044 mice mri
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MICE MRIMICE MRI
New Data
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AIMSAIMS
This study is for imaging aortic atherosclerotic plaques in Apo E deficient mice
In addition to study of Apo E atherosclerotic mice this effort is meant to provide necessary technical experiences for rabbit study soon to be approved and transferred to Galveston
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MRI MRI withwith
Contrast (Magnevist)Contrast (Magnevist)In order to be sure that we are looking to the
aorta,we decided to inject contrast media
We had to inject Magnevist to mouse while inside the magnet, so we tried to cannulate femoral vein, jugular vein, intracardiac approach
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In order to decrease the motion effect of respiration,we had to use respiratory gated MRI
To have respiratory gating,we needed to Intubate the mouse and use ventilation
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The MR Image characteristicsThe MR Image characteristics
Coil:5 and 9cmField of view:6 cmMatrix(nv):256Spin Echo:Tr=2500,Te=12, Slice=17-24Movi(while injecting the Gadelenium) :
Tr=200, Te= 5
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3/103/10
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MRI Parameters for MRI Parameters for Movi Images Movi Images
After injection of Magnivest Tr=200, Te=5
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Soft ware analysis to show the Soft ware analysis to show the flow and find the aortaflow and find the aorta
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Spin Echo Images:Spin Echo Images:Tr=2500, Te=12Tr=2500, Te=12
nv=128nv=128
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High resolution spin echo High resolution spin echo imagesimages
Slice thickness :1.6 ,Gap:0.5Slice thickness :1.6 ,Gap:0.5Tr=2500, Te=12, nv=256Tr=2500, Te=12, nv=256
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03/1303/13
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3/14mvcor3/14mvcor
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Sems tranSems tran
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SPIO InjectionSPIO Injection
With injection of Feridex with maximum dose(3mol/kg),we had some problems: because of small blood content of mouse, dilution wasn’t enough
It caused too much decrease in T2 , so although we had approximately good resolution, we think that by decreasing the dose we can find better images
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Future PlansFuture Plans
Injection of 40mol/kg of Feridex
ECG gated MRI (we have the motion effect of heart in chest region)
Starting rabbit study