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Chapter-9 Result and Discussion

9.1 RESULT OF DIFFERENT GROUP TEST

Chemical

constituents

Test

Nymphoides

hydrophylla

Test fo al!aloi"s -Mayers test

-Wagners test

-Hagers test

#

Test foca$oh%"ates

-Molischs Test

-Benedicts Test

&

Test fo e"ucin'

su'a

Fehlings Test &

Test fo ca"iac

'l%cosi"es

Legals test &

Test fo (a)onoi"s Lead acetate Test &

Test fo sa*onins Saponin test &

Test fo *h%tosteols Libermann-Burchard test &

Test fo te*ens -Salo!sis Test

-"opper #cetate Test

&

Test fo *henols Ferric "hloride Test &

Test fo *oteins $anthoproteic Test #

Test fo tannins %otassium dichromate Test &

Test fo 'ums &um test #

9.2 CYTOTOXIC STUDY

9'( )esult o* di+erent group

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In our current study all crude extracts showed positive result indicating that test samples are

biologically active. Each of the test samples showed different mortality rates at different

concentrations. Plotting of log of concentration versus percent mortality for all test samples

showed an approximate linear correlation. From the graphs, the median lethal concentration

(L!", the concentration at which !"# mortality of brine shrimp nauplii occurred$ was

determined for the samples. %here was no mortality in the negative control groups indicating

the test as a valid one and the results obtained are only due to the activity of the test agents.

9.2.1 Cytotoxic activity of the test samples ofNymphoides hydrophylla

&rine 'hrimp &ioassay is the simplest process which in most cases indicates potential

cytotoxic and antitumor properties. &y using this process, developed by )eyer, we

determined L!"of crude methanol extract and the results are given %able *.+

Table 9.1: ffect of C!"#e $etha%ol xt!act ofNymphoides hydrophllao% &!i%e Sh!imp

'a"plii

Co%

(C)

(*+,

-o+ C

$o!tality

-C/0(*+,ml)

&ase# o%

lo+C

+inc%stine Sul*hate

Co% (C)

(*+,ml)

-o+ C

$o!tal

-C/0

(*+,

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Chapter-9 Result and Discussion

ml) ity ml)C$ C$

00 .-""!***+ +""

+-.-

/" +.-""- +""

".0*

200 .0"+" *" " +.0"+"0 *"

100 " +" + *"

/0 +.-**1""/ 1" ! ".-**1 "

2/ +.0*1*/"""* -" .! ".0*1*/ 1"

12./ +."*-*+""+0 /" +.! "."*-*+ 1"

.2/ ".1*!""+1 /" ".-! "."/+ !"

3.12/ "./*/!"" " ".0+! ".!"!+! 0"

1./2

/

".+*0""- +" ".+!-! "."-+ "

0.451

2/

".+"1"**1 "" "."1+! +.+"1+ +"

C$rude )ethanol Extract2

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-('5 -( -'5 '5 ( ('5 .

.

/

,

0

(

(.

*123 4 /'.2 6 5.'50

)7 4 '95

Fig9.1: Effect of Vincristine ulphate on !rine hrimp Nauplii

-( ( .

.

/

,

0

(

(.

*123 4 .'., ln123 6 ,'5,

)7 4 '95

*123 4 /'.2 6 (0'5,)7 4 '95

, -otalit%

8 Mortality

Logarithmic 18

Mortality3

Linear 18 Mortality3

Lo' C

, of motalit%

Fig 9.": Effect of Crude #ethanol E$tract of %ea&es on Nymphoides hydrophylla.

Table 6: 7es"lts of the test sample ofNymphoides hydrophlla

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Chapter-9 Result and Discussion

Sample -C/0 (*+,ml) 7e+!essio% 8"atio% 7 2

3incristine

'ulphate (positive

ontrol$

".0* y 4 0/."x 5 !.! 67 4 ".*!

rude methanol

extract (Leaves$

+-.- y 4 0/."!x 5 +.!!1 674".*!+

%he L!"values of crude methanol extract of Nymphoides hydrophylla leaves found to be

+-.- 8g9ml (%able :2 Figure !.+,and !.$. %he positive control vincristine sulphate showed

L!"at a concentration of ".0*8g9ml.

From the results of the brine shrimp lethality bioassay it can be well predicted that the crude

methanol extract of leaves ofNymphoides hydrophylla have cytotoxic property.

Table 5.1: Th!ombolytic activity of Nymphoides Hydrophyllawith9''( )esultThom$ol%tic acti)it% of ymphoides

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M:F ( M:F . M:F M:F/ M:F 55

(

(5

.

.5

5

/

/5

5

Chat Title

"oncentration 8 o* lysis

Fig 9' 8 o* clot lysis in di+erent concentration o* methanolic *raction

9.3.2 o! bla% a%# St!eptoi%ase

9.'Discussion of Thom$ol%tic acti)it% of

!actio% Co%ce%t

!atio%

(m+,ml)

;1 ;2 ;3 Of

Clot

-ysis

$ea% in' acti)it% ofAllamanda nerifolia

T!eatme%t Co%ce%t!atio% Optical #e%sity

of samples i%

hypoto%ic sol"tio%

($ea% < SD)

i%hibitio% of

hemolysis

Co%t!ol +.!- >> 1.0+

%he extracts of Nymphoides Hydrophylla, also sub>ected to analy?e for membrane

stabili?ing activities using standard protocol and the ac@uired results were expressed in

statistically.Aere, crude methanol extract ()E$ of different concentration showed different

effect with a value of various inhibition of hemolysis caused by hypotonic solution. Bmong

them, it shows maximum # inhibition of hemolysis (/!./+#$ at -mg9ml concentration. Chere

Bcetyl salicylic acid (".+" mg9mL$ revealed 1.0+# inhibition of hemolysis induced by

hypotonic solution correspondingly.

6esults represented in means ; standard deviation (n 4 0$2 Level of 'ignificance ===p D

".""+, ==pD "."+, percent inhibition of migration was calculated relative to acetyl salicylic

acid.

9.= )esult o*-em$ane sta$ili>in' acti)it% of

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