1st project
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Transcript of 1st project
Medicinal Properties And Insecticidal Properties Of (Wild Watermelon) Citrullus colocynths (Linn.) Schrad.
IT is genarally found in farms as a weed means there is no use of this climber.
Their different part perform different medicinal properties as follow
1. Seed 2. Fruit and Fruit Pulp3. Leaves4. Root
SEED
The Seeds are rich in fatty acids such as myristic, palmitic, stearic, oleic, linoleic and linolenic acids.
Citrullus oil has a good potential use as cooking oil.
The Investigation revealed that seed extract have an insulin tropic effect which show anti diabetic activities.
FRUIT AND FRUIT PULP
The fruit is pungent, a cooling purgative, antihelmetic, antipyretic and carminative.
The fruit pulp is purgative, diuretic and is used against gonorrhea.
A fruit of Citrullus colocynthis is traditionally used for the treatment of diabetes, microbial diseases, ulcer, inflammation, jaundice and urinary diseases in Asian and African countries.
LEAVES The leaves exhibit anti-inflammatory
activity and are diuretic.
ROOT
INSECTICIDAL PROPERTIES
Cucurbitacin E Glycoside Isolated from Citrullus colocynthis show insecticidal effect against Aphis craccivora.
Aphis craccivora can spread viruses. After NMR and IR spectrophotometer analysis
we confirmed that the compound which isolated from Citrullus colocynthis and has insecticidal effect against Aphis craccivora was 2-O-β-D-glucopyranosylcucurbitacin E. (Torkey.H.M and Abou-Yousef,H.M.,2009)
ANTIMICROBIAL ACTIVITY
METHODS - In this procedure we can use either 1st
or 2nd methods to examine antimicrobial activity
AGAR- WELL DIFFUSION METHOD. DISC DIFFUSION METHOD.
AGAR- WELL DIFFUSION METHOD
Petriplates -- 20ml medium -- 24hr culture of bacterial strains.
Wells -- 20 µl of the plant extracts added.
Incubated at 37°C for 24 hours. Measure diameter of obtained
antimicrobial activity.
DIAGRAM - AGAR- WELL DIFFUSION METHOD
Bacteria growing on medium.
Zone of inhibition
Plant extract
DISC DIFFUSION METHOD
Prepare agar plates Inoculation of test microorganisms with
spread plate method Filter paper discs -- 6mm in diameter --
soaked with 15µl of the plant extract Place (and press) in agar plate but no closer
than 24 mm from each other, center to center
Incubate -- 37ºC -- 16 to 18 hours. Measure diameter of obtained antimicrobial
activity.
DIAGRAM – DISK DIFFUSION METHOD
Bacteria growing on medium.
Zone of inhibition
Filter paper disk with plant extract.
PROCEDURE
Collection and identification of plant material
Collect fresh climbing plant. Confermed by botany teacher. Expose to air till it completely dried. Preparation of extract Leaf(Citrullus colocynthis) – Dried –
Powdered – extract by soxhlate (aqueous) – concentrated by Rota evaporator – Extract prepared.
Leaf(Citrullus colocynthis) – Dried – Powdered – extract by socslate (Ethanol) – concentrated by rota evaporator – Extract pripared.
Seed = Dried–powdered- extract by socslate (aqueous) – concentrated by rota evaporator – Extract pripared.
Root = Dried–powdered- extract by socslate (aqueous) – concentrated by rota evaporator – Extract pripared.
POWDERED STEM AND LEAF…
Material - 15 Petri plates – Conical flask containing
nutrient agar – 15 suspension tubes containing saline were sterilized for further use.
Bacterial Strains – E.coli Bacillus megaterium Bacillus cereus Pseudomonas aeruginosa Streptococcus mutans
Actual Method – Pour agar (20ml) in each Petri plate Bacterial strains were poured and
spread Make well (about 6mm) in Petri plate at
center Fill the well with respective extract. Finally incubate the plates for 24 - 48
hrs
OBSERVATIONS After incubation we get a zone of
inhibition around these well. The zone of inhibition is as follow Leaves -
Bacterial Strains
Zone of inhibition Aqueous Ethanol
E.coli
Bacillus megaterium
Bacillus cereus
Pseudomonas aeruginosa
Streptococcus mutans
OBSERVATIONS Seed and Root –
E.coli
Bacillus megaterium
Bacillus cereus
Pseudomonas aeruginosa
Streptococcus mutans
Bacterial Strains
Zone of inhibition
Seed Root
RESULT –
The bitter water melon (Citrullus colocynthis) has antimicrobial (bacteria) activity.
CONCLUSION - There is a great promise for development of
novel drugs from the plant Citrullus colocynthis for dreadful human diseases.
They affects the growth of insect and degrade them so it can use in several insecticide, pesticide etc.
They have some medicinal properties i.e antidiabetic activity,
They shows the antibacterial activity which helps to inhibit the growth of bacteria which affects the plants.
REFERENCES
THANK YOU