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The world leader in serving science

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Date

released 7/01/2012

Thermo ScientificDiagnostic Products

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Unmatched Portfolio of Products and Services

ReagentsAnalyticalInstruments

Consumables SoftwareLaboratoryEquipment

PRODUCTS

SERVICES

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On-siteService

DeliveryFinancialService

The world leader in serving science

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Your Partner in Science

Your Partner in Science

Committed to our customers’ goals for better results and greater productivity

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Products for Diagnostics

Thermo Scientific Passive Adsorption Surfaces:

• PolySorp™

• MediSorp™

• MaxiSorp™

• MultiSorp™

• Immulon 1B

• Universal Bind

• Immulon 2HB

• Immulon 4HBX

• Enhanced Bind

Thermo Scientific Covalent and Affinity Capture Surfaces:

• CovaLink™

• Immobilizer™ Streptavidin

• Immobilizer™ Amino

• Immobilizer™ Glutathione

• Immobilizer™ Nickel-Chelate

• BioBind

• Streptavidin

The broadest surface portfolio on the market

www.thermoscientific.com/diagnosticplates

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Key Capabilities

We are able to modify the polystyrene surface to meet your specific application.

Three different types of Thermo Scientific surfaces: • Passive Adsorption Surfaces • Covalent Surfaces• Affinity Capture Surfaces

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Passive Adsorption Surfaces

Bio-molecules are bound to the surface via several non-covalent bonds.

The collective force can be substantial.

• In general, larger molecules bind better• Most successfully used for proteins (Antibodies)• Moderate success with moderate complex to complex lipids

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Adsorption Principles

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Passive Adsorption Surfaces

Guide to recommended biomolecule to be bound onto the passive adsorption surface.

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Covalent Surfaces

Biomolecules are attached to the surface by strong covalent bonds.• Involves forming a bond between

a functional group such as NH2 on the biomolecule and another functional group incorporated into the surface of the plate

Advantages• Binding is not size dependent• Several biomolecules that do not

bind well on passive surfaces can be immobilized - peptides, nucleic acids, highly charged proteins

• Better molecular orientation produces higher signals and greater assay sensitivity

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Covalent Surfaces

Spontaneously reactive electrophilic group

Peptide

Optimal distance ensures minimal steric hindrance

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Covalent Surfaces

Immobilizer Amino• Covalent attachment of peptides,

proteins and antibodies(pH dependent)

CovaLink• Binds to COOH (carboxy)

and PO4 (phosphate) groups

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Affinity Capture Surfaces

Plate surface is pre-coated with a specific capture molecule:

CAPTURE• Streptavidin• Nickel-Chelate• Glutathione

LIGAND• Biotin• Poly-histidine• Glutathione-S-Transferase

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Affinity Capture Surfaces

Thermo Scientific Nunc Immobilizer Streptavidin• Attachment of biotinylated

oligonucleotides/peptides/proteins etc.

Thermo Scientific Nunc Immobilizer Glutathione

• Attachment of Glutahione-S-Transferase-tagged fusion proteins

Thermo Scientific Nunc Immobilizer Ni-Chelate

• Attachment of Histidine-tagged fusion proteins

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Guide to the Right Surface

Most common biomolecules:• Antibodies (glycoproteins) - passively adsorption plates

• MaxiSorp, Enhanced binding or Immulon 4HBX

• Antigens and proteins – passively adsorption plates• MediSorp, Immulon 2B, Microlite 2+, Microflour 2+

• Lipids (hydrophobic) – passively adsorption plates• Polysorp, Immulon 1B, Universal binding

• Peptides and small molecules - covalent attachment• CovaLink or Amino Immobilizer

• Biotinylated biomolecules• Streptavidin Immobilizer

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Diagnostic bottles

Thermo Scientific Nalgene Bottles, Carboys and Vials

It’s easy to take for granted the containers you entrust with your valuable samples and solutions. But not all bottles are created equal. Nalgene is the #1 choice, with more than 3,000,000 safely at work in laboratories around the world.

• Leakproof design• Small bottles are perfect for diagnostic

reagents• Size range from 0.5 ml through 20 ml• Multiple materials available – durable,

break resistant• Multiple packaging options to meet

your requirements

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Tools

Solid Phase Guide• Interactive, online version:

http://thermo.dirxion.com/immunoassayguide• Printed

Immunoassay Plate Guide

• Interactive, online version:http://thermo.dirxion.com/immunoassaybrochure

• Printed

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Tools

Thermo Scientific Antibody Sample Kit

• F96 MaxiSorp, Medisorp, Immulon 4HBX, Immulon 2HBX, Enhanced Binding

Thermo Scientific Peptide Sample Kit

• F8 Amino Immobilizer and F8 CovaLink