(0)Start system, computer, LSM (AIM) programme, Hg ARC lamp (HOT???)

and tungsten lamp

!!

(1)Start lasers, then find sample using VIS

(2)Set configuration using LSM

(3)Set scan resolution, speed & repetition in Mode

(4)Set pinhole, excitation & detection for each Channel

Single track

Multi trackUse Find to get initial image, Split and Palette to adust

(5)Control Zoom and Crop in Mode and Select

(6)Open database for image storage

(7)Set Z stack with first/last & optical slice

(8)Display 3D using projection

(9)Time Series and Edit Bleaching

(10)Using Lambda stacks, Save to Spectra DB for Linier Unmixing