2424 Evaporative Light Scattering DetectorPutting confidence in your detection strategy

Benefits of adding ELS detection ■■ Orthogonal detection

technique

■■ Detection of analytes

without UV/Vis

chromophore and/or do

not ionize well in MS

■■ Nanogram-level

detection limits

■■ Mass response

independent of

analyte’s optical

properties

Waters 2424 ELS Detector.

The 2424 ELS Detector allows you to reproducibly detect semi-volatile components as demonstrated by this chromatographic test mix of semi-volatile and non-volatile components. Excellent detection is achieved across the range of components without interference from the DMSO sample diluent.

Caffe

ine

Naph

azol

ine

Oxy

met

azol

ine

Oxy

buty

nin

Nabu

met

one

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ACHIEV E RICHER ANALYSIS

The Waters® 2424 Evaporative Light Scattering (ELS) Detector, with its

compact and innovative design, offers near universal detection of non-

volatile and semi-volatile sample components. The 2424 ELS Detector is

compatible with both isocratic and gradient separations and has nebulizer

options to cover a wide range of analytical and microbore flow rates. By adding

the orthogonality of ELS to your detection strategy, you increase peak detection

confidence across a wide application space. The 2424 ELS Detector can be

used as part of an Alliance® HPLC or LC/MS system under the control of either

Empower® or MassLynx® software, or can be operated as a stand-alone detector.

Quantification for your entire sample

Finding the right balance in a detector designed for evaporative light scattering

detection can be the difference between good sensitivity of semi-volatile

components and high peak dispersion. The 2424 ELS Detector was designed

with this critical balance in mind and delivers reproducible quantification for

semi-volatile components without adding high dispersion to fast LC separations.

Sample components that elute in the column void volume can be difficult to

detect with many techniques, creating an issue of particular concern for strong

UV absorbing diluents such as DMSO. The desolvation characteristics of the

2424 ELS Detector can remove the sample diluent interference to give visibility

to unretained components in your sample.

AP PLICAT ION SOLUT IONS FOR T HE LABORATORY

Laboratories around the world rely on the proven performance

of Waters ELS detectors for superior results across a vast range

of applications, including the analysis of carbohydrates, fatty

acids, lipids, surfactants, and pharmaceuticals.

Complex applications with gradient analysis

Whether trying to monitor a complex process such as the

consistent production of food oils or verifying their authenticity

to prevent adulteration, the use of gradient analysis is

important for providing a wider separation range of your

samples. The 2424 ELS Detector is entirely compatible with

gradient analysis, and with significantly reduced stabilization

times as compared to other ELS detectors, you can get

consistent answers, faster.

Enhance your existing detection capabilities

For applications where not all components have a UV

chromophore, adding ELS detection expands the visibility

of your sample by providing a complementary

detection capability.

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Molasses

Food sugar standard1 2 3

45

12

31. p-Toluamide 2. Fructose 3. Glucose4. Sucrose 5. Maltose 6. Lactose

The gradient analysis of triglycerides in edible oils, overlay of safflower oil (blue) and olive oil (green).

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ELS

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me-

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harin

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harin

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alos

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arta

ne

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espe

ridin

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arta

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Neoh

espe

ridin

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AU

LSU

While some artificial sweeteners can be analyzed by UV, several lack UV chromophores. In this example, all these artificial sweeteners can be quantified by adding ELS detection.

Simple food sugars lack a UV chromophore, but they are easily detected and quantified in complex matrices using the 2424 ELS Detector.

ALL T HIS T ECHNOLOGY, WIT HOUT ALL T HE BENCHSPAC E

The 2424 ELS Detector hosts a multitude of innovative design features for detection of your non-UV samples, but with

simple integration into your system and your laboratory environment.

Designed for your laboratory■■ Compact footprint for system stacking and reduced

laboratory space requirement

■■ Front-mounted, user-installable lamp

■■ Easily accessible nebulizers covering wide flow rate range

■■ Open access ready with accelerated nebulizer stabilization

Maximize your method performance■■ Sampling rates up to 80 points/sec

■■ Independent optimization of digital filtering

■■ Active nebulizer heating and cooling

■■ Auto-optimize gain function

■■ Optional external column heater control

Detection technology■ Reference energy monitoring for consistent long-term signal■ 60° scatter angle detection to minimize stray light and optics polarization

for long-term optimal sensitivity■ Normalized energy output for improved bench-to-bench repeatability

Nebulization technology■ Concentric flow nebulizer designed

for consistent droplet formation■ Heating for method optimization■ Cooling for rapid stabilization

Desolvation technology■ Temperature controlled and

monitored to provide optimal conditions for desolvating semi-volatile components

■ Optimized geometry for analytical HPLC peak volumes and flow rates

■ Desolvation of interfering sample diluent peaks to expose unretained sample components

Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com

Waters, Empower, MassLynx, and Alliance are registered trademarks of Waters Corporation. SystemsQT and T he Science of What’s Possible are trademarks of Waters Corporation. All other trademarks are the property of their respective owners.

©2013 Waters Corporation. Printed in the U.S.A.February 2013 720004525EN TC-PC

COMPLET E T HE CHROMATOGRAPHIC P ICTURE AND BUILD HIGHER CONFIDENC E IN YOUR RESULT S

Pair a 2424 ELS Detector with a Waters 2489 UV/Vis Detector and SQ Detector 2

single quadrupole mass detector, and achieve the capability to:

■■ Derive more complete peak information in one chromatographic run,

■■ Detect molecules that lack a UV/Vis chromophore, complementing absorbance

detector information, and

■■ Analyze compounds that don’t ionize well by MS.

Supported in both Empower and Masslynx software, you can select the right

chromatographic data system for your laboratory’s needs. The 2424 ELS Detector

is also compliance-ready as part of the Alliance HPLC family of systems supported

within the Systems Qualification Tool (SystemsQT™) technology.

Alliance HPLC System with 2424 ELS Detector, 2998 PDA Detector, and SQ Detector 2.

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