39 Matthew Blair Tli Objective3 Phase Ii Work Plan
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Transcript of 39 Matthew Blair Tli Objective3 Phase Ii Work Plan
TL1 proposal: common bean
TL2 annual Meeting, Bamako Mali – Nov. 2009
(Objective 3 – Tropical Legumes I)
Objective 3 Common bean – Major Outcome flows to TL-1 Phase II
Germplasm Screening
Genomic Resources
Marker Associations
Population development
SSR platform (1500) from EST, BAC, etc., and
genotyping sets 1st gen. SNP platform (768)
Germplasm genotyped
Germplasm phenotyped (604) drought tolerance
Sources of drought tolerance
Diversity Sets: Ethiopia, Kenya,
ECABREN / SABRN and
Reference Coll.
Deep rooting sources
Mapped markers / Physical map
QTL for deep rooting and for carbohydrate
translocation
Marker-assisted backcrossing to develop improved
local varieties
MAS crosses (CBB, BCMV, Bruchid)
Adv Backross populations created (5)
Early generation populations and advanced lines
Adv. Backcross and MARS breeding populations for
Andean drought tolerance
2007
2010
2014
Phase I Phase II
Activity
Tools
Operation
Outcome
SNP confirmation
For Intra-genepool populations
QTL populations phenotyped (deep
rooting and carbohyd. translocation)
Intra-genepool Elite x Elite Crosses (40)
Inter-genepool lines (DAB and CBIB lines)
Advanced lines and segregating
populations
Act. 1: Evaluate rooting depth of parental sources
• Extending work on reference and regional collections (G. Makunde, Ph.D. thesis)
Act. 2: Validate SNPs for inter- and intra-gene pool crosses
• For 50 donor and recipient genotypes
• For introgression to Andeans
• For use in MARS
Andeans
Mesoamericans
Act. 3: Deploy insect and disease resistance genes
• Confirm MAS in advanced lines – BCMNV – Storage insects – Bacterial Blight
• RGA for Bacterial blight
Act. 4: Deploy drought tolerance traits from
inter-gene pool crosses in Andean
gene pool
• Characterize traits in 40 parentals
• Develop & test visual scales
• QTL for Pod Harvest Index & deep rooting
• Test 60 Andeans for heat tolerance
ooooo
Pod Partitioning
Index
Pod Harvest Index
Act. 5: Pyramid drought tolerance traits through MARS and Advanced Backcross
in Andean gene pool • Genotype 2 AB
populations
• Genotype & phenotype 4 RIL populations for MARS
• 3 more populations for MARS
• Pyramiding from AB and RILs
P1 P2
F1
F1-BC1
F1-BC2
X
Advanced Backcross Lines
AdvancedBackcrossScheme
QTL identification
Phenotype
Genotype Genetic Map
Mesoamerican Drought Tolerance
Sources SER16 SER48
Microsatellites and SNPs Andean
Red Mottled Cultivars
CAL96 (Uga) CAL143 (Mwi)
2 AB-QTL Populations
selected
Line distribution
F2: Drought Single Plant Selection Lowland site
F3: Plant select. Low P + MAS for R gene Highland site
F3.4: Families Lowland site
F1 to F2
F3.5: Drought Multi-location trials option
Phenotyping for QTL analysis Double & Multiple
crosses
DNA extraction
Genotyping
QTL analysis
F1: Simple cross Drought tol. X And. commercial
Lines Derived and Recombination Cycles
MarkerAssistedRecurrentSelectionScheme
Cycle 1 starts
Act. 6: Training of students in drought tolerance and MAS
• Training students in MARS • Short course in CIAT
Act. 7: Data storage and management
Proposed Act. 8: Testing the out-sourcing of marker analysis with partners
• Samples currently cost $8 in Africa (paid by special project using unique method)
• Outsourcing would cost $2-3
• Accuracy of markers would be verified – Bacterial blight – Storage insects