A Student's Grammar of the English Language (Sidney Greenbaum, Randolph 1 Quirk, 1990)
1Managed by UT-Battelle for the Department of Energy Presentation_name Molecular Photovoltaics and...
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1 Managed by UT- Battelle for the Department Presentation_name Molecular Photovoltaics and the Optical Activation of Neural Cells E. Greenbaum 1 , M. S. Humayun 2 , B. R. Evans 1 , T. Kuritz 1 , I. Lee 3 , C. P. Pennisi 4 1 Oak Ridge National Laboratory, 2 University of Southern California, 3 University of Tennessee, 4 Aalborg University March 18-19, 2009 Oak Ridge, Tennessee
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Transcript of 1Managed by UT-Battelle for the Department of Energy Presentation_name Molecular Photovoltaics and...
1 Managed by UT-Battellefor the Department of Energy Presentation_name
Molecular Photovoltaics and the Optical Activation of Neural Cells
E. Greenbaum1, M. S. Humayun2, B. R. Evans1, T. Kuritz1, I. Lee3, C. P. Pennisi4
1Oak Ridge National Laboratory, 2University of Southern California,3University of Tennessee, 4Aalborg University
March 18-19, 2009Oak Ridge, Tennessee
The Artificial Retina
3 Managed by UT-Battellefor the Department of Energy Presentation_name
4
7
PSI on a Heterostructure
S S S S S S SS S SS
Au
PSI PSI
OH
OH
OH
OH
OH
OH
OH
OH
OH
OH
OH
Measurement of Electrostatic Potential in Nanometer-Resolution: Schematic Illustration Kelvin Probe Method in Lift Mode
ha
c
b
d
–+ +
0 V
Au
–
+
–
Light
0 nm
0 V
5 V
-5 V0 nm
15 nm
30 nm
Lighton
Lighton
LightOff
LightOff
a b
AFM and KFM Images of PS I Reaction CentersAFM and KFM Images of PS I Reaction Centers Immobilized on GoldImmobilized on Gold
PS I Photovoltage Topography: Summary of Three Types of Measurements
Dark (A) Light (B) (B – A) a Light–Dark b
Periphery 1 (V) 0.77 ± 0.14 -0.47 ± 0.26 -1.24 ± 0.29 -1.13 ± 0.14
Center (V) 0.62 ± 0.08 -0.41 ± 0.24 -1.03 ± 0.25 -0.97 ± 0.04
Periphery 2 (V) 0.99 ± 0.20 -0.59 ± 0.26 -1.58 ± 0.32 -1.20 ± 0.19
No. of PS I Averaged 12 22 4
a Calculated difference between columns B and A. b On the same PS I.
The vertical arrows in above figure indicate three selected voltage points we used in this table: the two extremities and the center.
Conceptual Illustration of Idea
WERI-RbI retinoblastoma cellsWERI-RbI retinoblastoma cellsfused with (proteo)liposomes
Add (proteo) liposomes
WERI-RbI retinoblastoma cellsfused with (proteo)liposomes and loaded with fura-3
Load with the fura-3 dyeIlluminate with 600-700 nm
Spectral Properties of System
FIRST STEP: PSI RECONSTITUTION INTO PROTEOLIPOSOMES
RECONSTITUTION
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Light triggers increase in cytosolic Ca2+ in proteoliposome-treated cells
Kinetic Time Course Experiments in the Presence and Absence of PSI and External Calcium
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Spatial Distribution of the Electric Potential From Photosystem I Reaction Centers in Lipid Vesicles
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Distribution of the Electric Potential Close to a PSI Reaction Center Inserted in the Vesicle
MembraneThe Role of Ionic Strength
0 0.1 M
IEEE Transactions on Nanobioscience, Vol. 7, No. 2, June 2008, Pennisi, Greenbaum & Yoshida
