07 LSSI ppt - Life Sciences Summer Institutematsc.org/Unprotected Docs/Teacher PPTs/07 LSSI...
Transcript of 07 LSSI ppt - Life Sciences Summer Institutematsc.org/Unprotected Docs/Teacher PPTs/07 LSSI...
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LSSI 2007
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•This project was funded through the San Diego Workforce Partnership, Inc. by a grant awarded under the President's High Growth Job Training Initiative, as implemented by the U.S. Department of Labor's Employment and Training Administration
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Amgen Bruce Wallace Biotechnology Lab Program
Lab 8Genomic DNA Extraction from Buccal
Epithelial Cells/PCR Analysis
Lab 7Purification of mFP from Overnight Culture
Lab 6Preparing an Overnight Culture of E. Coli
Lab 5Transforming E. coli with a
Recombinant Plasmid
Lab 4Confirmation of Restriction & LigationUsing Agarose Gel Electrophoresis
Lab 3Ligation of pARA & pKAN-R Restriction Fragments
Producing a Recombinant Plasmid, pARA-R
Lab 2Resitriction Analysis of pARA & pKAN-R
Lab 5aTransformation of E. coli with pARA-R
Lab 4aConfirmation of pARA-R Restriction Digest
Using Agarose Gel Electrophoresis
Lab 2apARA-R Restriction Digest:
An Introduction to Plasmids and Restriction Enzymes
Lab 1An Introduction to Volumetrics & Pipetting
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As all humans, we want to feel that we are a part of a greater mission – Annie Glidden (Biogen idec)
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Teacher Share-out: Taking it Back to the Classroom
1)Teachers shared laboratory activities and simulations for implementation in the classroom.
2)School-to-Career ApplicationA. Industry Guest Speakers (Scientists and
other Industry Personnel)B. Industry Site Visits and ToursC. Internship Opportunities
3)Sharing of available educational resources
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High Schools:Temecula Valley Unified:Brett Baughman Great OakOceanside Unified:Christine Meza El CaminoTuyet-Mai Vo El CaminoVista Unified:Christi Newman VistaSan Marcos Unified:Matthew Leader High Tech North CountyEscondido Union:Nicole Hoofard EscondidoPoway Unified:Vicki Binswanger WestviewLisa Smedley Rancho BernardoMalinda Dixon WestviewSusanne Nelson PowaySan Diego Unified:Ellie Vandiver Crawford Andy Mangahis Patrick HenryLisa Yoneda Mira Mesa
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High Schools (cont.):La Mesa:Paul Messier Helix CharterGrossmont Unified:Glenn Melero El Cajon ValleySteele Canyon:Betty Jensen Steele CanyonSweetwater Union High:Darci Kimball Castle ParkMike Sixtus Bonita VistaRoderick Warner Bonita VistaVictor Rodriguez SouthwestPrivate:Jamie Howard Lutheran
Colleges:San Diego Community College:Anar Brahmbhatt Ph. D. MesaTheresa Bolanos Ph.D. City Sandra Slivka Ph. D. MiramarLinda Woods Ph. D. MiramarSharon Price MiramarSouthwestern Community College:Nouna Bakhiet Ph. D. Southwestern
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RECOMBINANT PIPETTE
pARA
MICROFUGE
E. COLI
pKAN
AGAROSE
MICROLITER
POLYMERASEENZYMELIGASEDNA
TAQ
PCR
PLASMID
RNA
BIOTECHNOLOGYCENTRIFUGE
KARL STRAUSS
CONATUS
PFIZERBIOGEN-IDEC
DOWPHARMA
INVITROGEN
GENENTECH
ESTUDY
LYSIS
SALK
HYDROPHOBICPERCIPITATE
PRIMER
ELUTE
HYDROPHILIC
RESTRICTION
BIOCOM
PROTEINTRANSFORMATION
ALUCENTRAL DOGMAAMINO ACID
AMGEN
PURIFY
DIGEST
ELECTROPHORESIS
BUCCAL
ALIQUOT
GENOMIC
mFPDIGEST
HPLC
SPECTROPHOTOMETER
BamH I
VOLTAGE
SB BUFFER
STICKY END
Hind III
CHO
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California Standards & Amgen Lab Curriculum
1a-d, 1g, 1k-m
1a, 1m1aInvestigation & Experimentation
3cPhysics
2a, 2b, 2h2h, 5g, 8b, 8d,
10a
Chemistry
1b, 1h, 4a, 5a-e
1b, 5a-e1a-d, 4c, 5a-c, 10d
Biology
Lab 1: Lab 2: Lab 2a: Lab 3:
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California Standards & Amgen Lab Curriculum
1a-d, 1f, 1g, 1j, 1k
1a-d, 1j, 1m
1a-c1a-e, 1j, 1i
Investigation & Experimentation
5a, 5c, 5ePhysics
8b2a, 6d, 10b
4d, 10aChemistry
1a, 1b, 5a-d, 10d
1a, 1c, 5c-e, 7a,
8a
1b, 1d, 5c-e
1h, 4c, 5a-e
Biology
Lab 4: Lab 4a: Lab 5: Lab 5a:
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California Standards & Amgen Lab Curriculum
1a, 1b, 1d, 1m
1a, 1m1d, 1mInvestigation & Experimentation
5a, 5c3ePhysics
2a, 5g, 8b, 8c,
10a
3c, 10cChemistry
1a, 1b, 2e, 3a, 5c, 7a,
7b
1b, 1d, 1h, 4a-c, 4e, 5a-c,
10d
5c, 5eBiologyLab 6: Lab 7: Lab 8:
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Biotech Helping Society:• Industrial and
Environmental– Fermented Foods and
Beverages• Medical and
Pharmaceutical– Antibiotics– Vaccines– Monoclonal Antibodies– Hormone Synthesis
• Diagnostic Research– PCR– ELISA– Sequencing
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Good Work Ethic
Detail Oriented
Dependability
Good OrganizationSkills
Good CommunicationSkills
Good Problem-SolvingSkills
Safety Oriented
Ability to get alongwith others
Flexibility
Team Oriented
Fast Learner
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We are the home depot of Biotech. We will not build anything for you, but we will give you everything
you need- Lisa Peterson (Invitrogen)
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www.karlstrauss.comSan Diego, CA
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KARL STRAUSS BREWERY
http://www.karlstrauss.com/
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History
• 1989: Opened first Karl Strauss microbrewery in San Diego
• 1992: Opened main brewery on Santa Fe street
• Currently distributing to 2,500 Southern California locations
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Brewing Process
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Karl Strauss Products
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Employment Opportunities• Master Brewer• Sales Representative• Customer Service• Chemist
• Quality Assurance
• Taste Testers• Distribution
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If you have a good idea, you have to know how to write it well- Steve Kenney (eStudy Site)
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“However long we live, our lives are but a brief moment in the trajectory from the past into the future. Nevertheless, it is long enough to make a difference, for better or for worse, in our lives and in the lives of others, both in our time and beyond.”– Jonas Salk [Mission : fund biomedical research
aimed at understanding diseases…]
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SALK INSTITUTEhttp://www.salk.edu/
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Salk Institutehttp://www.salk.edu/index.php
Sector:• Diagnostic Research Biotechnology• Medical Pharmaceutical Biotechnology• Agricultural BiotechnologyStage of Growth:• Research and Development• Collaborative / International• Master Plan Update 2006• Motto—Where Cures Begin…
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Salk InstituteEmployment
Staff of ~1,200 from 50 countries (59 faculty, 300postdoctoral fellows & 100 graduate students) Current Openings:1. Faculty Positions-Senior Research/Investigator2. Postdoctoral Training-Bioinformatics, Research,
Biophysics, Gene Expression, Neuroscience…3. Staff Positions-Custodial Supervisor, Animal
Technician, Lab Assistant, Research Assistant, Micro-Injectionist, IT technician, Bioinformatics…
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Salk InstituteTimeline
• 1952 – Dr. Jonas Salk discovers the polio vaccine• 1960 – Architect Louis Kahn designs the institute• 1962 – Groundbreaking of Institute (March of Dimes)• Ongoing –
Home to 11 Nobel Laureates – Drs. Frances Crick, Ronato Dulbecco, Roger Guillemin, Sidney BrennarOne of leading research institutes in the world…
• Areas of Research –Neuroscience-nervous system disorders Biology and Genetics-cancer & diabetes Infectious Diseases-AIDS & anthraxPlant Biology-food supply & global warming
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Salk InstituteTechnology
Tools & Equipment: [shared w/other labs]• Light, Fluorescent, & Electron Microscopes• Computers Simulators, Flow Cytometer, etc.• DNA Synthesis, Sequencing, & Thermocyclers (PCR)
Core Technology:• Bioinformatics – comparative genomics & evolutionary• Cytometry & Molecular Imaging (CCMI) – sort cells, etc.• Mass Spectrometry/Proteomics – protein identification• Microarray/qPCR – gene expression profiling• Transgenic – transgenic & knock-out mice, IVF, embryo
cryopreservation & rederivation
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Each member of a row team practices what seems a repetitive task, but it moves them toward a glorious goal. –Sandy Slivka (Miramar College)
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Genentechhttp://www.gene.com
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Genentech VisionUtilize the science of
biotechnology to become a leader in revolutionizing the
treatment of patients with cancer,
immunological diseases and
angiogenic disorders.
www.gene.com
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Oceanside Facility
Manufacturing facility for Monoclonal Antibody-based pharmaceuticals; primarily Avastin®.– In Phase III trials– 1 month -> 18 kg -> $70,000,000.– “Smart Bomb” approach to angiogenesis
inhibition.
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Working at Genentech
Employment Opportunities– Operations– Facilities– Manufacturing– QC/QA– Accounting– HR– Environmental Awareness (Sustainability)
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Post-Secondary Education:– Educational reimbursement up to $10K/yr
Undergraduate Opportunities:– Summer Internship
programDesired Skills:
– Detail Oriented– Safety Oriented– Good Work Ethic– Technical Skills – Record Keeping– Flexibility
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I used to make nail polish and lipstick. Although I made a great contribution to society, I find this more
rewarding- Mike Greig (Pfizer)
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Who are they?• “The Home Depot of the Life Science Industry”- Lisa Peterson• Mature company that markets and manufactures a broad array of
tools for all sectors of the biotechnology industry• Founded in 1987, headquartered in Carlsbad, CA• Went Public in 1999 and acquired 18 different biotech companies
from 1999-2005• Operations in more than 20 countries and distribution in more than
60 countries• Revenue of $1.15 billion in 2006• For further information Invitrogen Web PageAudio and Video Profiles of Invitrogen
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What is Their Core Technology?Tools For Complete Solutions
Products KitsType Title Here
Workflow systemsType Title Here
Technology
75% Drug Discovery Services and 25% Cell CulturesProducts- Pico GreenKits- PathAlertSystems- Qubit or QdotWorkflows- Protein Profiling
PathAlert™ Detection System
Qdot® nanocrystals
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Employment Outlook• Over 4,300 employees
worldwide • 85 job openings in
Carlsbad, CA, for high school graduates to those with graduate degrees
• Some job openings:-scientist -business analyst-patent agent-IT director-accountant-production technician -technical writer-sales and marketing -project manager
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You need to identify your passion and pursue it relentlessly .
“I like to work with my hands”- Al Spada (Conatus)
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• Medical/Pharmaceutical Domain Company– pan caspase inhibitors
• Small molecule drug
• Startup Company– Split from Idun in 2005– Phase 2 clinical studies
(CTS-1027)– Develop a previously
identified potential drug
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Timeline• 2/05: Pfizer acquires Idun• 7/05: Idun management team founded
Conatus• 8/06: New facility & personnel increase• 1-5/07: Secured 55 million in private
funding for phase II clinical studies• 11/07: File IND for phase II clinical
studies• 12/07: Begin clinical studies• 2008: License or collaborate on a
second Liver Disease Drug
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• Pan caspase inhibitor Treatment for liver disease– inflammation – cellular degradation– Viral infection (Hepatitis C)
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LSSI has broken down all the obstacles of bringing biotechnology to the classroom- Victor Rodriguez
(LSSI Teacher)
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www.estudysite.com292 Euclid Avenue, Suite 225
San Diego, CA 92114
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• Inpatient and outpatient medical research site providing phase II through III studies.
• Innovative business model– Utilize emergency department as a gateway for
inpatient and outpatient studies– Vast ethnic and geo-diverse database– Rapid enrollment and retention of a large number of
patients– Partnership with hospitals and physicians– Consistent “on-time” completion
Overview
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Current Studies
• Medical– Infectious Diseases
• Pneumonia • MRSA
– Acute Coronary Syndrome– Hypertension
• Central Nervous System– ADHD– Insomnia– Schizophrenia
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Types of Employment
• Physicians• Registered Nurses• Quality Assurance• Site Manager• Clinical Research
Coordinator• Regulatory Specialist• Pharmacist
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I have had many good changes made by the PCR fairy- Brian Miller(Biogen idec)
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DowpharmaA Business Unit of Dow
44 Biopharma R& D EmployeesComputer ScienceBioinformaticsChemical EngineeringMolecular BiologyMicrobiologyBiochemistry
5501 Oberlin DriveSan Diego California92121 U.S.A.Phone: 800-745-7475 Fax: 858-352-4560
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56
Dow Biopharma Core Technologies
The next generation microbial expression system
Protein expression in P. fluorescens
VLP Vaccine CarrierAdvantaged performance for subunit vaccines
W ild-type VLP Modified VLP displaying a dense array of epitopes
Epitope
Epitope
CP surface loopAntigen W ild-type VLPW ild-type VLP Modified VLP displaying a dense array of epitopes
Epitope
Epitope
CP surface loopAntigen
Post - induction
Pre - induction
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proc
ess a
nalyt
ical
bioinf
orma
tics
syste
ms bi
ology
DNA constructsDNA constructs
strain selectionstrain selection
fermentationfermentation
primary recovery primary recovery
purificationpurification
characterization and analysischaracterization and analysis
UPST
REAM
DOW
NSTR
EAM
MIDS
TREA
M
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It is not what you know, it is how you do your job- Sandi Slivka (Miramar College)
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Sector: Research, Testing and Medical Labs(1000 employees;100,000 worldwide)
Growth Stage: La Jolla – R & D
Including the Pfizer Incubator - a collaboration with Biotechnology startups and ScrippsJuly 18, 2007 Pfizer Announces First Occupant in its Science Incubator
Pfizer - Production and R & D Pfizer is the world's largest privately funded biomedical research organization.
Job Openings: Over 50 jobs ranging from lab technician to associate director positions.
La Jolla
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History: In 1849, cousins Charles Pfizer and Charles Erhardt founded Charles Pfizer & Company in a red brick building in Brooklyn, NY.
Current: Pfizer's roster of drugs, like Lipitor, Celebrex, Viagra, Zoloft, and Zyrtex, grows with the launch of Sutent (developed at La Jolla) , a new, oral, cancer drug treating kidney and
intestinal cancer.
Pfizer Pipeline Portfolio
La Jolla
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Tools & Core Technology
• High throughput chemistry and pharmacology, all types of mass spectrophotometers
• Monoclonal Antibodies• Structural and computational
biology• Cell Cultures • Molecular design• Molecular Biology
• Adaptable• Withstand disappointments
(9/10 drugs will fail)• Multidisciplinary/ Collaborative
Work force• Team Player• Work/Life Balance
Mass Spec and output
What kind of person??
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Be Knowledgeable of future trends for your career path- Jennifer Cayer (Conatus)
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Cells are the Labor
We at “Bio Gen Idec” are the supervisors
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#1 Cancer Therapy World Wide
Non-Hodgkin’s Lymphoma
Rheumatoid Arthritis
Multiple Sclerosis- Relapsing Forms
Crohn’s Disease & Rheumatoid Arthritis
#1 MS Therapeutic Drug
Multiple Sclerosis- Relapsing Forms
Multiple Sclerosis- Monosymptomatic
1st In Class RIA Therapeutic
Non-Hodgkin’s B-Cell lymphoma
Currently Approved Drugs
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Community Outreach• Biogen Idec Foundation &
Corporate Giving- science education and community service programs through grants.
• Externships- high school and college educators gain exposure to the life sciences industry, resources and teaching tools.
• Educational grants to medical schools, hospitals and community based healthcare-related organizations.
• Community Labs- research centers, dedicated to introduce middle and high-school students to hands on biotechnology, and encourage them to seek careers in the biomedical sciences.Since August 2002– 8776 students– 410 Teachers– 308 Employee Volunteers
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Career Opportunities
• Biopharmaceutical Sciences (Manufacturing)
• Finance, Sales & Marketing
• Legal • Medical Affairs
• Medical Research • Research &
Preclinical Development
• Regulatory • Sales & Marketing
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You are the unsung heros, the future scientists are in your hands- Octavio (Dow Pharma)