1.2.3.4.5.6.

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Chemometric Optimization Of A SIA Promethazine

Hydrochloride Assay

Method

Idris, AM; Assubaie, FN; Sultan, SM

ELSEVIER SCIENCE BV, MICROCHEMICAL JOURNAL; pp: 7-13; Vol: 83

King Fahd University of Petroleum & Minerals

http://www.kfupm.edu.sa

Summary

A sequential injection analysis (SIA) method for the assay of promethazine

hydrochloride, based on its oxidation by acidified cerium(W), was optimized. Three

chemometric approaches were applied: (i) factorial design (3(3) applied to surface

plot and 2(3) applied to effect factor) for screening the potential interacting variables,

(ii) univariant for optimizing insignificantly interacting variables and (iii) simplex for

optimizing potentially interacting variables. The optimum experimental conditions

were 30 mu l of 0.38 mol/l sulphuric acid, 30 mu l of 3.99 x 10(-3) mol/l cerium(IV),

20 mu l of promethazine hydrochloride and 20 mu l/s flow rate. The detection limit

was 7.032 x 10(-5) mol/l and the calibration curve was linear up to 1.563 mol/l with a

correlation coefficient 0.9998, accuracy range of 89.0-101.5%, relative standard

deviation 1.1 % (n = 10) and sample frequency at least 20 samples/h. The method was

applied to tablet form and validated with the British Pharmacopoeia method. The

developed SIA method is fully automated, reproducible, sensitive, rapid and reagent-

saving, and therefore suitable for routine control in tablets form. (c) 2006 Elsevier

B.V All rights reserved.

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For pre-prints please write to: abubakridris@hotmail.com

Copyright: King Fahd University of Petroleum & Minerals;http://www.kfupm.edu.sa