22.09.2008 Content: Overview about project Genetic survey Why? Results Conclusions for...

22.09.2008 www.life-bombina.de

Content: Overview about

project Genetic survey

Why? Results Conclusions for

management Project activities in

Denmark

Fire bellied toad, Klokkefrø, Rotbauchunke

(Bombina bombina)

Management von Rotbauchunken- Populationen im Ostseeraum


Project structure

Beneficiary: Stiftung Naturschutz:10 sitesPartner:

• AMPHI Consult: expertise for habitat and population management, assistant management for Denmark and Sweden

• Denmark: DFNS (for former partner Fyns Amt, Storstrøms Amt, Vestsjællands Amt: 11 sites

• Sweden: Nature: 4 sites (for genetic survey)• Lettland: Latgales Ekologiska Biedriba: 1 site, Amphi

Consult 1 site• LANU SH genetic survey• Zoo Copenhagen: capativity population of Hjordø

Cofinancer:• Klara-Samariter-Stiftung• Nature protection board - Latvia• WWF Denmark• Vordingborg Commune

Duration: April 2005 to December 2010

LIFE-Bombina overview


Project sitesLIFE-Bombina overview


Situation of B. bombina

Similar threats at the northern edge of the distribution range of this south-european specie: often small, isolated populations Several populations at the edge

of extinction in the beginning of the project

Similar problems caused by intensification of land use or abandonce of traditional grazing regimes at the breeding ponds

Fish release to breeding ponds



Improvement of state of conservation of fire bellied toad populations: increase of calling males in the projetc sites by:

Experience exchange Habitat management Population management Genetic analysis for securing the

population management actions Rising of public awareness

Project objective and actions



Conservation strategie

Increase of suitable breeding habitats for B. bombina (Amphi Consult experience)

Stabilize small populations by supportive breeding (Amphi Consult experience)

Organising suitable land management around the ponds (Schleswig-Holstein experience)

Establish new mirror and mixed populations, the sucessfull concept of the former Danish Bombina LIFE-project



Increase of pond habitatLIFE-Bombina overview

over 130 ponds dug or restored by the LIFE Bombina project

Spin off effect in SH: 350 ponds additional dug by ELER funding from ministry


Population managementLIFE-Bombina overview


Aufzuchtstation in Kiel

Population managementLIFE-Bombina overview

Over 15.000 eggs reared and over 12.000 young Bombina released (until 2007)

Increasing numbers of calling males at nearly all sites


GrazingLIFE-Bombina overview

per ha: approx. 0,5 to 0,6 animal units

Pond bank length per animal unit: 70 m


Conservation strategy of mirror populations successfull?

Which populations are autochton in Schleswig-Holstein?

Inbreeding? Identify management units Mixed Danish populations in

Sweden more successfull?

Why Genetic survey?Genetics

Captivity population of island Fehmarn, Michael Løgstrup, Amphi Consult

breeding and rearing cage for outdoor use


Mito-DNA-DataGenetic: results


Distribution of Haplotyps-1

Genetic: results



Genetic: results


Mikro-sattelitesGenetic: results

0,1

DK-NS

DK-ROE

DK-AG

DK-ASDK-ENDK-KO

DK-KS

S-MF

UA

D-LAK

PL-BILV-BA

LI

LV-DP

LV-RZD-SA

D-RAD-HS

D-PU

D-FE

D-SB

D-DW

D-KUE

D-SC

D-GL

D-ERDK-HJ

DK-BI

DK-KH

DK-AV

DK-SK

DK-AE

S-BD

S-FB

DK-TSDK-KL

* Bootstrap Value < 25%Nei’s genetic distance10.000 Bootstraps

91

544361 74

43

80

*

8525 47

94

7661

95

39

3734

41

*

*28*

*

100

10065

6354

3947

75

56

Genetic Structure (microsatellite DNA)Genetic Structure (microsatellite DNA)

S-SH


Results

Illegal releases identified in Sweden and Schleswig-Holstein

Origin of released animals from the pannonian region, lake Neusiedel proofed

Clear seperation of populations between Fyn and Sealand

Genetic drift stronger then expected, proofed by data from Avernakø and Korshavn

Genetic: results


General conclusions

Mirror population concept successfull Management units defined New: Creation of mixed populations at new sites Genetic drift strong process in small populations

Minimum effective population size of 1000 (Ne) is needed. Ne always smaller the real populations size, therefore 2000 to 4000 adult animals are needed per population.

20 m² pond habitat per individual: 40.000 bis 80.000 m² pond surface per population are necessary

Establish meta-populations by connecting sites

Genetics


Management units in SHgeneral conclusions


Meta-Populationskonzept

general conclusions


pond clusters for core populations

general conclusions


PR workLIFE-Bombina


BOMBINA SONG CONTEST

www.life-bombina.de

Lauschen Sie den Gesängen der Rotbauchunken-Männchen in Schleswig-Holstein.

Per Internet-Schaltung hören Sie auch die Chöre in Dänemark, Schweden und Estland.

LIVE: Freitag, 11. Mai 2007, 17 bis 19 UhrTreffpunkt: Feuerwehrhaus bei Kühren/Preetz.

Und von überall zu hören unter:

Bombina calling 2007

Machen Sie mit! Wählen Sie Ihren Unken-Starchor!Füllen Sie den Wahlzettel aus und geben ihn ab.Die Teilnehmer:

Klintholm-Allstars– Dänemark

Mölle Tenöre – Schweden

Islice-Chor – Lettland

Feuerbauch-Tenöre – Deutschland

Live Mitschnitteaus den anderen Gebieten werden ab 18 Uhrpräsentiert.

Sie können ein Paket Wurstwaren von „Gallowaysaus Wilden Weiden“der Stiftung Naturschutz gewinnen. Verlosung um 19 Uhr.

Konzertmitschnitt unter:

1. European-Bombina-Song-Contest• Latvia choir was forced back by late snow

• winner: Sweden

• Many articles

• Good coverage by TV and Radio

• DK Radio commenting the „Eurovision Songcontest“ two weeks later: „Much nicer was the 1. European Bombina Song Contest two weeks ago: Fun was the main aspect of the event.“

• Thank you for attention!

www.life-bombina.de

LIFE-Bombina - PR

22.09.2008 Content: Overview about project Genetic survey Why? Results Conclusions for...

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