2021

JANUARYLILIAN SCHIMMEL, EMMA GORDON

INSTITUTE FOR MOLECULAR BIOSCIENCE, THE UNIVERSITY OF QUEENSLAND, BRISBANE, AUSTRALIA

A murine metatarsal as an ex vivo model for sprouting angiogenesis. The fetal mouse bone was obtained from an E16.5 LifeAct-GFP mouse embryo and cultured for 14 days in an ibidi µ-Plate 24 Well, leading to the outgrowth of blood vessels. The metatarsal shows the actin cytoskeleton in green, and was stained for CD31 (PECAM-1) in red as an endothelial marker to highlight vessel outgrowth from the bone.

FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN

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MIRJAM BACHLER, MARTIN HERMANN, DIETMAR FRIES

DEPARTMENT OF ANESTHESIOLOGY AND CRITICAL CARE MEDICINE, MEDICAL UNIVERSITY INNSBRUCK, AUSTRIA

Biopsychronology of a COVID-19 patient blood sample in the ibidi µ-Slide 8 Well after induction of coagulation. The right side shows the area of the blood clot with the fibrin network (blue) and the erythrocytes (red). Note the abundant granulocytes (their nuclei labeled in green) fighting against COVID-19 and other enemies of our health. Image acquisition was performed on a spinning disc confocal microscope.

FEBRUARY

MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN

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MARCHASHLEY CHIN

MONTREAL CLINICAL RESEARCH INSTITUTE (IRCM), MCGILL UNIVERSITY, CANADA

The new chain of lives. Immunofluorescence of Drosophila ovarioles showing egg chambers at various stages in time, from a young stem cell on the left to more matured egg chambers on the right. Notice how the change in shape is a dynamic process. Cy3 (green) reveals the expression of a polarity protein, nuclei are stained with DAPI (red). The image was acquired using a Zeiss LSM 700 confocal microscope with a 20x objective.

MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

MAXIMILIAN FUSENIG, NICHOLAS DENNISON

LEIBNIZ INSTITUTE OF POLYMER RESEARCH, MAX BERGMANN CENTER OF BIOMATERIALS, TECHNICAL UNIVERSITY OF DRESDEN, GERMANY

Complex network formation of human bone marrow-derived mesenchymal stromal cells (MSCs) and human umbilical vein endothelial cells (HUVECs), embedded in a degradable (poly)ethyleneglycol-heparin hydrogel. HUVECs are distinguished via CD31 expression (green), F-actin is labelled with phalloidin (red), and nuclei are stained with DRAQ5™ (blue). Image was acquired with a 10x objective on an Andor Dragonfly confocal microscope.

APRIL

THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI

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TIM SELF

SCHOOL OF LIFE SCIENCES IMAGING (SLIM), MEDICAL SCHOOL, UNIVERSITY OF NOTTINGHAM, UNITED KINGDOM

Lily pollen was placed onto an ibidi Glass Bottom Dish 35 mm and the natural autofluorescence from the pollen was captured in the DAPI / FITC / Rhodamine channels. The image was captured on a Zeiss Observer microscope using a 40x oil objective.

MAY

SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON

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TOMASZ J. NAWARA

DEPARTMENT OF CELL, DEVELOPMENTAL AND INTEGRATIVE BIOLOGY, UNIVERSITY OF ALABAMA AT BIRMINGHAM, AL, USA

Live cell imaging of a COS-7 cell using Total Internal Reflection Fluorescence (TIRF) microscopy. The cell expresses a clathrin light chain A, tagged with GFP. Time is color-coded: early clathrin accumulations at the plasma membrane are colored in blue, and the events at the end of the imaging sequence are colored red. The image was acquired using a Nikon Eclipse Ti2 microscope with an Apo TIRF 60x objective.

JUNE

TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED

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JULYILARIA TONAZZINI

ISTITUTO NANOSCIENZE @NEST, CONSIGLIO NAZIONALE DELLE RICERCHE, PISA, ITALY

Confocal microscopy image of primary murine cortical neurons (Div3) cultured on 10 µm-width lines of Poly-L-lysine (PLL) conjugated to fluorescein (green). The line pattern has been developed by soft lithography on glass slides. Neurons were immunostained for Map-2 (yellow), actin fibers were stained with phalloidin (red), nuclei with DAPI (blue). The image was acquired using a Zeiss LSM700 confocal microscope with a 20x objective.

THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

GILLIAN LUDWIG, DANIEL NETTERSHEIM

TRANSLATIONAL UROONCOLOGY, DEPARTMENT OF UROLOGY, UNIVERSITY HOSPITAL DÜSSELDORF, GERMANY

This image shows the three-dimensional growth of germ cell tumor cells (green, GFP) and immune cells (lymphocytes, red, mCherry) using the hanging drop technique. Cell nuclei appear in blue (DAPI). In 3D culture, a close interaction between the diverse cell types develops. The drops were transferred into an ibidi µ-Plate 96 Well. The image was acquired using a Zeiss LSM 710 confocal microscope with a 10x objective.

AUGUST

SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE

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LOUISE GRIVEAU, EMILIE CHRISTIN

INSTITUT NEUROMYOGÈNE, INSERM, CNRS, UNIVERSITÉ CLAUDE BERNARD LYON 1, FRANCE

Cellular van Gogh, inspired by the famous “The Starry Night”. Immunofluorescence of immortalized human myoblasts cultured in an ibidi µ-Slide 2 Well Ph+ coated with Matrigel®. The image shows myoblast fusion with myotubes expressing myosin heavy chain protein (yellow). Phalloidin (blue) was used for actin staining, DAPI (cyan) for nuclear staining. The cells were imaged with a Zeiss Axio Imager Z2 confocal microscope using a 20x objective.

SEPTEMBER

WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU

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OLGA TAPIA1, ALMUDENA MEDINA2

1 HEALTH RESEARCH INSTITUTE VALDECILLA (IDIVAL) AND 2 DEPT. ANATOMY & CELL BIOLOGY, UNIVERSITY OF CANTABRIA, SANTANDER, SPAIN

MCF7 breast cancer cells during the different stages of mitosis. Cells were grown on an ibidi Glass Bottom Dish 35 mm and stained with phalloidin (blue) to visualize F-actin, α-Tubulin for the mitotic spindle (green), and DAPI (red) for DNA. Cells were visualized on a NIKON A1R scanning laser confocal microscope using a 60x objective.

OCTOBER

FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN

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UDESH DHAWAN

INSTITUTE OF CHEMISTRY, ACADEMIA SINICA, TAIPEI, TAIWAN

Immunocytochemistry image of MDA-MB-231 breast cancer cells, showing the formation of tumor-initiating cell clusters that are enriched in cancer stem cell bio-markers. F-actin, which highlights the cytoskeleton, is visualized using phalloidin (green), nuclei are stained with DAPI (blue).

NOVEMBER

MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE

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DECEMBERLOUISIANE PERRIN

BIOENGINEERING DEPARTMENT, COLLEGE OF ENGINEERING, TEMPLE UNIVERSITY, PHILADELPHIA, PA, USA

Image of an adipocyte spheroid labeled with the lipid dye BODIPY. The color scale indicates the z-depth. This adipocyte spheroid was obtained by differentiation of a human mesenchymal stem cell spheroid. The image was acquired using an Olympus laser scanning confocal microscope (FV1200) with a 20x objective.

WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

ASHLEY CHIN

MONTREAL CLINICAL RESEARCH INSTITUTE (IRCM), MCGILL UNIVERSITY, CANADA

The new chain of lives. Immunofluorescence of Drosophila ovarioles showing egg chambers at various stages in time, from a young stem cell on the left to more matured egg chambers on the right. Notice how the change in shape is a dynamic process. Cy3 (green) reveals the expression of a polarity protein, nuclei are stained with DAPI (red). The image was acquired using a Zeiss LSM 700 confocal microscope with a 20x objective.

MIRJAM BACHLER, MARTIN HERMANN, DIETMAR FRIES

DEPARTMENT OF ANESTHESIOLOGY AND CRITICAL CARE MEDICINE, MEDICAL UNIVERSITY INNSBRUCK, AUSTRIA

Biopsychronology of a COVID-19 patient blood sample in the ibidi µ-Slide 8 Well after induction of coagulation. The right side shows the area of the blood clot with the fibrin network (blue) and the erythrocytes (red). Note the abundant granulocytes (their nuclei labeled in green) fighting against COVID-19 and other enemies of our health. Image acquisition was performed on a spinning disc confocal microscope.

ILARIA TONAZZINI

ISTITUTO NANOSCIENZE @NEST, CONSIGLIO NAZIONALE DELLE RICERCHE, PISA, ITALY

Confocal microscopy image of primary murine cortical neurons (Div3) cultured on 10 µm-width lines of Poly-L-lysine (PLL) conjugated to fluorescein (green). The line pattern has been developed by soft lithography on glass slides. Neurons were immunostained for Map-2 (yellow), actin fibers were stained with phalloidin (red), nuclei with DAPI (blue). The image was acquired using a Zeiss LSM700 confocal microscope with a 20x objective.

Follow Ilaria Tonazzini, @neuroguide2018, and NeuroSens_NEST on Facebook.

OLGA TAPIA 1, ALMUDENA MEDINA 2

1 HEALTH RESEARCH INSTITUTE VALDECILLA (IDIVAL) AND 2 DEPT. ANATOMY & CELL BIOLOGY, UNIVERSITY OF CANTABRIA, SANTANDER, SPAIN

MCF7 breast cancer cells during the different stages of mitosis. Cells were grown on an ibidi Glass Bottom Dish 35 mm and stained with phalloidin (blue) to visualize F-actin, α-Tubulin for the mitotic spindle (green), and DAPI (red) for DNA. Cells were visualized on a NIKON A1R scanning laser confocal microscope using a 60x objective.

UDESH DHAWAN

INSTITUTE OF CHEMISTRY, ACADEMIA SINICA, TAIPEI, TAIWAN

Immunocytochemistry image of MDA-MB-231 breast cancer cells, showing the formation of tumor-initiating cell clusters that are enriched in cancer stem cell biomarkers. F-actin, which highlights the cytoskeleton, is visualized using phalloidin (green), nuclei are stained with DAPI (blue).

Follow @theamateurpostdoc on Instagram.

TIM SELF

SCHOOL OF LIFE SCIENCES IMAGING (SLIM), MEDICAL SCHOOL, UNIVERSITY OF NOTTINGHAM, UNITED KINGDOM

Lily pollen was placed onto an ibidi Glass Bottom Dish 35 mm and the natural autofluorescence from the pollen was captured in the DAPI / FITC /Rhodamine channels. The image was captured on a Zeiss Observer microscope using a 40x oil objective.

Follow @SLIM_nottingham on Twitter.

TOMASZ J. NAWARA

DEPARTMENT OF CELL, DEVELOPMENTAL AND INTEGRATIVE BIOLOGY, UNIVERSITY OF ALABAMA AT BIRMINGHAM, AL, USA

Live cell imaging of a COS-7 cell using Total Internal Reflection Fluorescence (TIRF) micros- copy. The cell expresses a clathrin light chain A, tagged with GFP. Time is color-coded: early clathrin accumulations at the plasma membrane are colored in blue, and the events at the end of the imaging sequence are colored red. The image was acquired using a Nikon Eclipse Ti2 microscope with an Apo TIRF 60x objective.

Follow @nawara_tomasz on Twitter and @mattheyseslab on Instagram.

LOUISIANE PERRIN

BIOENGINEERING DEPARTMENT, COLLEGE OF ENGINEERING, TEMPLE UNIVERSITY, PHILADELPHIA, PA, USA

Image of an adipocyte spheroid labeled with the lipid dye BODIPY. The color scale indicates the z-depth. This adipocyte spheroid was obtained by differentiation of a human mesenchymal stem cell spheroid. The image was acquired using an Olympus laser scanning confocal microscope (FV1200) with a 20x objective.

Follow @LouisianePerrin on Twitter.

LOUISE GRIVEAU, EMILIE CHRISTIN

INSTITUT NEUROMYOGÈNE, INSERM, CNRS, UNIVERSITÉ CLAUDE BERNARD LYON 1, FRANCE

Cellular van Gogh, inspired by the famous “The Starry Night”. Immunofluorescence of immortalized human myoblasts cultured in an ibidi µ-Slide 2 Well Ph+ coated with Matrigel®. The image shows myoblast fusion with myo- tubes expressing myosin heavy chain protein (yellow). Phalloidin (blue) was used for actin staining, DAPI (cyan) for nuclear staining. The cells were imaged with a Zeiss Axio Imager Z2 confocal microscope using a 20x objective.

Follow @aminfakhree on Instagram.

MAXIMILIAN FUSENIG, NICHOLAS DENNISON

LEIBNIZ INSTITUTE OF POLYMER RESEARCH, MAX BERGMANN CENTER OF BIOMATERIALS, TECHNICAL UNIVERSITY OF DRESDEN, GERMANY

Complex network formation of human bone marrow-derived mesenchymal stromal cells (MSCs) and human umbilical vein endothelial cells (HUVECs), embedded in a degradable (poly)ethyleneglycol-heparin hydrogel. HUVECs are distinguished via CD31 expression (green), F-actin is labelled with phalloidin (red), and nuclei are stained with DRAQ5™ (blue). Image was acquired with a 10x objective on an Andor Dragonfly confocal microscope.

Follow Maximilian Fusenig and Nicholas Dennison on LinkedIn.

GILLIAN LUDWIG, DANIEL NETTERSHEIM

TRANSLATIONAL UROONCOLOGY, DEPARTMENT OF UROLOGY, UNIVERSITY HOSPITAL DÜSSELDORF, GERMANY

This image shows the three-dimensional growth of germ cell tumor cells (green, GFP) and immune cells (lymphocytes, red, mCherry) using the hanging drop technique. Cell nuclei appear in blue (DAPI). In 3D culture, a close interaction between the diverse cell types develops. The drops were transferred into an ibidi µ-Plate 96 Well. The image was acquired using a Zeiss LSM 710 confocal microscope with a 10x objective.

JANUARY

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FEBRUARY MARCH APRIL MAY JUNE

JULY AUGUST SEPTEMBER OCTOBER NOVEMBER DECEMBER

LILIAN SCHIMMEL, EMMA GORDON

INSTITUTE FOR MOLECULAR BIOSCIENCE, THE UNIVERSITY OF QUEENSLAND, BRISBANE, AUSTRALIA

A murine metatarsal as an ex vivo model for sprouting angiogenesis. The fetal mouse bone was obtained from an E16.5 LifeAct-GFP mouse embryo and cultured for 14 days in an ibidi µ-Plate 24 Well, leading to the outgrowth of blood vessels. The metatarsal shows the actin cytoskeleton in green, and was stained for CD31 (PECAM-1) in red as an endothelial marker to highlight vessel outgrowth from the bone.

Follow @VesselDynamics on Twitter.