2020 – VOLUME II NEWSLETTER / BULLETIN - Volume II - Newsletter.pdfNEWSLETTER / BULLETIN Dear...
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Canadian Embryo Transfer Association / Association Canadienne de Transfer d’Embryons 2020 – VOLUME II NEWSLETTER / BULLETIN Dear CETA/ACTE Members, As I am writing this, the summer is in full swing. In Ontario, Québec and the Maritimes it has been a very hot and dry summer so far as opposed to Western Canada who has experienced a cooler and wet summer. As practitioners, we are always very busy; summer is a good time to spend some time with the family and to take some time for yourselves such as going on hikes, cycling, rollerblading, swimming, etc. I promised myself I would not talk about the Covid-19 pandemic! It is very hard not to do so as it has made us cancel the AETA & CETA/ACTE joint convention in Madison. When Covid hit us in March, we all thought that it would be over by the fall but it looks like it will be here for a while. The good news in all of that, if there is one, is that AETA/CETA will get the deposit back from the venue and there will be no penalty for cancelling. AETA has been busy in trying to figure out how to deliver a virtual conference. More details will be coming out very soon as you will have to register for the virtual joint convention. It will be a totally new concept. I strongly believe that we can deliver good quality CE with such a format. As you are aware, we had started the process of finding a replacement for Karen. I am happy to report that we have hired the new secretary-manager for CETA/ACTE. Carole Ness of Howick, Québec will be replacing Karen in the new year. She brings everything we were looking for; she is a detailed focus manager with loads of experience in multi-tasking, office management, book keeping and computer knowledge. As the CETA/ACTE office will be moving to Québec in the new year, the Articles for CETA/ACTE filed with the Canada Not-for-Profit Corporations Act will need to be changed, as well as a couple of By-law changes. These will be presented at the AGM which will be held virtually this year on October 7. This is my last president’s message. After two terms on the CETA/ACTE board, I will give someone else the chance to lead this organization in the next decade. Stay healthy! Dr Luc Besner In this Issue… President’s message …………………. Notice of AGM …………………………. Membership Notices ………………… In Memoriam: Dr. Vlad Pawlyshyn …………………… 2020 Joint Convention ……………. Upcoming CE Opportunities …… 2019 ET Summary in Canada …… Interesting Web Sites …………… Companies with ET software … Article of Interest …………………. 1 2 4 5 7 7 9 10 10 12-13 Mark Your Calendars! 2020 AETA & CETA/ACTE JOINT VIRTUAL CONVENTION October 6-7, 2020 CETA/ACTE OFFICE Canadian Embryo Transfer Association Association Canadienne de Transfert d’Embryons P.O. Box 39 Kemptville, Ontario, Canada K0G 1J0 Tel: 613-340-1536 Fax: 613-652-1421 Email: [email protected] Web site: www.ceta.ca Note: For courier address, please contact the office.
Transcript of 2020 – VOLUME II NEWSLETTER / BULLETIN - Volume II - Newsletter.pdfNEWSLETTER / BULLETIN Dear...
Canadian Embryo Transfer Association / Association Canadienne de Transfer d’Embryons
2020 – VOLUME II
NEWSLETTER / BULLETIN
Dear CETA/ACTE Members,
As I am writing this, the summer is in full swing. In Ontario, Québec and the Maritimes it has been a very hot and dry summer so far as opposed to Western Canada who has experienced a cooler and wet summer. As practitioners, we are always very busy; summer is a good time to spend some time with the family and to take some time for yourselves such as going on hikes, cycling, rollerblading, swimming, etc.
I promised myself I would not talk about the Covid-19 pandemic! It is very hard not to do so as it has made us cancel the AETA & CETA/ACTE joint convention in Madison. When Covid hit us in March, we all thought that it would be over by the fall but it looks like it will be here for a while. The good news in all of that, if there is one, is that AETA/CETA will get the deposit back from the venue and there will be no penalty for cancelling.
AETA has been busy in trying to figure out how to deliver a virtual conference. More details will be coming out very soon as you will have to register for the virtual joint convention. It will be a totally new concept. I strongly believe that we can deliver good quality CE with such a format.
As you are aware, we had started the process of finding a replacement for Karen. I am happy to report that we have hired the new secretary-manager for CETA/ACTE. Carole Ness of Howick, Québec will be replacing Karen in the new year. She brings everything we were looking for; she is a detailed focus manager with loads of experience in multi-tasking, office management, book keeping and computer knowledge.
As the CETA/ACTE office will be moving to Québec in the new year, the Articles for CETA/ACTE filed with the Canada Not-for-Profit Corporations Act will need to be changed, as well as a couple of By-law changes. These will be presented at the AGM which will be held virtually this year on October 7.
This is my last president’s message. After two terms on the CETA/ACTE board, I will give someone else the chance to lead this organization in the next decade.
Stay healthy!
Dr Luc Besner
In this Issue…
President’s message ………………….
Notice of AGM ………………………….
Membership Notices …………………
In Memoriam: Dr. Vlad Pawlyshyn ……………………
2020 Joint Convention …………….
Upcoming CE Opportunities ……
2019 ET Summary in Canada ……
Interesting Web Sites ……………
Companies with ET software …
Article of Interest ………………….
1
2
4
5
7
7
9
10
10
12-13
Mark Your Calendars! 2020 AETA & CETA/ACTE
JOINT VIRTUAL CONVENTION
October 6-7, 2020
CETA/ACTE OFFICE Canadian Embryo Transfer Association
Association Canadienne de Transfert d’Embryons P.O. Box 39
Kemptville, Ontario, Canada K0G 1J0 Tel: 613-340-1536 Fax: 613-652-1421
Email: [email protected] Web site: www.ceta.ca
Note: For courier address, please contact the office.
CETA/ACTE Newsletter / Bulletin ~ 2 ~ 2020 – Volume II
CETA/ACTE BOARD OF DIRECTORS
EXECUTIVE: Dr. Luc Besner (PRESIDENT) Embrun Veterinary Clinic 651 Notre Dame, Embrun, ON K0A 1W1 Tel: 613-443-5489 Fax: 613-987-2669 Email: [email protected]
Dr. Lisa McCrea Hemphill (VICE PRESIDENT) Agwest Veterinary Group Ltd. 1625 Angus Campbell Rd. Abbotsford, BC V3G 2G4 Tel: 604-826-5089 Fax: 604-826-5030 Email: [email protected]
Dr. Blair Simonson Dr. Simonson Veterinary Prof. Corp. Box 57, R.R. #6, Site 601 Saskatoon, SK S7K 3J9 Tel: 306-291-4680 Email: [email protected]
Dr. Jonathan Lehouiller Medi-Vet Inc. 167 rue Notre-Dame Notre-Dame-du-Bon-Conseil, QC J0C 1A0 Tel: 819-336-5050 Fax: 819-336-5051 Email: [email protected]
DIRECTORS: Dr. Andres Arteaga Bova-Tech Ltd. Box 80142 Airdrie, AB T4B 2V8 Tel: 403-332-1567 Fax: 403-980-3498 Email: [email protected]
Dr. Yves Martin Clinique Vétérinaire de l’Erable inc. 500 Route 165 Sud Plessisville, QC G6L 2Y2 Tel: 819-362-2728 Fax: 819-362-3840 Email: [email protected]
Dr. Jean-François Riverin Service Vétérinaire Ambulatoire St-Vallier 400 Montée de la Station St-Vallier, QC G0R 4J0 Tel: 418-884-3594 Fax: 418-884-3598 Email: [email protected]
Dr. Frank Robblee Kensington Veterinary Clinic Ltd. P.O. Box 10, Kensington, PE C0B 1M0 Tel: 902-432-0596 Email: [email protected]
BOARD ADVISORS: Dr. Anne Holmes (CFIA Representative) Canadian Food Inspection Agency 59 Camelot Drive, Ottawa, ON K1A 0Y9 Tel: 613-773-7121 Email: [email protected]
Michael Hall (CLGA Representative) Canadian Livestock Genetics Association P.O. Box 878, Kemptville, ON K0G 1J0 Tel: 613-258-1585 Email: [email protected]
NOTICE OF 2020 AGM CETA/ACTE Annual General Meeting
HELD VIRTUALLY Wednesday, October 7, 2020 at 9:00 AM (central daylight time)
Your Board of Directors will report on activities of the Association and its committees.
Members will vote on the “Proposed By-law Amendments” and amendment to “Articles” filed with the Canada Not-For-Profit Corporations Act (NFP Act).
The nomination committee will present nominations at the Annual Meeting for election of three Directors for a three-year term. Nominations will also be received from the floor at the meeting.
(Term of office for three Directors namely Luc Besner (ON), Jonathan Lehouiller (QC) and Frank Robblee (PE) will end when their successor is elected.)
We strongly encourage your participation. Come and voice your opinions, concerns and your new ideas.
AVIS DE CONVOCATION À L’ASSEMBLÉE GÉNÉRALE ANNUELLE PAR VIDÉOCONFÉRENCE
Le mercredi 7 octobre 2020 à 9:00 h (heure avancée centrale) Votre conseil d’administration fera rapport des activités de l’Association et de
ses comités.
Les membres devront votés pour adopter de nouveaux statuts et règlements conformément à la loi canadienne sur les organizations à but non lucratif.
Lors de l’assemblée annuelle, le comité de nomination présentera les nominations pour l’élection des trois directeurs qui seront élus pour un mandat de trois ans. Les nominations seront également acceptées lors de l’assemblée annuelle.
(Les mandats des trois directeurs, Luc Besner (ON), Jonathan Lehouiller (QC) et Frank Robblee (PE) se terminera dès l’élection de leurs successeurs.)
Nous vous encourageons fortement à participer à cette assemblée. Venez exprimer votre opinion, vos préoccupations et vos nouvelles idées.
2019-2020 COMMITTEES & CHAIRMEN
Executive: Dr. Luc Besner Health Regulations & Industry Liaison: Dr. Frank Robblee
Certification: Dr. Lisa McCrea Hemphill Legal & Ethics: Dr. Blair Simonson
Research & Education: Dr. Jonathan Lehouiller Convention: Dr. Andres Arteaga, Chair
& Dr. Yves Martin Members' Services: Dr. Jean-François Riverin
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CETA/ACTE Newsletter / Bulletin ~ 4 ~ 2020 – Volume II
MEMBERSHIP NOTICES
From the CETA/ACTE Office:
2019-2020 Annual Report: The Annual Report will be emailed to members. The report will include draft financial statements, committee reports, proposed amendments to the CETA/ACTE By-laws and proposed amendments to ‘Articles’. Please print a copy for the AGM held virtually on Wednesday, Oct 7, 2020.
CETA/ACTE Certified Practitioners: A CE/Embryo Summary was emailed to each certified member the beginning of July, please keep this information on file for easy reference regarding:
CE credits: the number you currently have on file and the number of credits needed for re-certification.
Number of embryos processed: the number you currently have on file and the number still required for re-certification.
Also included on your summary is the date that you will apply for re-certification and the date the requirements must be completed for re-certification.
APPLYING FOR CETA/ACTE CERTIFICATION:
To apply for CETA/ACTE Certification an application and the required information must be submitted to the CETA/ACTE office by May 1 (late applications will be accepted up to June 30th and levied a late penalty fee). Once your application and all information are received it will be reviewed by the Certification Committee to determine if you have met the requirements and are eligible to write the CETA/ACTE Certification Exam. Please note, once successful with the written exam, a mandatory site visit (practical exam) is required. If you are interested in applying for certification, please note the following: Please contact the CETA/ACTE office and request an
application to apply for certification, please indicate the species and technique(s).
The certification exam is only offered at the time of the annual convention.
NOTE: If you applied for re-certification this year, all CE and Embryo numbers included on your summary will be used for re-certification and cannot be carried over to your new 3-year certification term.
If you did not receive your CE/Embryo summary, please contact the CETA/ACTE office immediately.
CETA/ACTE Web site: The ‘ET Activity in Canada for 2019’ has been posted on the web site, on the ‘ET Activity in Canada’ page.
A minimum of 10 hours of continuing education related to embryo transfer within the past 2 years, is required when applying for certification.
Dates and location of future CETA/ACTE Certification Exams include:
October 2021, Vancouver, British Columbia, Canada October 2022, Louisville, Kentucky, USA October 2023, Orlando, Florida, USA October 2024, Toronto, Ontario, Canada
CETA/ACTE CERTIFICATION The CETA/ACTE Certification program is often used as a model by
other embryo transfer associations in the development of their certification programs.
CETA/ACTE Certification is a required and first step in the Canadian Food Inspection Agency (CFIA) Embryo Export Approval Program (EEAP) in order to export embryos from Canada.
CETA/ACTE Certification is also a measure of excellence and is used by practitioners to promote their expertise in the field of embryo transfer.
-------------------------------- Are you CETA/ACTE Certified?
Do you use and display the CETA/ACTE Certified Logo?
For detailed information, including all of the minimum requirements to apply for certification, please refer to the CETA/ACTE Certification Standards and/or contact the CETA/ACTE office.
CETA/ACTE accepts payment by cheque and ‘INTERAC e-Transfer’. To pay by Interac e-Transfer, please contact your local bank for instructions and fees. Email address to send money via INTERAC e-Transfer: [email protected]
Available at the CETA/ACTE office:
Tank seals: Sold in quantities of 50. Cost: $0.50/tank seal + GST/HST + mailing cost
Embryo Transfer Forms in an electronic (fillable) format (English/French): Form ‘ABC’ (Embryo Recovery-Transfer-Freeze) & ‘D’ (Application for International Embryo Movement).
The certified logo is available at the CETA/ACTE office in English and French in the following formats: jpg, gif & eps
REMINDER: If your email address or contact information changes throughout
the year please advise the CETA/ACTE office. NOTE: If you do not wish to receive correspondence via email,
please contact the CETA/ACTE office.
CETA/ACTE Newsletter / Bulletin ~ 5 ~ 2020 – Volume II
Membership Notices continued…
In Memoriam: Dr. Vlad Pawlyshyn Received from Dr. Reuben Mapletoft:
It is with sadness that we inform membership of the death of Dr. Vlad Pawlyshyn. Vlad passed away peacefully, at home, in Martensville, SK on June 9, 2020.
After graduation from the University of Saskatchewan in Veterinary Medicine (WCVM) in 1975, Dr. Pawlyshyn went to private practice in Langenburg, SK, and then returned to the WCVM in 1980, completing a Master of Veterinary Science under the supervision of Dr. Bill Cates. Vlad was an integral member of the Reproduction Research Trust Unit during the pioneering days of on farm bovine embryo transfer conducted by the WCVM, travelling throughout Saskatchewan, Manitoba and Alberta to provide embryo transfer services for cattle breeders. In 1985, Vlad established a reproduction practice, Trans Tech Genetics, serving clients across Western Canada. Throughout his career, he maintained a close relationship with the WCVM Theriogenology group, providing embryo transfer services for research projects, teaching practitioners in the WCVM Embryo Transfer workshops and training countless graduate students and clinical residents in Special Topics courses in embryo transfer. Vlad was one of the first certified embryo transfer practitioners in Canada, certified by the Canadian Embryo Transfer Association in 1993. He travelled to Argentina to transfer embryos on more than one occasion. Dr. Pawlyshyn was a past board member and president of the Canadian Embryo Transfer Association. He was also a regular attendee at IETS meetings, regardless of where they were held. Dr. Pawlyshyn had many friends throughout the embryo transfer industry and in the wider community of veterinarians, and he will be dearly missed.
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CETA/ACTE Newsletter / Bulletin ~ 7 ~ 2020 – Volume II
AETA & CETA/ACTE Joint Virtual Convention
October 6 - 7, 2020
Registration is open for the 2020 AETA & CETA/ACTE Joint Virtual Convention. The convention will take place virtually from October 6-7 and will feature two types of sessions: keynote addresses and prerecorded sessions. Keynote addresses will be live sessions via Zoom, where participants will see the content and interact with the speakers in real time. Once the keynote addresses have concluded, a link to the content will be posted. Prerecorded sessions will be made available via a link on the AETA convention page at 12:01 am (CDT) on Tuesday, October 6. During the times on the schedule, speakers will be available to chat via Zoom about their presentations and answer any questions you may have. All sessions will have a quiz that participants must participate in and pass with a score of at least 70% to receive continuing education (CE) credits. Please note that speakers will only be available for discussion during their scheduled time. Participants who register before October 6th can view sessions and take the accompanying RACE quizzes until December 31st. You must register before October 6th to get access to the meeting content. Please visit the CETA/ACTE web site for more information on the joint convention at: http://www.ceta.ca/convention.html
FUTURE CETA/ACTE CONVENTIONS
2021 CETA/ACTE & AETA Joint Convention October 14-16, 2021
Westin Bayshore Vancouver Vancouver, British Columbia, Canada
2022 AETA & CETA/ACTE Joint Convention October 27-29, 2022
Louisville Marriott Downtown Louisville, Kentucky, USA
2023 AETA & CETA/ACTE Joint Convention October 26-28, 2023
Rosen Shingle Creek Orlando Orlando, Florida, USA
2024 CETA/ACTE & AETA Joint Convention October 17-19, 2024
Westin Harbour Castle hotel Toronto, Ontario, Canada
UPCOMING CE OPPORTUNITIES & MEETINGS OF INTEREST
August 13-15, 2020 34rd Brazilian Embryo Technology Society (SBTE) Annual Meeting Virtual format (on line) For information, please visit: http://www.sbte.org.br
September 10-11, 2020 36th Scientific Meeting of AETE - “Virtual AETE-Lite” Association of Embryo Technology in Europe (AETE) For information, please visit: https://www.aete.eu/
September 24-26, 2020 AABP Conference American Association of Bovine Practitioners (AABP) Louisville, Kentucky, USA For information, please visit: http://www.aabp.org/
September 29 - October 3, 2020 World Dairy Expo Madison, Wisconsin, USA For information, please visit: https://worlddairyexpo.com/
October 6-7, 2020 2020 AETA & CETA/ACTE Joint Virtual Convention For information, please visit: https://ceta.ca/convention.html
January 16-19, 2021 47th Annual Conference of the IETS VIRTUAL CONFERENCE For information, please visit: https://www.iets.org/2021/
June 27 – July 1, 2021 ICAR 2020 19TH International Congress on Animal Reproduction (ICAR) Bologna, Italy For more information, please visit: http://www.icar2020.org
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CETA/ACTE Newsletter / Bulletin ~ 9 ~ 2020 – Volume II
SUMMARY OF EMBRYO TRANSFER ACTIVITY IN CANADA FOR 2019 IN-VIVO DATA
DONORS ● EMBRYOS COLLECTED ● EMBRYOS FROZEN BOVINE CAPRINE CERVINE EQUINE OVINE PORCINE Total number of donors 7,685 12 0 13 88 25 Total number of transferable embryos collected 48,761 40 0 4 402 363 Total number of embryos frozen 35,301 22 0 0 370 0 EMBRYOS TRANSFERRED BOVINE CAPRINE CERVINE EQUINE OVINE PORCINE Total number of fresh embryos transferred 13,148 18 0 4 32 0 Total number of frozen embryos transferred 21,888 0 111 0 37 0 EXPORT ● IMPORT BOVINE CAPRINE CERVINE EQUINE OVINE PORCINE Total number of embryos exported 7,168 6 0 0 77 363 Total number of embryos imported 191 0 111 0 0 0
IN-VITRO (IVF) DATA
For more details, please refer to the
« Summary of Embryo Transfer Activity in Canada for 2019 »
posted on the CETA/ACTE web site at: http://www.ceta.ca/et-activity.html
SESSIONS ● EMBRYOS COLLECTED ● EMBRYOS FROZEN BOVINE Total number of OPU/IVF sessions 4,675 Total number of transferable IVP embryos produced 24,040 Total number of IVP embryos frozen 13,575 EMBRYOS TRANSFERRED BOVINE Total number of fresh IVP embryos transferred 4,197 Total number of frozen IVP embryos transferred 4,643 EXPORT ● IMPORT BOVINE Total number of IVP embryos exported 1,305
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CETA/ACTE Newsletter / Bulletin ~ 10 ~ 2020 – Volume II
Interesting Web Sites
Agriculture and Agri-Food Canada: ‘Red Meat Market Information’: www.agr.gc.ca/redmeat
Canadian Animal Health Coalition: www.animalhealth.ca
Canadian Animal Health Surveillance Network (CAHSN): https://www.inspection.gc.ca/about-the-cfia/science-
and-research/our-laboratories/cahsn/eng/1524146875354/1524146966702
Canadian Beef Breeds Council: www.canadianbeefbreeds.com
Canadian Dairy Information Centre (CDIC): www.dairyinfo.gc.ca
Canadian Dairy Network: www.cdn.ca
Canadian Food Inspection Agency (CFIA): www.inspection.gc.ca
National Biosecurity Standards: www.inspection.gc.ca/biosecurity
Canadian Livestock Genetics Association (CLGA): www.clivegen.org
The Cattle Network: www.cattlenetwork.net
Engormix: http://en.engormix.com
European Association of Animal Science: www.eaap.org
International Committee for Animal Recording: www.icar.org
International Embryo Technology Society (IETS): www.iets.org
International Veterinary Information Service (IVIS): www.ivis.org
National Farm Animal Care Council: www.nfacc.ca
National Farmed Animal Health and Welfare Council: http://www.ahwcouncil.ca
Scrapie Canada: www.scrapiecanada.ca This site is dedicated to providing information to sheep & goat
producers across Canada about scrapie and associated programs.
World Organisation for Animal Health (OIE): www.oie.int
Have you found an interesting web site to visit? Please send the web address to the CETA/ACTE office
to be included in future issues.
COMPANIES WITH EMBRYO TRANSFER COMPUTER PROGRAMS
AGRIVET Dr. Louis Picard
Email: [email protected] Web site: www.agrivet.net
GENTEC LTD. P.O. Box 579
Kensington, PE C0B 1M0 Tel: 902-836-4119
Email: [email protected]
MINITUBE CANADA Program: Herd Vision
(Note: an option is available to upgrade the program ‘Phoenix’ to ‘Herd Vision’)
Contact: Sales Tel: 1-800-500-7978
Email: [email protected]
Web site: www.minitube.com
MULLER SYSTEMS CORPORATION Program: ETS
926 Juliana Drive, Woodstock, ON N4V 1B9
Tel: 519-421-1800 Email: [email protected]
Web site: www.mullersys.com
S.Q.L. a/s Ovila Landry
C.P. 8571 Succ. Ste-Foy Quebec, QC, G1V 4N5
Tel: 418-871-9420 Email: [email protected]
ROGER W. THOMPSON DVM (Program is a Microsoft Access
database program use for all bovine and small ruminant embryo transfer
record keeping.) Tel: 614-570-7098
Email: [email protected]
DO YOU OFFER EMBRYO TRANSFER TRAINING? CETA/ACTE is often contacted for information regarding embryo transfer
training. If you offer embryo transfer training and would like to be included on the list that CETA/ACTE has on file, please contact the CETA/ACTE office at:
REMINDER: PLEASE PARENTAGE TEST ALL ET DONORS Where to send genetic testing:
GTM Testing for all COLOURED BREEDS: Send Directly to GenServe
GTM Testing for HOLSTEIN: Send to Holstein Canada
GTS Testing for ALL BREEDS: Send to Holstein Canada
LABELLING
If you receive frozen embryos that are not labelled according to IETS standards, please contact the CETA/ACTE office with the following details: a) How is the embryo identified / labelled (cane top, straw, goblet)? b) If the embryo(s) are imported does the labelling on the straw match the
export certificate? c) Please include who sent the embryos.
Our goal is to improve all labelling and help those who struggle to maintain label quality.
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CETA/ACTE Newsletter / Bulletin ~ 12 ~ 2020 – Volume II
Article of Interest
The Article of Interest is a summary of the study « Bovine oviductal and uterine fluid support in vitro embryo development », by M. Hamdi., D. Rizos et al., from the Reproduction, Fertility and Development, November 2017. You can refer to this article for more details.
----------------------------
Bovine oviductal and uterine fluid support in vitro embryo development
Summary by: Dr Jonathan Lehouiller, Chair, CETA/ACTE Research & Education Committee
The study investigated the effect of oviductal fluid (OF) and/or uterine fluid (UF) supplementation on in vitro embryo development and quality. A lot of advancement has been achieved in the last decade in in vitro embryo production (IVP). However, the quality of IVP blastocysts are still considered lower than in vivo produced blastocysts. Their morphology, metabolism and cryotolerance are different. Modification of the in vitro culture environment can improve blastocyst production and quality. Supplement like fetal calf serum (FCS) or bovine serum albumin (BSA) are commonly used in culture media. FCS, despite the fact that it accelerates embryo development, have been associated with a lower blastocyst quality and fetal overgrowth at birth. Lots of studies have been done on different oviductal molecules but there is a limited number of studies regarding OF or UF supplementation during IVC.
After collecting cumulus oocytes-complexes (COCs) from slaughterhouse ovaries from mature heifers, standard in vitro maturation and in vitro fertilization was achieved. Presumptives zygotes were splitted 18 to 20 hours after fertilization in five different groups for IVC: 1- Synthetic oviduct fluid (SOF) supplemented with 5% FCS (SOF+FCS) 2- SOF supplemented with bovine serum albumin (SOF+BSA) 3- SOF with 1,25% OF from day 1 to day 4 (OF group) 4- SOF with 1,25% OF from day 1 to day 4 followed by 1,25% UF from day 4 to day 9 (OF+UF group) and 5- SOF with 1,25% UF from day 4 to day 9 only (UF group). The ability of the blastocyst to tolerate cryopreservation was used as an indicator of quality. Gene expression analysis was also performed on the blastocyst from the different groups, as well as evaluation of the reactive oxygen species (ROX) quantification via fluorescence. Results
On the embryo development side, no difference in the cleavage rate were noted between all groups. Blastocyst production was higher at Day 7 in the SOF+FCS group compared to SOF+BSA and OF groups, but similar to OF+ UF and UF groups. At Day 8 and 9, no differences were observed on the blastocyst rate of all groups.
The table 1 represent the survival rates after vitrification/warming of Day 7 Blastocyst cultured in the different groups.
Blastocyst survival rate (%) at different time after vitrification/warming
4h 24h 48h 72h SOF+ FCS 66.5 59.0 41.1 31.6 SOF+BSA 86.2 74.2 73.3 64.8 OF+UF 87.1 70.2 62.8 62.8 OF 83.2 70.7 61.0 61.0 UF 84.7 69.5 53.3 53.3
CETA/ACTE Newsletter / Bulletin ~ 13 ~ 2020 – Volume II
Article of Interest continued…
The blastocyst survival rate 24h after warming was not different between groups, ranging from 59.0% to 74.2. After 48h, survival rates of blastocysts obtained from culture with OF+UF and OF were similar to those of the SOF+BSA group, and significantly higher than those of the SOF+FCS group.
In relation to the gene expression, expression of the epigenetic control genes DNMT3A and IGF2R (genes important in embryo methylation) were upregulated in the OF compared to the SOF+FCS. Also, oxidative stress regulation genes was downregulated in the UF and OF+UF groups compared to SOF+BSA and OF groups.
The table 2 represent the relative reactive oxygen species (ROS) between groups.
Relative ROS fluorescence intensity was significantly lower in blastocysts from the OF+UF, OF and UF groups compared with blastocysts from the SOF+FCS groups. Also, ROS levels were significantly lower in OF+UF groups compared to SOF+BSA.
Discussion
The main finding of the present study is that a low concentration of OF and/or UP supports embryo development and improves quality. OF seems to provide control in embryo methylation, whereas UF seems to provide antioxidant activity.
Despite the successful improvement of IVP in cattle, there is evidence that post fertilisation culture environment have major impact on blastocyst quality. There is also evidence of a marked contrast in the pattern of gene expression related to lipid metabolism and oxidative stress response when comparing in vitro produced blastocyst to those produced in vivo, with an impact on embryo tolerance to cryopreservation. In this study, culturing embryo with OF seems to support embryo development, and similar effects were observed in other studies. However, this study suggest that alternating OF with UF during the culture period might mimic more accurately the physiological conditions in vivo. The period studied is a time of major developmental events occurring in the embryo, such as first cleavage, embryonic genome activation, morula compaction, blastocyst formation and hatching. As those events appear to be sensitive to culture conditions, there might be a positive effect of adapting the culture condition during the IVC period instead of using a static IVC.
In vitro production and manipulation of embryos increase reactive oxidative species generation. This is related with impaired development of embryos. Conclusion
In conclusion, low concentrations of OF and UF with serum-free culture media supports embryo development, blastocyst quality and cryotolerance. This evidence might lead to the discovery of specific proteins important for embryo development and help improve the success of IVP. ֎
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SOF+FCS SOF+BSA OF+UF OF UF
ROS Fluorescence intensity (a.u.)
ROS Fluorescence intensity (a.u.)
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