Abstractsdownloads.hindawi.com/journals/jamc/1986/532707.pdf · 2019-08-01 · A program is...
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Journal of Automatic Chemistry of Clinical Laboratory Automation, Vol. 8, No. 2 (April-June 1986), pp. 45-48 Abstracts Page 49 Computer acquisition and analy- sis of data in enzymatic- fluorimetric-continuous-flow methods for the measurement of glucose, lactate, pyruvate, ala- nine, glycerol and 3-hydroxy- butyrate in human blood C. S. Hetherington et al. A program is described to acquire and process data using an Apple computer in the determination of human blood glucose, lactate, pyru- vate, alanine, glycerol and 3-hydroxybutyrate. The assay methods are enzymatic and combine continuous-flow systems with flu- orimetry. Particular attention in the program is given to the necessity to correct for the blank fluorescence present in perchloric extracts of blood, although it can also be used for a variety of assays where ’blank’ runs are not required. Using peak height measurements the blood metabolite concentration is deter- mined by a software package consist- ing of four subprograms, which allow flexibility, although a rigid sequence of recurring groups of standards, controls and samples is necessary. In the ’acquisition’ subprogram, data can be acquired from three fluorimet- ers simultaneously. The ’amend’ subprogram is used to modify data by addition or deletion. With the ’analysis’ subprogram, the metab- olite concentration in the perchloric acid extracts is determined using either the means of all the groups of standards or with separate groups of standards. In the ’collate’ subpro- gram all the information concerning the samples, including dilution fac- tors, patient identification and con- trols, is collated and the results printed in a coherent format. The correlation between the computer determined results and those deter- mined by conventional reading of chart recorder traces is excellent. Considerably less time is required for processing the computer data. French and German summaries will be printed in our next issue. Page 56 Flow Injection Analysis: A new tool to automatize extraction processes M. D. Luque de Castro An overview of a Flow Injection Analysis (FIA) liquid-liquid extrac- tion combination is presented. Its most significant features, its theory, basic components, applications and advantages over other automatic methods incorporating continuous extraction are also shown. French and German summaries will be printed in our next issue. Page 63 An evaluation of the Coulter DACOS analyser A. E. Hurrell, R. A. Hall and J. L. Robinson An evaluation is presented of the DACOS (Discrete Analyser with Continuous Optical Scanning), which has been developed commer- cially by Coulter Electronics Ltd, Northwell Drive, Luton, Bedford- shire LU3 3RH, UK and Coulter Electronics, Inc., Hialeh, Florida, USA. The instrument is a high-capacity discretionary analyser, combining the advantages of centrifugal analysis and sequential continuous operation. Its main feature is that the optical system itself rotates continuously around the samples. It uses semi- disposable cuvettes which are auto- matically laundered. Instrument control, reaction monitoring and data processing, together with test- programming, are available through a dedicated microcomputer. A description is given of the instru- ment and its operation, including the types of assay that can be performed and their calibration. The evaluation was carried out over three months using a variety of methods, and the findings are dis- cussed. Accuracy, precision and linearity were satisfactory. Staff found the analyser easy to use and maintenance was simple. Emergency analyses could be accommodated without difficulty and the small sam- ple volumes required were ideally suited to paediatric work. One or two minor safety problems were identified. Noise and sample-to- sample interaction were initially con- sidered unsatisfactory, but were later improved. The major problem found was unreliability, which should be resolved as software revisions are made by the manufacturer. Given a test: sample ratio of 2:1, the instrument can process up to 1000 tests per day. This would cope with the work-load of a small- or medium- sized district general hospital. With the addition of ion-selective elec- trodes for sodium and potassium estimation, and the provision of bi-. directional interfacing, the DACOS could seriously be considered as a replacement for traditional profiling techniques. Evaluation d’un analyseur Dacos Coulter A. E. Hurrell, R. A. Hall and J. L. Robinson Une (valuation du systme DACOS (Discrete Analyser with Continuous Optical Scanning) qui a 6t d(ve- lopp par Coulter Electronics Ltd, Northwell Drive, Luton, Bedford- shire, LU3 3RH, UK et Coulter Electronics, Inc., Hialeh, Florida, USA, est pr(sentfie. L’instrument est un analyseur discret t haute capacit combinant les avan- tages de l’analyse centrifugale et l’op(ration s(quentielle en continu. Sa proprit principale est que le systme optique tourne en continu autour des 6chantillons. Le systme utilise des cuvettes qui sont automatiquement 45
Transcript of Abstractsdownloads.hindawi.com/journals/jamc/1986/532707.pdf · 2019-08-01 · A program is...
Journal of Automatic Chemistry of Clinical Laboratory Automation, Vol. 8, No. 2 (April-June 1986), pp. 45-48
Abstracts
Page 49
Computer acquisition and analy-sis of data in enzymatic-fluorimetric-continuous-flowmethods for the measurement ofglucose, lactate, pyruvate, ala-nine, glycerol and 3-hydroxy-butyrate in human bloodC. S. Hetherington et al.
A program is described to acquireand process data using an Applecomputer in the determination ofhuman blood glucose, lactate, pyru-vate, alanine, glycerol and3-hydroxybutyrate. The assaymethods are enzymatic and combinecontinuous-flow systems with flu-orimetry. Particular attention in theprogram is given to the necessity tocorrect for the blank fluorescencepresent in perchloric extracts ofblood, although it can also be usedfor a variety of assays where ’blank’runs are not required. Using peakheight measurements the bloodmetabolite concentration is deter-mined by a software package consist-ing offour subprograms, which allowflexibility, although a rigid sequenceof recurring groups of standards,controls and samples is necessary. Inthe ’acquisition’ subprogram, datacan be acquired from three fluorimet-ers simultaneously. The ’amend’subprogram is used to modify databy addition or deletion. With the’analysis’ subprogram, the metab-olite concentration in the perchloricacid extracts is determined usingeither the means of all the groups ofstandards or with separate groups ofstandards. In the ’collate’ subpro-gram all the information concerningthe samples, including dilution fac-tors, patient identification and con-trols, is collated and the resultsprinted in a coherent format. Thecorrelation between the computerdetermined results and those deter-mined by conventional reading ofchart recorder traces is excellent.Considerably less time is required forprocessing the computer data.French and German summaries will beprinted in our next issue.
Page 56
Flow Injection Analysis: A newtool to automatize extractionprocessesM. D. Luque de Castro
An overview of a Flow InjectionAnalysis (FIA) liquid-liquid extrac-tion combination is presented. Itsmost significant features, its theory,basic components, applications andadvantages over other automaticmethods incorporating continuousextraction are also shown.
French and German summaries will beprinted in our next issue.
Page 63
An evaluation of the CoulterDACOS analyserA. E. Hurrell, R. A. Hall and J. L.Robinson
An evaluation is presented of theDACOS (Discrete Analyser withContinuous Optical Scanning),which has been developed commer-cially by Coulter Electronics Ltd,Northwell Drive, Luton, Bedford-shire LU3 3RH, UK and CoulterElectronics, Inc., Hialeh, Florida,USA.
The instrument is a high-capacitydiscretionary analyser, combiningthe advantages ofcentrifugal analysisand sequential continuous operation.Its main feature is that the opticalsystem itself rotates continuouslyaround the samples. It uses semi-disposable cuvettes which are auto-matically laundered. Instrumentcontrol, reaction monitoring anddata processing, together with test-programming, are available througha dedicated microcomputer.
A description is given of the instru-ment and its operation, including thetypes of assay that can be performedand their calibration.
The evaluation was carried out overthree months using a variety ofmethods, and the findings are dis-cussed. Accuracy, precision andlinearity were satisfactory. Stafffound the analyser easy to use andmaintenance was simple. Emergencyanalyses could be accommodatedwithout difficulty and the small sam-ple volumes required were ideallysuited to paediatric work.
One or two minor safety problemswere identified. Noise and sample-to-sample interaction were initially con-sidered unsatisfactory, but were laterimproved. The major problem foundwas unreliability, which should beresolved as software revisions aremade by the manufacturer.
Given a test: sample ratio of 2:1, theinstrument can process up to 1000tests per day. This would cope withthe work-load ofa small- or medium-sized district general hospital. Withthe addition of ion-selective elec-trodes for sodium and potassiumestimation, and the provision of bi-.directional interfacing, the DACOScould seriously be considered as areplacement for traditional profilingtechniques.
Evaluation d’un analyseur DacosCoulterA. E. Hurrell, R. A. Hall and J. L.Robinson
Une (valuation du systme DACOS(Discrete Analyser with ContinuousOptical Scanning) qui a 6t d(ve-lopp par Coulter Electronics Ltd,Northwell Drive, Luton, Bedford-shire, LU3 3RH, UK et CoulterElectronics, Inc., Hialeh, Florida,USA, est pr(sentfie.
L’instrument est un analyseur discrett haute capacit combinant les avan-tages de l’analyse centrifugale etl’op(ration s(quentielle en continu. Saproprit principale est que le systmeoptique tourne en continu autour des6chantillons. Le systme utilise descuvettes qui sont automatiquement
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Abstracts
nettoy6es. Le contr61e de l’instru-ment, de la r6action et du traitementde donn(:es ainsi que les programmesde test sont ex6cut(s 5. partir d’unmicroordinateurd6di
Une description de l’instrument et deson mode op6ratoire est donn6eincluant le genre d’analyses quipeuvent gtre ex6cut6es et leur calib-rage.
L’6valuation a 6t6 faite pendant unep&iode de trois mois utilisant unevari(:t(: de m(thodes et les r(sultatssont discut(s. L’exactitude, la pr(ci-sion et la linfarit6 (taient satisfai-santes. Les employ6s ont trouv( quel’analyseur 6tait facile 5. utiliser etl’entretien 6tait simple. Les analysesurgentes ont 6t6 faites 6galement sansdifficultfs et les petits volumesd’6chantillons requis se prtentid(alement au travail pfdiatrique.
Un ou deux problmes de s6curit(mineure ont 6t6 identifi6s. Le bruit defond et l’interaction entre 6chantil-Ions (:taient consid6r6s insatisfaisantsau dfbut, mais ont 6t6 am61ior6s aucours de l’(tude. Le seul problmemajeur (tait un manque de fiabilit6,un problme qui devrait gtre r6soluaussit6t que les r6visions de logicielseront faites par le producteur.
L’instrument peut effectuer jusqu’5-1000 analyses par jour 6tant donn6une relation analyses/fchantillon de2 5- 1. Ceci correspond environ 5- lademande d’un h6pital gfn6ral dedistrict petit ou moyen. Avec l’addi-tion d’flectrodes ions-s61ectives pourle dosage du sodium et du potassiumet la possibilit( d’interface bi-directionnelle, le DACOS peut s6ri-eusement gtre consid6r( comme tern-
placement pour les techniques tradi-tionnelles.
Evaluation des Coulter DacosAnalysengeritesA. E. Hurrell, R. A. Hall and J. L.Robinson
Die Arbeit evaluiert DACOS (Batch-Analysenger/it mit kontinuierlicheroptischer Abtastung), der vonCoulter Electronics fiir kommerzielleZwecke entwickelt wurde.
Das Ger/it ist ein Hochleistungs-Analyseninstrument, das die Vor-
teile des Zentrifugenprinzips mitdem des sequentiellen kontinuier-lichen Betriebs vereint. Sein Haupt-merkmal ist das optische Systemselbst, das kontinuierlich um dieProben herum rotiert. Es beniitzthalb-weg-werfbare Kiivetten, welcheautomatisch gewaschen werden.Ger/itesteuerung, Reaktionsverfol-gung und Datenverarbeitung sowiedie Testprogrammierung werdenvon einem nur daffir eingesetztenMikrocomputer besorgt. Eine Besch-reibung des Gerfites und seines Bet-riebs umfasst auch die Arten derAnalysen und deren Eichung, diedurchgeffihrt werden k6nnen.
Die Evaluation wurde wihrenddreier Monate, unter Benfitzungeiner Reihe von Methoden durchge-ffihrt und die Ergebnisse wurdenbesprochen. Genauigkeit, Prizisionund Linearit/it sind zufriedenstel-lend. Das Betriebspersonal erachtetedes Ger/it als leicht bedienbar, unddie Wartung ist einfach. Eilanalysenkonnten ohne Schwierigkeitenberiicksichtigt werden. Die erforder-lichen kleinen Probenvolumina eig-neten sich ideal fiir paediatrischeArbeiten.
Ein oder zwei kleinere Sicherheits-probleme wurden identifiziert. Sig-nalrauschen und Wechselwirkungzwischen Proben waren anffinglichungenfigend aber wurden sp/iter ver-bessert. Ein gr6sseres Problem stelltedie ungeniigende Zuverlissigkeit dar,die jedoch durch Software-Ueberarbeitungen des Herstellersbeseitigtwerden sollen.
Bei einem Verh/iltnis von Analysenzu Proben von 2:1 kann das Ger/itbis zu 1000 Analysen pro Tag durch-ffihren Damit kann die Arbeitslasteines kleinen bis mittleren Regional-spitals bewiltigt werden. DurchZuffigen von ionen-selektiven Elek-troden ffir die Bestimmung von Nat-rium und Kalium und die Einrich-tung eines bidirektionalen Interfacekann der DACOS ernsthaft ffir denErsatz traditioneller Profiltechnikenin Erw/igung gezogen werden.
Page 70
Automatation of a flow-injectionsystem for multispeciationJuan Ruz, Antonia Torres, AngelRios, M. D. Luque de Castro andMiguel Valcfircel
An automatic flow-injection system,which is based on the integration oftwo detectors (photometric andpotentiometric), is presented. Thesystem allows absorbance-pH data tobe collected from every sample bymeans of an on-line microprocessor,which, with a suitable calculationprogram, provides the concentrationof up to nine species of chromiumpresent in the sample. A ’reversed’configuration and the asymmetricmerging zones mode are used todiscriminate between the differentoxidation states of chromium.
French and German summaries will beprinted in our next issue.
Page 75
Evaluation of derivative spectrafor the selective determination ofdrugs: quantitation of theophyl-line with phenobarbital and light-scattering componentsP. B. Arnoudse and H. L. Pardue
This paper describes the evaluationof zeroth-, first-, and second-derivative spectra for the quantita-tion of drugs in mixtures. In parti-cular, single-wavelength and multi-wavelength data-processing methodsare compared for the quantitation oftheophylline in the presence of phe-nobarbital and a light-scatteringcomponent. For 0-25 mg/1 oftheophylline in the presence of vari-able amounts of phenobarbital andthe light-scattering component, sin-gle- and multiwavelength second-derivative data yielded least squaresequations of computed (y) versusprepared (x) concentrations of y1"00 x + 0.52 rag/1 andy 0"98 x +0"04 mg/1, respectively, with stan-dard errors of estimate of 0"32 and0"04 mg/1. First-derivative data yieldslightly larger intercepts and stan-dard errors and absorption data yieldmuch larger intercepts and standarderrors.
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Abstracts
Evaluation de sp6ctres d6riv6spour la d6termination s61ective dem6dicaments: quantification de lath6ophiline contenant du ph6no-barbital et des composantes dis-persant la lumi/reP. B. Arnoudse and H. L. Pardue
Cette publication d(crit l’(valuationde spctres ainsi que de leurs pre-mieres et secondes drives pourquantifier des m(dicaments dans desmixtures. En particulier, desmfithodes de traitement de donn(espour une longeur d’ondes fixe etmultiples-longueurs d’ondes sontcompar(es pour la quantification dela thfiophiline en prfisence du pheno-barbital et d’une composante disper-sant la lumire. Pour 0-25 mg/1 deth6ophiline en prfsence de quantit(svariables de phfinobarbital et de lacomposante dispersant la lumire, lessecondes d(rivatives pour longueurd’ondes simple et multiple, don-naient des quations de moindrecarr(: pour le signal en fonction de laconcentration de y 1"00 x + 0"52mg/l et de y 0-98 x + 0"04 mg/1respectivement avec des erreurs stan-dard pour la valeur estime de 0"32 et0.04 mg/1. Les donnes bases sur lapremiere d(rive montraient desvaleurs d’interception et des erreursstandard l(grement plus (:levfies etles mesures d’absorption donnaientdes interceptions et des erreurs stan-dard beaucoup plus 61eyries.
Auswertung von Ableitungsspek-tren fiir die selektive Bestimmungvon Drogen: Quantisierung vonTheophyllin in Anwesenheit vonPhenobarbital und lichtstreuen-den KomponentenP. B. Arnoudse and H. L. Pardue
Diese Arbeit beschreibt die Auswer-tung der nullten, ersten und zweitenAbleitung yon Spektren f’fir dieQuantisierung von Drogen inGemischen. Im besonderen werdenEinwellenl/ingen- und Multiwellen-1/ingenmethoden der Datenverarbei-tung fiir die Quantisierung vonThe6phyllin in Anwesenheit vonPhenobarbital und lichtstreuendenKomponenten verglichen. Ffir 0-25mg/1 Theophyllin in Anwesenheitvon verschiedenen Mengen yon Phe-nobarbital ergaben die Einwellen-l/ingen- und Multiwellenl/ingendaten
der zweiten Ableitung die Regres-sionsgeraden y 1"00 x + 0"52 mg/lund y 0"98 x + 0"04 mg/l fiirberechnetes y gegen hergestellteKonzentrationen X, mit Standar-dabweichungen von 0’32 bzw. 0"04mg/1. Die Daten der ersten Ableitungergeben leicht gr6ssere Achsen-schnitte und Standardabweich-ungen. Die Absorbtionsdatendagegen ergaben wesentlich gr6ssereAchsenschnitte und Standardab-weichungen.
Page 80
Evaluation of fluorescence excita-tion transfer immunoassay for themeasurement of plasma cortisolJ. Calvin et al.
Fluorescence excitation transferimmunoassay is a suitable techniquefor the measurement of plasma corti-sol. The reagents, once reconstituted,are stable for at least three months.The method shows no interferencefrom bilirubin or lipaemia up to highlevels. Haemolysed specimens givefalsely raised results and prednisol-one causes significant positive inter-ference. The precision of the assaycompares favourably with the radio-immunoassay used routinely. Theassay is simple to performand reliable.Comparison with anHPLC techniqueindicated a significant bias; reflectingthe positive bias seen with Advanceresults in external quality assessmentmaterials when compared withGCMS target values. Comparisonwith RIA yielded a wide scatter,although the regression parametersgave a slope of0"97. The discrepanciesare probably due to differing specifici-ties ofthe antibodies employed.
Evaluation de l’immunoassay partransfert d’excitation de fluores-cence pour le dosage du cortisolde plasmaJ. Calvin et al.
L’immunoassay de transfert d’exci-tation de fluorescence est une tech-nique convenant pour le dosage ducortisol dans le plasma. Les rfiactifs
reconstitu6s sont stables pour aumoins 3 mois. La mthode montreaucune interffirence due t la biliru-bine ou t la lipmie mme t niveauxfilevs. Des 6chantillons hmolis6sdonnent des r6sultats trop filev6s et laprednisolone cause une interffirencepositive significative. La precision decette mfithode de dosage se comparefavorablement avec le radioimmu-noassay utilis en routine. Lamthode est simple et sfire. La com-paraison avec la technique de HPLCindique une dviation significativereflettant une dfiviation positiveobserve lors du contr61e de qualit6externe pour des mat(riaux com-par6s avec les valeurs GCMS. Lacomparaison avec le radioimmu-noassay montre une grande disper-sion bien que les paramtres der6gression donnent une pente de 0"97.Les discr6pances sont probablement"dues aux spcificitfis difffirentes desanticorps employfis.
Auswertung der Fluoreszenz-Anregungsiibertragung-Immunomethode fiir die Bestimomung des PlasmacortisolJ. Calvin et al.
Die Fluoreszenz-Anregungsfibertra-gung-Immunomethode ist einegeeignete Technik f’fir die Bestim-mung von Plasma-Cortisol. DieReagenzien, einmal hergestellt, sindf’fir mindestens drei Monate stabil.Die Methode zeigt keine Interferenzmit Bilirubin oder Lipaemia, auchbei hohem Spiegel. HaemolysierteProben geben fiilschliche iiberh6hteWerte, und Prednisolon verursachtsignifikante positive Interferenz. DiePr/izision der Methode vergleicht sichgfinstig mit der Routine-Radioimmunomethode. Der Test istzuverl/issig, und einfach durchf’fihr-bar. Vergleiche mit einer HPLC-Methode ergaben eine signifikantesystematische Abweichung. Ein Ver-gleich mit RIA ergab eine grosseStreuung, obwohl die Regression eineSteigung von 0"97 ergab. Die Diskre-panzen wurden wahrscheinlich durchunterschiedliche Spezifit/iten der ver-wendetenAntik6rperverursacht.
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Abstracts
Page 85
The influences of the between-and within-run components ofvariation on the mean ruleP. Douville et al.
Previous work with a specific errormodel has indicated that there isdegradation in the performancecharacteristics of the mean rule whenit is used for the quality control ofanalyses with significant between-run variation. The selection of theerror model can markedly affect theperformance characteristics of themean rule. An alternate error modelfor the mean rule is proposed, whichmore realistically simulates its appli-cation in the clinical laboratory. Thismodel indicates that the mean rule’sprobabilities of error detection andfalse rejection are not compromisedby significant between-run com-ponents of variation. The use of themean rule is recommended for thequality control of analytical methodsusing multiple controls regardless ofthe magnitude of the between-runvariation.
L’influence des composantes devariation entre et intra-s6rie sur lar/gle de la moyenneP. Douville et al.
Un travail ant(rieur avec un moduled’erreur sp6cifique nous a indiququ’il y a dfigradation des caractfiris-tiques de performance de la rgle dela moyenne quand elle est utilis6epour le contr61e de qualit d’analysesavec variations significatives entresfiries. Nous avons trouv que las(lection du module d’erreurs peutaffecter les caractristiques de perfor-mance de la rgle de la moyenne.Nous proposons un module d’erreuralternatifpour la rgle de la moyennequi simule de fagon plus rfialiste sonapplication dans le laboratoire cli-nique. Ce module indique que laprobabilit( de dfitection d’erreurs dela rgle de la moyenne et d’unerfijection fausse ne sont pas compro-mises par les composantes de varia-tion significatives entre sries. Nousrecommandons l’utilisation de largle de la moyenne pour le contr61ede qualitfi de mfithodes analytiquesutilisant des contr61es multiplesindfipendamment de la grandeur desvariations entre s(ries.
Einfl/isse der Variationskom-ponenten innerhalb und von Seriezu Serie auf die Mittelwerts-RegelP. Douville et al.
Fr/ihere Arbeiten mit einem spezi-fische Fehlermodell deuteten an,dass eine Beeintr/ichtigung der Lei-stungsffihigkei der Mittelwerts-Regel eintritt, wenn sie f’tir die Quali-t/iskontrolle von Analysen eingesetztwird, die von Serie zu Serie signifi-kante Variationen zeigen. Wir stell-ten fest, dass die Wahl der Fehler-modelle deutlich die Leistung derMittelwerts-Regel beeinflusst. Wirschlagen ein anderes Fehlermodellf’tir die Mittelwerts-Regel vor, dasrealistischer die Anwendung im kli-nischen Labor simuliert. DiesesModell zeigt, dass die Wahrschein-lichkeiten der Fehlerdetektion undder filschlichen Rfickweisung durchdie Mittelwerts-Regel nicht durchsignifikante Variationskomponentenvon Serie zu Serie nicht kompromit-iert werden. Wir empfehlen die Mit-telwerts-Regel ffir die Qualit/itskon-trolle von Analysenmethoden unterVerwendung von vielfachen Kontrol-len unabh/ingig von der Gr6sse derVariationenvon Serie zu Serie.
ANALYTICON 86
Analyticon 86, to be held at the Commonwealth Institute in London 23 to 25 September 1986, is being upgradedfrom a 10-session meeting to include a total of 18 sessions plus poster displays. More intensive coverage ofmoretopics of direct interest to practising laboratory personnel will be included in this three-day multidisciplinarymeeting. Speakers are being invited from Europe and the USA to present the latest advances and concepts inlaboratory technology.
A special seminar session on laboratory robotics will be presented by Dr Grover Owens of the Proctor andGamble Company, USA. The emphasis in this session will be on users’ experiences and particularly withsmall-scale systems.
Another topic area of sufficient importance to warrant two sessions in the Analyticon 86 programme isspectroscopy. One session will be on multi-component spectroscopy, the other on atomic spectroscopy. Anotherspecial seminar will be presented by Dr Bernard Vandeginste from the University of Nijmegen in TheNetherlands on ’the analysis of mixturesmchemometrics for data analysis in liquid chromatography with diodearray detectors and for multicomponent analysis in diode array spectrometers’.
There will also be specific sessions on chromatography within the programme. I is intended that these will deal infurther depth with diode array detection and with biochemical applications of HPLC.
Further information from Scientific Symposia Ltd, 33-35 Bowling Green Lane, London EC1R ODA. Tel.: O1 837 1212.
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