15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina...
Transcript of 15. Dr. Patrick Kobina Arthur - University of Ghana...Microsoft PowerPoint - 15. Dr. Patrick Kobina...
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Rustad et al, 200902/23
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The birth of Antibiotics:
Pencillium notatum
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• Pleuromutilin produced by Clitopilus sp(Pleurotus sp )
• Active against gram positive bacteria• Binds to 23S rRNA
PleuromutilinRetapamulin
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HIT-rate (WDF, n=189)>>366X~4
M. smegmatis : 78%S. aureus : 68% E. coli : 44% C. albicans : 31%
HIT-rate (MEF, n=29)>>(+143)light/dark
M. smegmatis : 98% / 78%S. aureus : 58% / 43% E. coli : 20% / 0%C. albicans : 13% / 0%
HIT-rate (ABF, n=111)>>800+
M. smegmatis : 87%S. aureus : 19% E. coli : 8% C. albicans : 30%
HIT-rate (TEF, n=0)>>400+
M. smegmatis : --S. aureus : --E. coli : --C. albicans : --
HIT-rate (SBF, n=0)>> 201
M. smegmatis : --S. aureus : --E. coli : --C. albicans : --
Total: 2700+09/23
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AMP 40 AMX 40 VAN 40 INH10 EMB 20 PZD 40 MOXI 0.5 RIF 10 LIN 5 TET 20 CHLO 40 ERY 40 STREP 20
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Reverse Phase C-18
Size Exclusion LH-20
Bioassay
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BCG cell (GFP)Drug treatment
Quantitative measurements of Cell viability and growth13/23
Mass Spec1D Proton NMR13C, 15N,HSQC, HMBC, HMQC, COSY, TOCSY, ROESY, NOESYetc…14/23
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Miguel A. MatillaGeorge P. C. SalmondDepartment of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QW
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Project Team MembersSamuel Yaw Aboagye (2009-2011)
Priscilla Koduah (2010-2011)
Vincent Amarh (2011-2012)
Clement Opoku-Temeng (2012-2013)
Samuel Mawuli Adadey (2012- )Gloria Baaba Arkaifie (2013- )Arnold Abraham (2013 - )Precious Cramer (2013 - )
Mentorship and Project SupportDr. Paul A. LarteyLaGray ChemicalCompany – NsawamGHANA
Collaborators
University of CambridgeDr. Finian Leeper
Imperial College LondonDr. Brian Robertson
ETH-Zurich Prof. Ruedi AebersoldOlga SchuberMichael Zimmermann
University of Maryland -CPProf. Herman Sintim
UG-NMIMRProf. Dorothy Yeboah-Manu
FundingDAAD-GermanyGrand Challenges-CanadaTWAS-ItalyIFS-SwedenEMBO/SNF-SwissGates Foundation/NMIMRCAPREx/Alborada