2013 11-13-SoftwareSustainabilityInstitute@Manchester

Ant genomics & Bioinformatics for emerging

model organisms

@yannick__ http://yannick.poulet.org

mailto:[email protected]




Animal biomass (Brazilian rainforest)

from Fittkau & Klinge 1973

Other insects AmphibiansReptiles

Birds

Mammals

Earthworms

Spiders

Soil fauna excluding earthworms,

ants & termites

Ants & termites

454IlluminaSolid...

This changes everything.454

IlluminaSolid...

This changes everything.454

IlluminaSolid...

Any lab can sequence anything!

Major questions

Major questions

Which genes are involved in social behaviour?

Major questions

Which genes are involved in social behaviour?

Which genes make it possible for queens to live up to 30 years?

We need great tools.

Tools for genomics work on emerging model organisms


Antgenomes.org


Antgenomes.org

SequenceServer.com BLAST made easy

CTTTTTtGGTCGAGGTAACCCACACGTCGGGGGgTACGGTTGTATACATTTATGGGAGTAGCACGCTGACCCATAAACATTACTTAGTAGCCGTGCGACATAAAAATTGCCACTCGCGTATCTCAGGTGGTAGTAGTACTCTTAAAAAGTGTCAGCGTGAAAGTAGGCCAAATTACTGCATTTCCGTGTACACGTATCGTAACGCCTTCTCGCGATAAAAGAAGAATAAAATTTTCTCCTAAATATATATGATACCGCAAATGGATTTTGAGTTAGAGAGTAAGATGCAACGAATTGAAATTTATATAAATAATATATGATATTTTCTTATAAATCTTACTAAAtATTTTTTTTGTCAATTCTATCAGAATATTTCGAACGCACGTTTATCTGAACTGAGACGGCGGCATCAATTAGAAGTATTGTGAACGCAGTTGAAATGTATATCCATGAGAAACTCATACTAACGCAGAGCAGTGAAAACGAACACTTGACTCAGGTTTAACCGCGACGAAACGGTATCGGATAATGGACGGGACGAAAAGCGAATTCGACGGCTGGTATCGAGTTACGTGAAGATGTACCTCCGTCTTCGTCGTCATTGAAATTGACCGCGATACGCGAGGTTCACGGAGAGAGACAGAGATCGTAtCTTCCATCTTCTAAATTTCCTTGATTTCTCGTTTATACTCGCGGCAGCAGCCATTTACTTGTTCCACTTGGCGTTCTCAATTTTGCGCTTAGGGAAACAATTAAACCCAAGGATCTCGCATTACATTTTTCCTCCCGCATCTCTACATGTAGCGTATTAAAACCCCGTAGATGCCCGTTCATGCAGAACAAACAACACGGAGCAACATTCGTGCTCTATTTATTTAAGACTCTTATCCAAGGGGGATGAGTTGCAGCGCAGCGAAACTGGTCTGGTATTTATAGTATTTGGATTATGGAATAATCCCTTGCATAGCGACGGCGAAAAGGGAAGGAAAGCCAGGAATGCACGGTGTTTACCGATATCCTTCACCTCTTTTACCTCTTCGTATTCCTCTCTCTCGCGTTTGCCAtCTTTTTTTTtCTCTAAGCGATCTCATTGGTACCGCACGAGGAGCTTCAATCTTTTCTCTTATCTGGATTACTCGTACACAGAGAGTTACAATCCGCTATAAATTCCATCTTTTAAACGCGAAATTCATTCAATTTATTTGCGACCTCAATCATTTTCCCGTGAGATCGACCGAAAAATTCTTAATAAGGATTTATTCGTTTAATCGCGAGCTCTGATAATTTCATTACTCATTGTAACATAAGCGCATTGATATGAATGTACGTATACTGCAAAGTATAGAAAAaTAAATCCGCGTTAAAGTTCTAATTTTTGAAACGTTTATTATTGCTCGTAGAAAAGAAATATTTGTATCTGTGAATACATGTGTCGTAATATTTTAAAAATTAATTCTTTACCTTTATCACGAAAGCTAATTATTTACATATTTACAAATAAACAAAAATATTAGCGTACAAAAATAAGTAAGAGAAAAGAAATTAATTTTGTCTTGGACAAAACGTTTCATTAATGTCGTATTATGTAAAAAAAAATTTAATAAAATGTCAAGATTTTATTTTTGCGAAAGAATAAAAACAAGAGCTTAAGTTCATTCTTTTTGATAGATAATACAAGGTTCTATTTTTGATAAAGACTCATTTTTAGAGGGTTTTCTTTTCACATTTTCCGCGGTACGACTACAATGCATCGTCGAAATACAAAACAAAAAGAAAAGAAAAAGAAATCACAGCGCATCTCGTGCAAAAGCTGCGAACAGTCTACCGTCTACAAACATCGTCAGGAAGATTCTTTCATCTGGTCACGGGGGAGACATCGTTCGCGCAGGTATCTCTCACCGTTGCTGTATATTCATACGTATCTTCGACCTAGTCAAACAACGATTCGACCCAGCACAAAGCAGAGCGGGCGTCAGCGTAGCGTGAAAATTTAACCCGCGACTTGGCGGAGATTTGATTTTTTGGCGTCGGTCAAAAGGCAGACGTGCCCTGATATATCCGCGACGTTATTTGATGCACGGCTACCGGTACAATTGGACTGAGGTAAATAAGAAAGCAGCAACGACAAAAGACTTCCTTCTGTGTGGATGTTAGTCGTACCCTTGGGCGTTGTCTGCCGCCGTATCTCCTAAGTTGGTCGGGGAACGCGGAGTAAGGTTGGACGAATCCTAGTAGATAGCACAGCTGTATTAAATAAATAGAGAATCGCCGAGGCTTTCGACGATGTAGGAGGAAAGAACCGTACTCCTTGAAAGTGGAAAAGGTTGAAGTCAAGCTCCCCCCTCCCTCCCCTCTTCTCCCCGAGAGAACGTCGAGAGGAAGCGTCGTGGATGGAGAACAAAAGTCTGCGCGAAGAAATATCAGAAAAGTCGAACTAATATTTGAGATTACCCATCAATGCTTTTATCCGTCGTGTATCTCCGAAGAAAAAAAGATGGGTATAATTTTAAAAATACATTTGTCAGTGTCTGATATGCGCCGATCTTCTTTCCTCCATGCATTAACGCTTCGTGCGGATACGACCGGGCGTAATCTTAAAAAAAaTGATCGCCCATATACTAAACCACTGACAGCACGTACAGTTCGCCGAGAAGTTTAAGACCACTTTCAGCGCGTTAAACTTTTACACTCTAATTAGAAATTAATAAAGACTAATAGCTTTATCGTCGGTCTATTAAATCCCGTGGAGGAGGTGCTGGAAGTTAGGCAGTCGCGAAGGTGGTCGGTCGGTACACTCGGCAATAAAAGTTTTTTtCTTCGTGGGATAACGGGGACTTTAACTGCAAGAAGAAAGAAAAAAGaGGAAAGAGAGAAAATTCGTTGAAAGCGAAAGTCGAACTACGTTATTTATCTGATGGCCGACACTTCGAAGATTGTTGCGCGGTGTGTCGACGCAGCGCGCAGACGCTCACCAGACGTTTTCTTTTCATTTCTTATTTCTCCCGTCGAAAAAGGaGAGAAAAGGAGAGGATCGGGACGAAGTGAGAAAGAGTAGCGATAGAACGTCGGAAAGGGGAGAAAAAAaGAAATCAATGAGCGGAAACGTGATACGATGGGCGAAGGGCCAGGATGGGGGACAGGGTCGGCCGTGCTGTAGTTTCTCTATTTCCTTCATTAACTCTGATTCAACAGCAGCGTAACTTTTAATTGGAAGTGGTGGGGGgTCGAGTCGAGGCTCATTTCCCGGCGGACTTCCCCATACTGGAATTCAAGCCAATCCCCAAGCGTCTTGCGAGAGGTCACTTCATTCGTTTCGATATTCTCAGATTTTACTTTCATAGCCGTGCGATATAGCATTTGAATTGCTTCGAATTAGAAACGGTAGCGCCTATCtGTTTTTTTTTtAGAGATTAAAGAGATTAAAATCTATAGAAACGTAGTTTTTCGACAGATAAATGCTTCTTTACTGATGTCACATATGATACGCAAAGGGAAATAGATTATAATTAATGATTGTACAATATTTAGTGATATCCCTAACAATAATCATCACGAATGAATAAATATGAATGCATAAAGCGATGTACAATGGCACATTAATTTCCTATCCATTATAAATATAATAAATGTATAAATTGTATTAATGACGAGAACGGGAGATGCGCTGATTTAATTTAAATAAAATCACCAGAGCGTCATCTTCCAAAACGGAAAATAACGGAAGCGACAGATGCTTTCGAAAGCGTCCGTGCCTCCGTCCTTTACTCATATCCAGATAAAGGCACACGTGACGCTCGTAAGATACTGCCGGAAATAAGAGCGATAAAAAGCAAGGCGAATAGTTACACGCCGGGCGACGAGGtCCTTCGAAACGCGAAAATGACGAGCCTGCGATAAAGGACCGCCATCGCGAACGGTAACGGGAGTAACCCCGTGGAATTTAGTATGATCCCCCGCGCACGGCACATCgCGATACTATCGGTTTCTCGTTACCCAGATCGGTATATTGTTATTTCAGCAGTTCTCGGACAAGGTTCACGGACATTCGATAGCGCGAAGGGGACCGGAGCTCCTGAAGAATTCAACGTAACTGGCCGATAATCGGCCTCGTAACGGCTACCCATTTTCCGCGGCACGTCTCGGGGCACCATCATCGAAACGACCTTATTTCCATGCATCCACGTTGCACCTGCTATTGCACCGTAGCCGTCACCGAACTATATCGTCGTCGAACTTATTCGATGTACGATAATCCTTACGGAAAGCTTTAAACAGCTACCTGCATGCATACTAAAGATACGTCCTTcGCTTCTGGGATCCACCTTGTATTCGCGAGACATTGCATAATTTGTATTCGGTGTAAATATGCTATCCGCGTGGCGATCGACCACCGCGAACCGCGCGGAAACTCCACAGGCGGTGCTTGaTAACGTTCCGCGAACGCAGATAAGCAAATGCCAACTTATGGCAGCAGACACTcTACGCGACTTAACAAAATCTCGTATAAACCCGAGATTTTACATGGCAGTTTAACCAAAATAAATTAAGAGAGAAAGACGGAAGAAGAGAGACAGACGACGTGCCTTGCGTCGTCGTAAGAATACGTAAAACCGTGAAATGCTCGAAATGAAGCCGTCCGtATTTTTTGCATACGCTTATATCCGAAGGGCCGCTCTCCCCTCGTAATTGCGTGTCCTCGACAAAAATCTCCTTTCCGATTTGCCTCTTAGTGCTCGCGCATTCCGTCCGCGTACGACGCTTGCAAATCGGCTGCATCGCCGGCGTCACCGGCAGAAAAACGGAGAACTTTCACGGCGAGTCTCGCGGAGCCTCGTACGGCAAAACACCGCGACGACGCCTGCCATTTTGGGCTGGCACGGGAACATAGCAGGGGCCAGGATGACGGAGGGGTGGTGATAGGAAACCCCGGGCAAAAAACaGCGCCTGCAGAGATCCGCGACGCGGCTGTTTAATTCGGGACTTCGCCCGGGGGAGGAAAAAAAaGGGCCGAGGCCGCCGGCTCCTCCTCGCGCCGCTCATGCATTATGAATAATGGAATTTAAATATACGCGAAACAGATCCCCGCTGGGCGAATTCGGCTTAGAGAGGTGGAGTGCAGCCTGGTGCCACGGTGTCGTGTTCGCATTGTTAATTGGCCGGCCGTTtCATTTtCCGTTGCGGCGGCGTAACGTGAGAGACAGCGTAACGGGAAGCGAAACGACACGGCGCGTCGAGCGTGCACAGAGAAGTTGAATTATCGAGGGaGAAAAAGGGCGCGAGGGAGGGGCAGGACGGTCAAACACTGAGAAAGCAGGCAACATCCGCGAACCCGTAAATTAATTAGGGCCAACATAATAGCTGGGAATGGCTGGTAGTGGTGGTTCGTCTTCGTGGTCCAGCTTGCGGTTCCCGAGTGCCCTCGCAGTTCGCGCGGTCATGCCTCGGAAACTACACGATACACCTTGCGTCATCCTTACACGAGGATACCTGTTTGCGATCCCCTGAGGTATTAACGAGCGTATAAGCAGACTCAAAGTACGAACACACTGCTAGTTTCGCGTGCATGTTGCTCGTCGCCGTGCAGACGAGTTAGTACTGTCGACAAAGTAGTCGTAAAGTACGTAAGAGTCTTCCTCTAGTAGCGAGGACTAATCTCCCGGTACATTAATCTATGTATATTTTATGATATACACACCTGTATTTATCGAAAGTTTATGTTTATCTCGAAAATTAACGTTAATTTTCGAGGACGCAAAAGCTGTCGCAAGTTTaTTTAATTTTGTTTGtATTTTTTTTTCTTGCTCATTTTTATTTCGAAGTATGCAAATTGAATCTCTTGGTGGCATAACGAGAATTTTCGAAGCTTAAAGGGATCTGCGTTGGCGCGAAGAGAATGGGACTTGTTTTAAACTTTTCTTTCCAACGAACACCGTTTCCTTCATTGTAACGTAAAAATGGTGAGCTTCTGCGGCGCGATTGGTCTCATCTTTCCTCTCGTATCGTTCGTCATTTTGTCCGAGAGCGTGTAGATATTGTACCCGATAGAACGGAGACGGAATCACGCTATACGGTTCCCTCCCAAATGTTCGTTCCTCACGGGCAAAAGTACAAGTAAAAGTAGGAGGGTCCGACTTTATGACCCTACGAGGCACAATAAAGGTTGATGACTACTAAACGTAGAAGAACACGTATACAGTCCGACGTAAAGTTATTTTTCAATTCCGCGGTCCTCCGCCGCAGTCGTTTTGCCTTGACGTGGAAAAGGAAATTTCCCGGGTTCTAGTCGTCCGGTCTTCTTTtCGTTCTACTGACAATACCATAtTTTCGACATAGATCGATCTCCTTTtCTCTTtCTCTCTCTCTCTCTCTCTCTCTCTTGAAAATGAAAGCGCGGAAGAGCCGGATCGTGCAACGCACAATGCGAGGCCGCTTTGTACGAGGTACGAGGAGTCCGTCATGCGCCGTAAAACGCCGGAATATGCAATACTACTTTCGCAGCGACGGGGCTTGCACGAAGTAATATCGTGAAACGTAGAACCGTTCTTTTCATACGGATATGCGGGAGAAGTTGCTCGTCCGCCCTCCGGCGCATACACGTCGCGCGAGAGTATCGTGCATCCGAAACTCTGAGATGAAACTCTTCTGTAGTCGATATTCGTCGCGATAAATAGATAAGTCTCGGATAAGGTAGAGACATCGATAATTCCGTTACGAGAATACTCGAGAATAAGATCCAAGTGAAGTGATCACGCTCCATATGGTTTCAGATTAATCTCCAATCGGCTGACGAAGGAGGATCATCCTTTTACCGGTGGAAAATAGGGTGGATTGGCGGAGCAAGAAGGCTAAACAGAGAATAAACAGGAGCTATAGCCGAACGAGGGAGAAGGTAAGTAGATGCCTCGGAGAACGTGAGCGAAAAAAGGAAACGGGTACGAGAGAAAAAGAAACGGACGGAATAGCGGCTCGGGATTCGCATCCCAAGGAAAACCAAGGCTATACCGGGGTCTTGGATTATTCGAGGCACGACGACGATCTTCGAGTCAGCCGACTGTCTGTACCGTGAAAGTGGCACGTATCGATCGCACGGCTGGATTATCTTCCACTTCGATCTACGACGATTACTTCCGCCATCGTATATCCGGGCTTTGCACTAGCGAGGCTATTTAAAAATCTGCGCTCAGTAACTACTTATGATTTTTCCATCAGAAACGATTGTGGAGAGAAAAGAGGGAAAAAAAGAGATAGACAGCCTCTGGCTCGAAATGCTAATTTCGCAATCGAGAATTAAAATGCACTTCTTTGTATCTAAATTTTCGTAGAATTAAAATAAAATTGAATAAAGCAATGAATAAATTGAATAAACTAAAATATAGCTAAATATTTTTTCCTCTATACAAGGTGAATATAATTATCAAATATTTAAGTATGTAGATTGAATTTAAACAGCCTCGGAAGAGAAAAGAATCGGATAAACGAAATGCTTTTGCCTCTATTTTCAAGCACGTGACGAATAAAATCTAGCAAAGCTTTTCGACACAATATGTCGACGCAAATGTGGTCTATTTTGGCTAATGATTATTACCGGGAGTCCCGGGCACGGTGTGTCCGCGGCGCGATAAATTAGAGCGCGAATCGACTTCCACGGCCGTTGTAGAAGGTACTTTGGCAAACGTTATTTCTTCCTGTCTCGAAGGAAGCGCCACTCGAAAACTTGGAAAAGTTCGGCCAGCTGCACGCACCGCGATCTCGGGTCCGTTCTGGCTCGGTGGCGTGCGCAGGACGGTTGTGAGACGAGAGAAAGAGAGAAAGGAGGATAGAGCGCGAGAGGGAAAGAAGAGGGAACATCCGCGTGCGTGGTTGTATATGGCGTTAATGGCGGCGAGCATAAAGCATCCCCGcGCCTGCACGCTCGACCACGGTCGTTTACAGTGCCACTATTTATGTTGATAACTTCGGAATGGAGTCAGATACACGGTACCGAGTGCCGGCGGTTCGGCGGTGGTCCGGCAGCGGTCTGGTAGCACTTGCAAAGTATGGGAGAAGAAGGGGGATGGTGGATTCGCGGATCTTTTTGGTCGTGCAAGGAAGGCGGGTCGGTTAGGGTAGGTAGAGAGGAGACGAGCCGAGTCGAGAACAAGATTCAAGCGGAAAGTTTTGCGAGTTAATGGTGCGGAGGTAATGCCGCGGCCGCAACAGCAACAGCCATCCCCCTGCTTGTGTGTTCGCTCGCTCGCTCACTtCTTCCGTTCTCTCCTCTTCGCGCTAGTTCTCTCTCTCTCTCTCTCTCTCTCTCTGtCTTTCTTGGAATAGCCGTAGAAAAAaGGAAAAGAAAGAGAGAGAAAGAACGAGACTCCTTTCTCCCCACGAATTCTCTCCTCTCTTTAAGCACACTCTCTCTTCCTGCaccCCCCCCCcTCcTCTCTCTCTCTTTCTCTCTCTATTTTCTTGTCTTCCTCACTTGCATCATCCCTCGTCCATTTCCcTCTGGAGAACGTGCCACGTTCTCACTTCCTCGTTCGTCGCTACTTTTTTTCTTCTTTAGTTGTCTCCTCTCACACCCTCGAGACGGCCCGATCTTTTCCTCGTACAGCTCCTATCACGAACAGTCGCTAACAAGTGCATCGAATGCAAGTTGCCGACAAACTTCTTCCACCGATTTGTGCTTGTTCTCTGTGCATGCGCGGGCATGTATATCTCTATTAGGCGACATCTGCTCTCAGCTTTTTCATAACGAGGTAGCGCGGATTCGCGCTCCGAGAGACTTCACGAAGCACTTCCGATCTCGCTACAGTAGAATGCGTATTGTATTTTCTTGTCTCTCTCATTTACTCTTTTCTATCTTTCGTATCTAGCGTGAATACTCCCATGAGGAATGTAGAAACCAGATTTTGAAACGGCTTCTTCGTATCTAAATTTTCTTAACTATTTGTTCCTCAAAGTCCATTTTGACGTTATAAATTTTTATTTTTTAATGAGAATGTTTTTATGTGGAGAAGAAAAGTACAACTTTTTTCAAAATGCAATTAAATTTATACAAGACTACTAAGATAAAAAAAGATGCAAATAATAATGTTCAACTTACTAACTGCTATATTATTAAGGCCAAGTTAAATTACAGATTTATGATGTTGCAGAATATAATAGAAAAGTTTCAAGAAAAAAaCATTTTAAAGCTTAAAGAGTTTGTTTTCAAACCCATCAAATTTTTTtCTCTGGCAGTGCTGCTGCTCTGCGACGTCATTTTCAAGTCGATCAATGCAAAGTTAACAAAAGTATTTCAACTTTAAAATCATGCAGAAAGTTGGGAAAAATTTGTTTTCAATTTTACTTAAATTTTAGTTACATTTTTTCTAAAAGCTTAAAATCTCCTCTTTAAAATATGTCTTTAAAAATTGAGCTATGATTTTTCTTCACCGAGTTATCGTAATTTGAAATGGCCAATCGACGTTTTCTTTTCATCACTAGGAATAATGCCGGCGGATTTAAGCTTTCAGAAGATTCCAAGCAAAGTTGAAAACAATTTGTTTGAATTCCGCATGATCTCAAAGTTGAAGCTAAAAAAATTTCACAAGACTTTAAAAGACAAAAAGTCAATGTTGCACAATCGACTTGGAAATAACGCCACTTGGAGCAGAGAAGTATTGCCGAGAAAAAGCTTCGACCGGGTTTGAAAGCAGGATCTATAGGCTTCCAAACTTTTTTTTtGAAAAATGGAGCTTTAAGGTTACTTTTCACGAAATTGTATAAATTGTGTTTTGTTGtTTTTATCATCGATTCGAAATGATCCTTTCATTTTGTTCAAGAATGTATCATTAACTTAATGCAATGATATCTTTTAATAATTCAATACTTCTTACTAATTAGATTTAGAAAAGTTTCATGAACAATTTTAGAACGGTTATTGCTAATTATTCGCCAAAAACAGACGCCTTTCTTCGAGGAGTAACAACTCAAGCTGCATTCGCGGTTGACGGCTTTCCGCGGCGCGTCGCGGTAATATGGCTCCTATTTAATTACCCGGTCCTTTGGGAGCTTAAACCAGCCAGAGCTGGAACGGCTGCGCCGTTATTCTATCTAGACTCCTTTGCTTTCTCAAGCAGGCGCGCGATCAAACCTTCTCGCATAAAAGACAATCGCAGCTGGCAGTCGACGACGCGcGGGACAGTCGAATCATGACCCGCTCCTCTCTAGTTACCGCCGTCAGTCTGCTCTACTTCCAGACGCCGCGCGTAATCTATTCGACATTAGTTGCTTGATTGCACCGTAAAATGCGACGGCGACGTGAACGAGAACGACGACGACGACGACGACGACGACAACGACGACGACAATGACAACGACGACGACGACAAGAGTGGGTTCGTCGGTGCTCGATGGCGCCTCCGATTTCAACCGCAGCACGATGCAAGCCCATTACTATCGCCCGGAGCTAAACGGCACCCGGAGCTCGTGCCATTAAGGGAATCTAGGGTCCGATCCACCTCATTGAATTCCGTTcAATTGCGATTATGATAATGCGTGAACGATCGCCGTGGACGTGAGCTACGGaCAACgAGGGTGTTCGTTCTCGGCTCAGAGAAACGCAGCGATAAAATTATCAGTGACAGCTTCATTTTTGTTACATTTGACGTTGAAAAATTTGCGAAAGAAATGTGCATTATTCAACTACATTGACAATTGTAAATCTTACATGACCTTTTATTAATATACATATATGTAAGATATATGTTCCATCTCTAGTCTCGTTCGATAATGAAGAAATTATAGCTGCAACATAGACGCCGGATTATTCGCGGGGGAAACCGTTGATTATTTGGACTCCGTGGGCTCGGGGTCTGGTTTACTTCTTCCCTTATGTCCGGAGATAATGGACACTATTACCTTAACGAGGCCCCAGTCTCTTAGCCGGTAATTGCTTCGAGATATCCGAGAGAGCTCCGGCGTACGTTGCCGCCTGGTGTTGCAGGCAGAGAACCCaGACGGTATTATTGCCGCCGAGGCTACTCGCCCGTTTCATGGTGGTTATTGTTATGGGCCTGCGGCATTAAGATGTACACCGCACTCTCATACGGGACCCACCACCCCATATACGAGCTGCATATATACTTATGCGGGGAGGATTTCATTACGCCGCTTCATAACTGGCGGTCTTCAAAATCGCGATAAATCGGGATTGTCTTCCTTGTTAAATCAGCTCCCGTTCCCCTTCCTCATATCGCTGACGAAGCCAACGAGACGGATTAATCTGCGCGATTGAATGGCCTCTATGACGTAAGCGCACCGTTTACTGGCACGGCTCCTCGTGTTCACGTGAAACGATTTGCGTCGTAAATATTTTATTTTAACATCGCAACATCAAGACAGACGAGGATCGGCTATTGCCTCGTGATCCTAAAAGGGAGATTCTCAAGGCGGAATCGGGGTAACGCGTTCGTTGATCTCGCCAAACTACGGCATCTTGAGGACTAGTCTTAGaGGAAAAAAAGACGACGAGGAGACACGGTGAGCATTAGATGAGAAAGAGACGGCGCGGCGCGGTGCGGCGGAGCGAGACGGAAAGAGATCAAATCTGGATATCAGGATTAGGGTGGGTACGTAATCCGCAGGACGACGGGTGGTAGGAACGGTGGATCCGTCGGCAGATCTCATCGCGCGGAGGAACTCTGCGGGTACTTCGCCCGGCCAAATACGACAAGAGCAGCAGCCTAACTCGAGTAGAGCCGCCGAGATGGTTTACGGCTCGTCGCAAGTTGGTGAAATTTAAGAGCGCATTTAAAACTGGTTTGGCGCGATGCCCTGCGCCTCCCTGCAGCAGGTACTGCCGGCGAGAGATACCGCTGGGCTCCGACAGGAGTTTATCGCCCTAACATTCTTGGAAAGCTTCGGATGGATTTCCCTCCTCAACCCATTTCTTCCGGCAGCACCTCAAATCGTCGCTTCTTTCGAGCTCCCTGTGTCGTCCGTTCTTTCCCCTTGCCACGAAAATTCTTCCAACAGACTCGACACCAAATCGTCACGACGATCATCGGTCAAGATACCCGGAGAAACGTCGCGGACGATGTTACCGTCAGATCGGACGCTTTTAGCTCCGCATTGGAGCTTTTTCCTCGACCGTCTCGGAGGAGATTTAGCGGACCGACAACAAATGAAGGTCATCTTTCGCGCGAGAAAGCGTCTCACCTTGATTTCCGTCTCGTTTCTCCGGAAATTGGATATCAACCGGACGAACTGTAATGTGTACGTTAAATTGCCAATTATATATATAATGTAACAGCTGATTATCTCAAGTGTCCTAAAAACCATTATACTCTTAATTTCTGTGAAAAATGGCGAAAATAAAAAAGAAACCGATCTTAATAAAGATATTCTTCCTGATAGATGCCATGACCCACGTGGAAAACTTTTTAGTTTTGTACAGTGGTATTATACGTTATCTTCCGCTGAACGTAAGACGTGCCTATCGCGCAATTTCATCGCGACGTCGTCGTATAGCGATTATGGCTACTCCATTAAAAATGAATTTTATAAAGGCAATCTTTCCAAGCGATCGTTGTAGGAGAAAAAGGCGAAAGCCGGAGCCAAAGGGGATGAGGCCACTACCTTTGGCTGATCCACTTCGAATGATAATCACCTCTAGGAGACTCAATTTCGCCCTGCTCCGCGTCCTTACCCGTTCCTATCTTCGGAAGGTTCAACGCCGCAGCGGACTGCATCTTTCACTCCCTTCGTCACCACCGCCCTATTCCTATCGCCCTCCGCGCGCCTACCGCCCCTATATCCTTCCCTTCCTTCACtCCTAGACTATTCTGAACGACCTCTTCCCCCATTCGCCAACGCTCACTCCTAACTGATTGGAGTACCAATCAATGCGGCATTCAGGCGGCCGTGCTGAAaCTTTAGGAAATTAACTATTCACTCTCTGGAAATGGTTATTTGGAAGGCCGGAAAGGCAGTCGGGACTACGTTACGGAGAATATGAGCGAAAGGATATGGGCAGACGATGACGATAGATAAGACAGTTCAATTTTCAATTAGGCGTGGACGTAGCGCGCGTAGCTCGGCTCCTACCACGATGCTTACCACGCGTCCCATACGTTAGGAGGAATCCGAGTTATCGATTGCACCTACCTCTTCTCGCGGATCTTACCGCTGGGAAATTTCGTTCGCTTAATGAGATTATTGTACTCGCCGGAAAATCCAGAtAAATTATTCTGATAACTGCGCAACGTCGCCTTCTGGTGTTTCGCGAGCCGATAACGAGCATAGTTAACCGCAGAGAAATTATACGGTCCACATGAGAGAATATATCACTTGGCgCGGGGACGGCGGGGGGGGGGgaGGGgAGACGTACGCGCCTCCAGTCGCGCaCAAAGCCACGCGCGGAGAGAAGTATTAtCCCGGTACTCTCTTTCGTTAATGGCATCGTGGTAATCAACAAAGCCGTACGAGGTAATACGAGGTATTTACAGTTACTCCGGCGCAGCAGAGGGCACCGAGCGTCGAGCGGTGGTAGGCGCATGGGCGGATTTATGCGATGTTTACACTCGCGCAAGGCTGCTAATACGGCTTTCCGACAACAACCCCCACGCCACATCGCGACTGCTCGACTGTGCCCCGCTGTTTGAAAGTGAAACTTCTGTTTGTACGCTAGTTCCAGCTAATCCCGAATATTACGCCGGCGAATCCACCGCCGCGCGTCGGGAGAACGGAAGGCGGCAGCCCAACGCTCAGAACTCTCCGCAAGGCAGAACTGGcGCCTTGAGCGAGGCGGAGAAGTTTTCACAAAGGAGAAGCGCTTCGCCAACTGCCGTGCCGAGCAAAATACATGCGCCCTCTACGAACGTTTGTATTAGACAAGAGCCAATCTTAGCTCCAGCTGGTCCATCTTACGAGAAATCTCGTCGGTTTTGATTCGCACGTCTCGAATCGTATAATAAAAACCGTTGGCCCCAACGACAGTTGTTCGCGTTTACGTTGAGCAACGTTCGACGTGTCTAGAAAACTACAATAAACGGGAGTCGAGAAACGAGATCGTTGAGAAAGGTCTGAAAGATTTCAGCCCTACTACGCCGTGCTGGTACTCCAGTGGATATGTATATACATATACGTATATGTATATGCTCACCGAGAATCCGATCTGCAGTCTCAAACAGCTATTTACCGTAACGTCGGTGGATCGTCGGTACTTTTGTAGCCAGAAGGGAAAATGGATCGGCGGAATCGCCGAGAATGCTACTGCAGTATACAAGTCCGCCGCTGTAACTCATTCACCGATAAATTTCGGCGGGCGAAGCATCGATCACAATGTTCTATTTTGCTCATTGGTGTTTTTGCCGGCGGCATTCGATTCAATTCGAGGATGCGGTGAGTCGATATATCTCTCGAGGTGTCATGAGCCGCCCGTAATGGATGGCGGCTGTTCCCTTACCCTTACTCCGAAAGGAGAGGCCGTGCCAAGAACCTACCCGGTACCCGCAACCCTCTGCTTCTGATCCTGATTTTTGTCTGCTGTCTTCTTCTTATTCCACGCATTATTCTCGCTCTTTCCCTCGCACGGCATTCGCAAAGTGGCCAACAAAGTAGTCTACGTATCTCTCCACGCGATATTTCGTAGCACATTTTCACATTTAATCGCGAGACAATCGGAATCCAAAATATTATCCGTGATATTTAATTTACAAACGTCGTTTGCGTTACCAAAAACAAAATATTTTTTGATTAATCAAATAATATCATAAAAAATTCTTTCAATGCATTTTACCTTTGATGACAGAAAAATCGCTGCCACTCGGGGAACGATGGGATGGATTTCGCGGTAGAACAAGCAAACGCGAAAGCACGCGAAAGGAAATGCACGCGCGACTGGTCCGACAAGGATATTCATAACGTGTGAACGATCACGAGCATTCAAGCTTCGGCGAATGCTAAGACGTGCGAAAATGAGGGTTAAGAAAATCTCGAATCGGGTAGATAATCCTATAGCAGTAGTGAAAATACAATAGGCTTTAATTGCCGGACTTAATTCGTAGAAATACGATTTCGATTAAATGTAACGACTCTCGTGTTTCTCAAATAAAAATGGCACTGAATATAATTGATCACTTATTGTCACTTATCAGTTATATATTAATAAAACAAGACGTTAAAAATTTAATAAGAAAAGATGAAAAAAACAcGATGTTAAAAATTtAACCCTAAAATTAAAATTTTTGTTTATATTAATTATTTTTtATTTATTTTATATTTTTGTCACTAAATTTTGCTTGTATTAAATAAAAATATAAATTTTAATAAGGAAATACGATTTAGGCAGTACTTTCCCCTATTAAGAGGAAGTACATATAGTTTCTAAGAATATTTAGAAATATTTATGCTTTGCATTTAAATAAAATAAAAATGGCGAATTTGTTGCGCTAAATTATTAGTTCTTCAAATATTATTGCAAGAATTATAATCAGTATTGTAAAGTAAATTTTACGAGTATTTTAAGAACTGTTTATGATCTAAAAGGTTATAATTTTGTAAAACGGAGGAATAATCGTCCATCTGTAATGATGCATTTACGTCACAAGAGTGACAcTTCCCTTGAAGCTCGACAGTGCTAATACGAGTTCAAGCCTCTACCATTCTCCGAAGTGAGGATCGATAGTCTAGCATCATTGGGCTCCAACGGCTGATGCGGAGGAGTCGAAGATAGAAGGTACAGAAGGGAGAGGATAGGTGGATGGTGGACGGGGACTTTCGCATGCGGAGTACTGTCGGCTTCGGTTCGCCGAAGGATTAGCATGAATGGCCATCGTCTGCGCCAGTCGAGATAGCAACACAAGCTCTCGCCTATTATTGCTCGGAGAAATTAATCGCATCCATCGCGAATTACTCCGCGATTGCTTTTCCGAGTACAACACGTCTCGCGGTCGATGGGGCAGAGACAGCGGGGAAAGTTCGTGTGATAGGCGTCGTAGTAATTATCAAACGGAACAAAAGAGCGGGCTACCTAATTACTACTTCAATTCCCATATTTATTAATTAGCAATTTATATTATAAAAATTAAAATACTATAAATTGTATATCTTTTAATTATTAACTAATGGGAGACGTTAATAAGTTTAAAACGATGACGACATTAGGAAAAATGTTGCTTTTGTAACGTTTCTAATATTCGTCATTTGAGGAACAGTCGTTACCATTCAGTTCGTATCGAGCTGGATTTGATATAATTATGAAATAGCACACAGCACCCGCTTCCCATCAACATCCCTCCTTTTCCCGAGTCCCTCGGTCTCGGAGAAACAGAAGCGTAATTCCACTCCGAATGCAGTCATTATGGAGATTTTATCAAGGCCTGTTGCCAGCTAGCTAGGCTTCAACTTAACTCGCCCATTACGGCGTTTCCTTTGTCTGCGACTTACACATCGTCATGTCCCGCAGTTCCATTCCGAGCCGAGGGGAACACTGATGAAAGAGATCCGACGATTCGCATACGACGGTTCGATTCACAATGCCGCGCATTCACACACGCGAAAGAGCTCGCGAAGGATTCTGGGATGCATCCGAAAAATAATCACGTGGGACACGAGCATCGACAGCTTGCGCTAGCACGCACGATGGAAACCGCCTGCACAGTTGGACGGATCAGCGAGAGGCGTCGTTTTACGAGGCCGATACTTGAAACGTCGTGCTGAAGTATAAAATTCTACGGGTCATGCAACTGCCAGAGTACCGATCACGCGCATACGTGTACGTAATGCGACTTTAATGCAGCATGTATTATTCAACGATACCACCCGCCGTGCGTGTCCGTCCGACAGCTCGTATACGCGTAATTACGAGCATAGTTGTTTATTGCGAATCCGGCAGTCCGCGCAACGCCTTATCCGGCGGTTATAGACGCGCGTCTCTCAACCCTCCTCGCCAAACGATCCCGATACCTATCCGCCAACCCTCTCGTCCCTCATCTCTCGTCTCTCGTCTCTCGCCATCTCGCCTCCTCGCCTCCTCGCCTCCtCGCctCcTCGCCCCGCCTCCTCTCGCGTCGACAACCCGTGAAGAATGATCGTGGAGTTTCCCTGGTTGGACAGTGACAAATAGAGTATATTCTTGCCAGCTCGGAGAAGCGTGTTTTACGCTTTGTTTACCGTTTGTCATGTTATGCAAAGGAAAGGAATTTTTGCCGGTTGCGTATTCGGCGAATCGACCCTTATCCTACGCGCATGATATGCCTGCGGTAGTTTTACATTATCGCTCGGCTGCGGCGCGGCGCGGCGCTACACAACCATTACCACTAACGATTCGGAGCGACTCCAGGAGTAAAGAGAGAAAGGCCGTAGTCGCATTTGCGGTAAATTGCGCCCATTTACTTCGATTCGCAAACGTAATTTCTGAGATtACCTCACGCTAGTGTCCAGCGATGGTAAAAGTAAGCTCTCTGACCTTCCACGTACGATCCAATATACGATAGAACAATGCATTATTCGACGGCGCGAAATTATTCATAAGCGTCACGAATTGTCCGCAGCGAACGTGGAAACGTTAAACGTTCGGATCtAGTTTGACGAATCTAGAATCGAGACAGTCGGAACCGGACCCTCTCGACTCTCGATCCGGGTGTTTCGCGCGAGGCACGTCGCAATGTTCGGAAAATTGAGGCGAGCCGCATAATACGCATATAGTCGCGTAATAATATGCATACAGGTGCATGCGTGACGACGTGACGCAACGTGACGCCCGAGCGCGGGTCGTTAGCCGTACGGtCGTTTCGCTGGCAAGTCACGCCTAATCACCGATGAATACAACAATCGCAGCATGATGGACGATCGGCAAGCAAGCCGTCCTTTATTAGCGTCGGGTACCCGGGGGCGAGGGGCGGGTGGGAGAGCGGGTGATCATATAATTAGATTAGCGCGCGCCCATCGCGCGTCGTCATCATTTTCCGCGGTGATTTTGGACCGCGCGGGAGATCGGTAACCGCCCTGCGCCGCGCCGCGTCGCGTCGCGTTGCGGCACGGCGGTTACtGTTCTCTCGatACtcGTTGGGATTGTGCTGC

CCTTTGATGTCGCAACGCAGGACGGCACAACGTTCtCTTCCGgCgTaCGAAATCATTTtGGgACACcGTCGCGcGCGGcaCGAGaTTtAATTTgCGCCGGCAACGATCgCGC

Sequencing a genome is now cheap & easy.

CTTTTTtGGTCGAGGTAACCCACACGTCGGGGGgTACGGTTGTATACATTTATGGGAGTAGCACGCTGACCCATAAACATTACTTAGTAGCCGTGCGACATAAAAATTGCCACTCGCGTATCTCAGGTGGTAGTAGTACTCTTAAAAAGTGTCAGCGTGAAAGTAGGCCAAATTACTGCATTTCCGTGTACACGTATCGTAACGCCTTCTCGCGATAAAAGAAGAATAAAATTTTCTCCTAAATATATATGATACCGCAAATGGATTTTGAGTTAGAGAGTAAGATGCAACGAATTGAAATTTATATAAATAATATATGATATTTTCTTATAAATCTTACTAAAtATTTTTTTTGTCAATTCTATCAGAATATTTCGAACGCACGTTTATCTGAACTGAGACGGCGGCATCAATTAGAAGTATTGTGAACGCAGTTGAAATGTATATCCATGAGAAACTCATACTAACGCAGAGCAGTGAAAACGAACACTTGACTCAGGTTTAACCGCGACGAAACGGTATCGGATAATGGACGGGACGAAAAGCGAATTCGACGGCTGGTATCGAGTTACGTGAAGATGTACCTCCGTCTTCGTCGTCATTGAAATTGACCGCGATACGCGAGGTTCACGGAGAGAGACAGAGATCGTAtCTTCCATCTTCTAAATTTCCTTGATTTCTCGTTTATACTCGCGGCAGCAGCCATTTACTTGTTCCACTTGGCGTTCTCAATTTTGCGCTTAGGGAAACAATTAAACCCAAGGATCTCGCATTACATTTTTCCTCCCGCATCTCTACATGTAGCGTATTAAAACCCCGTAGATGCCCGTTCATGCAGAACAAACAACACGGAGCAACATTCGTGCTCTATTTATTTAAGACTCTTATCCAAGGGGGATGAGTTGCAGCGCAGCGAAACTGGTCTGGTATTTATAGTATTTGGATTATGGAATAATCCCTTGCATAGCGACGGCGAAAAGGGAAGGAAAGCCAGGAATGCACGGTGTTTACCGATATCCTTCACCTCTTTTACCTCTTCGTATTCCTCTCTCTCGCGTTTGCCAtCTTTTTTTTtCTCTAAGCGATCTCATTGGTACCGCACGAGGAGCTTCAATCTTTTCTCTTATCTGGATTACTCGTACACAGAGAGTTACAATCCGCTATAAATTCCATCTTTTAAACGCGAAATTCATTCAATTTATTTGCGACCTCAATCATTTTCCCGTGAGATCGACCGAAAAATTCTTAATAAGGATTTATTCGTTTAATCGCGAGCTCTGATAATTTCATTACTCATTGTAACATAAGCGCATTGATATGAATGTACGTATACTGCAAAGTATAGAAAAaTAAATCCGCGTTAAAGTTCTAATTTTTGAAACGTTTATTATTGCTCGTAGAAAAGAAATATTTGTATCTGTGAATACATGTGTCGTAATATTTTAAAAATTAATTCTTTACCTTTATCACGAAAGCTAATTATTTACATATTTACAAATAAACAAAAATATTAGCGTACAAAAATAAGTAAGAGAAAAGAAATTAATTTTGTCTTGGACAAAACGTTTCATTAATGTCGTATTATGTAAAAAAAAATTTAATAAAATGTCAAGATTTTATTTTTGCGAAAGAATAAAAACAAGAGCTTAAGTTCATTCTTTTTGATAGATAATACAAGGTTCTATTTTTGATAAAGACTCATTTTTAGAGGGTTTTCTTTTCACATTTTCCGCGGTACGACTACAATGCATCGTCGAAATACAAAACAAAAAGAAAAGAAAAAGAAATCACAGCGCATCTCGTGCAAAAGCTGCGAACAGTCTACCGTCTACAAACATCGTCAGGAAGATTCTTTCATCTGGTCACGGGGGAGACATCGTTCGCGCAGGTATCTCTCACCGTTGCTGTATATTCATACGTATCTTCGACCTAGTCAAACAACGATTCGACCCAGCACAAAGCAGAGCGGGCGTCAGCGTAGCGTGAAAATTTAACCCGCGACTTGGCGGAGATTTGATTTTTTGGCGTCGGTCAAAAGGCAGACGTGCCCTGATATATCCGCGACGTTATTTGATGCACGGCTACCGGTACAATTGGACTGAGGTAAATAAGAAAGCAGCAACGACAAAAGACTTCCTTCTGTGTGGATGTTAGTCGTACCCTTGGGCGTTGTCTGCCGCCGTATCTCCTAAGTTGGTCGGGGAACGCGGAGTAAGGTTGGACGAATCCTAGTAGATAGCACAGCTGTATTAAATAAATAGAGAATCGCCGAGGCTTTCGACGATGTAGGAGGAAAGAACCGTACTCCTTGAAAGTGGAAAAGGTTGAAGTCAAGCTCCCCCCTCCCTCCCCTCTTCTCCCCGAGAGAACGTCGAGAGGAAGCGTCGTGGATGGAGAACAAAAGTCTGCGCGAAGAAATATCAGAAAAGTCGAACTAATATTTGAGATTACCCATCAATGCTTTTATCCGTCGTGTATCTCCGAAGAAAAAAAGATGGGTATAATTTTAAAAATACATTTGTCAGTGTCTGATATGCGCCGATCTTCTTTCCTCCATGCATTAACGCTTCGTGCGGATACGACCGGGCGTAATCTTAAAAAAAaTGATCGCCCATATACTAAACCACTGACAGCACGTACAGTTCGCCGAGAAGTTTAAGACCACTTTCAGCGCGTTAAACTTTTACACTCTAATTAGAAATTAATAAAGACTAATAGCTTTATCGTCGGTCTATTAAATCCCGTGGAGGAGGTGCTGGAAGTTAGGCAGTCGCGAAGGTGGTCGGTCGGTACACTCGGCAATAAAAGTTTTTTtCTTCGTGGGATAACGGGGACTTTAACTGCAAGAAGAAAGAAAAAAGaGGAAAGAGAGAAAATTCGTTGAAAGCGAAAGTCGAACTACGTTATTTATCTGATGGCCGACACTTCGAAGATTGTTGCGCGGTGTGTCGACGCAGCGCGCAGACGCTCACCAGACGTTTTCTTTTCATTTCTTATTTCTCCCGTCGAAAAAGGaGAGAAAAGGAGAGGATCGGGACGAAGTGAGAAAGAGTAGCGATAGAACGTCGGAAAGGGGAGAAAAAAaGAAATCAATGAGCGGAAACGTGATACGATGGGCGAAGGGCCAGGATGGGGGACAGGGTCGGCCGTGCTGTAGTTTCTCTATTTCCTTCATTAACTCTGATTCAACAGCAGCGTAACTTTTAATTGGAAGTGGTGGGGGgTCGAGTCGAGGCTCATTTCCCGGCGGACTTCCCCATACTGGAATTCAAGCCAATCCCCAAGCGTCTTGCGAGAGGTCACTTCATTCGTTTCGATATTCTCAGATTTTACTTTCATAGCCGTGCGATATAGCATTTGAATTGCTTCGAATTAGAAACGGTAGCGCCTATCtGTTTTTTTTTtAGAGATTAAAGAGATTAAAATCTATAGAAACGTAGTTTTTCGACAGATAAATGCTTCTTTACTGATGTCACATATGATACGCAAAGGGAAATAGATTATAATTAATGATTGTACAATATTTAGTGATATCCCTAACAATAATCATCACGAATGAATAAATATGAATGCATAAAGCGATGTACAATGGCACATTAATTTCCTATCCATTATAAATATAATAAATGTATAAATTGTATTAATGACGAGAACGGGAGATGCGCTGATTTAATTTAAATAAAATCACCAGAGCGTCATCTTCCAAAACGGAAAATAACGGAAGCGACAGATGCTTTCGAAAGCGTCCGTGCCTCCGTCCTTTACTCATATCCAGATAAAGGCACACGTGACGCTCGTAAGATACTGCCGGAAATAAGAGCGATAAAAAGCAAGGCGAATAGTTACACGCCGGGCGACGAGGtCCTTCGAAACGCGAAAATGACGAGCCTGCGATAAAGGACCGCCATCGCGAACGGTAACGGGAGTAACCCCGTGGAATTTAGTATGATCCCCCGCGCACGGCACATCgCGATACTATCGGTTTCTCGTTACCCAGATCGGTATATTGTTATTTCAGCAGTTCTCGGACAAGGTTCACGGACATTCGATAGCGCGAAGGGGACCGGAGCTCCTGAAGAATTCAACGTAACTGGCCGATAATCGGCCTCGTAACGGCTACCCATTTTCCGCGGCACGTCTCGGGGCACCATCATCGAAACGACCTTATTTCCATGCATCCACGTTGCACCTGCTATTGCACCGTAGCCGTCACCGAACTATATCGTCGTCGAACTTATTCGATGTACGATAATCCTTACGGAAAGCTTTAAACAGCTACCTGCATGCATACTAAAGATACGTCCTTcGCTTCTGGGATCCACCTTGTATTCGCGAGACATTGCATAATTTGTATTCGGTGTAAATATGCTATCCGCGTGGCGATCGACCACCGCGAACCGCGCGGAAACTCCACAGGCGGTGCTTGaTAACGTTCCGCGAACGCAGATAAGCAAATGCCAACTTATGGCAGCAGACACTcTACGCGACTTAACAAAATCTCGTATAAACCCGAGATTTTACATGGCAGTTTAACCAAAATAAATTAAGAGAGAAAGACGGAAGAAGAGAGACAGACGACGTGCCTTGCGTCGTCGTAAGAATACGTAAAACCGTGAAATGCTCGAAATGAAGCCGTCCGtATTTTTTGCATACGCTTATATCCGAAGGGCCGCTCTCCCCTCGTAATTGCGTGTCCTCGACAAAAATCTCCTTTCCGATTTGCCTCTTAGTGCTCGCGCATTCCGTCCGCGTACGACGCTTGCAAATCGGCTGCATCGCCGGCGTCACCGGCAGAAAAACGGAGAACTTTCACGGCGAGTCTCGCGGAGCCTCGTACGGCAAAACACCGCGACGACGCCTGCCATTTTGGGCTGGCACGGGAACATAGCAGGGGCCAGGATGACGGAGGGGTGGTGATAGGAAACCCCGGGCAAAAAACaGCGCCTGCAGAGATCCGCGACGCGGCTGTTTAATTCGGGACTTCGCCCGGGGGAGGAAAAAAAaGGGCCGAGGCCGCCGGCTCCTCCTCGCGCCGCTCATGCATTATGAATAATGGAATTTAAATATACGCGAAACAGATCCCCGCTGGGCGAATTCGGCTTAGAGAGGTGGAGTGCAGCCTGGTGCCACGGTGTCGTGTTCGCATTGTTAATTGGCCGGCCGTTtCATTTtCCGTTGCGGCGGCGTAACGTGAGAGACAGCGTAACGGGAAGCGAAACGACACGGCGCGTCGAGCGTGCACAGAGAAGTTGAATTATCGAGGGaGAAAAAGGGCGCGAGGGAGGGGCAGGACGGTCAAACACTGAGAAAGCAGGCAACATCCGCGAACCCGTAAATTAATTAGGGCCAACATAATAGCTGGGAATGGCTGGTAGTGGTGGTTCGTCTTCGTGGTCCAGCTTGCGGTTCCCGAGTGCCCTCGCAGTTCGCGCGGTCATGCCTCGGAAACTACACGATACACCTTGCGTCATCCTTACACGAGGATACCTGTTTGCGATCCCCTGAGGTATTAACGAGCGTATAAGCAGACTCAAAGTACGAACACACTGCTAGTTTCGCGTGCATGTTGCTCGTCGCCGTGCAGACGAGTTAGTACTGTCGACAAAGTAGTCGTAAAGTACGTAAGAGTCTTCCTCTAGTAGCGAGGACTAATCTCCCGGTACATTAATCTATGTATATTTTATGATATACACACCTGTATTTATCGAAAGTTTATGTTTATCTCGAAAATTAACGTTAATTTTCGAGGACGCAAAAGCTGTCGCAAGTTTaTTTAATTTTGTTTGtATTTTTTTTTCTTGCTCATTTTTATTTCGAAGTATGCAAATTGAATCTCTTGGTGGCATAACGAGAATTTTCGAAGCTTAAAGGGATCTGCGTTGGCGCGAAGAGAATGGGACTTGTTTTAAACTTTTCTTTCCAACGAACACCGTTTCCTTCATTGTAACGTAAAAATGGTGAGCTTCTGCGGCGCGATTGGTCTCATCTTTCCTCTCGTATCGTTCGTCATTTTGTCCGAGAGCGTGTAGATATTGTACCCGATAGAACGGAGACGGAATCACGCTATACGGTTCCCTCCCAAATGTTCGTTCCTCACGGGCAAAAGTACAAGTAAAAGTAGGAGGGTCCGACTTTATGACCCTACGAGGCACAATAAAGGTTGATGACTACTAAACGTAGAAGAACACGTATACAGTCCGACGTAAAGTTATTTTTCAATTCCGCGGTCCTCCGCCGCAGTCGTTTTGCCTTGACGTGGAAAAGGAAATTTCCCGGGTTCTAGTCGTCCGGTCTTCTTTtCGTTCTACTGACAATACCATAtTTTCGACATAGATCGATCTCCTTTtCTCTTtCTCTCTCTCTCTCTCTCTCTCTCTTGAAAATGAAAGCGCGGAAGAGCCGGATCGTGCAACGCACAATGCGAGGCCGCTTTGTACGAGGTACGAGGAGTCCGTCATGCGCCGTAAAACGCCGGAATATGCAATACTACTTTCGCAGCGACGGGGCTTGCACGAAGTAATATCGTGAAACGTAGAACCGTTCTTTTCATACGGATATGCGGGAGAAGTTGCTCGTCCGCCCTCCGGCGCATACACGTCGCGCGAGAGTATCGTGCATCCGAAACTCTGAGATGAAACTCTTCTGTAGTCGATATTCGTCGCGATAAATAGATAAGTCTCGGATAAGGTAGAGACATCGATAATTCCGTTACGAGAATACTCGAGAATAAGATCCAAGTGAAGTGATCACGCTCCATATGGTTTCAGATTAATCTCCAATCGGCTGACGAAGGAGGATCATCCTTTTACCGGTGGAAAATAGGGTGGATTGGCGGAGCAAGAAGGCTAAACAGAGAATAAACAGGAGCTATAGCCGAACGAGGGAGAAGGTAAGTAGATGCCTCGGAGAACGTGAGCGAAAAAAGGAAACGGGTACGAGAGAAAAAGAAACGGACGGAATAGCGGCTCGGGATTCGCATCCCAAGGAAAACCAAGGCTATACCGGGGTCTTGGATTATTCGAGGCACGACGACGATCTTCGAGTCAGCCGACTGTCTGTACCGTGAAAGTGGCACGTATCGATCGCACGGCTGGATTATCTTCCACTTCGATCTACGACGATTACTTCCGCCATCGTATATCCGGGCTTTGCACTAGCGAGGCTATTTAAAAATCTGCGCTCAGTAACTACTTATGATTTTTCCATCAGAAACGATTGTGGAGAGAAAAGAGGGAAAAAAAGAGATAGACAGCCTCTGGCTCGAAATGCTAATTTCGCAATCGAGAATTAAAATGCACTTCTTTGTATCTAAATTTTCGTAGAATTAAAATAAAATTGAATAAAGCAATGAATAAATTGAATAAACTAAAATATAGCTAAATATTTTTTCCTCTATACAAGGTGAATATAATTATCAAATATTTAAGTATGTAGATTGAATTTAAACAGCCTCGGAAGAGAAAAGAATCGGATAAACGAAATGCTTTTGCCTCTATTTTCAAGCACGTGACGAATAAAATCTAGCAAAGCTTTTCGACACAATATGTCGACGCAAATGTGGTCTATTTTGGCTAATGATTATTACCGGGAGTCCCGGGCACGGTGTGTCCGCGGCGCGATAAATTAGAGCGCGAATCGACTTCCACGGCCGTTGTAGAAGGTACTTTGGCAAACGTTATTTCTTCCTGTCTCGAAGGAAGCGCCACTCGAAAACTTGGAAAAGTTCGGCCAGCTGCACGCACCGCGATCTCGGGTCCGTTCTGGCTCGGTGGCGTGCGCAGGACGGTTGTGAGACGAGAGAAAGAGAGAAAGGAGGATAGAGCGCGAGAGGGAAAGAAGAGGGAACATCCGCGTGCGTGGTTGTATATGGCGTTAATGGCGGCGAGCATAAAGCATCCCCGcGCCTGCACGCTCGACCACGGTCGTTTACAGTGCCACTATTTATGTTGATAACTTCGGAATGGAGTCAGATACACGGTACCGAGTGCCGGCGGTTCGGCGGTGGTCCGGCAGCGGTCTGGTAGCACTTGCAAAGTATGGGAGAAGAAGGGGGATGGTGGATTCGCGGATCTTTTTGGTCGTGCAAGGAAGGCGGGTCGGTTAGGGTAGGTAGAGAGGAGACGAGCCGAGTCGAGAACAAGATTCAAGCGGAAAGTTTTGCGAGTTAATGGTGCGGAGGTAATGCCGCGGCCGCAACAGCAACAGCCATCCCCCTGCTTGTGTGTTCGCTCGCTCGCTCACTtCTTCCGTTCTCTCCTCTTCGCGCTAGTTCTCTCTCTCTCTCTCTCTCTCTCTCTGtCTTTCTTGGAATAGCCGTAGAAAAAaGGAAAAGAAAGAGAGAGAAAGAACGAGACTCCTTTCTCCCCACGAATTCTCTCCTCTCTTTAAGCACACTCTCTCTTCCTGCaccCCCCCCCcTCcTCTCTCTCTCTTTCTCTCTCTATTTTCTTGTCTTCCTCACTTGCATCATCCCTCGTCCATTTCCcTCTGGAGAACGTGCCACGTTCTCACTTCCTCGTTCGTCGCTACTTTTTTTCTTCTTTAGTTGTCTCCTCTCACACCCTCGAGACGGCCCGATCTTTTCCTCGTACAGCTCCTATCACGAACAGTCGCTAACAAGTGCATCGAATGCAAGTTGCCGACAAACTTCTTCCACCGATTTGTGCTTGTTCTCTGTGCATGCGCGGGCATGTATATCTCTATTAGGCGACATCTGCTCTCAGCTTTTTCATAACGAGGTAGCGCGGATTCGCGCTCCGAGAGACTTCACGAAGCACTTCCGATCTCGCTACAGTAGAATGCGTATTGTATTTTCTTGTCTCTCTCATTTACTCTTTTCTATCTTTCGTATCTAGCGTGAATACTCCCATGAGGAATGTAGAAACCAGATTTTGAAACGGCTTCTTCGTATCTAAATTTTCTTAACTATTTGTTCCTCAAAGTCCATTTTGACGTTATAAATTTTTATTTTTTAATGAGAATGTTTTTATGTGGAGAAGAAAAGTACAACTTTTTTCAAAATGCAATTAAATTTATACAAGACTACTAAGATAAAAAAAGATGCAAATAATAATGTTCAACTTACTAACTGCTATATTATTAAGGCCAAGTTAAATTACAGATTTATGATGTTGCAGAATATAATAGAAAAGTTTCAAGAAAAAAaCATTTTAAAGCTTAAAGAGTTTGTTTTCAAACCCATCAAATTTTTTtCTCTGGCAGTGCTGCTGCTCTGCGACGTCATTTTCAAGTCGATCAATGCAAAGTTAACAAAAGTATTTCAACTTTAAAATCATGCAGAAAGTTGGGAAAAATTTGTTTTCAATTTTACTTAAATTTTAGTTACATTTTTTCTAAAAGCTTAAAATCTCCTCTTTAAAATATGTCTTTAAAAATTGAGCTATGATTTTTCTTCACCGAGTTATCGTAATTTGAAATGGCCAATCGACGTTTTCTTTTCATCACTAGGAATAATGCCGGCGGATTTAAGCTTTCAGAAGATTCCAAGCAAAGTTGAAAACAATTTGTTTGAATTCCGCATGATCTCAAAGTTGAAGCTAAAAAAATTTCACAAGACTTTAAAAGACAAAAAGTCAATGTTGCACAATCGACTTGGAAATAACGCCACTTGGAGCAGAGAAGTATTGCCGAGAAAAAGCTTCGACCGGGTTTGAAAGCAGGATCTATAGGCTTCCAAACTTTTTTTTtGAAAAATGGAGCTTTAAGGTTACTTTTCACGAAATTGTATAAATTGTGTTTTGTTGtTTTTATCATCGATTCGAAATGATCCTTTCATTTTGTTCAAGAATGTATCATTAACTTAATGCAATGATATCTTTTAATAATTCAATACTTCTTACTAATTAGATTTAGAAAAGTTTCATGAACAATTTTAGAACGGTTATTGCTAATTATTCGCCAAAAACAGACGCCTTTCTTCGAGGAGTAACAACTCAAGCTGCATTCGCGGTTGACGGCTTTCCGCGGCGCGTCGCGGTAATATGGCTCCTATTTAATTACCCGGTCCTTTGGGAGCTTAAACCAGCCAGAGCTGGAACGGCTGCGCCGTTATTCTATCTAGACTCCTTTGCTTTCTCAAGCAGGCGCGCGATCAAACCTTCTCGCATAAAAGACAATCGCAGCTGGCAGTCGACGACGCGcGGGACAGTCGAATCATGACCCGCTCCTCTCTAGTTACCGCCGTCAGTCTGCTCTACTTCCAGACGCCGCGCGTAATCTATTCGACATTAGTTGCTTGATTGCACCGTAAAATGCGACGGCGACGTGAACGAGAACGACGACGACGACGACGACGACGACAACGACGACGACAATGACAACGACGACGACGACAAGAGTGGGTTCGTCGGTGCTCGATGGCGCCTCCGATTTCAACCGCAGCACGATGCAAGCCCATTACTATCGCCCGGAGCTAAACGGCACCCGGAGCTCGTGCCATTAAGGGAATCTAGGGTCCGATCCACCTCATTGAATTCCGTTcAATTGCGATTATGATAATGCGTGAACGATCGCCGTGGACGTGAGCTACGGaCAACgAGGGTGTTCGTTCTCGGCTCAGAGAAACGCAGCGATAAAATTATCAGTGACAGCTTCATTTTTGTTACATTTGACGTTGAAAAATTTGCGAAAGAAATGTGCATTATTCAACTACATTGACAATTGTAAATCTTACATGACCTTTTATTAATATACATATATGTAAGATATATGTTCCATCTCTAGTCTCGTTCGATAATGAAGAAATTATAGCTGCAACATAGACGCCGGATTATTCGCGGGGGAAACCGTTGATTATTTGGACTCCGTGGGCTCGGGGTCTGGTTTACTTCTTCCCTTATGTCCGGAGATAATGGACACTATTACCTTAACGAGGCCCCAGTCTCTTAGCCGGTAATTGCTTCGAGATATCCGAGAGAGCTCCGGCGTACGTTGCCGCCTGGTGTTGCAGGCAGAGAACCCaGACGGTATTATTGCCGCCGAGGCTACTCGCCCGTTTCATGGTGGTTATTGTTATGGGCCTGCGGCATTAAGATGTACACCGCACTCTCATACGGGACCCACCACCCCATATACGAGCTGCATATATACTTATGCGGGGAGGATTTCATTACGCCGCTTCATAACTGGCGGTCTTCAAAATCGCGATAAATCGGGATTGTCTTCCTTGTTAAATCAGCTCCCGTTCCCCTTCCTCATATCGCTGACGAAGCCAACGAGACGGATTAATCTGCGCGATTGAATGGCCTCTATGACGTAAGCGCACCGTTTACTGGCACGGCTCCTCGTGTTCACGTGAAACGATTTGCGTCGTAAATATTTTATTTTAACATCGCAACATCAAGACAGACGAGGATCGGCTATTGCCTCGTGATCCTAAAAGGGAGATTCTCAAGGCGGAATCGGGGTAACGCGTTCGTTGATCTCGCCAAACTACGGCATCTTGAGGACTAGTCTTAGaGGAAAAAAAGACGACGAGGAGACACGGTGAGCATTAGATGAGAAAGAGACGGCGCGGCGCGGTGCGGCGGAGCGAGACGGAAAGAGATCAAATCTGGATATCAGGATTAGGGTGGGTACGTAATCCGCAGGACGACGGGTGGTAGGAACGGTGGATCCGTCGGCAGATCTCATCGCGCGGAGGAACTCTGCGGGTACTTCGCCCGGCCAAATACGACAAGAGCAGCAGCCTAACTCGAGTAGAGCCGCCGAGATGGTTTACGGCTCGTCGCAAGTTGGTGAAATTTAAGAGCGCATTTAAAACTGGTTTGGCGCGATGCCCTGCGCCTCCCTGCAGCAGGTACTGCCGGCGAGAGATACCGCTGGGCTCCGACAGGAGTTTATCGCCCTAACATTCTTGGAAAGCTTCGGATGGATTTCCCTCCTCAACCCATTTCTTCCGGCAGCACCTCAAATCGTCGCTTCTTTCGAGCTCCCTGTGTCGTCCGTTCTTTCCCCTTGCCACGAAAATTCTTCCAACAGACTCGACACCAAATCGTCACGACGATCATCGGTCAAGATACCCGGAGAAACGTCGCGGACGATGTTACCGTCAGATCGGACGCTTTTAGCTCCGCATTGGAGCTTTTTCCTCGACCGTCTCGGAGGAGATTTAGCGGACCGACAACAAATGAAGGTCATCTTTCGCGCGAGAAAGCGTCTCACCTTGATTTCCGTCTCGTTTCTCCGGAAATTGGATATCAACCGGACGAACTGTAATGTGTACGTTAAATTGCCAATTATATATATAATGTAACAGCTGATTATCTCAAGTGTCCTAAAAACCATTATACTCTTAATTTCTGTGAAAAATGGCGAAAATAAAAAAGAAACCGATCTTAATAAAGATATTCTTCCTGATAGATGCCATGACCCACGTGGAAAACTTTTTAGTTTTGTACAGTGGTATTATACGTTATCTTCCGCTGAACGTAAGACGTGCCTATCGCGCAATTTCATCGCGACGTCGTCGTATAGCGATTATGGCTACTCCATTAAAAATGAATTTTATAAAGGCAATCTTTCCAAGCGATCGTTGTAGGAGAAAAAGGCGAAAGCCGGAGCCAAAGGGGATGAGGCCACTACCTTTGGCTGATCCACTTCGAATGATAATCACCTCTAGGAGACTCAATTTCGCCCTGCTCCGCGTCCTTACCCGTTCCTATCTTCGGAAGGTTCAACGCCGCAGCGGACTGCATCTTTCACTCCCTTCGTCACCACCGCCCTATTCCTATCGCCCTCCGCGCGCCTACCGCCCCTATATCCTTCCCTTCCTTCACtCCTAGACTATTCTGAACGACCTCTTCCCCCATTCGCCAACGCTCACTCCTAACTGATTGGAGTACCAATCAATGCGGCATTCAGGCGGCCGTGCTGAAaCTTTAGGAAATTAACTATTCACTCTCTGGAAATGGTTATTTGGAAGGCCGGAAAGGCAGTCGGGACTACGTTACGGAGAATATGAGCGAAAGGATATGGGCAGACGATGACGATAGATAAGACAGTTCAATTTTCAATTAGGCGTGGACGTAGCGCGCGTAGCTCGGCTCCTACCACGATGCTTACCACGCGTCCCATACGTTAGGAGGAATCCGAGTTATCGATTGCACCTACCTCTTCTCGCGGATCTTACCGCTGGGAAATTTCGTTCGCTTAATGAGATTATTGTACTCGCCGGAAAATCCAGAtAAATTATTCTGATAACTGCGCAACGTCGCCTTCTGGTGTTTCGCGAGCCGATAACGAGCATAGTTAACCGCAGAGAAATTATACGGTCCACATGAGAGAATATATCACTTGGCgCGGGGACGGCGGGGGGGGGGgaGGGgAGACGTACGCGCCTCCAGTCGCGCaCAAAGCCACGCGCGGAGAGAAGTATTAtCCCGGTACTCTCTTTCGTTAATGGCATCGTGGTAATCAACAAAGCCGTACGAGGTAATACGAGGTATTTACAGTTACTCCGGCGCAGCAGAGGGCACCGAGCGTCGAGCGGTGGTAGGCGCATGGGCGGATTTATGCGATGTTTACACTCGCGCAAGGCTGCTAATACGGCTTTCCGACAACAACCCCCACGCCACATCGCGACTGCTCGACTGTGCCCCGCTGTTTGAAAGTGAAACTTCTGTTTGTACGCTAGTTCCAGCTAATCCCGAATATTACGCCGGCGAATCCACCGCCGCGCGTCGGGAGAACGGAAGGCGGCAGCCCAACGCTCAGAACTCTCCGCAAGGCAGAACTGGcGCCTTGAGCGAGGCGGAGAAGTTTTCACAAAGGAGAAGCGCTTCGCCAACTGCCGTGCCGAGCAAAATACATGCGCCCTCTACGAACGTTTGTATTAGACAAGAGCCAATCTTAGCTCCAGCTGGTCCATCTTACGAGAAATCTCGTCGGTTTTGATTCGCACGTCTCGAATCGTATAATAAAAACCGTTGGCCCCAACGACAGTTGTTCGCGTTTACGTTGAGCAACGTTCGACGTGTCTAGAAAACTACAATAAACGGGAGTCGAGAAACGAGATCGTTGAGAAAGGTCTGAAAGATTTCAGCCCTACTACGCCGTGCTGGTACTCCAGTGGATATGTATATACATATACGTATATGTATATGCTCACCGAGAATCCGATCTGCAGTCTCAAACAGCTATTTACCGTAACGTCGGTGGATCGTCGGTACTTTTGTAGCCAGAAGGGAAAATGGATCGGCGGAATCGCCGAGAATGCTACTGCAGTATACAAGTCCGCCGCTGTAACTCATTCACCGATAAATTTCGGCGGGCGAAGCATCGATCACAATGTTCTATTTTGCTCATTGGTGTTTTTGCCGGCGGCATTCGATTCAATTCGAGGATGCGGTGAGTCGATATATCTCTCGAGGTGTCATGAGCCGCCCGTAATGGATGGCGGCTGTTCCCTTACCCTTACTCCGAAAGGAGAGGCCGTGCCAAGAACCTACCCGGTACCCGCAACCCTCTGCTTCTGATCCTGATTTTTGTCTGCTGTCTTCTTCTTATTCCACGCATTATTCTCGCTCTTTCCCTCGCACGGCATTCGCAAAGTGGCCAACAAAGTAGTCTACGTATCTCTCCACGCGATATTTCGTAGCACATTTTCACATTTAATCGCGAGACAATCGGAATCCAAAATATTATCCGTGATATTTAATTTACAAACGTCGTTTGCGTTACCAAAAACAAAATATTTTTTGATTAATCAAATAATATCATAAAAAATTCTTTCAATGCATTTTACCTTTGATGACAGAAAAATCGCTGCCACTCGGGGAACGATGGGATGGATTTCGCGGTAGAACAAGCAAACGCGAAAGCACGCGAAAGGAAATGCACGCGCGACTGGTCCGACAAGGATATTCATAACGTGTGAACGATCACGAGCATTCAAGCTTCGGCGAATGCTAAGACGTGCGAAAATGAGGGTTAAGAAAATCTCGAATCGGGTAGATAATCCTATAGCAGTAGTGAAAATACAATAGGCTTTAATTGCCGGACTTAATTCGTAGAAATACGATTTCGATTAAATGTAACGACTCTCGTGTTTCTCAAATAAAAATGGCACTGAATATAATTGATCACTTATTGTCACTTATCAGTTATATATTAATAAAACAAGACGTTAAAAATTTAATAAGAAAAGATGAAAAAAACAcGATGTTAAAAATTtAACCCTAAAATTAAAATTTTTGTTTATATTAATTATTTTTtATTTATTTTATATTTTTGTCACTAAATTTTGCTTGTATTAAATAAAAATATAAATTTTAATAAGGAAATACGATTTAGGCAGTACTTTCCCCTATTAAGAGGAAGTACATATAGTTTCTAAGAATATTTAGAAATATTTATGCTTTGCATTTAAATAAAATAAAAATGGCGAATTTGTTGCGCTAAATTATTAGTTCTTCAAATATTATTGCAAGAATTATAATCAGTATTGTAAAGTAAATTTTACGAGTATTTTAAGAACTGTTTATGATCTAAAAGGTTATAATTTTGTAAAACGGAGGAATAATCGTCCATCTGTAATGATGCATTTACGTCACAAGAGTGACAcTTCCCTTGAAGCTCGACAGTGCTAATACGAGTTCAAGCCTCTACCATTCTCCGAAGTGAGGATCGATAGTCTAGCATCATTGGGCTCCAACGGCTGATGCGGAGGAGTCGAAGATAGAAGGTACAGAAGGGAGAGGATAGGTGGATGGTGGACGGGGACTTTCGCATGCGGAGTACTGTCGGCTTCGGTTCGCCGAAGGATTAGCATGAATGGCCATCGTCTGCGCCAGTCGAGATAGCAACACAAGCTCTCGCCTATTATTGCTCGGAGAAATTAATCGCATCCATCGCGAATTACTCCGCGATTGCTTTTCCGAGTACAACACGTCTCGCGGTCGATGGGGCAGAGACAGCGGGGAAAGTTCGTGTGATAGGCGTCGTAGTAATTATCAAACGGAACAAAAGAGCGGGCTACCTAATTACTACTTCAATTCCCATATTTATTAATTAGCAATTTATATTATAAAAATTAAAATACTATAAATTGTATATCTTTTAATTATTAACTAATGGGAGACGTTAATAAGTTTAAAACGATGACGACATTAGGAAAAATGTTGCTTTTGTAACGTTTCTAATATTCGTCATTTGAGGAACAGTCGTTACCATTCAGTTCGTATCGAGCTGGATTTGATATAATTATGAAATAGCACACAGCACCCGCTTCCCATCAACATCCCTCCTTTTCCCGAGTCCCTCGGTCTCGGAGAAACAGAAGCGTAATTCCACTCCGAATGCAGTCATTATGGAGATTTTATCAAGGCCTGTTGCCAGCTAGCTAGGCTTCAACTTAACTCGCCCATTACGGCGTTTCCTTTGTCTGCGACTTACACATCGTCATGTCCCGCAGTTCCATTCCGAGCCGAGGGGAACACTGATGAAAGAGATCCGACGATTCGCATACGACGGTTCGATTCACAATGCCGCGCATTCACACACGCGAAAGAGCTCGCGAAGGATTCTGGGATGCATCCGAAAAATAATCACGTGGGACACGAGCATCGACAGCTTGCGCTAGCACGCACGATGGAAACCGCCTGCACAGTTGGACGGATCAGCGAGAGGCGTCGTTTTACGAGGCCGATACTTGAAACGTCGTGCTGAAGTATAAAATTCTACGGGTCATGCAACTGCCAGAGTACCGATCACGCGCATACGTGTACGTAATGCGACTTTAATGCAGCATGTATTATTCAACGATACCACCCGCCGTGCGTGTCCGTCCGACAGCTCGTATACGCGTAATTACGAGCATAGTTGTTTATTGCGAATCCGGCAGTCCGCGCAACGCCTTATCCGGCGGTTATAGACGCGCGTCTCTCAACCCTCCTCGCCAAACGATCCCGATACCTATCCGCCAACCCTCTCGTCCCTCATCTCTCGTCTCTCGTCTCTCGCCATCTCGCCTCCTCGCCTCCTCGCCTCCtCGCctCcTCGCCCCGCCTCCTCTCGCGTCGACAACCCGTGAAGAATGATCGTGGAGTTTCCCTGGTTGGACAGTGACAAATAGAGTATATTCTTGCCAGCTCGGAGAAGCGTGTTTTACGCTTTGTTTACCGTTTGTCATGTTATGCAAAGGAAAGGAATTTTTGCCGGTTGCGTATTCGGCGAATCGACCCTTATCCTACGCGCATGATATGCCTGCGGTAGTTTTACATTATCGCTCGGCTGCGGCGCGGCGCGGCGCTACACAACCATTACCACTAACGATTCGGAGCGACTCCAGGAGTAAAGAGAGAAAGGCCGTAGTCGCATTTGCGGTAAATTGCGCCCATTTACTTCGATTCGCAAACGTAATTTCTGAGATtACCTCACGCTAGTGTCCAGCGATGGTAAAAGTAAGCTCTCTGACCTTCCACGTACGATCCAATATACGATAGAACAATGCATTATTCGACGGCGCGAAATTATTCATAAGCGTCACGAATTGTCCGCAGCGAACGTGGAAACGTTAAACGTTCGGATCtAGTTTGACGAATCTAGAATCGAGACAGTCGGAACCGGACCCTCTCGACTCTCGATCCGGGTGTTTCGCGCGAGGCACGTCGCAATGTTCGGAAAATTGAGGCGAGCCGCATAATACGCATATAGTCGCGTAATAATATGCATACAGGTGCATGCGTGACGACGTGACGCAACGTGACGCCCGAGCGCGGGTCGTTAGCCGTACGGtCGTTTCGCTGGCAAGTCACGCCTAATCACCGATGAATACAACAATCGCAGCATGATGGACGATCGGCAAGCAAGCCGTCCTTTATTAGCGTCGGGTACCCGGGGGCGAGGGGCGGGTGGGAGAGCGGGTGATCATATAATTAGATTAGCGCGCGCCCATCGCGCGTCGTCATCATTTTCCGCGGTGATTTTGGACCGCGCGGGAGATCGGTAACCGCCCTGCGCCGCGCCGCGTCGCGTCGCGTTGCGGCACGGCGGTTACtGTTCTCTCGatACtcGTTGGGATTGTGCTGC

CCTTTGATGTCGCAACGCAGGACGGCACAACGTTCtCTTCCGgCgTaCGAAATCATTTtGGgACACcGTCGCGcGCGGcaCGAGaTTtAATTTgCGCCGGCAACGATCgCGC

But making ituseable is hard.

Sequencing a genome is now cheap & easy.

Gene predictionDozens of software algorithms: dozens of predictions

Yand

ell &

Enc

e 20

13 N

RG

GTCTACAATGCGATTGTAAAATAGCACGAgAGGTGCATATGATGAACGACTATGTTCCACAACCACAGCTCATATATAACATGATTTtGTTTGCCGAATTCATACACGCATTACAACACACATTGAATTCAATAATAATATCAAATTCACATTCAAAGCTTTCAAGTTAGACAAAAGTTTTAATGCCGTTTTtACCTGTTTTtGAAAAGGTAATTTTCTTTAGATATATACAGTTTGTAATaTTAGGTATTTTATAAACAGTGTGTATATTTCTTACAATATAAAAGACACAATTGCAAACTAGCATGATTGTAAACAATTGCTAAACGGATCAATATAAATTAAAATTGTAATATTAAGTATCAAACCGATAATTTTTATTTATTGTTCATTGTTTGTTCTTTATTTTGTTATTTGTAAATAATGAAA

Evidence


Yand

ell &

Enc

e 20

13 N

RG


Evidence

Evidence


Yand

ell &

Enc

e 20

13 N

RG


Evidence

Evidence

Consensus:


20% failure rate:

Yand

ell &

Enc

e 20

13 N

RG


Evidence

Evidence

Consensus:


20% failure rate: •missing pieces

Yand

ell &

Enc

e 20

13 N

RG


Evidence

Evidence

Consensus:


20% failure rate: •missing pieces•extra pieces

Yand

ell &

Enc

e 20

13 N

RG


Evidence

Evidence

Consensus:


20% failure rate: •missing pieces•extra pieces•incorrect merging

Yand

ell &

Enc

e 20

13 N

RG


Evidence

Evidence

Consensus:


20% failure rate: •missing pieces•extra pieces•incorrect merging•incorrect splitting Ya

ndel

l & E

nce

2013

NR

G


Evidence

Evidence

Consensus:


20% failure rate: •missing pieces•extra pieces•incorrect merging•incorrect splitting

Visual inspection... and manual fixing required.

Yand

ell &

Enc

e 20

13 N

RG


Evidence

Evidence

Consensus:




1 gene = 20 minutes to 3 days

Yand

ell &

Enc

e 20

13 N

RG


Evidence

Evidence

Consensus:





15,000 genes * 20 species = impossible. Ya

ndel

l & E

nce

2013

NR

G


Evidence

Evidence

Consensus:

Monica Dragan https://github.com/monicadragan/GeneValidator

https://github.com/monicadragan/GeneValidator

https://github.com/monicadragan/GeneValidator





15,000 genes * 20 species = impossible. Ya

ndel

l & E

nce

2013

NR

G


Evidence

Evidence

Consensus:

http://afra.sbcs.qmul.ac.ukAnurag Priyam

Crowd-sourcing the visual inspection + correction.

http://afra.sbcs.qmul.ac.uk


Begin

EĞĞĚƐ�ĐƵƌĂƟŽŶ

�ƌĞĂƚĞ�ŝŶŝƟĂů�ƚĂƐŬƐ

Being curated

Curate

Being curated

Curate

Being curated

Curate

Submit Submit Submit

�ƵƚŽͲĐŚĞĐŬ

�ŽŶĞ

/ŶĐŽŶƐŝƐƚ

ĞŶƚ͗�ĐƌĞĂ

ƚĞ�

“ƌĞǀŝĞǁ͟

�ƚĂƐŬ�

�ŽŶƐŝƐƚĞŶƚ͗�create nexƚ�ƌĞƋƵŝƌĞĚ�ƚĂƐŬ





Begin

EĞĞĚƐ�ĐƵƌĂƟŽŶ

�ƌĞĂƚĞ�ŝŶŝƟĂů�ƚĂƐŬƐ

Being curated

Curate

Being curated

Curate

Being curated

Curate

Submit Submit Submit

�ƵƚŽͲĐŚĞĐŬ

�ŽŶĞ

/ŶĐŽŶƐŝƐƚ

ĞŶƚ͗�ĐƌĞĂ

ƚĞ�

“ƌĞǀŝĞǁ͟

�ƚĂƐŬ�

�ŽŶƐŝƐƚĞŶƚ͗�create nexƚ�ƌĞƋƵŝƌĞĚ�ƚĂƐŬ



Scientific software + Facebook + Points + Badges + Redundancy



My aim is to make bioinformatics software:


Shared


Shared

Robust


Shared

Robust

User-friendly

With the fellowship I will:


• Organize a local Software-Carpentry-type event.



• Include software & reproducibility best practices on two new MSc & existing BSc.




• Promote/lobby in line with SSI’s mission (internally/talks/conf//networking/publications/web).




• Promote/lobby in line with SSI’s mission (internally/talks/conf//networking/publications/web).

• Seek funds to create great sustainable bioinformatics software.

Thanks!

[email protected]@yannick__

http://yannick.poulet.org








2013 11-13-SoftwareSustainabilityInstitute@Manchester

Education

Transcript of 2013 11-13-SoftwareSustainabilityInstitute@Manchester