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The Extra Expert in your lab:

UV Detector L-2400

UV-Visible Detector L-2420Diode Array Detector L-2450

Outstanding Performance in HPLC Detection

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14

12

10

8

6

4

2

0

0 2 4 6 8 10 12 1614 18 20 22 24 26 28 30

70

60

50

40

30

20

10

0

0 0.2 0.4 0.6 0.8 1.0 1.2 1.61.4 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4

TC = 0.05 s

TC = 2.00 s

350

300

250

200

150

100

50

0

200 210 220 230 240 250 260 280270 290 300

Ratio = 2.75

Perfect UV-Visible Detection for all your needs

New Standards in HPLCDetection: The L-2400 UVand L-2420 UV-VisibleDetectors

Both detector types are designed for highest sen-

sitivity by utilising the double beam photometric

detector principle in combination with a grating

monochromator. To improve light intensity and

detection sensitivity in the visible range, the

L-2420 UV-Visible detector contains a tungsten

lamp in addition to the deuterium lamp. Detection

wavelength is time-programmable for optimum

detection sensitivity at the absorbance maximum

of each analyte. If negative peaks should be

recorded and integrated, e.g. applying indirect UV

detection for ion chromatography, the polarity

of the detector signal can be reversed. In

stopped-flow mode spectra of the analytes

can be recorded and background-subtracted for

proper substance identification and selection of

the optimum detection wavelength. Both detec-

tors can be operated either by integration into

the LaChrom Elite system with control throughthe EZChrom Elite chromatography data

system or as stand-alone modules via the

optional keypad. In stand-alone mode, several con-

trol functions using contact signals are available.

Highest Sensitivityand Resolution

Advanced optics and innovative electronics com-

bined in the LaChrom Elite UV-Visible detectors

ensure minimised RI effects, a large linear

dynamic range and superior signal to noise

even with the fastest response times. So lowest

trace level detection limits and better quantitative

reproducibility are achieved. The low baseline

drift makes signal detection insensitive to varia-

tions in ambient conditions and leads to optimum

peak integration.A small optical bandwidth ensures

high spectral resolution of the detectors.

Fit for Fast Chromatographyand Micro Applications

The active noise filter combined with a wide

range of response times, enables optimum

peak data acquisition even in high-speed chro-

matography, e.g. using Chromolith columns.

Narrow, rapidly eluting peaks can be recorded

with the highest signal to noise ratio whilst main-

taining peak shape. For all application ranges, from

micro up to semi-preparative chromatography,

different optional pre-aligned and easy-to-install

flow cells are available.

Extreme spectral resolution of the L-2450 Diode Array Detector

for precise compound identification

mAU

Wavelength (mn)

Using the 1 mm slit a maximum resolution

of the benezene spectrum is achieved

mV

min

Equipping the detectors with a micro flow cell

enables highest sensitivity in trace level micro HPLC

mAU

min

Conditions: L-2400 UV detector equipped

with a micro flow cell, L-2100 SMASH pump

Column: Purospher STAR RP-18e 3 m

150-1 mm

Sample: 5 PAH compoundsEluent: Methanol/water 80/20

Flow rate: 50 l/min

Injection volume: 0.5 l

Wavelength: 254 nm

EZChrom Elite UV detector

setup screen

High-speed chromatography using a Chromolith column at 5 ml/min:

Accurate recording of peak shapes if a time constant of 50 ms is selected

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SpecificationsUV Detector L-2400

UV-Vis Detector L-2420

Principle Double beam photometric

detector with grating mono-

chromator

Light source L-2400: D2 lamp

L-2420: D2 lamp + W lamp

Both: Hg lamp for wavelength

calibration

Wavelength range L-2400: 190-600 nm

L-2420: 190-900 nm

Noise 0.3 x 10-5AU

L-2400: at 250 nm

L-2420: at 250 nm and 600 nm

Drift 1.0 x 10-4AU/h

typical 0.4 x 10-4AU/h

L-2400: at 250 nm

L-2420: at 250 nm and 600 nm

Slit width 6 nm

Response time constant 0.05, 0.1, 0.5, 1, 2, 4, 8 s

Wavelength time program yes

Spectrum scan function with background subtraction

yes

Polarity change yes

GLP functions 1. Automatic wavelength

calibration with different

D2 lamp and Hg lamp lines

2. Maintenance logbook: D2,

W and Hg lamp exchange date,

lamp energy, total lamp-on

time, no. of lamp-on switch-

ings

Additionally with

EZChrom Elite:

- Documentation of instrument

serial no. and configuration

- Comprehensive audit trails

and logbooks

Safety Leak sensor, Error handling

Input/Output contact signals - Auto Zero IN

- Lamp Off IN

- START IN

- BUSY OUT

- ERROR IN/OUT

Power requirements 24 V DC, 100 W

Dimensions 340 (W) x 400 (D) x 150 (H) mm

Diode Array Detector L-2450

Single beam photometric

detector with grating mono-

chromator and 1024 bit diode

array

D2 + W lamp

Hg lamp for wavelength

calibration

190-900 nm

0.75 x 10-5AU

at 250 nm and 600 nm

0.5 x 10-3AU/h

at 250 nm and 600 nm

1 nm, 4 nm

0.15 12 s

by EZChrom Elite

by EZChrom Elite

1. Automatic wavelength

calibration with different

D2 lamp and Hg lamp lines

2. Maintenance logbook: D2,

W and Hg lamp exchange date,

lamp energy, total lamp-on

time, no. of lamp-on switch-

ings

Additionally with

EZChrom Elite:

- Documentation of instrument

serial no. and configuration

- Comprehensive audit trails

and logbooks

Leak sensor, Error handling

- START IN

- BUSY OUT

- ERROR IN / OUT

24 V DC, 100 W

340 (W) x 400 (D) x 150 (H) mm

The LaChrom Elite Diode Array Detector is

designed as a single beam photometric detector

with a grating monochromator. It is equipped

with a 1024 bit diode array element to sup-

ply the highest spectral resolution over the

complete wavelength range. For light sources the

detector contains both a deuterium and a

tungsten lamp to ensure maximum sensitivity

even in the visible range. The selectable optical

slit width and electronic diode bunching opti-

mise spectral resolution and sensitivity as

required for each specific analysis. The setting of

the electronic noise filter is coupled to the data

acquisition period. This effectively suppresses

noise whilst at the same time accurately

records peak shapes, particularly fast eluting

peaks in high-speed chromatography.

3-dimensional chromatographic data is recorded

and processed in combination with the EZChrom

Elite chromatography data system.

The following advanced 3D functions

are available:

3D chromatogram data can be background-subtracted and displayed as contour plot,

as 3D chromatograms, as extracted

2D chromatograms and extracted spectra.

Combinations of these display modes can also

be selected.

Automatic peak identification by compari-

son of analyte spectra with library spectra.

Peak purity assessment by comparison of

spectra taken at different positions of the

peak.

Comparison of spectra and calculation of

similarity indices.

Hit list of best fitting spectra with similari-

ty indices.

Max Plot: Chromatogram at absorbance

maximum for each analyte.

Wavelength bunching: Chromatogram

calculated over a specified wavelength range

for optimising sensitivity.

Ratio Plot: Ratio chromatogram calculated

from chromatograms at two different wave-

lengths for assessing peak purity.

Mixed View screen of EZChrom Elite

th diode array detector data

ection of contour plot, spectra,

chromatogram and 3D chromatogram

W lamp

ptics of the L-2450 Diode Array Detector

howing the Hg lamp for automaticwavelength calibration

3D-Chromatography with the Diode Array Detector L-2450Peak Identification and Peak Purity Assessment at Lowest Concentration Levels

Flow cell

Hg lamp

Slit

Grating monochromator

Photodiode array

D2 lamp

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Ordering Information

VWR International GmbH Scientific Instruments

Hilpertstrae 20A D-64295 Darmstadt

Fax: +49-6151-3972-101

www.vwr.com chrom-instruments@de.vwr.com

Designation

L-2400 UV Detector

L-2420 UV-Vis Detector

L-2450 Diode Array Detector

USB Interface board

UI Pad kit for UV or UV/Vis Detector

Power supply: 100 W AC Adaptor

Organiser

(System power supply and solvent tray)

Analog signal output

for UV or UV/Vis Detector

Analog signal output for DAD (2 channels)

e-DIO cable

for use of START/BUSY/ERROR

contact signals in stand-alone mode

System adaptation kits

for micro and semi-micro applications

Cat. No.

890-0402

890-0422

890-0452

890-0702

890-0740

890-0872

890-0852

890-0510

890-0560

890-6145

On request

Designation

Standard flow cell

(10 mm, 13 l)

Semi-micro flow cell

(5 mm, 3.2 l)

Micro flow cell (5 mm, 0.9 l)

Chromolith cell (10 mm, 13 l)

High-pressure semi-micro

flow cell

(5 mm, 3.2 l, 150 bar)

Cuvette holder unit

L-2450 DAD

Cat. No.

890-0550

890-0554

890-0556

use std. cell

890-0555

890-0558

Convenient Maintenance

All maintenance parts are accessible from the

front of the instruments. Therefore, the user can

easily carry out exchange of the detector lamps and

cleaning or exchange of the flow cell.

FDA, GMP, GLP functions included

All detector types are equipped with a mercury lamp for automatic wavelength calibra-

tion. Using the different spectral lines of deuterium and mercury lamps, wavelength calibration

can be performed over the whole spectral range of the detectors. In contrast to calibration

filters (e.g. Holmium oxide filters), which have to be recalibrated themselves periodically, the

spectral lines of the lamps remain completely constant with time and need not to be recali-

brated. On the other hand, for wavelength and absorbance calibration with standard solutions

according to the European Pharmacopoeia, an optional cuvette holder can be supplied.

The detectors contain a maintenance logbook, which records the exchange date of the

lamps, the lamp energy, the total lamp on time and the number of lamp on switchings.

This facilitates regular maintenance and essential documentation. In combination with the

EZChrom Elite chromatography data system, the complete range of GxP-related

functions becomes available e.g., recording of the instrument serial number, hardware

logbooks and audit trails, automatic documentation of the instrument parameters, etc..

When connected to EZChrom Elite software, full compliance with the FDA rule 21CFR Part

11 is achieved. Moreover, module qualification (OQ) can be substantially facilitated using

the optional Auto-Validation software.

Perfect UV or UV-Visible Detectors for all your needs

LaChrom and LaChrom Elite are registered trademarks of VWR International,EZChrom Elite is a registered trademark of Scientific Software Inc.,

Chromolith and Purospher are registered trademarks of Merck KGaA.All other product or company names are trademarks of their respective owners.

L-2400 UV

L-2420 UV-Vis

Cat. No.

890-0500

890-0504

890-0506

890-0509

890-0505

890-0508

Optional Flow Cells for

Automatic wavelength accuracy

check using different spectral lines

of the deuterium and

mercury lamps

UV Detector L-2400 UV-Visible Detector L-2420 Diode Array Detector L-2450