1 Lab Safety

8/13/2019 1 Lab Safety

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By

Dr. Alaa Eldin Salah Eldinssistant Professor of Cellular and Molecular Biology

Majmaa UniversityPreparatory Year ProgramColleges of Medicine and Dentistry1 st SEMESTER 1431/1432 H

Course Title: Human BiologyFor Premedical Student

1- Lab Safety andrules


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Why is Lab Safety Important?

Lab safety is a major aspect of every lab basedscience class.Lab safety rules and symbols are needed so that

students do not injure themselves or theirclassmates.


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Lab Safety Rules

1. Always follow the teachers directions and onlydo lab work when a teacher is present.

2. Conduct yourself in a responsible manner at alltimes. No Horseplay!

3. Do not touch any equipment, chemicals, or othermaterials until told to do so.

4. Do not eat food, drink beverages, or chew gum inthe lab. Do not use lab glassware as food orbeverage containers

5. Report ALL accidents to your teacherimmediately, even if you think it is minor.


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Clothing

Wear: Goggles/safety glasses Closed-toed shoes

Clothing made ofnatural based fibersuch as cotton

Tie back long hair

Do NOT Wear: Sandals Jewelry

Loose or Baggyclothing


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Lab Safety Equipment

Safety Shower Safety GogglesEye Wash


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Lab Safety Equipment

FireExtinguisher

Fire BlanketFume Hood


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Lab Safety Symbols

Found in your textbook

Glassware

Heat

Eye and Face

Sharps

Electrical

Animal

Chemical

Fire


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Other Chemical Hazards and Symbols

Flammable

Explosive

Toxic/Poison

Irritant

Corrosive

Environmental


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Chemical Hazard Symbols andDefinitions

Flammable Any substance that will burn

if exposed to an open flame.

Explosive A substance that mayexplode if exposed to heat or flame.


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Toxic/Poison A substance that can

lead to death if inhaled, ingested, orabsorbed by the skin.

Corrosive A substance that candestroy or burn living tissue and caneat away at other materials.



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Irritant - A substance that causesinflammation upon contact with skin ormucous membranes.

Environmental - Substances that are

harmful to the environment. They mustbe disposed of properly, not washeddown the drain.



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Accidents and Injuries

Report ALL accidents and injuries to your teacherimmediately!!!

Be aware of safety hazards associated with each

chemical you use.Eye accident Wash the eye with copious amounts ofwater for at least 15 min.

Fire Burns Stop, Drop, and be covered with a fireblanket or soaked with water. Do not remove clothingstuck to victim. Get medical attention immediately.


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Waste Disposal

First ask your teacher where/how to dispose of

waste.

Never pour anything down the drain unlessyou are told to do so.

A waste bottle will be supplied for substance

that cannot go down the drain


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Reasons for proper waste disposal

Hazardous chemicals down the drain can lead to

pollution of ground water, lakes, rivers, etc.

Plants and animals will die if they are exposed tohazardous chemical waste.

Serious health problems will become present in people if

hazardous waste finds its way into drinking water.


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Laboratory Rules

No eating or drinking in labs

Do not enter stockroom or back halls

Clean up after yourself


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Laboratory Etiquette

Clean your personal workspace after use

Wipe down benches with disinfectant before and after use.

Ensure that no trash is left behind

No trash in the sinks

Push in chairsPlace lab items back on carts and trays


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Learning Center & Open Labs

The LC and open labs are a bonus, not a right, your

flexibility is appreciated

Hours are posted on LC and lab doorsHours are subject to change without notice

Some study items may not be available a day or two

before an exam

Dont wait until the last day to study


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Laboratory Safety

1. Laboratory Hygiene

2. Sharps Safety

3. Safety Equipment

4. Fire Safety

5. Chemical Safety


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1. Laboratory Hygiene

Scrub hands thoroughly when finished Avoid cross contamination

Do not touch self, faucets, doorknobs, notebooks, pensetc. with gloves on.

Keep a pen or two in your drawer for lab use only

Clean and disinfect your workspace


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2. Sharps Safety

Sharps include Razor and scalpel blades Needles and pins

Microscope cover slips Broken glass

Place sharps in sharps boxes

Broken Glass into glass box (ask for help)NO SHARPS OR GLASS IN THE TRASH


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3. Safety Equipment

Do not hesitate to use safety equipment.It is for student use as well as instructoruse.Shower and eyewash (15 min. soak)First aid kit

Fire extinguisher, Fire blanket, Alarm pulls


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4. Fire Safety

Never leave flames unattendedDo not use flammables near ignitionsourcesFire Extinguishers Pull ring, aim at fires base, squeeze handle, sweep

back and forth (evacuate if >1m 3)

Fire BlanketFire alarm pulls


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5. Chemical Safety

Wear gloves and glasses whereappropriateFollow instructors directions Dispose of waste properly- Do Not pourdown the drain


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Laboratory Safety Rules

1. Many things happen in the lab, and you cannever be sure how your area has been used, orwhether it was properly cleaned up.

Because of this, and possible contamination fromthe animals, you should not bring food or drinkinto the lab with you! Also, don't put anything

that's come in contact with the lab table-pens,pencils, fingers, etc. - into your mouth or eyes.


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2. Be on time! There are several ways being latecan hurt your grade directly, but there aresafety considerations as well

Lab instructions, including safety items, are goneover at the beginning of the labs.


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3. It's always a good idea to look your lab exerciseover ahead of time, and make notes of themodifications and warnings given at the

beginning of the class .


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4. Any time you're not sure, ask!!


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5. Wash your hands before and especially afterworking in the lab.


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6. There are drawers and coat hooks available -keep all unnecessary objects out of your andother people's way.


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7. Although labs rarely involve dangerousmaterials, some can have lasting effects onclothing, so you might want to bring a protectivecover-up.

With some procedures, protective eye coveringswill be required. If you have long hair or

dangling jewelry, keep them out of your workingmaterials.


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8. Clean up your stuff! Your area should look at theend of your lab like it did when you started.

When in groups, everyone is responsible for clean-up. Points may be lost if this is not done.


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9. When working with chemicals or cultures, makesure you do not mix things together in thesource glassware - that includes droppers andmixing rods.

It is not always safe to mix tap water into materialsby washing them, either.


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10. Dispose of all waste materials properly. Thewastebasket is near the door.

Broken glass does not go into the wastebasket -there's a special box for it next door in the preproom.

Notify the instructor any time something getsbroken or if you're injured, even mildly.


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11. Try to always stay alert while you're in the lab.

Know where the emergency equipment - first aidkit, fire extinguisher, eyewash fountain, etc -are (most are on the front wall or just acrossthe hall).

Know the appropriate route out of the buildingduring an emergency.


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