Speed & Flexibility: The ADDA System

Nick LevittCPSA USA October 3, 2012

• Cheetahs• Race cars• Jet planes…

What is high speed?What is high speed?

Designs that make sense 2

The ADDA AutosamplerThe ADDA Autosampler

Designs that make sense 3

• Online SPE in 8 seconds

• Injection to injection timing

• One line equilibrates while the other performs

• Washing (internal and external rinse) for low carryover

What is flexible?What is flexible?

Designs that make sense 4

• Some Buddhist monks

• Fold-up Cars (2013)…

The ADDA AutosamplerThe ADDA Autosampler

Designs that make sense 5

• Automatically switch between:• High-speed trap & elute

mode• Full gradient mode.

• Combine high-speed screening with in-depth sample analysis• Separating interferences• Quan/Qual workflows• Flexibility for the discovery

lab

IntegratedIntegrated• Software designed by Sound Analytics LLC• Fully integrated with AB SCIEX mass

spectrometers

Designs that make sense 6

Dual stream LC-20AD (Shimadzu) gradient trace. ADDA 75s injection to injection, flow rate = 0.5ml/min. Baseline separation of five standard CYP450 inhibition metabolites (8 samples, 10 minutes)

1A2

2D6

3A4

2C9

2C8

FlexibleFlexible

8Designs that make sense

The ADDA Dual Arm Autosampling System.

SpeedFlexibility

From Apricot Designs.

For more information, contact:

Nick Levitt nick@twocenter.comJeremy Lawton jlawton@apricotdesigns.com

Designs that make sense 9

• The speed of 2 high speed robotic arms• Online SPE LC/MS at 8s /sample with wash.• A software system to control all valves, pumps, and the MS.

The ADDA Autosampling SystemThe ADDA Autosampling System

RuggedRugged• Millions of samples, minimal downtime• Factory style instrumentation for easy maintenance

access and maximum uptime.• Standard parts where applicable for solid operation.

Designs that make sense 10