1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla...
-
Upload
dora-stokes -
Category
Documents
-
view
218 -
download
4
Transcript of 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla...
![Page 1: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/1.jpg)
1/53
Andrej Savol May 2013
TECBio 2013 Journal Club Intro
With help from…
Chakra ChennubhotlaJason CastroAlpay TemizArmaghan NaikGustavo RohdeIvet BaharGrant SchauerJudith Klein-Seetharaman
![Page 2: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/2.jpg)
2/53
Outline
What is Journal Club?
Paper Selection and Comprehension
Making good slides
Presentation skills
Example Presentation: Worobey 2010
1
3
2
![Page 3: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/3.jpg)
3/53
Outline
What is Journal Club?
Paper Selection and Comprehension
Making good slides
Presentation skills
Example Presentation: Worobey 2010
1
3
2
![Page 4: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/4.jpg)
4/53
What is Journal Club?
Learning the current state of knowledge in a given field.
![Page 5: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/5.jpg)
5/53
Learning the current state of knowledge in a given field.
http://matt.might.net/articles/phd-school-in-pictures/
All human knowledge
![Page 6: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/6.jpg)
6/53
Learning the current state of knowledge in a given field.
http://matt.might.net/articles/phd-school-in-pictures/
![Page 7: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/7.jpg)
7/53
Learning the current state of knowledge in a given field.
http://matt.might.net/articles/phd-school-in-pictures/
The outside perspective…
![Page 8: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/8.jpg)
8/53
Learning the current state of knowledge in a given field.
http://matt.might.net/articles/phd-school-in-pictures/
The inside perspective
![Page 9: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/9.jpg)
9/53
Learning the current state of knowledge in a given field.
http://matt.might.net/articles/phd-school-in-pictures/
If you want to push the boundary, you need to know the territory!
![Page 10: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/10.jpg)
10/53
1) Select recent paper from high impact journal (Science, Nature, Cell, PNAS)
2) Understand the paper
3) Prepare slides
4) Review with mentor
5) Give 15 minute presentation (5 min. qns)
Journal Club Assignment
![Page 11: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/11.jpg)
11/53
But first, how does a scientific paper get published?
http://moviesoddity.com/the-best-10-movies-that-didnt-win-an-oscar/
![Page 12: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/12.jpg)
12/53
Paper Selection Resources
http://f1000.com/
Paper Selection and Comprehension
![Page 13: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/13.jpg)
13/53
Read
Be a critical reader:
“On first reading, believe everything, on the second, believe nothing” –Donald S. Coffey
Understand the paper
![Page 14: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/14.jpg)
14/53
Read
What is the main message? (perhaps not title)Ex. Protein A is NOT required for Protein B function.
What question is being answered?Ex. How are proteins A and B related?
What are the methods of study?Ex: Microscopy, Computer Simulation, Literature Search
How was the data analyzed?
Read through first time then answer following questions
Understand the paper
![Page 15: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/15.jpg)
15/53
Read
Read through second time and answer following questions
Why was an experiment/analysis performed?
What is the purpose of every figure?
Do claims in text follow from data? (i.e. good interpretation)
Understand the paper
![Page 16: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/16.jpg)
16/53
ReadSome useful criteria…
Evaluate the paper
Good Data?
Goo
d In
terp
reta
tion?
Yes
Yes
No
No
![Page 17: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/17.jpg)
17/53
ReadSome useful criteria…
Evaluate the paer
Good Data?
Goo
d In
terp
reta
tion?
Yes
Yes
No
No
[i.e., particles traveling faster than light]
![Page 18: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/18.jpg)
18/53
ReadSome useful criteria…
Evaluate the paper
Good Data?
Goo
d In
terp
reta
tion?
Yes
Yes
No
No
[i.e., particles traveling faster than light]
![Page 19: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/19.jpg)
19/53
ReadSome useful criteria…
Paper Selection and Comprehension
Good Data?
Goo
d In
terp
reta
tion?
Yes
Yes
No
No
[i.e., bacteria with arsenic replaced DNA backbones]
![Page 20: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/20.jpg)
20/53
ReadSome useful criteria…
Evaluate the paper
Good Data?
Goo
d In
terp
reta
tion?
Yes
Yes
No
No
[i.e., bacteria with arsenic replaced DNA backbones]
![Page 21: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/21.jpg)
21/53
ReadSome useful criteria…
Evaluate the paper
Good Data?
Goo
d In
terp
reta
tion?
Yes
Yes
No
No
[i.e., special relativity]
![Page 22: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/22.jpg)
22/53
ReadSome useful criteria…
Evaluate the paper
Good Data?
Goo
d In
terp
reta
tion?
Yes
Yes
No
No
[i.e., special relativity]
![Page 23: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/23.jpg)
23/53
Read
Have the author’s confused correlation/causation?
Evaluate the paper
![Page 24: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/24.jpg)
24/53
Read
# Dr. Pieter Tans, NOAA/ESRL (www.esrl.noaa.gov/gmd/ccgg/trends/) and Dr. Ralph Keeling, Scripps Institution of Oceanography (scrippsco2.ucsd.edu/)
Pieter Tans, NOAA/ESRL (www.esrl.noaa.gov/gmd/ccgg/trends/) and Ralph Keeling, Scripps Institution of Oceanography (scrippsco2.ucsd.edu/)
Evaluate the paper (example)
![Page 25: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/25.jpg)
25/53
Coin flip example..
Criminal example…
Null Hypothesis
![Page 26: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/26.jpg)
26/53I. Good, “When batterer becomes murderer,” nature.com, Jan. 1996.
abuse
murder
Null Hypothsis
Coin flip example..
Criminal example…
![Page 27: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/27.jpg)
27/53I. Good, “When batterer becomes murderer,” nature.com, Jan. 1996.
Null Hypothsis
abuse
murder
![Page 28: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/28.jpg)
28/53I. Good, “When batterer becomes murderer,” nature.com, Jan. 1996.
Null Hypothsis
abuse
murder
![Page 29: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/29.jpg)
29/53
Section Summary:
Read until you can confidently argue
for and against results in the paper! 1
![Page 30: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/30.jpg)
30/53
Outline
What is Journal Club?
Paper Selection and Comprehension
Making good slides
Presentation skills
Example Presentation: Worobey 2010
1
3
2
![Page 31: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/31.jpg)
31/53
• Every slide has a title• Units and axis labels are clear on figure• 7 and 7 rule (guide)• Use consistent fonts/color scheme• Include references• Insert equations with LaTex(it) (www.latex-project.org)
rather than y = x/z
Make Slides
![Page 32: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/32.jpg)
32/53
slide title slide title slide title
Making Slides – Layering Content
![Page 33: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/33.jpg)
33/53
Practice your talk at least twice
Then…
…out loud……without notes……standing up……in front of others, if possible.
![Page 34: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/34.jpg)
34/53
Present
No notes! – use your slides as guides
Have ~3 points to say on each slidefigures and/or text will help guide you
Minimize reading lots of text
Make eye contact
Repeat asked questions
Answer honestly - if you don’t know, ok to say so
Be careful with the laser-pointer!
![Page 35: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/35.jpg)
35/53
What’s wrong with the following slides?
![Page 36: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/36.jpg)
36/53
• Expression of Hsp28 is both constitutive and heat-shock- induced expression with a large maternal load
• Hsp27 is found mostly in the cytoplasm but is has also been seen in nucleus and associated with the plasma membrane and the nuclear membranes as well as in the nucleus itself.
• Hsp27 exists in various oligomerization and phosphorylation states. It is thought to form higher order structures of very large size – often larger than 700 kDa, though these might be aggregates instead of ordered structures. Phosphorylation plays a role in determining the higher order structure of Hsp27
• Multiple functions have been ascribed to Hsp27 proteins
- Hsp27 can act as a chaperone like many other heat shock proteins- Hsp27 can have an effect on Redox homeostasis through the transferrin
receptor- Hsp2 7 was thought to be an actin capping protein ,but is not thought to be an
actin monomer sequestering protein which has the effect of depolymerizing actin.
- Inhibitor of induced cell death in culture- Correlation with survival of tumors
![Page 37: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/37.jpg)
37/53
• Expression - constitutive and heat-shock-induced
• Localization - cytoplasm, nucleus, membranes
• Various oligomerization and phosphorylation states
• Multiple functions…
- Chaperone- Redox homeostasis- Actin depolymerizing- Inhibitor of cell death in culture
What is known about Hsp27
![Page 38: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/38.jpg)
38/53
wild-typeGMR-rpr/+ ; TM6B/+
mal
es
fem
ales
GMR-rpr/+ ; Hsp27F6/+
eyes
![Page 39: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/39.jpg)
39/53
wild-type reaper expression
mal
esfe
mal
es
Reaper expression minus Hsp27
Hsp27 mutant suppresses reaper eye phenotype
![Page 40: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/40.jpg)
40/53
Results
![Page 41: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/41.jpg)
41/53
• Sequence• Ramachandran• Solvent access• Cryo-EM• Homology modeling
What we have shown
![Page 42: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/42.jpg)
42/53
Section Summary:
Practice your presentation and review
slides with mentor/lab/etc.
Engage audience!2
![Page 43: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/43.jpg)
43/53
Outline
What is Journal Club?
Paper Selection and Comprehension
Making good slides
Presentation skills
Example Presentation: Worobey 2010
1
3
2
![Page 44: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/44.jpg)
44/53
How old is simian immunodeficiency virus?
or
![Page 45: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/45.jpg)
45/53
Backgroundclosely related to HIV
molecular clock estimates suggest MRCA of ~100s years
SIV is common among nonhuman primates, related viruses are 1000s years old
Solution: find isolated population of SIV positive monkeys
![Page 46: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/46.jpg)
46/53
Background
Solution: find isolated population of SIV positive monkeys
![Page 47: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/47.jpg)
47/53
Background
Solution: find isolated population of SIV positive monkeys
![Page 48: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/48.jpg)
48/53
MethodsCompared gene sequence of SIV pol from positive animals
http://www.hiv.lanl.gov/content/sequence/HIV/MAP/landmark.html
![Page 49: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/49.jpg)
49/53
MethodsCompared gene sequence of SIV pol from positive animals
http://www.hiv.lanl.gov/content/sequence/HIV/MAP/landmark.html
![Page 50: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/50.jpg)
50/53
Conclusions
The genetic diversity observed required at least 10,000-12,000 years
By recalibrating molecular clocks, MRCA for ALL SIV is estimated at 32,000 years ago
Implies humans have had many incidences of contact over history
http://www.hiv.lanl.gov/content/sequence/HIV/MAP/landmark.html
![Page 51: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/51.jpg)
51/53
Criticisms
Only one ORF sequenced in tissue samples
Monkeys could have been reintroduced by humans from mainland
http://www.hiv.lanl.gov/content/sequence/HIV/MAP/landmark.html
![Page 52: 1/53 Andrej Savol May 2013 TECBio 2013 Journal Club Intro With help from… Chakra Chennubhotla Jason Castro Alpay Temiz Armaghan Naik Gustavo Rohde Ivet.](https://reader038.fdocuments.us/reader038/viewer/2022110323/56649d925503460f94a79179/html5/thumbnails/52.jpg)
52/53
Section Summary:
Get big points across!3