2 4 6 8 10010 1 RLU x10 4 - NBBS [µM] Fig. 1S human GR no hormone Glucocorticoid 10 -11 M Fig. 1S...
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no hormone Thyroid hormone M B RLU x10 4 NBBS [µM] human TRβ Fig. 2S
Transcript of 2 4 6 8 10010 1 RLU x10 4 - NBBS [µM] Fig. 1S human GR no hormone Glucocorticoid 10 -11 M Fig. 1S...
![Page 1: 2 4 6 8 10010 1 RLU x10 4 - NBBS [µM] Fig. 1S human GR no hormone Glucocorticoid 10 -11 M Fig. 1S NBBS does not effect the transactivation mediated by.](https://reader038.fdocuments.us/reader038/viewer/2022100514/5a4d1b4d7f8b9ab0599a625d/html5/thumbnails/1.jpg)
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100 10 1
RLU
x10
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- NBBS [µM]
Fig. 1S
human GR
no hormoneGlucocorticoid 10-11 M
Fig. 1S NBBS does not effect the transactivation mediated by the human GRα.
CV1 cells lacking endogenous steroid receptors or TR were tested for the nuclear receptor transactivation assays as described in figure 4. Increasing concentrations of NBBS were tested for inhibtion of glucocorticoid- (dexamethason) mediated transactivation by transiently transfected cells with the hGRalpha expression vector.
![Page 2: 2 4 6 8 10010 1 RLU x10 4 - NBBS [µM] Fig. 1S human GR no hormone Glucocorticoid 10 -11 M Fig. 1S NBBS does not effect the transactivation mediated by.](https://reader038.fdocuments.us/reader038/viewer/2022100514/5a4d1b4d7f8b9ab0599a625d/html5/thumbnails/2.jpg)
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- 100 10 1
no hormone
Thyroid hormone 10-6 M
NBBS [µM]
human TRβ
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RLU
x10
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Fig. 2S
![Page 3: 2 4 6 8 10010 1 RLU x10 4 - NBBS [µM] Fig. 1S human GR no hormone Glucocorticoid 10 -11 M Fig. 1S NBBS does not effect the transactivation mediated by.](https://reader038.fdocuments.us/reader038/viewer/2022100514/5a4d1b4d7f8b9ab0599a625d/html5/thumbnails/3.jpg)
- 100 10 1
no hormone
Thyroid hormone 10-7 M B
RLU
x10
4
NBBS [µM]
human TRβ
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Fig. 2S
![Page 4: 2 4 6 8 10010 1 RLU x10 4 - NBBS [µM] Fig. 1S human GR no hormone Glucocorticoid 10 -11 M Fig. 1S NBBS does not effect the transactivation mediated by.](https://reader038.fdocuments.us/reader038/viewer/2022100514/5a4d1b4d7f8b9ab0599a625d/html5/thumbnails/4.jpg)
- 100 10 1
no hormone
Thyroid hormone 10-8 M
C
RLU
x10
4
NBBS [µM]
human TRβ
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Fig. 2S
Fig. 2S NBBS does not effect the transactivation mediated by the human TRβ.
CV1 cells lacking endogenous steroid receptors or TR were tested for the nuclear receptor transactivation assays as described in figure 4. Increasing concentrations of NBBS were tested for inhibtion of indicated or thyroid hormone- (A-C) mediated transactivation by transiently transfected cells with the human TRbeta expression vector.
![Page 5: 2 4 6 8 10010 1 RLU x10 4 - NBBS [µM] Fig. 1S human GR no hormone Glucocorticoid 10 -11 M Fig. 1S NBBS does not effect the transactivation mediated by.](https://reader038.fdocuments.us/reader038/viewer/2022100514/5a4d1b4d7f8b9ab0599a625d/html5/thumbnails/5.jpg)
RLU
x10
3
NBBS [µM]
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Fig. 3S
Fig. 3S NBBS inhibits the transactivation mediated by the human progesterone receptor A-form
CV1 cells lacking endogenous steroid receptors were tested for the nuclear receptor transactivation assays as described in figure 4. The human PR-A lacks sequences from the N-terminus of the B-form due to an internal translation start site.
human PR A-form
- 100
no hormoneProgesterone 10-9 M
![Page 6: 2 4 6 8 10010 1 RLU x10 4 - NBBS [µM] Fig. 1S human GR no hormone Glucocorticoid 10 -11 M Fig. 1S NBBS does not effect the transactivation mediated by.](https://reader038.fdocuments.us/reader038/viewer/2022100514/5a4d1b4d7f8b9ab0599a625d/html5/thumbnails/6.jpg)
Fig. 4S
WB: -AR
3 days
7 days
Contro
l
10-5 M N
BBS
10-4 M N
BBS
Contro
l
10-5 M N
BBS
10-4 M N
BBS
Coomassie staining
Fig. 4S NBBS does not influence AR stability. LNCaP cells were treated with 100 µM NBBS for three or seven days. Western blot was performed with anti-AR antibody. Coomassie staining was employed for equal protein loading.
