and energy
applications
A. Gasparotto1,*
, G. Carraro1, C. Maccato
1, D. Barreca
2
1Department of Chemistry, Padova University and INSTM, 35131 Padova, Italy.
2CNR-ICMATE and INSTM, Department of Chemistry, Padova University, 35131 Padova, Italy.
Cold plasmas are versatile, soft tools for the synthesis, modification and engineering of
functional materials. In the field of inorganic nanosystems, plasma-assisted routes such as
sputtering and plasma enhanced-chemical vapor deposition (PE-CVD) offer a high control
over topological, structural and compositional material features, impacting, in turn, a broad
variety of technological applications.
In this contribution, attention will be focused on selected case studies demonstrating the high
potential of plasma processing in the tailored fabrication of single-phase and composite
Fe2O3-based functional nanostructures. Representative examples will include:
i) the modification of ε-Fe2O3 nanorods by sputtering of Au nanoparticles under mild
conditions, allowing to preserve the 1D iron oxide morphology and to tailor gold content and
distribution as a function of sputtering time. Gas sensing tests aimed at NO2 detection
evidenced that Au introduction resulted in enhanced performances thanks to the intimate
Au/ε-Fe2O3 interfacial contact and the occurrence of Schottky junction effects;
ii) the synthesis of Pt/α-Fe2O3 nanocomposites by a hybrid synthetic route, consisting in the
PE-CVD of iron(III) oxide followed by platinum sputtering and eventual annealing in air.
Material characteristics such as Pt oxidation state and hematite nano-organization strongly
affected the system photoelectrochemical performances in sunlight-assisted water splitting;
iii) α-Fe2O3-TiO2-Au nanocomposites, fabricated by a three-step plasma-assisted strategy, and
tested in the solar-driven H2 generation via photoreforming of ethanol aqueous solutions.
Compared to bare hematite, Fe2O3-TiO2-Au photocatalysts displayed an improved functional
behavior, mainly related to an enhanced interfacial separation of photogenerated charge
carriers.
A.08
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 72 Poster Communication - Session A
Epitaxially grown NiMnGa thin films: the impact of growth conditions on
microstructure and magnetic configuration
M. Takhsha Ghahfarokhi1,*
, F. Casoli1, S. Fabbrici
1,2, R. Cabassi
1, F. Albertini
1
1 IMEM-CNR, Parco Area delle Scienze 37A – 43124, Parma, Italy. 2 MIST E-R, Via P. Gobetti, 101 – 40129, Bologna, Italy.
Heusler compounds have constantly shown novel emerging properties due to the giant effects
(e.g. magnetoelastic, magnetocaloric and barocaloric) driven by external elements (e.g.
magnetic field, temperature and stress).
Compared to the bulk materials, thin films offer further possibility to be applied in
micro/nanosystems and could open a new window to novel-concept nanoactuators, valves,
memories, energy harvesters and solid-state microrefrigerators.
Several studies are being conducted to a better understanding of the mechanisms that would
allow the microstructural control by playing with thickness and epitaxial constraints given by
suitable substrates. The achievement of a fine control of microstructure and magnetic
configuration at the different length scales is of particular importance for the optimization of
shape memory effect and magnetic field induced strain applications, where twin configuration
plays a major role [1].
We have applied RF sputtering technique to epitaxially grow Ni-Mn-Ga films on MgO (100)
with different thicknesses, growth temperatures and deposition rates. 10-800 nm Ni-Mn-Ga
were directly grown on the substrate surface at the growth temperatures from 200 to 400 °C.
The different deposition rates were achieved by changing the applied voltage to the target and
the argon pressure. Microstructures of the thin films were measured using AFM and XRD.
Magnetic configuration of the samples, martensitic transformation and Curie temperature
were studied by MFM, AGFM and SQUID techniques. Results indicate a correlation between
the microstructures of the samples prepared in different growth conditions and their magnetic
features.
[1] Ranzieri, Paolo, et al. Advanced Materials 27.32 (2015): 4760-4766.
A.09
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 73 Poster Communication - Session A
The potentiality of carbon nanostructures for hydrogen storage
G. Magnani1,*
, D. Pontiroli1, M. Gaboardi
1,2, C. Milanese
3, G. Bertoni
4, A. Malcevschi
5,
K.F. Aguey-Zinsou6, M. Riccò
1
1 CNL, DSMFI, University of Parma, Parco Area delle Scienze 7/A, 43124 Parma, Italy.
2 ISIS Facility, Rutherford Appleton Laboratory, Chilton, Didcot, Oxfordshire OX11 0QX, United Kingdom.
3 Pavia Hydrogen Lab, University of Pavia, Viale Taramelli 16, 27100 Pavia, Italy.
4 IMEM-CNR, Parco Area delle Scienze 37/A, 43124 Parma, Italy.
5 DSCVSA, University of Parma, Parco Area delle Scienze 11/A, 43124 Parma, Italy.
6 Materials
Energy Research Laboratory (MERLin), School of Chemical Engineering, The University of New
South Wales Australia, NSW 2052, Sydney, Australia.
This study represents a comprehensive approach to the use of carbon nanostructures for
hydrogen storage, as developed in the Carbon Nanostructures Laboratory group in Parma
University. It has to be placed in the context of a growing demand of renewable fuels such as
hydrogen, identified as a possible replacement to the current gasoline for automotive
application. In this domain, considerable interest is stimulated by the carbon nanostructures
for their relatively low weight and the possibility to modify the interaction energy with the
hydrogen molecule. In particular it is well known that the fundamental physical and chemical
properties of materials can change drastically when entering the nanosize regime so one can
expect important changes in hydrogen sorption properties such as improved kinetics and
reversibility and possibly a change in thermodynamics.
This work analyzes several different approaches in the use of carbon nanostructures materials,
in particular fullerenes, graphenes and biochars, for solid state hydrogen storage applications.
The principal aims of this study have been:
Synthesis, optimization and study of the hydrogen properties of metal clusters intercalated
fullerides, transition metals decorated graphenes, biochars.
Study of the interactions between hydrogen and carbon and mechanisms involved in the
hydrogen sorption and desorption process.
Optimization of nanoparticles dimension and investigation on the role of the size in the
hydrogen storage applications.
Study of the use of metal decorated graphene as a substrate to promote the formation of
nanosized MgH2, investigating the thermodynamic properties in the hydrogen storage field
of the hydride.
A.10
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 74 Poster Communication - Session A
Nano-engineered smart filters: towards induced release
V. Ricci
1,*, S. Erokhina
1, P. D’Angelo
1, V. Erokhin
1, S. Iannotta
1
1IMEM-CNR, Parco Area delle Scienze 37/A, Parma, Italia.
The controlled release of drugs represents a great challenge in current nanomedicine. The
triggering of the drug release dynamics and dosage represent both the first step needed for the
design of efficient implantable polymer-based micro reservoirs able to release drugs on
demand, by an external stimuli.
The aim of our work is to develop smart filters for a controlled release using Polyelectrolyte
Multilayers (PEMs) fabricated by layer-by-layer (LbL) deposition. We have used glass fiber
filters with pores of 0.7 µm in size. Of course, such pores cannot be covered by a nm thick
layers. Therefore, before the layer formation, the pores were filled by a sacrificial material (in
our case, CaCO3 particles) that can be easily dissolved afterwards by an acidic solution (HCl).
Using the LbL technique, the as-prepared filters have been covered with an adhesion layer of
PEI, polyethylenimine, subsequently, with 15 bilayers made of alternate monolayers of an
anionic polyelectrolyte, (the poly[sodium 4-styrenesulfonate], PSS), and of a cationic
polyelectrolyte (the poly[allylamine hydrochloride], PAH).
Using the Scanning Electron Microscopy, we have characterized our filters, confirming that it
is possible to plug the pores and deposit a polyelectrolyte multilayer film on the filter.
After the dissolution of the sacrificial material, further changes in pH, allow controlling the
size of film pores, favoring the passage of the desired molecules through the filter.
A.11
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 75 Poster Communication - Session A
Superparamagnetic nanoparticles with enhanced magnetic
properties:synthesis and biocompatible coating
R. Di Corato
1, A. Aloisi
2, R. Rinaldi
1,2*
1 Dipartimento di Matematica e Fisica “Ennio De Giorgi”, Università del Salento, Via Arnesano, Lecce, Italy.
2 CNR Institute for Microelectronics and Microsystem, SP Lecce-Monteroni, Lecce, Italy.
In the emerging field of nanomedicine, superparamagnetic nanoparticles have been used
primarily as contrast agent for MRI and, more recently, as effectors in magnetic hyperthermia
or as carrier in drug delivery. The research on the synthesis of this class of material is mainly
focused on the enhancement of particles properties, assuring an always more convincing
biocompatibility.
We focused on an update of a synthesis of magnetic nanoparticles firstly reported in 2004 by
Colvin’s group and on a novel method for transferring particles in aqueous media. This
preparation was based on the decomposition of a non–usual iron precursor, the iron(III)
oxide-hydroxide. The main advantage of this approach reside in the possibility to obtain
different sizes of magnetic nanoparticles by a single step reaction. We obtained quasi-
spherical particles in a range between 10 and 40 nm, with a progressive deterioration of
morphological control over the threshold of 25 nm. Moreover, we investigated the effects of
an aggressive oxidation step on the magnetic properties of the particles. The complete
transformation of the different magnetic phases in a pure γ-Fe2O3 (maghemite) led to a higher
magnetic susceptibility and to a magnetic saturation close to the references bulk value. The
nanoparticles above described were transferred in water by a novel affinity exchange method,
grafting on the surface non-modified dextran molecules. The particles remained
monodispersed, with an optimal colloidal stability. In addition, a supplementary chitosan
coating was applied on particles, in order to boost the cellular uptake and to increase the
particle loading capacity.
A.12
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 76 Poster Communication - Session A
Carbon nanostructures for high-performance supercapacitors
S. Scaravonati1,*
, D. Pontiroli1, G. Magnani
1, B. Galante
1, B. Musig
1, A. Malcevschi
2, C. Milanese
3, G.
Lucchesi3,4
, G. Bertoni5, M. Riccò
1
1Dipartimento di Scienze Matematiche, Fisiche e Informatiche, Università degli Studi di Parma, Parco Area
delle Scienze 7/A, 43124 Parma, Italy. 2Dipartimento di Scienze Chimiche, della Vita e della Sostenibilità Ambientale, Università degli Studi di Parma,
Parco Area delle Scienze 11/A, 43124 Parma, Italy. 3Dipartimento di Chimica, Università di Pavia, Viale Taramelli, 16, 27100 Pavia, Italy.
4Dipartimento di Chimica, Università di Firenze, Via della Lastruccia 3, 50019 Sesto Fiorentino (FI), Italy.
5Istituto dei Materiali per l’Elettronica ed il Magnetismo, Parco Area delle Scienze 37/A, 43124 Parma, Italy.
Electric double-layer capacitors, also known as supercapacitors (SC), are very promising
electrochemical devices that bridge the gap between conventional capacitors and rechargeable
batteries. SC store electrostatic energy by exploiting the charge separation at the interfaces
between two porous electrodes and a dielectric (Figure 1). The short distance between the
charge layers (few angstroms) and the very high specific surface area (SSA) of electrodes
determine much higher capacitance values than those of conventional capacitors, of the order
of 10-100 F/g. If compared with rechargeable batteries, SC show faster charge and discharge
rate (higher power density), higher lifetime and lower costs, albeit their energy density is still
much lower than that of Li-ion batteries. In order to achieve high performance SC, the
combination between high SSA with a pore size distribution best suiting the electrolyte ions
dimension is needed.
In this work we identified two classes of different carbon nanostructured materials, which
revealed to be rather promising as SC electrodes, thanks to their high specific surface and
hierarchical porosity: 1) graphene related materials produced by the thermal exfoliation of
graphite oxide (TEGO) and 2) chemical activated bio-chars, namely a charcoal obtained from
the pyrolysis or pyro-gasification of biomasses. In particular, the best investigated system
reached an astounding SSA of up to 3000 m2/g, which, with a favorable pore size distribution,
proved to operate as electrodes in SC with specific capacity of up to 100 F/g.
Figure 1: Working mechanism of a SC
A.13
4
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 77 Poster Communication - Session A
Preparation of acrylate-based silver nanocomposite by simultaneous
polymerization–reduction approach via 3D printing technique
G. Taormina1,*
, C. Sciancalepore2, M. Messori
3, F. Bondioli
1
1Department of Engineering and Architecture, University of Parma, Parco Area delle Scienze 181/A,
43124, Parma, Italy. 2INSTM, Research Unit of Parma, Department of Engineering and Architecture, University of Parma, Parco
Area delle Scienze 181/A, 43124, Parma, Italy. 3Department of Engineering “Enzo Ferrari”, University of Modena and Reggio Emilia, Via P. Vivarelli 10/1,
41125, Modena, Italy.
Three-dimensional (3D) printing has drawn tremendous attention with its potential
applications in various fieldsi. The integration of nanotechnology into 3D printing offers huge
opportunities for the manufacturing of 3D engineered materials exhibiting optimized
properties
The addition of nanofillers affects the printing process: solution viscosity, light penetration
depth and nanoparticles dispersion and stabilityii,iii
.
In this work, the preparation of 3D nanocomposites, proposed by the simultaneous photo-
reduction of metal precursors (silver acetate) with the photo-polymerization of an acrylated
monomer mixture to form a nanocomposite using the SLA technology, is presented and
discussed.
The laser beam of the printer is used to locally photo-induce the reduction of silver cations,
generating silver nanoparticles (AgNPs), while photo-polymerizing the acrylated monomers,
by means of the homolytic photo-cleavage of the photoinitiatoriv
.
The SLA printing process is used to produce specimens, tested for thermo-mechanical
characterization. Structure of AgNPs and microstructure of Ag nanocomposites are analysed
respectively using X-ray diffraction and transmission electron microscopy. The antimicrobial
and cytotoxicity tests are also performed to verify the presence of these functional properties
due to AgNPs.
i B. Utela, D. Storti, R. Anderson, M. Ganter, J. Manuf. Process. 2008, 10, 96.
ii J. Czyz˙ ewski, P. Burzyn´ski, K. Gaweł, J. Meisner, J. Mater. Process. Technol. 2009, 209, 5281.
iii D. Yugang, Z. Yuan, T. Yiping, L. Dichen, Rap. Prototyp. J. 2011, 17, 247.
iv S. Jradi, L. Balan, X. H. Zeng, J. Plain, D. J. Lougnot, P. Royer, R. Bachelot, S. Akil, O. Soppera, L. Vidal,
Nanotechnology 2010, 21, 095605-1.
A.14
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 78 Poster Communication - Session A
Design and characterization of hydrophobic and oleophobic multilayer
films by sol-gel process
R. Taurino1,2,*
, M. Messori3, F. Bondioli
1,2
1Dipartimento di Ingegneria e Architettura, Università di Parma, Parco Area delle Scienze 181/A, 43124
Parma, Italy 2Consorzio Interuniversitario Nazionale per la Scienza e Tecnologia dei Materiali (INSTM), Via G. Giusti 9,
50121 Firenze, Italy. 3Dipartimento di Ingegneria Enzo Ferrari, Università di Modena e Reggio Emilia, Via Pietro Vivarelli 10,
42025 Modena, Italy.
The development of easy-to-clean and self-cleaning surfaces is one of the important outcomes
of nanotechnology since its early beginnings. Even though a number of methods have been
reported in literature to obtain hydrophobic and oleophobic surfaces and conditions were
recognized that should be met, it has to be noted that many of them are not feasible for large-
area applications. In this work the authors evaluated the possibility to prepare multifunctional
surface through layer-by-layer deposition of organic-inorganic hybrids sol-gel films, allowing
facile control of surface roughness and hydrophobicity.
The results demonstrated that super-hydrophobic properties, and low hysteresis values (2°)
derive from combined suitable chemical compositions and surfaces roughness.
Superhydrophobic surfaces with contact angles higher than 150° were obtained after the
application of multilayer coatings, due to the presence of random irregular and fractal
surfaces. Moreover, good photocatalytic activity can be obtained after the application of TiO2-
SiO2 coating as top layer. Both hydrophobic and oleophic properties were achieved after the
application of nanostructured organic–inorganic hybrid coating based on a perfluoropolyether
oligomer (PFPE).
The method, used in this work, is simple and less expensive, compared to other conventional
techniques, and it can be applied on a variety of substrates, such as silicon wafer, glass,
metals, and polymer surfaces, with little or no pretreatment of the surfaces.
A.15
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 79 Poster Communication - Session A
Coupling nanostructured metal oxides with gold nanoparticles: a
multidisciplinary study
M. Villani1,*
, G. Bertoni1, F. Fabbri
1, L. Lazzarini
1,
D. Calestani1, N. Coppedè
1, C. Morasso
2, S. Beretta
3, F. Terenziani
3, A. Zappettini
1
1 IMEM-CNR, Parco Area delle Scienze 37/A, 43124 Parma, Italy.
2 LABION – Laboratory of Nanomedicine and Clinical Biophotonics, Fondazione Don Carlo Gnocchi
ONLUS, P.le Morandi 6, 20121, Milan, Italy. 3 Parma University, Phys. Dept., Parco Area delle Scienze 7/A, 43124, Parma, Italy.
Surface plasmon resonance is the most outstanding behavior of metallic nanostructures
resulting in optical properties hardly achievable in other optical materials, yielding a wide
range of applications. By coupling with metal oxides semiconductors, metallic
nanoparticles can be used to tune optical absorption or emission, thanks to the strong
interaction of collective excitations of free charges (plasmons) with electromagnetic fields.
Herein we present the functionalization of meaningful metal oxides nanostructures with Au
nanoparticles by means of a photochemical process which doesn’t rely on binding agents or
capping molecules to achieve shape / size control of nanostructures, resulting in clean
interfaces.
A direct imaging at the nanoscale of the plasmon-exciton coupling in Au/ZnO
nanostructures is reported by combining scanning transmission electron energy loss (EELS)
and cathodoluminescence (CL) spectroscopy / mapping. Interestingly, the Au plasmon
resonance is localized at the gold / vacuum interface, rather than presenting an isotropic
distribution around the nanoparticle [1]. Such study of the fundamental interactions at the
nanoscale allow a deeper understanding of the nanocomposite material enabling the design
of improved optical materials. As a case-study, we present a novel 3D substrate for
biomedical SERS investigations. Apomorphine detection – a drug used for the management
of Parkinson disease – has been investigated, the SERS substrate is characterized by an
enhancing factor up to 106 and a de
tested to target single cancer cells showing enhanced Raman signals related to the portion
of the cell interacting with the 3D structure of the substrate.
Figure 1. a) Sketch of ZnO TP functionalization with Au NPs by means of photochemical reaction. b)
Aqueous dispersion of ZnO/Au. c) SEM image of a single Au-decorated ZnO TP. d) A representative
Au NPs/ZnO TP as seen in HAADF-STEM.
[1] G. Bertoni, F. Fabbri, M. Villani, L. Lazzarini, S. Turner, G. Van Tendeloo, D. Calestani, S. Gradecak, A.
Zappettini, G. Salviati, Sci. Rep. 2016, 6, 19168.
[2] S. Picciolini, N. Castagnetti, R. Vanna, D. Mehn, M. Bedoni, F. Gramatica, M. Villani, D. Calestani, M.
Pavesi, L. Lazzarini, A. Zappettini, C. Morasso, RSC Adv., 2015, 5, 93644–93651.
A.16
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 80 Poster Communication - Session B
Identification of nanoparticles “protein-coronas” in the blood of marine
invertebrates: effects on the immune response
T. Balbi
1, R. Fabbri
1, M. Montagna
1, G. Camisassi
1, A. Salis
2, G. Damonte
2, L. Canesi
1,*
1Department of Earth, Environment and Life Sciences (DISTAV), University of Genoa.
2Centre of Excellence for Biomedical Research-CEBR, University of Genoa, Italy.
The development of nanotechnology will inevitably lead to the release of consistent amounts
of nanoparticles (NPs) in aquatic ecosystems, where NP intrinsic properties, as well as those
of the receiving medium, will affect particle behavior, bioavailability, uptake and toxicity in
aquatic organisms. However, the evaluation of the biological effects of NPs requires
additional understanding of how, once within the organism, NPs interact at the molecular
level with cells in a physiological environment. In mammalian models, NPs associate with
serum soluble components, organized into a “protein corona”, which affects particle
interactions with target cells. However, no information on the interactions of NPs with
biological fluids of aquatic organisms is available yet.
In the marine bivalve Mytilus, the immune system has been shown to represent a sensitive
target for different types of NPs. Data on the first identification of protein coronas formed
around different NPs (amino modified polystyrene, n-CeO2, n-TiO2) in Mytilus blood
(hemolymph) are presented. The results show that NP-protein interactions are particle
specific, and are strongly influenced by the net surface charge of the particle retained in sea
water. The role of NP-protein coronas formed in biological fluids of marine invertebrates, and
their possible consequences on the impact of NPs on innate immunity is discussed.
Canesi, L., Corsi, I., 2016. Effects of nanomaterials on marine invertebrates. Sci. Total Environ. 565, 933-940.
Canesi et al., 2016. Invertebrate models for investigating the impact of nanomaterials on innate immunity: the
example of the marine mussel Mytilus spp. Curr. Bionanotech 2, 1.
B.01
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 81 Poster Communication - Session B
LPS adsorbed to the bio-corona of TiO2 nanoparticles powerfully activates
selected pro-inflammatory transduction pathways
M.G. Bianchi
1,*, L. Paesano
2, M. Allegri
1, M. Chiu
1, G. Taurino
1, A. L. Costa
3, M. Blosi
3, S. Ortelli
3,
N. Marmiroli2, O. Bussolati*
1, E. Bergamaschi
4
1Department of Medicine and Surgery and
2Department of Chemistry, Life Sciences and Environmental
Sustainability, University of Parma, Parma, Italy.
3Institute of Science and Technology for Ceramics (CNR-ISTEC), National Research Council of Italy,
Faenza(RA), Italy. 4Department of Public Health Science and Pediatrics, University of Turin, Turin, Italy.
The adsorption of bioactive compounds provides engineered nanoparticles (NP) with novel
biological activities and may modify the biological effects of the adsorbed molecules.
Bacterial lipopolysaccharide (LPS, endotoxin), a powerful macrophage activator, is a
common environmental contaminant present in several body compartments such as the gut.
We recently observed that the co-incubation of LPS with TiO2 nanoparticles (NP) markedly
potentiates its pro-inflammatory effects on macrophages (1,2) suggesting that LPS gains
enhanced activity when included in a NP bio-corona. A pellet fraction, denominated P25/LPS,
was isolated by centrifugation from a mixture of P25 TiO2 NP (128 g/ml) and LPS (10
ng/ml) in the presence of fetal bovine serum. Western Blot analysis of the pellet eluate
indicated that P25/LPS fraction contained proteins and LPS. In murine macrophages
P25/LPS, at a nominal LPS concentration of 40 pg/ml of LPS, induced both NF-B- and
IRF3-dependent cytokines at levels comparable to those observed with free LPS (10 ng/ml),
although with different time courses. Moreover, compared to free LPS, P25/LPS caused a
more sustained phosphorylation of p38 MAPK and a more prolonged induction of STAT1-
dependent genes. Cytochalasin B partially inhibited the induction of Tnfa by P25/LPS, but not
by free LPS, and suppressed the induction of IRF3-dependent genes by either P25/LPS or free
LPS. These data suggest that, when included in the bio-corona of TiO2 NP, LPS exhibits
enhanced and time-shifted pro-inflammatory effects. Thus, in assessing the hazard of NP in
real life, the enhanced effects of adsorbed bio-active molecules should be taken into account.
1)Bianchi et al. (2015) Titanium dioxide nanoparticles enhance macrophage activation by LPS through a TLR4-
dependent intracellular pathway. Toxicol. Res. 4: 385-398. doi: 10.1039/c4tx00193a.
2) Di Cristo et al. (2016) Proinflammatory Effects of Pyrogenic and Precipitated Amorphous Silica
Nanoparticles in Innate Immunity Cells. Toxicol Sci. 150:40-53. doi: 10.1093/toxsci/kfv258.
B.02
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 82 Poster Communication - Session B
Nanoindentation of engineered bone tissue: a still unexplored option in
regenerative medicine
M. Boi
1,*, M. Bianchi
1, G. Marchiori
1, M. Berni
1, A. Russo
1, M. Sartori
1 , M. C. Maltarello
1, F.
Salamanna1, G. Giavaresi
1, M. Fini
1, M. Marcacci
1.
1 Rizzoli Orthopedic Institute, Bologna, Italy.
Bone tissue is a highly-organized material with a complex hierarchical macro-, micro- and
nanostructure. For this reason, conventional mechanical tests are not able to catch the
mechanical properties of bone on a multi-scale level. Recently, nanoindentation technique has
emerged as a useful tool for the mechanical investigation of biological materials, as it allows
to relate structural changes to variations in mechanical properties on micro- and nano-scales.
Noteworthy, the elastic modulus (ER) of bone trabeculae is strongly correlated to the
mineralization degree of the collagen fibrils of which bone is composed. To date, the
investigation of nanomechanical properties has been almost exclusively limited to the analysis
of healthy and pathologic bone tissue, whereas there are very few studies investigating the
evolution of the mechanical properties of bone tissue during the regeneration process. At the
NanoBiotechnology Laboratory of the Istituto Ortopedico Rizzoli we are currently
investigating the nanomechanical properties of engineered (i.e. regenerated) bone and
cartilage tissue according to different regeneration approaches and models, mainly involving
the use of synthetic bone grafts made of biomimetic inorganic/organic composites. By
comparing the nanomechanical properties of engineered tissue with those of native (i.e. pre-
existing) tissue, it is possible to obtain useful insights about the degree of growth of the new
tissue and efficacy of the regeneration method adopted, not directly accessible through
conventional analysis methods.
B.03
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 83 Poster Communication - Session B
Enabling development and formulation of Drug Delivery Systems based on
micro and nanoparticles by Single Particle Extinction and Scattering
(SPES) technology
C. Cella
1,*, F. Mariani
1, M.A.C. Potenza
2 , P. Milani
2, T. Sanvito
1
1 EOS
S.r.l., viale Ortles 22/4, Milan, Italy.
2 Dipartimento di Fisica, Università degli Studi di Milano, via Celoria 16, Milan, Italy.
Micro and nanoparticles in fluid suspensions are under investigation in a wide range of
applications that includes pharmaceuticals, cosmetics and agrochemicals. However, the
successful development of products based on particles is often prevented by their
unpredictable behaviors in complex fluids, such as blood and cell culture media. In fact,
particle-to-particle and particle-to-biomolecule interactions can modify particle
characteristics, surface properties and the overall engineering processes. Enabling
technologies are urgently needed for a thorough characterization of particles as suspended in
the medium where they are supposed to fulfil their function.
Traditional particle sizing techniques, such as Dynamic Light Scattering (DLS), are not able
to discriminate between particles and medium components. Hence, time consuming additional
complementary analyses are required as well as an adequate, and in many cases troublesome,
sample preparation.
We demonstrate that the innovative Single Particle Extinction and Scattering (SPES)
technology thoroughly characterizes and classifies micro and nanoparticle, even directly
suspended in complex media. Particles as model for drug delivery systems are suspended in
murine serum and blood and analyzed by SPES. Without purification, SPES technology
discriminates each single particle from the background. Moreover, fine sizing is obtained
even at very low particle concentration in media as complex as whole blood. On the contrary,
in the same experimental conditions, DLS provides untrustworthy and unrepeatable data
because of its inability to distinguish optical signals as coming from the surrounding media.
SPES opens new possibilities in micro and nanoparticle characterization and development,
including studies on pharmacokinetics, biomolecular corona formation, biomarkers and
dosimetry.
B.04
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 84 Poster Communication - Session B
Textile sensors for wearable physiological monitoring
N. Coppedè
1,*, M. Giannetto
2, A. Castellini
1,2,V. Lucchini
1,2, L. Ceriani
1,2, M. Villani
1, S. Iannotta
1, M.
Careri2, A. Zappettini
1
1IMEM CNR, Parco Area 37/a, Parma, Italy.
2Dipartimento di Scienze Chimiche, della Vita e della Sostenibilità Ambientale, Parco Area delle Scienze, 11/a,
Parma, Italy.
A growing interest in research has been devoted in the last years to the wearable applications,
in particular in the monitoring of physiological parameters. The purpose of these technologies
is to measure different biological parameters continuously in non-invasive way. If there have
been a great improvement in the monitoring of physical measurements, (Heart rate,
accelerometers, breathing, etc.) more efforts have to be done to the chemical and biochemical
side. The integration of materials for electronics and sensing in the wearable device is always
complex and implies a bridge between different technological materials. In the last years,
IMEM CNR Parma, has developed an original approach based on the direct functionalization
of textile fibers by conductive polymers, for the realization of biochemical sensing device.
The device based on an organic electrochemical transistor architecture, allows to monitor,
continuously and in non-invasive way, the electrolyte concentration in different fluids. In
particular the devices have been used to monitor human sweat. To improve the information
available from the sensor devices, further functionalization could be developed. In particular
in this work the textile electrochemical sensors, have been functionalized with ion selective
membranes, to improve the selectivity for specific ionic species. A selective device for the
sensing of potassium and another for calcium, has been realized. Moreover, the use of reactive
electrodes has been tested for the detection of specific biological molecules, present in
physiological fluids. These species are of paramount importance for monitoring the hydration
and the physiology of the athletes during sport performances of for patients or workers in
everyday life.
Battista, E., Lettera, V., Villani, M., Calestani, D., Gentile, F., Netti, P.A., Iannotta, S., Zappettini, A., Coppedè,
N. “Enzymatic sensing with laccase-functionalized textile organic biosensors” (2017) Organic Electronics:
physics, materials, applications, 40, pp. 51-57.
Coppedè, N., Tarabella, G., Villani, M., Calestani, D., Iannotta, S., Zappettini, A. “Human stress monitoring
through an organic cotton-fiber biosensor” (2014) Journal of Materials Chemistry B, 2 (34), pp. 5620-5626.
B.05
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 85 Poster Communication - Session B
β-N-Acetyl-D-mannosamine-containing calixarenes as multivalent
immunostimulators
F. Faroldi
1,*, M. Giuliani
1, S. Fallarini
3, A. Casnati
1, F. Compostella
2, G. Lombardi
3, F. Sansone
1
1Dipartimento di Scienze Chimiche, della Vita e della Sostenibilità Ambientale, Università di Parma, Parco Area
delle Scienze 17/A, 43124, Parma, Italy. 2Dipartimento di Biotecnologie Mediche e Medicina Traslazionale, Università di Milano, Via Saldini 50, 20133,
Milano, Italy. 3Dipartimento di Scienze del Farmaco, Università del Piemonte Orientale A. Avogadro, Largo Donegani 2,
28100, Novara, Italy.
Streptococcus Pneumoniae is an encapsulated bacterium responsible of several and harsh
diseases; serotype 19F is one of the most commonly isolated serotypes and it is characterised
by the presence on its capsule of a polysaccharide whose N-acetyl-D-mannosamine residue
seems to play the major role in immunostimulation and thus can be used as minimal antigenic
unit1.
We synthesized a small library of glycocalixarenes decorated with β-N-acetyl-D-
mannosamine in order to obtain immunological active compounds, exploiting multivalency.
We started from glucose to obtain β-N-acetyl-D-mannosamine, functionalized at the anomeric
position with a three-carbon spacer. The sugar unit was then conjugated with the proper
isothiocyanate calix[4] and calix[6]arenes to give the final glycocalixarenes (Figure 1). The
different geometries and valencies were chosen to study how the diverse orientation and
number of the saccharide epitopes could influence the immunological activity of these
compounds. As reported in previous works, in fact, varying the display and number of sugar
units on a calixarene platform determines interesting selectivities in the interactions with
protein receptors2,3
.
Biological properties of the glycocalixarenes have been investigated in terms of capability to
inhibit the interaction between the polysaccharide 19F and the corresponding antibody present
in the human system. Conformationally mobile calix[6]arenes have shown the best binding
affinity towards the anti-19F antibody, rising up to 80% of the inhibition shown by the natural
polysaccharide which was used as reference.
1. L. Panza, F. Ronchetti, G. Russo and L. Toma, J. Chem. Soc. Perkins Trans 1, 1987, 2745-2747; 2. F.
Sansone, E. Chierici, A. Casnati and R. Ungaro, Org. Biomol. Chem., 2003, 1, 1802-1809; 3. S. Andrè, F.
Sansone, H. Kaltner, A. Casnati, J. Kopitz, H. J. Gabius and R. Ungaro, Chembiochem, 2008, 9, 1649-1661.
n = 1, R = Me
n = 1, R = Pr, fixed cone n = 1, R = Pr, 1,3-alternate
n = 3, R = Me
Nn
Figure 1: Calixarenes decorated with β-N-acetyl-D-mannosamine
B.06
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 86 Poster Communication - Session B
3-(Mercaptopropyl)- trimethoxysilane functionalization of scaffolds based
on SiOxCy nanowires for tissue engineering applications
B. Ghezzi1,2
*, P. Lagonegro3, L. Parisi
1,2, R. Pece
1,2, C. Galli
1,2,3, G. Attolini
3, F. Rossi
3, G. M.
Macaluso1,2,3
, G. Salviati3
1Dipartimento di Medicina e Chirurgia, Università degli Studi di Parma, via Gramsci 14, 43126 Parma – Italy. 2Centro Universitario di Odontoiatria, Università degli Studi di Parma, via Gramsci 14, 43126 Parma – Italy.
3Istituto IMEM-CNR, Parco Area delle Scienze 37/A, 43124 Parma – Italy.
The aim of this study was to functionalize SiOxCy nanowires scaffolds with 3-
(Mercaptopropyl)-trimethoxysilane (Msi). Msi is a molecule with an –SH terminal group that
could provide a binding site for many biological molecules, to improve cell adhesion and
proliferation on biomaterials.
The first phase of the project was focused on the study of the topographical and chemical
surface modifications induced by the functionalization. We evaluated the wettability of the
material and its protein adsorption respectively through contact angle analysis and SDS-page
electrophoresis. The results show different wettability and protein adsorption pattern,
probably due to the –SH terminal group.
Later, the cytotoxic effect of Msi-NWs on MC3T3-E1 murine osteoblast cells was evaluated
through indirect and direct cytotoxicity contact tests, following ISO 10993-5 guidelines. To
test cell viability, we performed a chemiluminescent assay after 24 and 48h. Moreover, to
evaluate cell adhesion and proliferation the samples were investigated by fluorescence
microscopy and SEM/FIB observation. Finally, we focused our attention on genic expression
through RT-PCR, to verify if there was a stimulus to osteoblastic differentiation.
According to the observed viability, cells were more numerous on the Msi-NWS samples than
on the NWs after 48h, likely indicating that cell proliferation was more difficult on the NWs.
Fluorescence images clearly show that cells were healthier on Msi-NWS by 96h, as confirmed
also by SEM/FIB observation, suggesting that substrates have good biocompatibility and the
functionalization may offer a suitable substrate for tissue engineering applications.
B.07
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 87 Poster Communication - Session B
Bone-like apatite thin films for bone regeneration: pulsed electron
deposition from a biogenic source
G. Graziani
1,*, G. Carnevale
2, A. Pisciotta
2, L. Bertoni
2, M. Boi
1, A. Gambardella
1, M. Berni
1, G.
Marchiori1, A. Russo
1, A. De Pol
2, M. Bianchi
1
1 Rizzoli Orthopaedic Institute, NanoBiotechnology Laboratory, via di Barbiano 1/10, Bologna, Italy.
2University of Modena and Reggio Emilia, Department of Surgery, Medicine, Dentistry and Morphological
Sciences, Via del pozzo 71, Modena, Italy.
Fabrication of biogenic thin-films with suitable mechanical properties is desired in
orthopedics and dentistry to overcome the limitations of currently available coatings and
improve clinical results of coated implants.
Here, biological-like apatite thin-films were deposited at room temperature from a biogenic
source of natural apatite by pulsed electron deposition technique (PED).
Bone apatite-like (BAL) films, deposited directly from bone targets (deproteinized bovine
tibial cortical shafts), were compared to films deposited by sintered stoichiometric
hydroxyapatite targets (HA). After deposition, a set of samples was annealed at 400°C to
increase crystallinity.
All samples were characterized in terms of composition and crystallinity (XRD, FT-IR),
microstructure and morphology (SEM-EDS, AFM) and mechanical properties
(nanoindentation and micro-scratch). In vitro biological tests were then performed by using
human dental pulp stem cells (hDPSCs): the cells were isolated, plated on the samples and
cultured for 3 weeks, when the expression of typical osteogenic markers Runx-2, osteopontin,
Osx and Osteocalcin in hDPSCs was evaluated.
Results showed that deposition by PED allows obtaining bone-like apatite coatings, closely
resembling composition and structure of natural-apatite. Upon annealing at 400°C, the
coatings exhibited increased crystallinity and satisfactory mechanical properties (comparable
to those of commercial plasma-sprayed HA-coatings) and were capable of providing a
suitable microenvironment for hDPSCs adherence and proliferation and for them to reach
osteogenic commitment.
These results indicate that bone apatite-like thin films obtained by biogenic source are an
innovative and promising route for bone regeneration.
B.08
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 88 Poster Communication - Session B
Polydopamine-functionalized superparamagnetic nanocrystal clusters as
potential magnetic carriers for biomedical applications
G. Mandriota
1, R. Di Corato
1, R. Rinaldi
1*
1Dipartimento di Matematica e Fisica “Ennio De Giorgi”, Università del Salento, Via Arnesano, Lecce, Italy.
One of the major challenge of antitumor drug delivery is the development of suitable carriers
for therapeutic molecules. Superparamagnetic iron oxide nanoparticles (SPIONs) are
promising magnetic drug carriers as they are biocompatible, biodegradable, readily tunable
and controllable by an external magnetic field. We proposed and demonstrated a synthesis of
polydopamine-functionalized superparamagnetic nanocrystal clusters.
Firstly, an oil-phase evaporation-induced self-assembly strategy was introduced to fabricate
magnetic nanocrystal clusters (MNC). We demonstrated that the choice of the best size and
volume of SPIONs, solvents and the surfactant concentration are important parameters in
producing nanoclusters with a high density of magnetic cores, with a size comprised between
90 and 100 nm and a multilayer structure.
Secondly, the surface of the MNCs was functionalized with polydopamine (PDO) for
improving their stability. Different concentrations of dopamine were assayed for determining
the best compromise between stability, loading capacity and increase of the cluster size.
Thirdly, a fluorescent dye, crystal violet, was grafted to the surface of MNC@PDO as model
for drug loading and release efficiency. It is observed that the contribution of the dye
increased with higher concentration of crystal violet introduced. We demonstrated that the as
synthesized MNC@PDO can be exploited to create an environmentally sensitive magnetic
drug carrier, particularly with regards to the binding and desorption efficiency.
For the future our intention is to obtain a magnetic nanosystem that can transport
chemotherapeutic drug preferentially to its biological target by making use of magnetic field.
B.09
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 89 Poster Communication - Session B
Specific IgG antibodies react to mimotopes of BK polyomavirus, a small
DNA tumor virus, in healthy adult sera
E. Mazzoni
1, F. Frontini
1, I. Bononi
1, S. Pietrobon
1, M. Manfrini
1, G. Guerra
2, F. Martini
1,
M. Tognon1
1Laboratories of Cell Biology and Molecular Genetics, Section of Pathology, Oncology and Experimental
Biology, School of Medicine, Department of Morphology, Surgery and Experimental Medicine, University of
Ferrara, Ferrara 44121, Italy.
BK polyomavirus (BKPyV) was isolated in 1971 from the urine of a kidney transplant
patient. Soon after its identification, BKPyV was characterized as a kidney-tropic virus,
which is responsible of a significant fraction of the rejection of transplant kidney in the host.
Moreover, in experimental conditions, BKPyV is able to transform different types of animal
and human cells and to induce tumors of different histotypes in experimental animals. BKPyV
DNA sequences have been detected in healthy individuals and cancer patients using
polymerase chain reaction/Shouthern blot hybridization methods. Serum antibodies against
this polyomavirus were revealed using immunological techniques, which, however, cross-
react with other polyomaviruses such as JC (JCPyV) and Simian Virus 40 (SV40). These non-
specific data indicate the need of novel immunological methods and new investigations to
check in a specific manner, BKPyV spread in humans. To this aim, synthetic peptides of
approximately 20 a.a. residues from BKPyV structural capsid protein 1 (VP1) were employed
as mimotopes for specific immunological reactions to IgG antibodies of human serum
samples. An indirect enzyme-linked immunosorbent assay with synthetic peptides mimicking
immunogenic epitopes of BKPyV VP1 was set up and employed to test sera of healthy adult
subjects. Data from this innovative immunological assay indicate that serum antibodies
against BKPyV VP1 mimotopes are detectable in healthy subjects ranging from 18 to 90
years old. The overall prevalence of serum samples that reacted to BKPyV VP1 mimotopes
was 72%. The strong points from this investigation are (i) the novelty of the
nanobiotechnology approach, (ii) its simplicity as an immunological method, and (iii) the
specificity of BKPyV antibody reaction to VP1 mimotopes.
B.10
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 90 Poster Communication - Session B
Use of polymer conjugates for the intraperoxisomal delivery of engineered
human alanine:glyoxylate aminotransferase as a protein therapy for
primary hyperoxaluria type I
E. Oppici
1, A. Roncador
1, M. Talelli
2, A. N. Pariente
2, M. Donini
3, S. Dusi
3, C. Borri Voltattorni
1, M.
J. Vicent,2, B. Cellini
1,*
1Department of Neurological Biomedical and Movement Sciences, Section of Biological Chemistry, University of
Verona, Strada le Grazie 8, 37134 Verona (VR), Italy. 2Polymer Therapeutics Lab, Centro de Investigación Príncipe Felipe (CIFP), Av. Eduardo Primo Yúfera 3,
Valencia 46012, Spain. 3Department of Medicine, Section of General Pathology, University of Verona, Strada le Grazie 8, 37134
Verona (VR), Italy.
*present address: Department of Experimental Medicine University of Perugia, P.le Gambuli 1, Perugia.
INTRODUCTION: Alanine:glyoxylate aminotransferase (AGT) is a liver peroxisomal
enzyme whose deficit causes the rare disorder Primary Hyperoxaluria Type I (PH1). The
treatment options currently available for PH1 are either poor effective or highly invasive.
Since PH1 displays a loss-of-function pathogenesis, the development of an enzyme
administration strategy to replenish the liver with active AGT might relieve disease
symptoms. To this aim, we evaluated the possibility of delivering AGT upon conjugation with
a polymeric nanocarrier, able to promote cargo transfer across the plasma membrane, mask
the protein from the immune system and improve its stability in the bloodstream.
METHODS: We setup the conjugation of poly(ethylene glycol)-poly(L-glutamic acid) (PEG-
PGA) with AGT via formation of disulfide bonds between the polymer and solvent-exposed
cysteine residues of the enzyme.
RESULTS: PEG-PGA conjugation does not affect AGT structural/functional properties, but
allows the enzyme to be internalized in a cellular model of PH1 and restores glyoxylate-
detoxification. The insertion of the C387S/K390S amino acid substitutions, known to favour
the interaction with the peroxisomal import machinery, reduces conjugation efficiency, but
endows the conjugates with the ability to reach the peroxisomal compartment. The obtained
conjugates are hemocompatible, stable in human plasma and not immunogenic.
DISCUSSION: These results hold promise for the development of polymer-conjugated AGT
as an enzyme administration therapy for PH1 patients and represent the base for the
application to other diseases due to the deficit of peroxisomal proteins.
B.11
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 91 Poster Communication - Session B
CeF3 – ZnO nanostructures for the self-lighted photodynamic therapy of
deep tumors
D. Orsi
1,*, T. Rimoldi
1, S. Pinelli
2, R. Alinovi
2, G. Benecchi
3, F. Rossi
4, L. Cristofolini
1
1 Department of Mathematical, Physical and Computer Sciences, University of Parma, Parma, Italy.
2 Department of Medicine and Surgery, University of Parma, Parma, Italy.
3 Servizio di Fisica Sanitaria, Azienda Ospedaliero-Universitaria di Parma, Parma, Italy.
4 IMEM-CNR Institute, Parma, Italy.
Self-Lighted Photodynamic Therapy (SLPDT) is a proposed approach to the treatment of
deep tumors which aims at combining low-dose radiation therapy and photodynamic
therapy (PDT). A penetrating high-energy radiation (typically, X-rays) excites a
nanostructure that, combining a scintillating nanomaterial and a photosensitizer, can
damage tumor cells via the generation of reactive oxygen species (ROS) such as singlet
oxygen (1O2). Hence, SLPDT can be applied to deep tumors, where direct illumination of
the photosensitizer as in usual PDT is unfeasible.
We developed an inorganic nanostructure for SLPDT made of CeF3 nanoparticles
embedded in a nano-sized ZnO matrix. CeF3 is an efficient scintillator: when illuminated by
X-rays it emits UV light at 325 nm. ZnO is photo-activated in cascade, to produce ROS. In
contrast with hybrid organic-inorganic nanostructures, the inorganic nature of the NS
overcomes the problem of radiation-induced degradation,
which is known to affect organic photosensitizers.
The nanostructures have been tested in-vitro on
adenocarcinoma cells (A549). They show high internalization
and cytocompatibility in dark at concentrations > 50µg/ml.
Irradiation with X-rays induces ROS and singlet oxygen
generation; this reduces A549 cells’ viability and blocks the
cellular cycle in phase G2-M. This suggests that the
nanostructures could be effectively used to hinder tumor
proliferation between irradiation sessions, thus enhancing the
effectiveness of radiotherapy.
B.12
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 92 Poster Communication - Session B
Markers for toxicity to HepG2 exposed to cadmium sulphide quantum dots;
damage to mitochondria
L. Paesano
1,*, A. Perotti
1, A. Buschini
1, C. Carubbi
2, M. Marmiroli
1, E. Maestri
1, S. Iannotta
3, N.
Marmiroli1
1Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parma, Italy.
2Department of Medicine and Surgery, University of Parma, Parma, Italy.
3IMEM-CNR, Parma, Italy.
Interaction of living organisms with quantum dots (QDs) is certainly more focused on
environment and occupational exposure associated with production and release or disposal.
Here, the transcription of genes involved in mitochondrial organization and function in
HepG2 cells exposed to cadmium sulphide (CdS) QDs has been profiled to highlight
biomarkers of exposure and effect to be tested for other cadmium based QDs. At low
concentrations, exposure to CdS QDs induced only minor damage to nuclear DNA, and none
to mitochondrial DNA. However, the stress caused an increase in the production of reactive
oxygen species (ROS), which triggered the mitochondria-mediated intrinsic apoptotic
pathway involving a cascade of transcriptomic events, finally prompting the activation of a
rescue pathway. The transcriptomic analysis confirmed the involvement in the response to
CdS QDs of genes related to apoptosis (AIFM2 and APAF1), oxidative stress response
(OXR1 and AOX1) and autophagy (ATG3 and ATG7), as potential biomarkers. Other
possible biomarkers specific for mitochondria function were LONP1 and HSPD1.
B.13
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 93 Poster Communication - Session B
Nebulized coenzyme Q10 nanosuspensions: a versatile approach for
pulmonary antioxidant therapy
I. Rossi
1, E. Quarta
1, F. Sonvico
1, F. Buttini
1*
1Food and Drug Department, University of Parma, Parco Area delle Scienze 27/A, Parma, Italy.
Coenzyme Q10 (CoQ10) is an antioxidant substance indicated as a dietary supplement that has
been proposed as adjuvant in the treatment of cardiovascular disorders and cancer for its
protective and immunostimulating activities. The aim of this work was the production by
high-pressure homogenization, characterization and stability study of three different CoQ10
nanosuspensions designed to be nebulised.
Three surfactants, i.e. lecithin, PEG32 stearate and Vitamin-E TPGS, were selected to
stabilize CoQ10 formulations. Preparations were identified as nanosuspensions (particle size in
the range 35 - 60 nm): the smallest particles were obtained with Vitamin-E TPGS and denoted
a core-shell structure. The CoQ10 delivered from a commercial air-jet nebulizer was in all the
cases around 30% of the loaded dose. The nanosuspension containing PEG32 stearate
presented the highest respirable fraction (70.6% ± 5.1) and smallest median mass
aerodynamic diameter (3.02 µm ± 0.49). Stability tests showed that the most stable
formulation, after 90 days, was the one containing Vitamin-E TPGS, followed by the CoQ10-
lecithin formulation. Interestingly, those formulations were demonstrated to be suitable also
for nebulization with other type of devices, such as ultrasound and vibrating mesh nebulizers.
Studies focused on in vitro cellular toxicity of the formulations and their single components
using A549 human lung cells showed no obvious cytotoxicity for the formulations containing
lecithin and PEG32 Stearate. Vitamin-E TPGS alone was shown to be able to damage the
plasma membrane, nevertheless, cell damage was decreased when Vitamin-E TPGS was
present in the formulation with CoQ10.
B.14
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 94 Poster Communication - Session B
Liquid-gated field-effect transistors based on reduced graphene oxides as
sensing platform for biomolecules
A. Rozzi
1,2, J. Brintinger
1,3, K. Shabnam
1, P. Rudatis
1,3, N. Yevalik
1,3, Z. Valeriia
1, W. Knoll
1, R.
Corradini2*
1Austrian Institute of Technology, Donau-City-Straße 1, 1220 Vienna, Austria.
2Università di Parma, Parco Area delle Scienze 11/a, 43124 Parma, Italy.
3Technische Universität Wien, Karlsplatz 13, 1040 Vienna, Austria.
Since its discovery in 2004 by Gejm and Novoselov, the interest on graphene is growing not
only on its pure electronic properties but also to use it for sensing and bio-sensing
applications. The so-called FETs (field-effect-transistors) are a special type of sensor that
are highly sensitive to the surface modification in terms of change of charge. Using
reduced-oxide graphene (rGO) flakes it is possible to create a monolayer of graphene that
can be furthermore functionalized by bio-recognition units with specific interactions with
analytes. These specific interactions changes the electronic properties of the underlying
graphene, thus resulting in a modulation of the read out current.1 We report on the
fabrication, optimization and functionalization of rGO based FETs in a liquid gated
configuration (Figure 1) used for label-free detection of DNA, that is a powerful tool for
genetic disease, pathogen and genetic-modified detection. The bio-recognition units for the
detection are PNAs (Peptide Nucleic Acids) some synthetic analogous of DNA that show
high affinity and selectivity for the target sequence2, due to a neutral backbone that
minimize electrostatic repulsion between the hybridized strands (Figure 2).
References:
1. Larisika M, Kotlowski C, Steininger C, Mastrogiacomo R, Pelosi P, Schütz S, et al. Angewandte
Electronic Olfactory Sensor Based on A . mellifera Odorant-Binding Protein 14 on a Reduced
Graphene Oxide Field-Effect Transistor Angewandte. 2015;13443–6.
2. Cai B, Wang S, Huang L, Ning Y, Zhang Z, Zhang G. Ultrasensitive Label-Free Detection of PNA À
DNA Hybridization by Reduced Graphene Oxide Field-E ff ect Transistor. 2014;(3):2632–8.
Figure 2: Hybridization between PNA and
DNA
Figure 1: rGO based FETs in a liquid gated
configuration
B.15
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 95 Poster Communication - Session B
Ultrastructural study of biomineralization process in human bone marrow
mesenchymal stem cells during the osteoblastic differentiation
A. Sargenti
1,*, S. Iotti
2, C. Cappadone
1, G. Farruggia
1, A. Procopio
1, E. Malucelli
2
1Dipartimento di Farmacia e Biotecnologie, Università di Bologna,, Via San Donato 19/2, Bologna, Italy. 2Dipartimento di Farmacia e Biotecnologie, Università di Bologna, Via San Donato 15, Bologna, Italy.
Calcium is fundamental for the bone and it is present in the extracellular mineralized matrix
as hydroxyapatite (HA) crystals. Very little is known about the intracellular Ca concentration,
distribution and homeostasis in bMSC, and on the progression of the extracellular Ca-
phosphates and polyphosphates deposition during osteoblast differentiation. Synchrotron X-
ray radiation sources allow to study at nanoscale the cellular content and the extracellular
deposition of the elements involved in the HA formation.
The goal of this study is to measure the intracellular Ca concentration and the extracellular
Ca, P and Zn deposition in human bMSC induced to osteoblast differentiation at different
points. Cryofixed samples have been studied at the beamline ID16A-NI at the ESRF
synchrotron. We combined X-ray Fluorescence Microscopy measurements with X-ray phase
contrast nano-tomography, to obtain 2D Ca, P and Zn concentration maps at nanoscale
resolution (fig.1). To overcome misleading interpretation coming from 2D elemental maps,
we acquired x-ray fluorescence tomography to better localize in the space the deposition of
Ca, P and Zn.
The preliminary results of the 2D fluorescence shown an early spot deposition of Ca already
at 4 days. It is worthy to note that in correspondence of Ca deposition a P and Zn
accumulation is present as well. Moreover, the extracellular deposition of Ca at 10 days is
massive since the bMSC osteoblast differentiation is almost complete and the co-localization
of Ca with P and Zn is still evident.
These results strongly suggest the presence of phosphate compounds of Ca precursors of the
HA formation.
Figure 1.fluorescence maps of K, Ca, P and Zn (respectively panel a, b, c, and d) in bMSC after 4 (on the left)
and 10 (on the right) days of differentiation.
B.16
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 96 Poster Communication - Session C
Estimating consumer exposure to silver nanoparticles from food contact
materials: the study of migration properties
S. Argentiere1,*
, C. Cella2, C. Lenardi
3
1Filarete Foundation, viale Ortles 22/4, 20139, Milan, Italy.
2EOS Srl, Viale Ortles 22/4, 20139 Milan, Italy.
3 Cimaina and Physics Department, University of Milan, via Celoria 16, 20133, Milan, Italy.
Due to their antimicrobial, anti-fungi, anti-yeasts and anti-viral activities, the use of silver
nanoparticles (AgNPs) in food contact materials (FCM) is emerging as an effective strategy to
prolong the shelf life of fresh food products. However, concerns are raised about the AgNPs
potential for adverse effects in humans, therefore risk assessment strategies are required to
estimate consumer exposure to AgNPs from FCM.
In this study, 100 nm AgNPs coated with either citrate (CT-AgNPs) or polyvinylpyrrolidone
(PVP-AgNPs) were selected. The migration properties of AgNPs were studied as a function
of different experimental parameters (particle surface coating, pH and ionic strength of
suspending medium), using a method based on shake flask principle. Finally, the impact of
the protein corona in AgNPs migration was evaluated.
After three hours shaking, the partition equilibrium was reached for both CT- and PVP-
AgNPs. However, the PVP-AgNPs completely moved in the organic phase, while the CT-
AgNPs still remained in the aqueous one. Nevertheless, this distribution was dependent on pH
and ionic strength of the aqueous phase: the higher the pH the more the AgNPs migrated in
the aqueous phase, both for CT and PVP coating. Finally, the formation on purpose of a
protein corona around each single AgNP minimized differences in AgNPs phase distribution
since both CT- and PVP-AgNPs were stabilized in the aqueous phase at each pH tested.
C.01
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 97 Poster Communication - Session C
Characterization of nutritional and health properties of local Tuscany
cherry varieties and evaluation of innovative nanosystems delivery
D. Beconcini
1,2,*, R. Berni
2, F. Felice
1, M. Romi
2, A. Fabiano
3, Y. Zambito
3, R. Di Stefano
1, T.
Santoni1, G. Cai
2, C. Cantini
4
1University of Pisa, Department of Surgery, Medical, Molecular, and Critical Area Pathology, via Paradisa 2,
Pisa, Italy. 2University of Siena, Department of Life Sciences, via Aldo Moro (San Miniato) 2, Siena, Italy.
3University of Pisa, Department of Pharmacy, via Bonanno 33, Pisa, Italy.
4CNR Ivalsa Follonica, via Aurelia 49, Follonica (GR), Italy.
Purpose: Oxidative stress plays an important role in the pathogenesis of various
cardiovascular diseases, including atherosclerosis. HUVECs (Humbilical Vein Endothelial
Cells) are very sensitive to injury caused by an high concentrations of reactive oxygen species
(ROS). Numerous products of Tuscan agro-food are rich in antioxidant and biologically
active substances. Among these, cherries are rich in polyphenols and especially in
anthocyanins. Aim of this work is to evaluate the antioxidant power and the protective effect
of natural cherry extracts (CE) on HUVECs.
Methods: Crognola Capannile, an autochtonous tuscany variety of Prunus Avium L. (sweet
cherry), was processed and its extract explored to evaluate the total polyphenolic content and
the antioxidant capacity, using Folin-Ciocalteau and FRAP assay methods. CE cytotoxicity on
HUVECs was evaluated by WST-1 assay. ROS production in vitro will be evaluated with the
use of a fluorescent probe (CM-H2DCFDA). CE will be encapsulated in multifunctional
chitosan-derivatives nanoparticles.
Results: Total polyphenolic content and antioxidant capacity were respectively 402,5 mg
Gallic Acid Equivalents (GAE) per 100 g of fresh weight (FW) and 2,19 mmol of ferric
chloride per 100 g of FW (as shown in TABLE 1). CE was mainly composed by phenolic
acids and antocyanins such as ferulic acid and cyanidin-3-glucoside (CYN3G), identified by
HPLC. CE added to endothelial cells culture were not cytototoxic until the polyphenolic
concentration of 500 μg GAE/ml of culture medium (GRAPHIC 1). ROS production results
are currently under evaluation.
Conclusions: Starring in the food market only seasonally, cherries have become nutraceutical
research topics for their possible medical applications in cardiovascular diseases with an
appropriated nanosystem delivery. GRAPHIC 1: Cherry extract cytotoxicity. HUVECs were cultured for 2 h in the
presence of CE (from 1µg/ml up to 1 mg/ml of total polyphenols). Cell viability was
determined by WST-1 colorimetric assay and expressed as % viability compared to
control (untreated cells). Data are representative of three separate experiments in
triplicate
TABLE 1: Total antioxidants, total phenolics and total antocyanins estimated in Crognola Capannile. Antioxidants
(mmol Fe3+/100 g FW)
Phenolics
(mg GAE/100g FW)
Antocyanins
(mg CYN3G/100 g FW)
Crognola Capannile
(Prunus Avium)
2,19 ± 0,09
402,5 ± 8,4
166,5 ± 2,4
0%
20%
40%
60%
80%
100%
120%
Ctr
1µg/
ml
5µg/
ml
30µg
/ml
50µg
/ml
100µ
g/ml
500µ
g/ml
1000
µg/m
l
Polyphenols concentrations
Cell
via
bil
ity (
%)
C.02
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 98 Poster Communication - Session C
Preparation of cellulose nanocrystals for decayed old wood consolidation
L. Bergamonti1,2
, F. Bondioli2, C. Graiff
1, A. Haghighi
3, C. Isca
1,P. P. Lottici
4, B. Pizzo
5, G. Predieri
1*
1Dip. SCVSA, Università di Parma, Parco Area delle Scienze 11/A, Parma, Italy.
2Dip. di Ing. e Arch., Università di Parma, Parco Area delle Scienze 181/a, Parma, Italy.
3Dept. of Wood Science and Technology, University of Tehran, Karaj, Iran.
4Dip. SMFI, Università di Parma, Parco Area delle Scienze 7/A, Parma, Italy.
5 CNR IVALSA, via Madonna del Piano 10, Sesto Fiorentino, Italy.
Wood is one of the oldest materials used in a large variety of human artifacts thanks to its
particular aesthetic characteristics and mechanical properties. Wood is an organic,
hygroscopic and anisotropic material and for its nature is subjected to physical, chemical and
biological degradation. Current treatments adopted for historical wood conservation often
have undesirable side-effects which can have an impact on the aesthetical and mechanical
characteristics of the wooden artifacts.
This work deals with an attempt to obtain new nature-inspired consolidants compatible with
wood and paper, by using cellulose nanocrystals (CNC), a new class of cellulose materials
that have found a lot of applications in various research areas over the past two decades. CNC
can be obtained by acid hydrolysis of cellulose extracted by different materials, i.e. wood,
cotton, hemp [1].
CNC are expected to enhance the fiber-fiber bond strength and, hence, have a strong
reinforcement effect on paper materials. The main goal of this study is to test the consolidant
efficacy of the CNC on rotted wood samples to improve their mechanical properties.
Suspensions of cellulose nanocrystals (CNC) were prepared by sulfuric acid hydrolysis
starting from α-cellulose. The crystalline nature of nanocellulose was confirmed by XRD
analysis.
The CNC sol was applied on wood sample by total impregnation, under vacuum. The
products tested were CNC and CNC mixed
with lignin and/or PDMS. The mechanical
tests were carried out on a dynamic
mechanical analyzer (DMA) in three-point
bending. The best results in consolidation
efficiency (% improvement of elastic
modulus, see Figure) have been obtained with
CNC alone. Actually, CNC appreciably
improves the stiffness properties of the
decayed wood, in particular in the case of the
maximum decay class (A). Minor results have
been obtained in the case lower decay classes
(B, C).
[1] Y. Habibi Y., Key advances in the chemical modification of nanocelluloses. Chem. Soc. Rev. 2014, 43,
1519-1542
0
50
100
150
200
250
0 5 10 15 20 25 30 35
Relativeincrease,%
Numberofrepeatedimpregnationcycles
StoragemodulusrelativeincreaseA
B
C
C.03
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 99 Poster Communication - Session C
From field to fork: determination of metal nanoparticles in the pasta food
chain
F. Bianchi1,*
, M. Mattarozzi1,2
, D. Catellani3, M. Suman
3, M. Careri
1,2
1Dipartimento di Scienze Chimiche, della Vita e della Sostenibilità Ambientale, Università di Parma, Parco Area
delle Scienze 17/A ,43124 Parma, Italy. 2Centro Interdipartimentale SITEIA.PR, Università di Parma, Parco Area delle Scienze 181/A, 43124 Parma,
Italy. 3Barilla G.R. F.lli SpA, Advanced Laboratory Research, Via Mantova, 166 – 43122 Parma, Italy.
Nanoparticle research is currently an area of intense scientific research due to a wide variety
of potential applications in biomedical, optical, electronic and food areas. Nanoparticles (NPs)
can be produced from human activities both intentionally and unintentionally, so they can
enter the food chain, thus contaminating food products. Both Electron Microscopy equipped
with Energy Dispersive X-Ray spectroscopy (EDS) and Inductively Coupled Plasma-Mass
Spectrometry can be used to visualize NPs, to determine shape, size and aggregate state and
to assess their elemental composition, thus allowing to perform both qualitative and
quantitative analyses. In this study, Environmental Scanning Electron Microscopy (ESEM)-
EDS and ICP-MS were used to assess the presence of NPs both in air and in the pasta food
chain. Both qualitative and quantitative analyses of NPs in raw materials (wheat ear, wheat,
semolina) and spaghetti were performed. Particle counting and identification were performed
on automatically acquired images over proper representative filter area. As for raw materials
and pasta, polycarbonate 0.1 µm filters were used to collect the particles after immersion of
the food products in milli-Q water. Fe and Ti were the major constituents of the collected
particles: Fe particles were mostly present in wheat ear and wheat, whereas Ti particles in
pasta samples due to the use of a wire-drawing machine containing Ti.
Finally, air monitoring near pilot plants was also performed by using a eight-stage Andersen
cascade impactor and then submitting the filters to ESEM-EDS, ICP-MS and gravimetric
analyses. Particles containing mainly Fe, Mn and Pb were identified.
C.04
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 100 Poster Communication - Session C
Characterization of mutants tolerant to cadmium sulphide quantum dots
M. Caldara1,*
, C. Agrimonti1, A. Zappetini
2, N. Marmiroli
1
1Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma
2IMEM-CNR, Parma, Italy.
Metal-based nanomaterials are hydrophilic, and are typically only poorly soluble. Here, we
are focusing on cadmium sulphide quantum dots (CdS QDs). Its use is increasing,
particularly in the electronics industry. Their size (1-10 nm in diameter) is, however, small
enough that they can be taken up by living cells. The yeast Saccharomyces cerevisiae
represents an incredible toolbox for the dissection of complex biological processes, and how
they could be modified by the presence of different molecules or materials3.This model
organism displays sensitivity to CdS QDs. Recently4, this sensitivity has been attributed to a
cascade triggered by oxidative stress leading to apoptosis. Indeed, the effect of CdS QDs
exposure in yeast increases the level of reactive oxygen species, decreases the level of
reduced vs oxidized glutathione; reduces oxygen consumption, and the abundance of
respiratory cytochromes; disrupts mitochondrial membrane potentials, and affects
mitochondrial morphology. Previously5, the S. cerevisiae deletion mutant collection has been
exploited to provide a high-throughput means of revealing the genetic basis for
tolerance/susceptibility to CdS QDs exposure. The network analysis created with the
‘‘tolerance’’ mutations as entries, clustered genes encoding proteins involved in vacuolar
transport, morphology, inheritance in histone acetylation, in ion transport, in the DNA
damage response, and in protein folding.
To understand better the reason for their phenotype, we are now investigating the effects that
CdS QDs have on “tolerant mutants”. Preliminary data show that these strains display the
same oxidative stress as the wild type control. This seems to suggest that tolerance might be
connected to downstream effects that need to be further investigated.
3: Caldara M et al., 2017 Toxicological Sciences
4: Marmiroli M. et al., 2015 Nanotoxicology
5: Pasquali F. et al., 2017 J. Hazard. Mater.
C.05
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 101 Poster Communication - Session C
Nanoporous polyanilines for pollutants removal from air and wastewater
E. Falletta*, C. Della Pina
1Chemistry Department, Università degli Studi di Milano, via C. Golgi, 19, 20133, Milan, Italy.
The progressive growth of world population and industrialization have caused numerous
emerging problems, e.g., environmental pollution, that threaten human health and life quality.
Pollutants removal from air, soil and wastewater has become an important objective to be
accomplished by innovative nanomaterials able to remove dyes, metals, and so on [1].
Conducting polymers, as polyaniline and polypyrrole, have attracted a huge interest owing to
their peculiar properties which make them valid substitutes of conventional materials in
different fields. These compounds are generally synthesized following old polluting
processes. Inspired by our long-standing experience in catalysis [2], we have recently
addressed our efforts to find novel “green” approaches to produce them [3] and extend their
application in the environmental field.
Herein, we report our recent achievements in the VOCs monitoring and removal from air, as
well as in chromium and dyes removal from wastewater. In both cases nanoporous
polyanilines, produced either by traditional or clean protocols, have been employed as the
sorbent supports. The best materials were subjected to easy regeneration processes and, once
reused, they were found to retain their original high performances.
[1] G. Z. Kyzas, K. A. Matis, J. Mol. Liq., 2015, 203,159;
[2] C. Della Pina, E. Falletta, M. Rossi, Chem. Soc. Rev., 2012, 41, 350;
[3] C. Della Pina, E. Falletta, M. Rossi, Cat. Today, 2011, 160, 11.
C.06
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 102 Poster Communication - Session C
Proteomic analysis of CdS QDs response in Saccharomyces cerevisiae
V. Gallo1*
, M. Marmiroli1,V. Srivastava
2, N. Marmiroli
1
1. Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parco Area
delle Scienze 33/A, 43124 Parma, Italy. 2. Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University
Centre, 106 91 Stockholm, Sweden.
Cadmium sulfide quantum dots (CdS QDs) are widely used in the electronic industries to
produce semiconductor structures, solar energy and medical devices. It has been shown that
CdS as nanoscale material has different physical and chemical properties than its "bulk"
counterpart. The aim of the research is to evaluate the response of Saccharomyces cerevisiae
model system to CdS QDs, exploiting proteomic approaches. A comparative study on the two
quantitative methods frequently used in proteomics, 2- DE (dimensional gel electrophoresis)
and iTRAQ (isobaric tags for relative and absolute quantification), was carried out. The first
method is familiar techniques used in gel based quantitative proteomic, the second method is
a new LC (liquid chromatography) -based technique which is gradually gaining in popularity.
The proteomic analysis was performed in yeast cells collected in the exponential and in the
stationary phases of growth in liquid YPD (yeast extract peptone dextrose), without
supplementation, with 0.25 mg L−1 nystatin, with 100 mg L−1 CdS QDs and with 0.25 mg
L−1 nystatin plus 100 mg L−1 CdS QDS. This comparative analysis was used to identify
differences in protein abundance with respect to the treatments and at the stage of growth:
exponential and stationary. The data were analyzed through different bioinformatics tools to
identify the proteins and the main pathways of response to CdS QDs. The results obtained
could provide mechanisms information on biological process and genes involved in response
in yeast and, in general, in eukaryotes.
C.07
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 103 Poster Communication - Session C
The proteomic response of Arabidopsis thaliana to cadmium sulfide
Quantum Dots and its correlation with the transcriptomic response
D. Imperiale1,2,3,*
, M. Marmiroli1, L. Pagano
1, M. Villani
4, A. Zappettini
4, N. Marmiroli
1,2,3
1Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parco Area
delle Scienze 33/A, Parma, Italy. 2 Interdepartmental Center Siteia.Parma, University of Parma, Parma, Italy. 3 CINSA,
National Interuniversity Consortium for Environmental Sciences
4 IMEM-CNR, Parma.
A fuller understanding of the interaction between plants and engineered nanomaterials is of
topical relevance because the latter are beginning to find applications in agriculture and the
food industry. There is a growing need to establish objective safety criteria for their use. The
recognition of two independent Arabidopsis thaliana mutants displaying a greater level of
tolerance than the wild type plant to exposure to cadmium sulfide quantum dots (CdS QDs)
has offered the opportunity to characterize the tolerance response at the physiological,
transcriptomic and proteomic levels. Here, a proteomics-based comparison confirmed the
conclusions drawn from an earlier transcriptomic analysis that the two mutants responded to
CdS QD exposure differently both to the wild type and to each other. Just over half of the
proteomic changes mirrored documented changes at the level of gene transcription, but a
substantial number of transcript/gene product pairs were altered in the opposite direction. An
interpretation of the discrepancies is given, along with some considerations regarding the use
and significance of -omics when monitoring the potential toxicity of ENMs for health and
environment [1].
[1] Marmiroli M, Imperiale D. et al. Front. Plant Sci. 6:1104 (2015) doi:10.3389/fpls.2015.01104
C.08
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 104 Poster Communication - Session C
Germination of common velvet grass (Holcus lanatus L.) and dandelion
(Taraxacum officinale F. H. Wigg) as affected by nCeO2 of different size
D. Lizzi
1,2, A. Mattiello
2, L. Marchiol
2*
1Department of Life Sciences, University of Trieste, via L. Giorgieri 10, I-34127, Trieste, Italy.
2DI4A-Department of Agriculture, Food, Environment and Animal Sciences, University of Udine, via delle
Scienze 206, I-33100 Udine, Italy.
Nanomaterials from industrial, and urban sources may reach aquatic and terrestrial
environments. The knowledge about the relationships between nanomaterials (NMs) and
biota still remains incomplete. However, it is known that NMs can explicate their actions
depending on both the chemical composition and on the size and/or shape of the
nanoparticles (NPs). With regard to plants size and shape of NPs can have a role in
influencing their bioavailability and uptake by plants. Here we show preliminary data
regarding the effects of cerium oxide nanoparticles (nCeO2) of different size (25 and 50
nm) on germination and root elongation of common velvet grass (Holcus lanatus L.) and
dandelion (Taraxacum officinale F. H. Wigg). These species were chosen since they are
very common plants easy to identify, highly competitive among crop and weed species and
greatly adaptable to different ecological conditions. Also, they are characterized by easy
germination and rapid growth. Germinating seeds were exposed to different concentrations
(0, 0.2, 2, 10, 20, 50, 200, 2000 mgL-1
) of nCeO2 and bulk CO2, respectively. Relative Seed
Germination (RSG), Relative Root Elongation (RRE) and Germination Index (GI) were
assessed after 10 days. Cerium concentration in seedlings was determined by means of ICP-
MS.
C.09
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 105 Poster Communication - Session C
CdS QDs and CdSO4: oxidative stress-induced toxicity in A. thaliana w.t.
F. Mussi1, D. Imperiale
1, G. Lencioni
1, A. Zappettini
2, M. Villani
2, N. Marmiroli
1, M.
Marmiroli1*
1Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parco Area
delle Scienze 33/A, 43124 Parma, Italy. 2Institute of Materials for Electronics and Magnetism (IMEM), National Research Council (CNR), Parco Area
delle Scienze 37/A, 43124 Parma, Italy.
Plants of A. thaliana (L. erecta) w.t. were tested in vitro at three growth stages (15, 30, 45
days) with different concentrations of either CdS quantum dots or CdSO4. The contaminants
were supplemented in agarised MS medium at two subtoxic concentrations corresponding to
1/3 and 2/3 MIC (Minimal Inhibitory Concentration). Plants were analyzed for the content of
Cd, chlorophylls, carotenoids and total phenolics in addition to the leaf respiration rate (TTC
assay), the antioxidant activity (ABTS and DPPH assay) and oxidative stress parameters
(GSH redox state and lipid peroxidation). ESEM/EDX was used to detect morphological
changes at the level of organs and tissues; microanalysis was performed to verify Cd uptake
in roots and leaves estimating root-shoot translocation, and to identify possible sinks for Cd
storage. These parameters were used to establish a correlation between the oxidative stress
response of plants to CdS QDs or Cd bulk material, at different concentrations and treatment
time. Considering the natural senescence process of A. thaliana (particularly evident after 45
days), we evidenced substantial differences in the response of plants to the two types of
contaminants. Cd was detected in roots and leaves, especially in trichomes, of plants treated
with high CdS QDs. Oxidative stress in plants appeared as a mechanism of CdS QDs toxicity,
increasing with higher CdS QDs concentrations. Comparing CdSO4 and CdS QDs toxic
effects significant differences emerged in relation to the type and time of treatment: CdSO4
was more toxic and caused higher levels of oxidative stress than CdS QDs.
C.10
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 106 Poster Communication - Session C
Nucleo-mitochondrial interaction of yeast in response to cadmium sulfide
quantum dot exposure
F. Pasquali1, C. Agrimonti
1, L. Pagano
1,2,*, A. Zappettini
3, M. Villani
3, M. Marmiroli
1, J. C. White
2, N.
Marmiroli1
1Dept. Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parma, Italy
2The Connecticut Agricultural Experiment Station, New Haven, CT, USA.
3IMEM-CNR, Parma, Italy.
Cell sensitivity to quantum dots (QDs) has been attributed to a cascade triggered by oxidative
stress leading to apoptosis. The role and function of mitochondria in animal cells are well
understood but little information is available on the complex genetic networks that regulate
nucleo-mitochondrial interaction. The effect of CdS QD exposure in yeast Saccharomyces
cerevisiae was assessed under conditions of limited lethality (<10%), using cell physiological
and morphological endpoints. Whole-genomic array analysis and the screening of a deletion
mutant library were also carried out. The results showed that QDs: increased the level of
reactive oxygen species (ROS) and decreased the level of reduced vs oxidized glutathione
(GSH/GSSG); reduced oxygen consumption and the abundance of respiratory cytochromes;
disrupted mitochondrial membrane potentials and affected mitochondrial morphology.
Exposure affected the capacity of cells to grow on galactose, which requires nucleo-
mitochondrial involvement. However, QDs exposure did not materially induce respiratory
deficient (RD) mutants but only RD phenocopies. All of these cellular changes were
correlated with several key nuclear genes, including TOM5 and FKS1, involved in the
maintenance of mitochondrial organization and function. The consequences of these cellular
effects are discussed in terms of dysregulation of cell function in response to these
“pathological mitochondria”.
C.11
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 107 Poster Communication - Session C
Ring-shaped proteins bind nanoparticles and affect their bioactivity
G. Pira1, M. Villani
2, A. Zappettini
2, N. Marmiroli
1,*, R. Ruotolo
1,*
1Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parco Area
delle Scienze 11/A, Parma, Italy. 2IMEM-CNR, Parma, Parco Area delle Scienze 37/A, Parma, Italy.
Nanotechnology is an emerging branch of applied science and technology for designing tools
and devices of size 1-100 nm. Engineered nanoparticles (NPs) have been widely used in
several fields from medical to electronics. The increasing interest for these advanced
technologies has led to great excitement about potential benefits, but little is known about the
potential effects of NP exposure on environment and human health. Toxicity and
biocompatibility of these materials may depend predominantly on the formation of protein
corona, which influence cell interactions, localization and bioactivity of NPs. The aim of this
work is to study the interactions of CdS quantum dots (QDs) and yeast cells (Saccharomyces
cerevisiae). We employed a proteomics-based approach coupled with MS analysis to
determine the identities of proteins that form the hard corona of these NPs. We observed that
ring-shaped proteins involved in specific cellular pathways, as protein synthesis, are more
prone to bind on NPs. Electrostatic and hydrophobic interactions are critical in the formation
of the protein corona. We also focused on the possible toxicological implications of the QD-
corona formation in yeast. Yeast mutant strains deleted in genes coding for corona proteins
show a tolerant phenotype also in the presence of QD concentrations that suppress the
viability of the wild-type strain. Tolerant phenotype of these mutants suggests that the
formation of protein corona may mediate the cytotoxicity of QDs in yeast. This study
demonstrate that the characterization of the protein corona would be a relevant approach to
predict potential toxicological effects of the NPs.
C.12
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 108 Poster Communication - Session C
CdS QDs induce mutagenesis and affect spore morphogenesis in
Saccharomyces cerevisiae
R. Rossi1, M. Villani
2, A. Zappettini
2, N. Marmiroli
1,*, R. Ruotolo
1,*
1Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parco Area
delle Scienze 11/A, Parma, Italy. 2IMEM-CNR, Parma, Parco Area delle Scienze 37/A, Parma, Italy.
Nanotechnology is a rapidly growing field having potential applications in many areas. Metal-
based nanoparticles (NPs) have been studied for cell toxicity, immunotoxicity and
genotoxicity, but their real effects and the molecular mechanisms are still not fully known. In
this work, we studied the genotoxic effect of CdS quantum dots (QDs) in the yeast
Saccharomyces cerevisiae. The reversion of haploid and diploid strains carrying the ade2-
1 allele is analyzed upon QD treatment. We show that these metal-based NPs induce an
increase in mutability of both nuclear ochre nonsense alleles. Ade+ revertants grow more
slowly than the wild-type strain and it is possible that CdS QDs induce several mutations in
yeast genome. Future studies will indicate whether the mutagenic effect leads to perturbations
of replication and/or DNA repair synthesis. We have also studied the effects of these NPs on
meiotic development (sporulation) in yeast. The meiotic cell cycle is regulated through an
intricate developmental program that culminates in the production of highly specialized cell
types, spores, in budding yeast. CdS QDs inhibit progression through meiosis in an
irreversible manner with adverse effects on meiotic cell cycle progression and spore
formation. High concentrations of QDs (>3 mg/L) cause cell arrest and complete inhibition of
spore morphogenesis; lower concentrations of QDs (1 mg/L) induce an increase of both
nuclear divisions in meiosis in association to an apparently normal progression of spore
morphogenesis. These results indicate that CdS QDs irreversibly induce a severe sporulation
defect in yeast.
C.13
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 109 Poster Communication - Session C
Microencapsulated gallic and ellagic acid as a mean for the biological
control of kiwifruit plants bacterial diseases
M. Sguizzato1,*
, E. Esposito1, A. Rossetti
2, A. Mazzaglia
2, M. Muganu
2, M. Paolocci
2, G. M. Balestra
2,
R. Cortesi1
1Department of Life Sciences and Biotechnology (SVeB), University of Ferrara, Ferrara, Italy.
2Department of Agricultural and Forestry Science (DAFNE), University of Tuscia, Viterbo, Italy.
Over the last decades, kiwifruit cultivation has gained increasing importance all over the
world, but some bacterial diseases caused (for instance) by Pseudomonas syringae pv.
actinidiae Takikawa et al., Pseudomonas syringae pv. syringae van Hall and Pseudomonas
viridiflava (Burkholder) Dowson seriously threaten its cultivation. The diseases control relies
mainly on antibiotics, where allowed, and copper, although this adversely affects the
environment, so that alternative control measures are needed. In the present study gallic acid
and ellagic acid, substances easily obtainable from some plant tissues, were investigated for
their antimicrobial activity aiming to use them as support in the biocontrol of kiwifruit
bacterial diseases. These active principles demonstrated their effectiveness as pure substances
in both in vitro and in vivo tests. Moreover, they were loaded on methacrylate polymeric
microparticles using the spray-drying technique as physical method to their production. Their
encapsulation showed to improve their usefulness and to prolong remarkably their activity up
to 14 days after the treatment, when applied in greenhouse or in field on artificially and
naturally infected plants. The encouraging results obtained by this type of microencapsulated
formulation point the way to future alternative biological control strategies against kiwifruit
bacterial diseases as alternative to, or in combination with a reduced amount of copper
compounds.
C.14
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 110 Poster Communication - Session C
A Calix[4]arene – based DNA topoisomerase I mimic for the promotion of
phosphoryl transfer processes
S. Volpi1, R. Salvio
2, R. Cacciapaglia
2, F. Sansone
1, L. Mandolini
2, A. Casnatia
1
1Dipartimento di Scienze Chimiche, della Vita e della Sostenibilità Ambientale, Università degli Studi di Parma,
Viale delle Scienze 17/A, 43124, Parma, Italy. 2Dipartimento di Chimica e Sezione Meccanismi di Reazione IMC−CNR, Università La Sapienza, P. le Aldo
Moro 5, 00185 Roma, Italy.
The appropriate functionalization of the upper rim of cone-calix[4]arenes has been reported as
an effective strategy for the creation of artificial catalysts able to cleave the phosphodiester
function of nucleic acids and model compounds.1
With the aim to mimic the catalytic triad at the active site of human DNA topoisomerase I,2 it
was synthesized the trifunctional calix[4]arene (1H3)2+
, bearing at the upper rim two
guanidinium units and a phenolic hydroxyl group.3
The diprotonated form of the catalyst (1H2)+ was tested in the cleavage of the DNA model
compound bis(p-nitrophenyl) phosphate (BNPP) in 80% DMSO solution, with rate
enhancement of p nitrophenol liberation, respect to the background hydrolysis, as high as 6.5
× 104-folds at pH 9.5.
According to the experimental data, the three active units cooperate in a reaction sequence
(Figure 1) that involves a phosphoryl transfer process from BNPP to the nucleophilic
phenolate moiety of (1H2)+, followed by the liberation of a second equivalent of p-nitrophenol
from the phosphorylated intermediate, assisted by the neighboring guanidine/guanidinium
catalytic dyad.
Figure 1: Proposed mechanism for the cleavage of BNPP by (1H2)+.
References
1. M. Giuliani, I. Morbioli, F. Sansone, A. Casnati, Chem. Commun. 2015, 51, 14140.
2. M. R. Redinbo, L. Stewart, P. Kuhn, J. J. Champoux, W. G. J. Hol, Science 1998, 279, 1504.
3. R. Salvio, S. Volpi, R. Cacciapaglia, F. Sansone, L. Mandolini, A. Casnati, J. Org. Chem. 2016, 81, 9012.
C.15
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 111 Poster Communication - Session C
Silk fibroin film as a natural material: processing and characterization
D. Vurro1, G. Tarabella
2, P. D’Angelo
1, F. Omenetto
3, L. Ascari
2, S. Iannotta
1*
1IMEM-CNR, Parco Area delle Scienze 37/A 43127 , Parma, Italy.
2Camlin Italy Srl, Strada Budellungo 2 43123 , Parma, Italy.
3Dep. of Biomedical Eng., Tuft University, Medford, Massachusetts 02155, USA.
Bombyx mori silkworm fibroin is a popular protein polymer, nowadays widely used as a high-end
textile fiber and originally used as surgical suture material. The facile processability of the
regenerated form makes it very attractive in a range of biological applications, requiring superior
mechanical properties, biocompatibility, biodegradability and facile-functionalization strategies.
Here, we show the development of a protocol for both an efficient silk fibroin extraction and
optimal thin-film deposition conditions. Our protocol allowed obtaining 8% (w/v) aqueous silk
solution. Two different techniques have been used to make silk fibroin films: solution casting and
spin coating. Freestanding films of 100μm thickness have been prepared by solution casting in a
polystyrene petri dish. XRD analysis has been used to analyze the degree of crystallization of β-
sheet crystallites. Thin films (thicknesses falling in the nm scale) have been obtained by spin
coating technique using different spinning parameters. Film thickness and surface roughness
have been studied by Profilometery and Atomic Force Microscopy, demonstrating that smooth
and thin films can be obtained by combining high spinning speeds and long spinning times.
This preliminary study shows that the properties of high quality fibroin-based films allow their
application as functionalizable substrates for the implementation in electronic devices.
C.16
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 112 Poster Communication - Session C
First determination of fullerenes in the Austrian market and environment:
Quantitative Analysis and assessment
S. Zakaria1,2
, G. Fauler1, E. Fröhlich
*
1Medical University of Graz, Auenbruggerplatz 15, 8010 Graz, Austria.
2Medical University of Graz, Stiftingtalstrasse 24, 8036 Graz, Austria.
This study forms the first report on analyzing fullerenes in the Austrian environment and
cosmetic products available on the Austrian market. We developed, optimized and validated a
novel method for the analysis of C60 and C70 fullerenes and N-methylfulleropyrrolidine C60
(NMFP) for measuring sensitivities in the low ng/L range in order to prove their presence in
the environment (12 wastewater- and 12 sewage sludge samples) and in 11 selected fullerene
containing cosmetic products from three different brands. The optimized method relies on a
liquid-liquid extraction (LLE) or solid-liquid extraction (SLE) and, for the first time,
introduced the Carrez-clarification, followed by liquid chromatography (LC) and coupled to a
hybrid triple quadrupole mass spectrometry (MS) quantification. The total variability of the
new established LC-MS/MS method based on all the tested matrices was below 10%. We
found recoveries generally higher than 70% for both tap water and surface water.
The limits of quantification (LOQ) for the wastewater samples were measured to be from 0.8
to 1.6 ng/L, for the sewage sludge samples, from 1.4 to 2.6 ng/g DM (Drymass) and for the
cosmetic samples from 0.2 to 0.4 ng/g. None of the analyzed samples of wastewater or
sewage sludge samples contained fullerenes. But in 70% of the tested cosmetics, fullerene
concentrations between 10 to 340ng/g were detected. These values were much lower than
concentrations causing toxicity in water animals. It is concluded that there is currently no risk
for environmental toxicity by fullerene exposure in Austria.
C.17
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 114 Poster List
Session A
Technologies, Chemistry and Physics
A.01
M. Basini, D.Peddis, A.Lascialfari
Effect of the hollow topology on the local spin dynamics in Iron Oxide MNPs
A.02 M. Cannio, M. Lassinantti Gualtieri, M. Romagnoli
Stabilization and thermal conductivity of aqueous nanofluids
A.03 E. Carignani, S. Borsacchi, F. Martini, L. Calucci, C. Forte, M. Geppi
Solid state NMR spectroscopy and nuclear spin relaxometry for the investigation of
nanomaterials
A.04 B. Cogliati, A. Arduini, A. Secchi, L. Serravalli, G. Trevisi, P. Frigeri
Synthesis and investigation of croconates as smart organic coating for nobel metals
nanoparticles
A.05 M. Culiolo, M. Villani, D. Delmonte, D. Calestani, N. Coppedè, M. Solzi, L. Marchini, R.
Bercella, A. Zappettini, T.Y. Kim, S.W. Kim
ZnO nanorod-based piezoelectric stress sensor embedded within carbon fiber composite
A.06 A.M. Ferretti, A. Silvestri, M. Marelli, V.Pifferi, L.Falciola, A. Ponti, L.Polito
Synthesis of water dispersible and catalytically active gold-decorated cobalt ferrite
nanoparticles
A.07
C. Fornaini, F. Poli, E. Merigo, S. Selleri, A. Cucinotta
Ultrastructural analysis of dental ceramic surface processed by a 1070 nm fiber laser
A.08 A. Gasparotto, G. Carraro, C. Maccato, D. Barreca
Plasma processing of oxide-based nanomaterials for sensing and energy applications
A.09 M. Takhsha Ghahfarokhi, F. Casoli, S. Fabbrici, R. Cabassi, F. Albertini
Epitaxially grown NiMnGa thin films: the impact of growth conditions on microstructure and
magnetic configuration
A.10 G. Magnani, D. Pontiroli, M. Gaboardi, C. Milanese, G. Bertoni, A. Malcevschi, K.F. Aguey-
Zinsou, M. Riccò
The potentiality of carbon nanostructures for hydrogen storage
A.11 V. Ricci, S. Erokhina, P. D’Angelo, V. Erokhin, S. Iannotta
Nano-engeneered smart filters: towards induced release
A.12 R. Di Corato, A. Aloisi, R. Rinaldi
Superparamagnetic nanoparticles with enhanced magnetic properties: synthesis and
biocompatible coating
A.13 S. Scaravonati, D. Pontiroli, G. Magnani, B. Galante, B. Musig, A. Malcevschi, C. Milanese,
G. Lucchesi, G. Bertoni, M. Riccò
Carbon nanostructures for high-performance supercapacitors
A.14 G. Taormina, C. Sciancalepore, M. Messori, F. Bondioli
Preparation of acrylate-based silver nanocomposite by simultaneous polymerization–reduction
approach via 3D printing technique
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 115 Poster List
A.15 R. Taurino, M. Messori, F. Bondioli
Design and characterization of hydrophobic and oleophobic multilayer films by sol-gel process
A.16 M. Villani, G. Bertoni, F. Fabbri, L. Lazzarini, D. Calestani, N. Coppedè, C. Morasso, S.
Beretta, F. Terenziani, A. Zappettini
Coupling nanostructured metal oxides with gold nanoparticles: a multidisciplinary study
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 116 Poster List
Session B
Medicine, Health, Drugs and Veterinary Medicine
B.01
T. Balbi, R. Fabbri, M. Montagna, G. Camisassi, A. Salis, G. Damonte, L. Canesi
Identification of nanoparticles “protein-coronas” in the blood of marine invertebrates: effects
on the immune response
B.02 M.G. Bianchi, L. Paesano, M. Allegri, M. Chiu, G. Taurino, A. L. Costa, M. Blosi, S. Ortelli,
N. Marmiroli, O. Bussolati, E. Bergamaschi
LPS adsorbed to the bio-corona of TiO2 nanoparticles powerfully activates selected pro-
inflammatory transduction pathways
B.03 M. Boi, M. Bianchi, G. Marchiori, M. Berni, A. Russo, M. Sartori , M.C. Maltarello, F.
Salamanna, G. Giavaresi, M. Fini, M. Marcacci
Nanoindentation of engineered bone tissue: a still unexplored option in regenerative medicine
B.04 C. Cella, F. Mariani, M.A.C. Potenza, P. Milani, T. Sanvito
Enabling development and formulation of Drug Delivery Systems based on micro and
nanoparticles by Single Particle Extinction and Scattering (SPES) technology
B.05 N. Coppedè, M. Giannetto, A. Castellini ,V. Lucchini, L. Ceriani, M. Villani, S. Iannotta, M.
Careri, A. Zappettini
Textile sensors for wearable physiological monitoring
B.06 F. Faroldi, M. Giuliani, S. Fallarini, A. Casnati, F. Compostella, G. Lombardi, F. Sansone
β-N-Acetyl-D-mannosamine-containing calixarenes as multivalent immunostimulators
B.07 B. Ghezzi, P. Lagonegro, L. Parisi, R. Pece, C. Galli, G. Attolini, F. Rossi, G.M. Macaluso, G.
Salviati
3-(Mercaptopropyl)- trimethoxysilane functionalization of scaffolds based on SiOxCy
nanowires for tissue engineering applications
B.08
G. Graziani, G. Carnevale, A. Pisciotta, L. Bertoni, M. Boi, A. Gambardella, M. Berni, G.
Marchiori, A. Russo, A. De Pol, M. Bianchi
Bone-like apatite thin films for bone regeneration: pulsed electron deposition from a biogenic
source
B.09 G. Mandriota, R. Di Corato, R. Rinaldi
Polydopamine-functionalized superparamagnetic nanocrystal clusters as potential magnetic
carriers for biomedical applications
B.10 E. Mazzoni, F. Frontini, I. Bononi, S. Pietrobon, M. Manfrini, G. Guerra, F. Martini, M.
Tognon
Specific IgG antibodies react to mimotopes of BK polyomavirus, a small DNA tumor virus, in
healthy adult sera
B.11 E. Oppici, A. Roncador, M. Talelli, A.N. Pariente, M. Donini, S. Dusi, C. Borri Voltattorni,
M.J. Vicent, B. Cellini
Use of polymer conjugates for the intraperoxisomal delivery of engineered human alanine:
glyoxylate aminotransferase as a protein therapy for primary hyperoxaluria type I
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 117 Poster List
B.12 D. Orsi, T. Rimoldi, S. Pinelli, R. Alinovi, G. Benecchi, F. Rossi, L. Cristofolini
CeF3 – ZnO nanostructures for the Self-lighted Photodynamic Therapy of deep tumors
B.13 L. Paesano, A. Perotti, A. Buschini, C. Carubbi, M. Marmiroli, E. Maestri, S. Iannotta, N.
Marmiroli
Markers for toxicity to HepG2 exposed to cadmium sulphide quantum dots; damage to
mitochondria
B.14 I. Rossi, E. Quarta, F. Sonvico, F. Buttini
Nebulized coenzyme Q10 nanosuspensions: a versatile approach for pulmonary antioxidant
therapy
B.15 A. Rozzi, J. Brintinger, K. Shabnam, P. Rudatis, N. Yevalik, Z. Valeriia, W. Knoll, R. Corradini
Liquid-gated field-effect transistors based on reduced graphene oxides as sensing platform for
biomolecules
B.16 A. Sargenti, S. Iotti, C. Cappadone, G. Farruggia, A. Procopio, E. Malucelli
Ultrastructural study of biomineralization process in human bone marrow mesenchymal stem
cells during the osteoblastic differentiation
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 118 Poster List
Session C
Agrifood, Environment and Biotechnologies
C.01
S. Argentiere, C. Cella, C. Lenardi
Estimating consumer exposure to silver nanoparticles from food contact materials: the
study of migration properties
C.02 D. Beconcini, R. Berni, F. Felice, M. Romi, A. Fabiano, Y. Zambito, R. Di Stefano, T. Santoni,
G. Cai, C. Cantini
Characterization of nutritional and health properties of local Tuscany cherry varieties
and evaluation of innovative nanosystems delivery
C.03 L. Bergamonti, F. Bondioli, C. Graiff, A. Haghighi, C. Isca, P.P. Lottici, B. Pizzo, G. Predieri
Preparation of cellulose nanocrystals for decayed old wood consolidation
C.04 F. Bianchi, M. Mattarozzi, D. Catellani, M. Suman, M. Careri
From field to fork: determination of metal nanoparticles in the pasta food chain
C.05 M. Caldara, C. Agrimonti, A. Zappetini, N. Marmiroli
Characterization of mutants tolerant to cadmium sulphide quantum dots
C.06 E. Falletta, C. Della Pina
Nanoporous polyanilines for pollutants removal from air and wastewater
C.07 V. Gallo, M. Marmiroli, V. Srivastava, N. Marmiroli
Proteomic analysis of CdS QDs response in Saccharomyces cerevisiae
C.08
D. Imperiale, M. Marmiroli, L. Pagano, M. Villani, A. Zappettini, N. Marmiroli
The proteomic response of Arabidopsis thaliana to cadmium sulfide Quantum Dots
and its correlation with the transcriptomic response
C.09 D. Lizzi, A. Mattiello, L. Marchiol
Germination of common velvet grass (Holcus lanatus L.) and dandelion (Taraxacum
officinale F. H. Wigg) as affected by nCeO2 of different size
C.10 F. Mussi, D. Imperiale, G. Lencioni, A. Zappettini, M. Villani, N. Marmiroli, M. Marmiroli
CdS QDs and CdSO4: oxidative stress-induced toxicity in A. thaliana w.t.
C.11 F. Pasquali, C. Agrimonti, L. Pagano, A. Zappettini, M. Villani, M. Marmiroli, J.C. White, N.
Marmiroli
Nucleo-mitochondrial interaction of yeast in response to cadmium sulfide quantum dot
exposure
C.12 G. Pira, M. Villani, A. Zappettini, N. Marmiroli, R. Ruotolo
Ring-shaped proteins bind nanoparticles and affect their bioactivity
C.13 R. Rossi, M. Villani, A. Zappettini, N. Marmiroli, R. Ruotolo
CdS QDs induce mutagenesis and affect spore morphogenesis in Saccharomyces
cerevisiae
3rd “PARMA” NANO-DAY, July 12-14, 2017
ISBN 978-88-941066-8-8 119 Poster List
C.14 M. Sguizzato, E. Esposito, A. Rossetti, A. Mazzaglia, M. Muganu, M. Paolocci, G.M. Balestra,
R. Cortesi
Microencapsulated gallic and ellagic acid as a mean for the biological control of
kiwifruit plants bacterial diseases
C.15 S. Volpi, R. Salvio, R. Cacciapaglia, F. Sansone, L. Mandolini, A. Casnatia
A Calix[4]arene – based DNA topoisomerase I mimic for the promotion of phosphoryl
transfer processes
C.16 D. Vurro, G. Tarabella, P. D’Angelo, F. Omenetto, L. Ascari, S. Iannotta
Silk fibroin film as a natural material: processing and characterization
C.17 S. Zakaria, G. Fauler, E. Fröhlich
First determination of fullerenes in the Austrian market and environment: Quantitative
Analysis and assessment
The organizers acknowledge the contributes of the following institutions:
- University of Parma
- IMEM-CNR, Parma
- INSTM, Firenze
- Consorzio Italbiotec, Milano
- CINSA, Parma
- Camera di Commercio di Parma
- Fondazione Cariparma
- Collegio Europeo, Parma
- Unione Parmense degli Industriali
University of Parma has provided the resources for conference book and for the Poster
Awards. Consorzio Italbiotec and IMEM-CNR granted the coffee break. Fondazione
Cariparma and Camera di Commercio di Parma both contributed to the Poster Awards.
CINSA and INSTM contributed actively to cover general expenses of the conference.
Acknowledgement
Patronage