30504344 Environmental Microscopy Power Point
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Transcript of 30504344 Environmental Microscopy Power Point
8/8/2019 30504344 Environmental Microscopy Power Point
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Lab 5
Microscopy
Lab Manual pages 71-85
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What is Microscopy?
Microscopy: the technical field of usingmicroscopes to view samples or objects.
Very important tool in science
which led to many scientific
discoveries.
Anton van Leeuwenhoek, 16th
century viewer of bacteria
Robert Hooke, first person to
coin the term cell
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Types of Microscopes
Optical/LightMicroscopes, which include compound anddissecting/stereo
microscopes
Uses light and lenses tomagnify images
Simple vs. CompoundScopes
Dissecting Compound
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Types of Microscopes
Non-optical microscope
Scanning ElectronMicroscope
(SEM)/TransmissionElectron Microscope (TEM)
Produce images byscanning the sample with a
high energy beam of electrons
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How TheyWorkOptical
Microscope Non-optical
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Optical vs. Non-optical
Characteristics Optical Non-optical
Magnification 40-1200X 25-250,000X
Possible Samples Can be living or deadMu
st be trimmed andcoated/fixed
Resolution Moderate Very High
Benefits Less expensive Very wide depth of
field (3D Images)
Limitations Limited Depth of field Very Expensive andmore difficult to
operate
Depth of field: vertical distance that remains in focus at one time
(decreases as magnification increases in light microscopes)
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Parts of the Light Microscope
Ocular
Eyepiece,closest piece to
the eye
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Parts of the Light Microscope
Ocular
The lens ormirror of a
microscope
Objective
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Parts of the Light Microscope
Ocular
Platform where
slides are
placed (can beadjusted up or
down)
Objective
Stage
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Parts of the Light Microscope
Ocular
Holds the slidesin place for
viewing
Objective
Stage
Stage Controls
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Parts of the Light Microscope
Ocular
Lens thatfocuses light on
the specimen
Objective
Stage
Stage Controls
Condenser
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Parts of the Light Microscope
Ocular
Function is to stopthe flow of light
(can be adjusted
left or righ
t)
Objective
Stage
Stage Controls
Condenser
Diaphragm
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Parts of the Light Microscope
Ocular
Controls thestrength of the
light being used
Objective
Stage
Stage Controls
Condenser
Diaphragm
Light Intensity Control
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Parts of the Light Microscope
Ocular
Used to focus inon specific
features of the
sample
Objective
Stage
Stage Controls
Condenser
Diaphragm
Light Intensity Control
Coarse
Focus Knob
Fine
Focus Knob
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Parts of the Light Microscope
Ocular
Used to powerthe microscope
on and off
Objective
Stage
Stage Controls
Condenser
Diaphragm
Light Intensity Control
Coarse
Focus Knob
Fine
Focus Knob
Power
On/Off
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Magnification
Magnify/Magnificaton: to enlarge something in
appearance.
3 Objectives:
1. Scanning (4X)
2. Low (10X)
3. High (40X)The ocular lens (eyepiece) is 10X
Total magnification= objective x ocular
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Magnification
Magnify/Magnificaton: to enlarge something in
appearance.
3 Objectives:
1. Scanning (4X)
2. Low (10X)
3. High (40X)The ocular lens (eyepiece) is 10X
Total magnification= objective x ocular
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Magnification
Magnify/Magnificaton: to enlarge something in
appearance.
3 Objectives:
1. Scanning (4X)
2. Low (10X)
3. High (40X)The ocular lens (eyepiece) is 10X
Total magnification= objective x ocular
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Staining
Stains are used in microscopy to increase contrast or color in
specific components of a specimen
Stained Red Blood CellsGram Positive Bacteria:
streptococcus pneumoniae
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Clonorchissinensis
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Mosquito
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Lice
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Handling of Microscope
Use lens paper ONLY to clean microscope
lenses
StartA
LL observations on th
e lowestpowered objective (4X)
Only use coarse focus on the low power
(10X) objective and fine focus on high
power (40X) objective
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Lab Materials and Trouble Shooting
Microscope Slides Cover Slips
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Todays Lab
PART A:
± Table 1, pg.73
± e slide (pgs. 74-75);
± # 12, (Desmids OR Rizophus) ± PreparingWet Mount : 1 drop of Chlorella algae (pg. 76)
± Using a Biological Stain (78): WARNING:Calberla willstain!!!; gloves can be worn if needed; 2 drops of pollen onslide, but only stain 1; during the 5 min it takes to stain you
can start on Part B ± Table 2, pg. 79
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Todays Lab
PART B: The Dissecting Microscope
± Figure 1
± e slide
± Pg. 71; Total of 4 specimens: Duckweed, Peacock
feather, Beetle, or Printed Photo (view and draw
only 1 specimen)
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Todays Lab
Part C: The Electron Microscope (81)
± Examine the photomicrographs in the packet, to
fill in specimens for Table 3.
± For questions below Table 3, use
photomicrograph (Cat Flea)
± All of this must be checked off before you leavetoday.
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Todays Lab
PART D: Clean Up (pg. 74)
± Wash hands
± TU RN OFF ALL SCOPES AND BE SU RE TH EY ARE SE T T OTH E SCANNING (LOWEST) OBJEC T IVE
± Clean all microscope stages
± Rinse all slides with DI water
± Used cover slips go into the Broken Glass Container (NOTIN THE TRASH)
± Transfer pipets can be trashed
± Clean cubbies and throw away any other trash
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HOMEWORK
Work on Allelopathy Lab Reports
Read Lichens and the Environment Lab.
Remember Next Week IS AN OUT SIDE LAB