20 Procedures in Obstetrics and Gynaecology Textbook. Chapter 20. Fetal blood sampling

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8/12/2019 20 Procedures in Obstetrics and Gynaecology Textbook. Chapter 20. Fetal blood sampling http://slidepdf.com/reader/full/20-procedures-in-obstetrics-and-gynaecology-textbook-chapter-20-fetal-blood 1/3  91 Chapter 20 Fetal Blood Sampling (scalp pH) Linda Rogers Indication To confirm/exclude fetal hypoxia in the presence of an abnormal CTG. Risks  Trauma to the fetus  Vertical transmission of HIV (therefore only proceed if mother is known to be HIV negative) Accepted normal values pH > 7.25 normal; repeat every 30 minutes if the abnormal CTG pattern continues pH 7.2  7.25 repeat as indicated (usually every 30 minutes) pH 7.1  7.19 fetal acidosis  expedite delivery pH < 7.1 severe fetal acidosis  deliver immediately Requirements  Mother to be at least 4cm dilated  Conical Speculum/Amnioscope and KY jelly  Ethyl chloride  Sponge-holder  Cotton wool / 4 x 3cm swabs  Petroleum jelly  Heparinised capillary tube  Blood gas machine  Large gauge needle/stylette Method This can be done with the mother in lithotomy, or in the left lateral position with the legs drawn up. Insert the speculum/amnioscope into the vagina, so that the narrow end rests on the fetal scalp (away from any fontanelles). Clean any blood/mucous off the fetal scalp, and spray with ethyl chloride. Dab with petroleum jelly (prevents the fetal blood from flowing away), and make a small nick in the fetal scalp with the needle/stylette. Collect the resulting blood in the heparinised capillary tube, insert into the blood gas machine in order to obtain the pH. Interpret results as above.

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Chapter 20

Fetal Blood Sampling (scalp pH)

Linda Rogers

Indication

To confirm/exclude fetal hypoxia in the presence of an abnormal CTG.

Risks

  Trauma to the fetus

  Vertical transmission of HIV (therefore only proceed if mother is known to be HIV negative)

Accepted normal values

pH > 7.25 normal; repeat every 30 minutes if the abnormal CTG pattern continuespH 7.2 – 7.25 repeat as indicated (usually every 30 minutes)pH 7.1 – 7.19 fetal acidosis – expedite deliverypH < 7.1 severe fetal acidosis – deliver immediately

Requirements

  Mother to be at least 4cm dilated

  Conical Speculum/Amnioscope and KY jelly

  Ethyl chloride

  Sponge-holder

  Cotton wool / 4 x 3cm swabs

  Petroleum jelly

  Heparinised capillary tube

  Blood gas machine

  Large gauge needle/stylette

Method

This can be done with the mother in lithotomy, or in the left lateral position with the legsdrawn up. Insert the speculum/amnioscope into the vagina, so that the narrow end rests on thefetal scalp (away from any fontanelles). Clean any blood/mucous off the fetal scalp, and spraywith ethyl chloride. Dab with petroleum jelly (prevents the fetal blood from flowing away), andmake a small nick in the fetal scalp with the needle/stylette. Collect the resulting blood in theheparinised capillary tube, insert into the blood gas machine in order to obtain the pH.Interpret results as above.

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Figure 3

Figure 4