20 Procedures in Obstetrics and Gynaecology Textbook. Chapter 20. Fetal blood sampling
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Chapter 20
Fetal Blood Sampling (scalp pH)
Linda Rogers
Indication
To confirm/exclude fetal hypoxia in the presence of an abnormal CTG.
Risks
Trauma to the fetus
Vertical transmission of HIV (therefore only proceed if mother is known to be HIV negative)
Accepted normal values
pH > 7.25 normal; repeat every 30 minutes if the abnormal CTG pattern continuespH 7.2 – 7.25 repeat as indicated (usually every 30 minutes)pH 7.1 – 7.19 fetal acidosis – expedite deliverypH < 7.1 severe fetal acidosis – deliver immediately
Requirements
Mother to be at least 4cm dilated
Conical Speculum/Amnioscope and KY jelly
Ethyl chloride
Sponge-holder
Cotton wool / 4 x 3cm swabs
Petroleum jelly
Heparinised capillary tube
Blood gas machine
Large gauge needle/stylette
Method
This can be done with the mother in lithotomy, or in the left lateral position with the legsdrawn up. Insert the speculum/amnioscope into the vagina, so that the narrow end rests on thefetal scalp (away from any fontanelles). Clean any blood/mucous off the fetal scalp, and spraywith ethyl chloride. Dab with petroleum jelly (prevents the fetal blood from flowing away), andmake a small nick in the fetal scalp with the needle/stylette. Collect the resulting blood in theheparinised capillary tube, insert into the blood gas machine in order to obtain the pH.Interpret results as above.
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Figure 3
Figure 4