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![Page 1: 22-1 Principles and Applications of Inorganic, Organic, and Biological Chemistry Denniston, Topping, and Caret 4th ed Chapter 22 Copyright © The McGraw-Hill.](https://reader036.fdocuments.us/reader036/viewer/2022062315/5697bf931a28abf838c8fc13/html5/thumbnails/1.jpg)
22-1
Principles and Applications ofInorganic, Organic, and Biological
ChemistryDenniston, Topping, and Caret
4th ed
Chapter 22
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
Power Point to Accompany
![Page 2: 22-1 Principles and Applications of Inorganic, Organic, and Biological Chemistry Denniston, Topping, and Caret 4th ed Chapter 22 Copyright © The McGraw-Hill.](https://reader036.fdocuments.us/reader036/viewer/2022062315/5697bf931a28abf838c8fc13/html5/thumbnails/2.jpg)
22-2
22.1 The Mitochondria
Size of a bacterial cell.
Outer mitochondrial membrane.
Inner mitochondrial membrane:
Highly folded membranes=christae
Has electron transport system and ATP synthase
Space between membranes is the intermembrane space.
Interior is the matrix
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22-3
22.2 Pyruvate to Acetyl CoA
Pyruvate from glycolysis enters the mitochondria and is converted to acetyl CoA which activates the acetyl group for entry into the tricarboxylic acid cycle (Kreb’s cycle or TCA cycle).
Pyruvate to acetyl CoA
Decarboxylation (loss of CO2)
Oxidation by NAD+ which accepts the hydride anion.
Acetyl group linked to coenzyme A via a thioester bond.
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22-4
Pyruvate Decarboxylate Complex
pyruvate
Acetyl CoA
VitaminsThiamine PP (B1)
FAD (B2)
NAD+ (niacin)CoA (panthothenic acid)+ coenzyme lipoamide
CH3CO
COO-
CH3CO
S CoA
CoA-SH
NAD+CO2
NADH + H+
TPP, FADMg2+, lipoic acid
![Page 5: 22-1 Principles and Applications of Inorganic, Organic, and Biological Chemistry Denniston, Topping, and Caret 4th ed Chapter 22 Copyright © The McGraw-Hill.](https://reader036.fdocuments.us/reader036/viewer/2022062315/5697bf931a28abf838c8fc13/html5/thumbnails/5.jpg)
22-5
22.3 Overview: Aerobic RespirationOxygen-requiring breakdown of food and
production of ATP.
Enzymes in the mitochondrial matrix.
Three oxidations transfer hydride to NAD+ or FAD.
Electrons passed from NAD+ or FAD to the electron transport chain and then O2.
Protons are transferred to the intermembrane space which leads to the synthesis of ATP as protons return to the matrix.
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22-6
22.4 The Citric Acid CycleAcetyl CoA and oxaloacetate feed the citric
acid (TCA) cycle.The acetyl group is oxidized to two molecules
of CO2 and high energy electrons are transferred to NAD+ and FAD.
CH3 C
O
S O P
O
O
O
O P
O
O
O
NCCH
C
N
NC
CH
N
NH2
P
O
O
OO
CH2
HH
OH
HH
CH2C
CH3
CH3
C
OH
H
C
O
NCH2CH2C
O
NCH2CH2
H H
Acetyl CoA
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22-7
TCA Cycle
SCoACH3CO
CH2CH2C
COO-
COO-
O
CO2
NAD+
CoA-SH
CH2COHCH2
COO-
COO-
COO-
CH2CHC
COO-
COO-
COO-
OH H
CO2
CH2CH2C
COO-
OS-CoA
NAD+
CH2
C O
COO-
COO-
NAD+
CH2CH2
COO-
COO-
GTP Pi
C
-OOC
COO-H
H
FAD
H2O
CH2
C
COO-
COO-
OH H
Nowrecycle
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22-8
The TCA Cycle-1, 2
Citratesynthase
CH2
C
COO-
COO-
O
SCoACH3CO H2O
CH2COHCH2
COO-
COO-
COO-+HSCoA+ H+
Citrate
aconitase
COH
CH2CH
COO-
COO-
COO-
H
Isocitrate
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22-9
The TCA Cycle-3, 4
Isocitrate
isocitrate dehydrogenaseCH2
CC
COO-
COO-
COO-
O HH
H+ NAD+
-ketoglutarate
+ CO2
+ NADH
CH2CH2C
COO-
COO-
O1. Oxidation: ROH to C=O2. Carbon dioxide released3. NAD+ reduced
CH2CH2C
COO-
COO-
O
+ NAD+
+ CoA
-ketoglutarate dehydrogenase complex
CH2CH2C
COO-
OS CoA
+ CO2
+ NADH
Succinyl CoA
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22-10
The TCA Cycle-5, 6
+ GDP + Pi
succinyl-CoA synthase
Succinyl CoA
CH2CH2C
COO-
OS CoA
+ GTP+ CoA
Succinate
CH2CH2COO
-
COO-
CH2CH2COO
-
COO-
+ FAD
succinate dehydrogenase
C
C
-OOC
COO-
H
H
Fumarate+ FADH2
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22-11
The TCA Cycle-7, 8
+ H2Ofumarase
C
C
-OOC
COO-H
H L-Malate
CC
COO-
COO-
OH HH H
CH2
C
COO-
COO-
O
malate dehydrogenase
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22-12
22.5 Control of the TCA Cycle
Insert Fig 22.6 to fill below line
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22-13
22.6 Oxidative PhosphorylationThe respiratory electron transport system is
made up of a series of electron carriers embeded in the inner mitochondrial membrane.
At three sites in the electron transport system, protons (H+) can be pumped from the matrix to the intermembrane space.
NADH provides three ATP molecules
FADH2 provides two ATP molecules
because only two sites are involved.
ATP synthesis occurs at the ATP synthase.
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22-14
Insert Fig 22.7 4th edI want to add comments after the
Figure is in.
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22-15
ATP Synthase and ATPThe terminal electron acceptor in the
transport chain is dioxygen.
½ O2 + 2 H+ H2O
The protons in the intermembrane space flow back through ATP synthase F0 channel
activating F1 that catalyzes phosphorylation
of ADP to produce ATP.
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22-16
Energy Yield from One GlucoseGlycolysis
Substrate-level phosphorylation 2 ATP
2 NADH X 2 ATP (cytoplasm) 4 ATP
Two Pyruvate to two Ac-CoA
2 NADH X 3 ATP/NADH 6 ATP
Citric Acid Cycle (Two turns)
2 GTP X 1 ATP/GTP 2 ATP
6 NADH X 3 ATP/NADH 18 ATP
2 FADH2 X 2 ATP/FADH2 4 ATP
NET 36 ATP
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22-17
22.7 Degradaton of Amino AcidsWhen the body has depleted glycogen
(starving), it can use amino acids for fuel.
Degredation takes place in the liver in two stages.
1. Removal of the amino group.
2. Degradation of the carbon skeleton.
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22-18
Degradaton of Amino Acids, cont.Removal of the a-amino group: Transamination
Requires pyridoxal phosphate, a coenzyme derived from vitamin B6.
-ketoglutarateaspartate
CH2
CH2
C
COO-
COO-
O
CH2
CH
COO-
NH3
+
COO-+
CH2
CH2
CH
COO-
COO-
NH3
+
CH2
C
COO-
OCOO
-+
oxaloacetate glutamate
transaminase
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22-19
Degradaton of Amino Acids, cont.Oxidative Deamination
glutamate
CH2
CH2
CH
COO-
COO-
NH3
+
+ NAD+
+ H2O CH2
CH2
C
COO-
COO-
O + NH4+
+ NADH+ H+
![Page 20: 22-1 Principles and Applications of Inorganic, Organic, and Biological Chemistry Denniston, Topping, and Caret 4th ed Chapter 22 Copyright © The McGraw-Hill.](https://reader036.fdocuments.us/reader036/viewer/2022062315/5697bf931a28abf838c8fc13/html5/thumbnails/20.jpg)
22-20
Fate of Carbon Skeleton
Insert Fig 22.8 4th and caption
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22-21
22.8 the Urea CycleOxidative deamination produces large
amounts of ammonium ion which is toxic.
The pathway to eliminate ammonium ion is the Urea Cycle.
Urea is excreted in the urine.
Failure of enzymes in the urea cycle leads to hyperammonemia. In severe cases this leads to early death from toxic ammonium ion buildup. It can also lead to retardation, convulsions, and vomiting.
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22-22
NH2
C OOPO3
2-
CO2 + NH4+ H2O
2 ATP2(ADP + Pi)
carbamoyl phosphate
NH2 CH2 CHNH3
+
COO-
3
NH2 CO
NH CH2 CHNH3
+
COO-
3
ornithine
citruline
In themitochondria
To cytosol
Urea Cycle-1
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22-23
NH2 CO
NH CH2 CHNH3
+
COO-
3
-OOC CH
N
CH2COO-
NH2 C NH CH2 CHNH3
+
COO-
3
arginosuccinate
aspartate
Urea Cycle-2
incytosol
-OOC CH
NH3
+CH2COO
-
ATP
AMP + PPi
![Page 24: 22-1 Principles and Applications of Inorganic, Organic, and Biological Chemistry Denniston, Topping, and Caret 4th ed Chapter 22 Copyright © The McGraw-Hill.](https://reader036.fdocuments.us/reader036/viewer/2022062315/5697bf931a28abf838c8fc13/html5/thumbnails/24.jpg)
22-24
-OOC CH
N
CH2COO-
NH2 C NH CH2 CHNH3
+
COO-
3
-OOC C
HCH
COO-
NH2
+
NH2 C NH CH2 CHNH3
+
COO-
3
fumarate
arginine
NH2
NH2 C O
H2O
ornithine
urea!
Urea Cycle-3
In cytosol
![Page 25: 22-1 Principles and Applications of Inorganic, Organic, and Biological Chemistry Denniston, Topping, and Caret 4th ed Chapter 22 Copyright © The McGraw-Hill.](https://reader036.fdocuments.us/reader036/viewer/2022062315/5697bf931a28abf838c8fc13/html5/thumbnails/25.jpg)
22-25
Starthere
NH2C OOPO3
2-
CO2 + NH4
+
Urea Cycle
H2O
2 ATP 2(ADP+Pi)
carbamoylphosphate
NH2 CO
NH CH2 CHNH3
+
COO-
3
ornithine
citrulline
arginosuccinate-OOC CH
NH3
+CH2COO
-
aspartate
ATP
AMP + PPi
-OOC CH
NCH2COO
-
NH2 C NH CH2 CHNH3
+
COO-
3
-OOC CH CH COO
-fumarate
NH2
+
NH2 C NH CH2 CHNH3
+
COO-
3arginine
NH2 CO
NH2ureaNH2 CH2 CH
NH3
+
COO-
3+
H2O
![Page 26: 22-1 Principles and Applications of Inorganic, Organic, and Biological Chemistry Denniston, Topping, and Caret 4th ed Chapter 22 Copyright © The McGraw-Hill.](https://reader036.fdocuments.us/reader036/viewer/2022062315/5697bf931a28abf838c8fc13/html5/thumbnails/26.jpg)
22-26
22.9 Overview of Anabolism
Insert Fig 22.13
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22-27
The End
Aerobic Respiration
And
Energy Production