1 RIKILT P.O. Box 123, 6700 AB Wageningen Contact: [email protected]
1
On-site detection of food allergens EU-Marie Curie IAPP: IMPR oved food safety monitoring through E nhanced imaging NanoplaS monicS . IMPRESS (2011-2015) Willem Haasnoot 1 , Ana Frangolho 2 , Gerardo Marchesini 2 , Andrea Valsesia 2 and Claus Nielsen 3 We would like to thank the European Commission (EU-Marie Curie IAPP programme) and the Dutch Ministry of Economic affairs, Agriculture and Innovation for their financial supports. 1 RIKILT P.O. Box 123, 6700 AB Wageningen Contact: [email protected] T + 31 (0)317 48 50 79 www.wageningenur.nl/RIKILT 2 Plasmore SRL, Via G Deledda, Ranco VA, Italy 3 Schafer-N APS,Kystvei 6, Humlebaek, 3050 Denmark The exchange of knowledge results in the identification of monoclonal-specific epitopes on food proteins. Biosensor chips with epitope-related synthetic peptides give high stability (reusable) and improved sensitivity. An array of allergenic protein-related peptides with a mixture of the specific monoclonal antibodies in a label-free transportable biosensor will have high (commercial) value for the on-site detection of food allergens. Figure 1. (A) Absolute responses obtained in the Biacore after the immobilization of k-casein (mw 19000 Da) and the epitope-related peptide (mw 1246 Da) and after the binding of the monoclonal antibody (Mab), showing the higher responses and better stability (during 75 cycles of Mab injection and regeneration) with the immobilized peptide. (B) An improved sensitivity is observed by the application of the immobilized peptide in combination with a lower amount of Mab. 1 Monoclonal antibodies against (total) protein Peanut Hazeln ut Egg Milk Soy 2 3 Imaging Nanoplasmonics biosensor High density peptide array of (allergenic) proteins 2 mln 15-mer peptid es Array of synthetic peptides Cow milk protein (k-casein) detection using a synthetic peptide Multiplex immunoassay in food Synthetic peptides Antibodies + protein sequences for epitope mapping Improved sensitivity Improved response and stability A B Partners + expertise Exchange of knowledge Results of the first example Conclusions Acknowledgement Prototype “Impressor” Optical Label- free November 2012, RIKILT Symposium
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On-site detection of food allergens EU-Marie Curie IAPP: IMPR oved food safety monitoring through E nhanced imaging Nanopla S monic S . IMPRESS (2011-2015). 1 RIKILT P.O. Box 123, 6700 AB Wageningen Contact: [email protected] T + 31 (0)317 48 50 79 www.wageningenur.nl/RIKILT. - PowerPoint PPT Presentation
Transcript of 1 RIKILT P.O. Box 123, 6700 AB Wageningen Contact: [email protected]
On-site detection of food allergensEU-Marie Curie IAPP: IMPRoved food safety monitoring through Enhanced imaging NanoplaSmonicS. IMPRESS (2011-2015)
Willem Haasnoot1, Ana Frangolho2, Gerardo Marchesini2, Andrea Valsesia2 and Claus Nielsen3
We would like to thank the European Commission (EU-Marie Curie IAPP programme) and the Dutch Ministry of Economic affairs, Agriculture and Innovation for their financial supports.
1 RIKILTP.O. Box 123, 6700 AB WageningenContact: [email protected] + 31 (0)317 48 50 79www.wageningenur.nl/RIKILT
2 Plasmore SRL, Via G Deledda, Ranco VA, Italy
3 Schafer-N APS,Kystvei 6, Humlebaek, 3050 Denmark
The exchange of knowledge results in the identification of monoclonal-specific epitopes on food proteins. Biosensor chips with epitope-related synthetic peptides give high stability (reusable) and improved sensitivity. An array of allergenic protein-related peptides with a mixture of the specific monoclonal antibodies in a label-free transportable biosensor will have high (commercial) value for the on-site detection of food allergens.
Figure 1. (A) Absolute responses obtained in the Biacore after the immobilization of k-casein (mw 19000 Da) and the epitope-related peptide (mw 1246 Da) and after the binding of the monoclonal antibody (Mab), showing the higher responses and better stability (during 75 cycles of Mab injection and regeneration) with the immobilized peptide. (B) An improved sensitivity is observed by the application of the immobilized peptide in combination with a lower amount of Mab.
1
Monoclonal antibodies against (total) protein
PeanutHazelnutEgg
MilkSoy
2 3
Imaging Nanoplasmonics biosensor
High density peptide array of (allergenic) proteins
2 mln15-mer
peptides
Array of synthetic peptides
Cow milk protein (k-casein) detection using a synthetic peptide
Multiplex immunoassay in food
Synthetic peptides
Antibodies + protein sequences for epitope mapping
Improved sensitivityImproved response and stabilityA B
Partners +expertise
Exchange ofknowledge
Resultsof the firstexample
Conclusions
Acknowledgement
Prototype “Impressor”
Optical Label-free
November 2012, RIKILT Symposium
