3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN...
Transcript of 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN...
![Page 1: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/1.jpg)
3. Prelab
Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17
BOSTON UNIVERSITYDRESDEN SCIENCE PROGRAM
ORGANIC CHEMISTRY LABORATORY CAS CH 203, FALL 2014
![Page 2: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/2.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach
![Page 3: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/3.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach
Chloroplasts contain colored compounds (pigments) which fall into two categorieschlorophylls and carotenoids
![Page 4: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/4.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach
Chlorophyll b: -CHO group instead of shaded methyl groupPheophytin a: identical to chlorophyll a except that Mg2+ is replaced by two H+
Pheophytin b: identical to chlorophyll b except that Mg2+ is replaced by two H+
![Page 5: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/5.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach
![Page 6: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/6.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach
![Page 7: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/7.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach
-Carotene
-Carotene
-Carotene
Different position of the double bond
Xanthophylls are oxidized derivatives of carotenes. They contain hydroxyl groups and are more polar.
![Page 8: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/8.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach
Carotene is responsible for the orange colour of the carrots and many other fruits and vegetables.Xanthophylls are oxidized derivatives of carotenes. They contain hydroxyl groups and are more polar.
![Page 9: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/9.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach
![Page 10: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/10.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach
![Page 11: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/11.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography
![Page 12: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/12.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography
![Page 13: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/13.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography
![Page 14: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/14.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography
![Page 15: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/15.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography
![Page 16: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/16.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography
![Page 17: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/17.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography
![Page 18: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/18.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography
![Page 19: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/19.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography
![Page 20: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/20.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography
![Page 21: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/21.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography
![Page 22: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/22.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography
![Page 23: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/23.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography
![Page 24: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/24.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 14 – Thin-Layer Chromatography
![Page 25: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/25.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 14 – Thin-Layer Chromatography
![Page 26: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/26.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 14 – Thin-Layer Chromatography
![Page 27: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/27.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 14 – Thin-Layer Chromatography
![Page 28: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/28.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 14 – Thin-Layer Chromatography
![Page 29: 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203,](https://reader035.fdocuments.us/reader035/viewer/2022062518/56649ca45503460f949650ad/html5/thumbnails/29.jpg)
Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 14 – Thin-Layer Chromatography