03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA...

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Kit Components 03/14/2018 Product code Description R2001 ZymoBIOMICS RNA Mini Kit (50 Preps.) Components: R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA Shield (2X Concentrate) - 25 ml & 125 ml; DNA/RNA... R1060-1-50 RNA Lysis Buffer R1060-2-10 RNA Prep Buffer R1003-3-6 RNA Wash Buffer (Concentrate) W1001-1, W1001-4, W1001-6 DNase/RNase Free Water D4300-7-30, D4300-7-3 ZymoBIOMICS HRC Prep Solution E1009-A DNase I E1010-1-4 DNA Digestion Buffer C1058-50, C1058-5 Zymo-Spin III-HRC Filters

Transcript of 03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA...

Page 1: 03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA ... R1060-1-50 RNA Lysis Buffer R1060-2-10

Kit Components03/14/2018

Product code Description

R2001 ZymoBIOMICS RNA Mini Kit (50 Preps.)

Components:

R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNAShield (2X Concentrate) - 25 ml & 125 ml; DNA/RNA...

R1060-1-50 RNA Lysis Buffer

R1060-2-10 RNA Prep Buffer

R1003-3-6 RNA Wash Buffer (Concentrate)

W1001-1, W1001-4, W1001-6 DNase/RNase Free Water

D4300-7-30, D4300-7-3 ZymoBIOMICS HRC Prep Solution

E1009-A DNase I

E1010-1-4 DNA Digestion Buffer

C1058-50, C1058-5 Zymo-Spin III-HRC Filters

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1 Identification

· Product identifier

· Trade name: DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA Shield (2X Concentrate) - 25 ml & 125ml; DNA/RNA Shield Blood Collection Tube, DNA/RNA Shield Fecal Collection Tube, DNA/RNA Shield Collection Tube, DNA/RNA Shield Lysis Tube (Microbe), DNA/RNA Shield LysisTube w/ Swab (Microbe), DNA/RNA Shield Lysis Tube (Tissue); DNA/RNA Shield CollectionTube w/ Swab

· Article number:R1100-50, R1100-250, R1100-8-S, R1200-25, R1200-125, R1150, R1101, R1102, R1103, R1104, R1105, R1106,R1107, R1108, R1109

· Application of the substance / the mixture Laboratory Reagent

· Details of the supplier of the safety data sheet· Manufacturer/Supplier:

Zymo Research Corp.17062 Murphy Ave.Irvine, CA 92614USAPhone: 1-949-679-1190 or [email protected]

· Information department: Product safety department· Emergency telephone number:

During normal business hours (8 am to 5 pm Pacific Standard Time): +1 (949) 679 1190

2 Hazard(s) identification

· Classification of the substance or mixture

GHS07

Acute Tox. 4 H302 Harmful if swallowed.

Skin Irrit. 2 H315 Causes skin irritation.

Eye Irrit. 2B H320 Causes eye irritation.

· Label elements· GHS label elements The product is classified and labeled according to the Globally Harmonized System (GHS).· Hazard pictograms GHS07· Signal word Warning· Hazard statements

Harmful if swallowed.Causes skin and eye irritation.

· Precautionary statementsWash thoroughly after handling.Do not eat, drink or smoke when using this product.Wear protective gloves.If swallowed: Call a poison center/doctor if you feel unwell.

(Contd. on page 2)US

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Trade name: DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA Shield (2X Concentrate) - 25 ml & 125 ml;DNA/RNA Shield Blood Collection Tube, DNA/RNA Shield Fecal Collection Tube, DNA/RNA ShieldCollection Tube, DNA/RNA Shield Lysis Tube (Microbe), DNA/RNA Shield Lysis Tube w/ Swab(Microbe), DNA/RNA Shield Lysis Tube (Tissue); DNA/RNA Shield Collection Tube w/ Swab

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If on skin: Wash with plenty of water.If in eyes: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do.Continue rinsing.Specific treatment (see on this label).Rinse mouth.If skin irritation occurs: Get medical advice/attention.If eye irritation persists: Get medical advice/attention.Take off contaminated clothing and wash it before reuse.Dispose of contents/container in accordance with local/regional/national/international regulations.

· Classification system:· NFPA ratings (scale 0 - 4)

20

0Health = 2Fire = 0Reactivity = 0

· HMIS-ratings (scale 0 - 4)

HEALTH

FIRE

REACTIVITY

2

0

0

Health = 2Fire = 0Reactivity = 0

· Other hazards· Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.

3 Composition/information on ingredients

· Chemical characterization: Mixtures· Description: This product is a proprietary solution.

· Dangerous components: Void

4 First-aid measures

· Description of first aid measures· After inhalation: Supply fresh air; consult doctor in case of complaints.· After skin contact: Immediately wash with water and soap and rinse thoroughly.· After eye contact: Rinse opened eye for several minutes under running water. Then consult a doctor.· After swallowing: Do not induce vomiting; immediately call for medical help.· Information for doctor:· Most important symptoms and effects, both acute and delayed No further relevant information available.

(Contd. on page 3)US

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Trade name: DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA Shield (2X Concentrate) - 25 ml & 125 ml;DNA/RNA Shield Blood Collection Tube, DNA/RNA Shield Fecal Collection Tube, DNA/RNA ShieldCollection Tube, DNA/RNA Shield Lysis Tube (Microbe), DNA/RNA Shield Lysis Tube w/ Swab(Microbe), DNA/RNA Shield Lysis Tube (Tissue); DNA/RNA Shield Collection Tube w/ Swab

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· Indication of any immediate medical attention and special treatment neededNo further relevant information available.

5 Fire-fighting measures

· Extinguishing media· Suitable extinguishing agents:

Not considered a fire hazard. Use extinguishing media appropriate surrounding fire conditions.Use fire fighting measures that suit the environment.

· Advice for firefighters· Protective equipment: Wear protective clothing.

6 Accidental release measures

· Personal precautions, protective equipment and emergency procedures Wear protective clothing.· Environmental precautions: Dilute with plenty of water.· Methods and material for containment and cleaning up:

Ensure adequate ventilation.Dispose contaminated material as waste according to item 13.

· Reference to other sectionsSee Section 7 for information on safe handling.See Section 8 for information on personal protection equipment.See Section 13 for disposal information.

7 Handling and storage

· Handling:· Precautions for safe handling No special precautions are necessary if used correctly.· Information about protection against explosions and fires: No special measures required.

· Conditions for safe storage, including any incompatibilities· Storage:· Requirements to be met by storerooms and receptacles: No special requirements.· Information about storage in one common storage facility: Not required.· Further information about storage conditions: Keep receptacle tightly sealed.· Specific end use(s) Laboratory reagent

8 Exposure controls/personal protection

· Additional information about design of technical systems: No further data; see item 7.(Contd. on page 4)

US

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Trade name: DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA Shield (2X Concentrate) - 25 ml & 125 ml;DNA/RNA Shield Blood Collection Tube, DNA/RNA Shield Fecal Collection Tube, DNA/RNA ShieldCollection Tube, DNA/RNA Shield Lysis Tube (Microbe), DNA/RNA Shield Lysis Tube w/ Swab(Microbe), DNA/RNA Shield Lysis Tube (Tissue); DNA/RNA Shield Collection Tube w/ Swab

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· Control parameters· Components with limit values that require monitoring at the workplace:

The product does not contain any relevant quantities of materials with critical values that have to be monitored atthe workplace.

· Additional information: The lists that were valid during the creation were used as basis.

· Exposure controls· Personal protective equipment:· General protective and hygienic measures: Wash hands before breaks and at the end of work.· Breathing equipment: Not required.· Protection of hands: Use appropiate gloves. Avoid contact with skin.· Penetration time of glove material

The exact break through time has to be found out by the manufacturer of the protective gloves and has to beobserved.

· Eye protection: Avoid contact with eyes.· Body protection:

Avoid contact with eyes, skin, and clothing. Wash throughly after handling. Remove contaminated clothing andwash before use. Avoid ingestion and inhalation.

9 Physical and chemical properties

· Information on basic physical and chemical properties· General Information· Appearance:

Form: LiquidColor: Colorless

· Odor: No information available· Odor threshold: Not determined.

· pH-value: 5.0-7.0

· Change in conditionMelting point/Melting range: Undetermined.Boiling point/Boiling range: Undetermined.

· Flash point: Not applicable.

· Flammability (solid, gaseous): Not applicable.

· Ignition temperature:

Decomposition temperature: Not determined.

· Auto igniting: Product is not selfigniting.

· Danger of explosion: Product does not present an explosion hazard.

(Contd. on page 5)US

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Trade name: DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA Shield (2X Concentrate) - 25 ml & 125 ml;DNA/RNA Shield Blood Collection Tube, DNA/RNA Shield Fecal Collection Tube, DNA/RNA ShieldCollection Tube, DNA/RNA Shield Lysis Tube (Microbe), DNA/RNA Shield Lysis Tube w/ Swab(Microbe), DNA/RNA Shield Lysis Tube (Tissue); DNA/RNA Shield Collection Tube w/ Swab

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· Explosion limits:Lower: Not ApplicableUpper: Not Applicable

· Vapor pressure: Not determined.

· Density: Not determined.· Relative density Not determined.· Vapor density Not determined.· Evaporation rate Not determined.

· Solubility in / Miscibility withWater: Fully miscible.

· Partition coefficient (n-octanol/water): Not determined.

· Viscosity:Dynamic: Not determined.Kinematic: Not determined.

· Solvent content:VOC content: 0,00 %

Solids content: 0,0 %· Other information No further relevant information available.

10 Stability and reactivity

· Reactivity Strong oxidizing agents and acids.· Possibility of hazardous reactions Strong oxidizing agents and acids.· Conditions to avoid Strong oxidizing agents and acids.· Incompatible materials: Strong oxidizing agents and acids.

11 Toxicological information

· Information on toxicological effects· Acute toxicity:· Primary irritant effect:· on the eye: Irritating to eyes.· Additional toxicological information:

· Carcinogenic categories· IARC (International Agency for Research on Cancer)

None of the ingredients is listed.(Contd. on page 6)

US

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Trade name: DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA Shield (2X Concentrate) - 25 ml & 125 ml;DNA/RNA Shield Blood Collection Tube, DNA/RNA Shield Fecal Collection Tube, DNA/RNA ShieldCollection Tube, DNA/RNA Shield Lysis Tube (Microbe), DNA/RNA Shield Lysis Tube w/ Swab(Microbe), DNA/RNA Shield Lysis Tube (Tissue); DNA/RNA Shield Collection Tube w/ Swab

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· NTP (National Toxicology Program)None of the ingredients is listed.

· OSHA-Ca (Occupational Safety & Health Administration)None of the ingredients is listed.

12 Ecological information

· Toxicity· Aquatic toxicity: No further relevant information available.· Persistence and degradability No further relevant information available.· Behavior in environmental systems:· Bioaccumulative potential No further relevant information available.· Mobility in soil No further relevant information available.· Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.· Other adverse effects No further relevant information available.

13 Disposal considerations

· Waste treatment methods· Recommendation:

Dispose of contents in accordance with local/regional/national, and international recommendations.

· Uncleaned packagings:· Recommendation:

Dispose of container in acoordance with local/regional/national and international recommendations.

14 Transport information

· UN-Number Not dangerous goods.· DOT, ADN, IMDG, IATA not regulated

· UN proper shipping name Not dangerous goods.· DOT, ADN, IMDG, IATA not regulated

· Transport hazard class(es) Not dangerous goods.

· DOT, ADN, IMDG, IATA· Class not regulated

· Packing group Not dangerous goods.

(Contd. on page 7)US

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Trade name: DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA Shield (2X Concentrate) - 25 ml & 125 ml;DNA/RNA Shield Blood Collection Tube, DNA/RNA Shield Fecal Collection Tube, DNA/RNA ShieldCollection Tube, DNA/RNA Shield Lysis Tube (Microbe), DNA/RNA Shield Lysis Tube w/ Swab(Microbe), DNA/RNA Shield Lysis Tube (Tissue); DNA/RNA Shield Collection Tube w/ Swab

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· DOT, IMDG, IATA not regulated

· Environmental hazards: Not applicable.

· Special precautions for user Not applicable.

· Transport in bulk according to Annex II ofMARPOL73/78 and the IBC Code Not applicable.

· UN "Model Regulation": not regulated

15 Regulatory information

· Safety, health and environmental regulations/legislation specific for the substance or mixture· Sara· Section 355 (extremely hazardous substances):

None of the ingredients is listed.

· Section 313 (Specific toxic chemical listings):None of the ingredients is listed.

· TSCA (Toxic Substances Control Act):None of the ingredients is listed.

· Proposition 65· Chemicals known to cause cancer:

None of the ingredients is listed.

· Chemicals known to cause reproductive toxicity for females:None of the ingredients is listed.

· Chemicals known to cause reproductive toxicity for males:None of the ingredients is listed.

· Chemicals known to cause developmental toxicity:None of the ingredients is listed.

· Carcinogenic categories· EPA (Environmental Protection Agency)

None of the ingredients is listed.

· TLV (Threshold Limit Value established by ACGIH)None of the ingredients is listed.

· NIOSH-Ca (National Institute for Occupational Safety and Health)None of the ingredients is listed.

· GHS label elements The product is classified and labeled according to the Globally Harmonized System (GHS).· Hazard pictograms GHS07

(Contd. on page 8)US

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Trade name: DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA Shield (2X Concentrate) - 25 ml & 125 ml;DNA/RNA Shield Blood Collection Tube, DNA/RNA Shield Fecal Collection Tube, DNA/RNA ShieldCollection Tube, DNA/RNA Shield Lysis Tube (Microbe), DNA/RNA Shield Lysis Tube w/ Swab(Microbe), DNA/RNA Shield Lysis Tube (Tissue); DNA/RNA Shield Collection Tube w/ Swab

(Contd. of page 7)

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· Signal word Warning· Hazard statements

Harmful if swallowed.Causes skin and eye irritation.

· Precautionary statementsWash thoroughly after handling.Do not eat, drink or smoke when using this product.Wear protective gloves.If swallowed: Call a poison center/doctor if you feel unwell.If on skin: Wash with plenty of water.If in eyes: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do.Continue rinsing.Specific treatment (see on this label).Rinse mouth.If skin irritation occurs: Get medical advice/attention.If eye irritation persists: Get medical advice/attention.Take off contaminated clothing and wash it before reuse.Dispose of contents/container in accordance with local/regional/national/international regulations.

· Chemical safety assessment: A Chemical Safety Assessment has not been carried out.

16 Other informationThis information is based on our present knowledge. However, this shall not constitute a guarantee for any specificproduct features and shall not establish a legally valid contractual relationship.

· Department issuing SDS:Zymo Research Corp.Safety Department17062 Murphy Ave.Irvine, CA 92614USAPhone: 1-949-679-1190 or 1-888-882-9682

· Contact: [email protected]· Date of preparation / last revision 03/14/2018 / -· Abbreviations and acronyms:

ADR: Accord européen sur le transport des marchandises dangereuses par Route (European Agreement concerning the International Carriageof Dangerous Goods by Road)IMDG: International Maritime Code for Dangerous GoodsDOT: US Department of TransportationIATA: International Air Transport AssociationGHS: Globally Harmonised System of Classification and Labelling of ChemicalsEINECS: European Inventory of Existing Commercial Chemical SubstancesELINCS: European List of Notified Chemical SubstancesCAS: Chemical Abstracts Service (division of the American Chemical Society)VOC: Volatile Organic Compounds (USA, EU)PBT: Persistent, Bioaccumulative and ToxicvPvB: very Persistent and very BioaccumulativeTLV: Threshold Limit Value

(Contd. on page 9)US

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Trade name: DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA Shield (2X Concentrate) - 25 ml & 125 ml;DNA/RNA Shield Blood Collection Tube, DNA/RNA Shield Fecal Collection Tube, DNA/RNA ShieldCollection Tube, DNA/RNA Shield Lysis Tube (Microbe), DNA/RNA Shield Lysis Tube w/ Swab(Microbe), DNA/RNA Shield Lysis Tube (Tissue); DNA/RNA Shield Collection Tube w/ Swab

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PEL: Permissible Exposure LimitREL: Recommended Exposure LimitAcute Tox. 4: Acute toxicity – Category 4Skin Irrit. 2: Skin corrosion/irritation – Category 2Eye Irrit. 2B: Serious eye damage/eye irritation – Category 2B

US

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1 Identification

· Product identifier

· Trade name: RNA Lysis Buffer

· Article number: R1060-1-50, R1060-1-100

· Details of the supplier of the safety data sheet· Manufacturer/Supplier:

Zymo Research Corp.17062 Murphy Ave.Irvine, CA 92614USAPhone: 1-949-679-1190 or [email protected]

· Information department: Product safety department· Emergency telephone number:

During normal business hours (8 am to 5 pm Pacific Standard Time): +1 (949) 679 1190

2 Hazard(s) identification

· Classification of the substance or mixture

GHS05 Corrosion

Skin Corr. 1C H314 Causes severe skin burns and eye damage.

Eye Dam. 1 H318 Causes serious eye damage.

GHS07

Acute Tox. 4 H302 Harmful if swallowed.

Acute Tox. 4 H312 Harmful in contact with skin.

Acute Tox. 4 H332 Harmful if inhaled.

Aquatic Chronic 3 H412 Harmful to aquatic life with long lasting effects.

· Label elements· GHS label elements The product is classified and labeled according to the Globally Harmonized System (GHS).· Hazard pictograms GHS05, GHS07· Signal word Danger

· Hazard-determining components of labeling:guanidinium thiocyanate

· Hazard statementsHarmful if swallowed, in contact with skin or if inhaled.Causes severe skin burns and eye damage.Harmful to aquatic life with long lasting effects.

· Precautionary statementsDo not breathe mist/vapours/spray.

(Contd. on page 2)US

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Trade name: RNA Lysis Buffer

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Wash thoroughly after handling.Do not eat, drink or smoke when using this product.Use only outdoors or in a well-ventilated area.Avoid release to the environment.Wear protective gloves/protective clothing/eye protection/face protection.If swallowed: Call a poison center/doctor if you feel unwell.If swallowed: Rinse mouth. Do NOT induce vomiting.If on skin (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower.IF INHALED: Remove person to fresh air and keep comfortable for breathing.If in eyes: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do.Continue rinsing.Immediately call a poison center/doctor.Specific treatment (see on this label).Take off contaminated clothing and wash it before reuse.Wash contaminated clothing before reuse.Store locked up.Dispose of contents/container in accordance with local/regional/national/international regulations.

· Classification system:· NFPA ratings (scale 0 - 4)

30

0Health = 3Fire = 0Reactivity = 0

· HMIS-ratings (scale 0 - 4)

HEALTH

FIRE

REACTIVITY

*3

0

0

Health = *3Fire = 0Reactivity = 0

· Other hazards· Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.

3 Composition/information on ingredients

· Chemical characterization: Mixtures· Description: Mixture of the substances listed below with nonhazardous additions.

· Dangerous components:CAS: 593-84-0 guanidinium thiocyanate ≤70%

CAS: 9002-93-1 Polyethylene glycol octylphenol ether ≤5%

CAS: 137-16-6 N-Lauroylsarcosine, Sodium salt ≤5%US

(Contd. on page 3)

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Trade name: RNA Lysis Buffer

(Contd. of page 2)

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4 First-aid measures

· Description of first aid measures· General information:

Immediately remove any clothing soiled by the product.Symptoms of poisoning may even occur after several hours; therefore medical observation for at least 48 hoursafter the accident.

· After inhalation:Supply fresh air. If required, provide artificial respiration if trained to do so. Keep patient warm. Consult doctor ifsymptoms persist.In case of unconsciousness place patient stably in side position for transportation.

· After skin contact: Immediately wash with water and soap and rinse thoroughly.· After eye contact: Rinse opened eye for several minutes under running water. Then consult a doctor.· After swallowing:

Rinse mouthDo not induce vomiting; immediately call for medical help.

· Information for doctor:· Most important symptoms and effects, both acute and delayed No further relevant information available.· Indication of any immediate medical attention and special treatment needed

No further relevant information available.

5 Fire-fighting measures

· Extinguishing media· Suitable extinguishing agents:

CO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.Use fire fighting measures that suit the environment.

· Advice for firefighters· Protective equipment: Wear self-contained breathing apparatus for fighting fires involving this material

6 Accidental release measures

· Personal precautions, protective equipment and emergency proceduresWear self-contained breathing apparatus for responding to non-incidental release of this material in which there isthe potential for inhalation of vapors, mists or spraysWear protective equipment. Keep unprotected persons away.

· Environmental precautions:Dilute with plenty of water.Do not allow to enter sewers/ surface or ground water.

· Methods and material for containment and cleaning up:Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).Use neutralizing agent.Dispose contaminated material as waste according to item 13.Ensure adequate ventilation.

· Reference to other sectionsSee Section 7 for information on safe handling.

(Contd. on page 4)US

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See Section 8 for information on personal protection equipment.See Section 13 for disposal information.

· Protective Action Criteria for Chemicals· PAC-1:

CAS: 593-84-0 guanidinium thiocyanate 0.98 mg/m³

· PAC-2:CAS: 593-84-0 guanidinium thiocyanate 11 mg/m³

· PAC-3:CAS: 593-84-0 guanidinium thiocyanate 65 mg/m³

7 Handling and storage

· Handling:· Precautions for safe handling

Ensure good ventilation/exhaustion at the workplace.Prevent formation of aerosols.

· Information about protection against explosions and fires: No special measures required.

· Conditions for safe storage, including any incompatibilities· Storage:· Requirements to be met by storerooms and receptacles: No special requirements.· Information about storage in one common storage facility: Do not store together with acids or strong oxidizers· Further information about storage conditions: Keep receptacle tightly sealed.· Specific end use(s) No further relevant information available.

8 Exposure controls/personal protection

· Additional information about design of technical systems: No further data; see item 7.

· Control parametersWork under a chemical fume hood when using this product. Ensure eyewash station and safety showers are readilyaccessible.

· Components with limit values that require monitoring at the workplace:The product does not contain any relevant quantities of materials with critical values that have to be monitored atthe workplace.

· Additional information: The lists that were valid during the creation were used as basis.

· Exposure controlsThe appropriate protective equipment under anticipated circumstances of use include lab-coat, safety glasses withside-shields and gloves.

· Personal protective equipment:· General protective and hygienic measures:

Keep away from foodstuffs, beverages and feed.Immediately remove all soiled and contaminated clothing.Wash hands before breaks and at the end of work.Avoid contact with the eyes and skin.

(Contd. on page 5)US

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· Breathing equipment:In case of brief exposure or low pollution use respiratory filter device. In case of intensive or longer exposure userespiratory protective device that is independent of circulating air.

· Protection of hands:

Protective gloves

The glove material has to be impermeable and resistant to the product/ the substance/ the preparation.Due to missing tests no recommendation to the glove material can be given for the product/ the preparation/ thechemical mixture.Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation

· Material of glovesThe selection of the suitable gloves does not only depend on the material, but also on further marks of quality andvaries from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance ofthe glove material can not be calculated in advance and has therefore to be checked prior to the application.

· Penetration time of glove materialThe exact break through time has to be found out by the manufacturer of the protective gloves and has to beobserved.

· Eye protection:

Tightly sealed goggles

9 Physical and chemical properties

· Information on basic physical and chemical properties· General Information· Appearance:

Form: LiquidColor: Clear

· Odor: Weak, characteristic· Odor threshold: Not determined.

· pH-value at 20 °C (68 °F): 4.8

· Change in conditionMelting point/Melting range: Undetermined.Boiling point/Boiling range: Undetermined.

· Flash point: Not applicable.

· Flammability (solid, gaseous): Not applicable.

· Ignition temperature:

Decomposition temperature: Not determined.

(Contd. on page 6)US

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· Auto igniting: Product is not selfigniting.

· Danger of explosion: Product does not present an explosion hazard.

· Explosion limits:Lower: Not ApplicableUpper: Not Applicable

· Vapor pressure: Not determined.

· Density: Not determined.· Relative density Not determined.· Vapor density Not determined.· Evaporation rate Not determined.

· Solubility in / Miscibility withWater: Fully miscible.

· Partition coefficient (n-octanol/water): Not determined.

· Viscosity:Dynamic: Not determined.Kinematic: Not determined.

· Solvent content:VOC content: 0,00 %

0.0 g/l / 0.00 lb/gl

Solids content: 70,0 %· Other information No further relevant information available.

10 Stability and reactivity

· Reactivity No further relevant information available.· Chemical stability This product is normally stable under anticipated circumstances of use and storage.· Thermal decomposition / conditions to be avoided:

Products of thermal decomposition of this material would include hydrogen cyanide, ammonia, and oxides ofcarbon nitrogen and sulfur.

· Possibility of hazardous reactions No dangerous reactions known.· Conditions to avoid Avoid exposing product to extreme temperatures or incompatible chemicals· Incompatible materials: Acids and strong oxidizers· Hazardous decomposition products:

Product will not undergo self-decomposition, so no such products will be generated.US

(Contd. on page 7)

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(Contd. of page 6)

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11 Toxicological information

· Information on toxicological effects· Acute toxicity:· LD/LC50 values that are relevant for classification:

CAS: 593-84-0 guanidinium thiocyanateOral LD50 593 mg/kg (rat)

· Primary irritant effect:· on the skin: Caustic effect on skin and mucous membranes.· on the eye: Strong caustic effect.· Additional toxicological information:

The product shows the following dangers according to internally approved calculation methods for preparations:HarmfulCorrosiveSwallowing will lead to a strong caustic effect on mouth and throat and to the danger of perforation of esophagusand stomach.

· Carcinogenic categories· IARC (International Agency for Research on Cancer)

None of the ingredients is listed.

· NTP (National Toxicology Program)None of the ingredients is listed.

· OSHA-Ca (Occupational Safety & Health Administration)None of the ingredients is listed.

12 Ecological information

· Toxicity· Aquatic toxicity:

CAS: 593-84-0 guanidinium thiocyanateEC50 42.4 mg/kg (daphnia)

· Persistence and degradability No further relevant information available.· Behavior in environmental systems:· Bioaccumulative potential No further relevant information available.· Mobility in soil No further relevant information available.· Additional ecological information:· General notes:

Water hazard class 2 (Self-assessment): hazardous for waterDo not allow product to reach ground water, water course or sewage system.Must not reach bodies of water or drainage ditch undiluted or unneutralized.Danger to drinking water if even small quantities leak into the ground.

· Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.

(Contd. on page 8)US

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· Other adverse effects No further relevant information available.

13 Disposal considerations

· Waste treatment methods· Recommendation:

Dispose of contents in accordance with local/regional/national, and international recommendations.

· Uncleaned packagings:· Recommendation:

Dispose of container in acoordance with local/regional/national and international recommendations.· Recommended cleansing agent: Water, if necessary with cleansing agents.

14 Transport information

· UN-Number· DOT, IMDG, IATA UN3265

· UN proper shipping name· DOT Corrosive liquid, acidic, organic, n.o.s. (guanidinium thiocyanate)· IMDG, IATA CORROSIVE LIQUID, ACIDIC, ORGANIC, N.O.S. (guanidinium

thiocyanate)

· Transport hazard class(es)

· DOT

· Class 8 Corrosive substances· Label 8

· IMDG, IATA

· Class 8 Corrosive substances· Label 8

· Packing group· DOT, IMDG, IATA III

· Environmental hazards: Not applicable.

· Special precautions for user Warning: Corrosive substances· Danger code (Kemler): 80

(Contd. on page 9)US

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· EMS Number: F-A,S-B· Segregation groups Acids· Stowage Category A· Stowage Code SW2 Clear of living quarters.

· Transport in bulk according to Annex II ofMARPOL73/78 and the IBC Code Not applicable.

· Transport/Additional information:

· DOT· Quantity limitations On passenger aircraft/rail: 5 L

On cargo aircraft only: 60 L

· IMDG· Limited quantities (LQ) 5L· Excepted quantities (EQ) Code: E1

Maximum net quantity per inner packaging: 30 mlMaximum net quantity per outer packaging: 1000 ml

· UN "Model Regulation": UN 3265 CORROSIVE LIQUID, ACIDIC, ORGANIC, N.O.S.(GUANIDINIUM THIOCYANATE), 8, III

15 Regulatory information

· Safety, health and environmental regulations/legislation specific for the substance or mixture· Sara· Section 355 (extremely hazardous substances):

None of the ingredients is listed.

· Section 313 (Specific toxic chemical listings):None of the ingredients is listed.

· TSCA (Toxic Substances Control Act):All ingredients are listed.

· Proposition 65· Chemicals known to cause cancer:

None of the ingredients is listed.

· Chemicals known to cause reproductive toxicity for females:None of the ingredients is listed.

· Chemicals known to cause reproductive toxicity for males:None of the ingredients is listed.

· Chemicals known to cause developmental toxicity:None of the ingredients is listed.

(Contd. on page 10)US

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(Contd. of page 9)

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· Carcinogenic categories· EPA (Environmental Protection Agency)

None of the ingredients is listed.

· TLV (Threshold Limit Value established by ACGIH)None of the ingredients is listed.

· NIOSH-Ca (National Institute for Occupational Safety and Health)None of the ingredients is listed.

· GHS label elements The product is classified and labeled according to the Globally Harmonized System (GHS).· Hazard pictograms GHS05, GHS07· Signal word Danger

· Hazard-determining components of labeling:guanidinium thiocyanate

· Hazard statementsHarmful if swallowed, in contact with skin or if inhaled.Causes severe skin burns and eye damage.Harmful to aquatic life with long lasting effects.

· Precautionary statementsDo not breathe mist/vapours/spray.Wash thoroughly after handling.Do not eat, drink or smoke when using this product.Use only outdoors or in a well-ventilated area.Avoid release to the environment.Wear protective gloves/protective clothing/eye protection/face protection.If swallowed: Call a poison center/doctor if you feel unwell.If swallowed: Rinse mouth. Do NOT induce vomiting.If on skin (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower.IF INHALED: Remove person to fresh air and keep comfortable for breathing.If in eyes: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do.Continue rinsing.Immediately call a poison center/doctor.Specific treatment (see on this label).Take off contaminated clothing and wash it before reuse.Wash contaminated clothing before reuse.Store locked up.Dispose of contents/container in accordance with local/regional/national/international regulations.

· Chemical safety assessment: A Chemical Safety Assessment has not been carried out.

16 Other informationThis information is based on our present knowledge. However, this shall not constitute a guarantee for any specificproduct features and shall not establish a legally valid contractual relationship.

· Department issuing SDS:Zymo Research Corp.Safety Department17062 Murphy Ave.Irvine, CA 92614

(Contd. on page 11)US

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USAPhone: 1-949-679-1190 or 1-888-882-9682

· Contact: [email protected]· Date of preparation / last revision 03/14/2018 / -· Abbreviations and acronyms:

ADR: Accord européen sur le transport des marchandises dangereuses par Route (European Agreement concerning the International Carriageof Dangerous Goods by Road)IMDG: International Maritime Code for Dangerous GoodsDOT: US Department of TransportationIATA: International Air Transport AssociationGHS: Globally Harmonised System of Classification and Labelling of ChemicalsEINECS: European Inventory of Existing Commercial Chemical SubstancesELINCS: European List of Notified Chemical SubstancesCAS: Chemical Abstracts Service (division of the American Chemical Society)VOC: Volatile Organic Compounds (USA, EU)LC50: Lethal concentration, 50 percentLD50: Lethal dose, 50 percentPBT: Persistent, Bioaccumulative and ToxicSVHC: Substances of Very High ConcernvPvB: very Persistent and very BioaccumulativeTLV: Threshold Limit ValuePEL: Permissible Exposure LimitREL: Recommended Exposure LimitAcute Tox. 4: Acute toxicity – Category 4Skin Corr. 1C: Skin corrosion/irritation – Category 1CEye Dam. 1: Serious eye damage/eye irritation – Category 1Aquatic Chronic 3: Hazardous to the aquatic environment - long-term aquatic hazard – Category 3

US

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1 Identification

· Product identifier

· Trade name: RNA Prep Buffer

· Article number: R1060-2-10, R1060-2-25, R1060-2-100· Application of the substance / the mixture Laboratory Reagent

· Details of the supplier of the safety data sheet· Manufacturer/Supplier:

Zymo Research Corp.17062 Murphy Ave.Irvine, CA 92614USAPhone: 1-949-679-1190 or [email protected]

· Information department: Product safety department· Emergency telephone number:

During normal business hours (8 am to 5 pm Pacific Standard Time): +1 (949) 679 1190

2 Hazard(s) identification

· Classification of the substance or mixture

GHS02 Flame

Flam. Liq. 2 H225 Highly flammable liquid and vapor.

GHS07

Acute Tox. 4 H302 Harmful if swallowed.

Skin Irrit. 2 H315 Causes skin irritation.

Eye Irrit. 2A H319 Causes serious eye irritation.

· Label elements· GHS label elements The product is classified and labeled according to the Globally Harmonized System (GHS).· Hazard pictograms GHS02, GHS07· Signal word Danger

· Hazard-determining components of labeling:guanidinium chlorideethanol

· Hazard statementsHighly flammable liquid and vapor.Harmful if swallowed.Causes skin irritation.Causes serious eye irritation.

· Precautionary statementsKeep away from heat/sparks/open flames/hot surfaces. - No smoking.

(Contd. on page 2)US

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Keep container tightly closed.Ground/bond container and receiving equipment.Use explosion-proof electrical/ventilating/lighting/equipment.Use only non-sparking tools.Take precautionary measures against static discharge.Wash thoroughly after handling.Do not eat, drink or smoke when using this product.Wear protective gloves / eye protection / face protection.If swallowed: Call a poison center/doctor if you feel unwell.If on skin (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower.If in eyes: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do.Continue rinsing.Specific treatment (see on this label).Rinse mouth.If skin irritation occurs: Get medical advice/attention.If eye irritation persists: Get medical advice/attention.Take off contaminated clothing and wash it before reuse.In case of fire: Use for extinction: CO2, powder or water spray.Store in a well-ventilated place. Keep cool.Dispose of contents/container in accordance with local/regional/national/international regulations.

· Classification system:· NFPA ratings (scale 0 - 4)

23

0Health = 2Fire = 3Reactivity = 0

· HMIS-ratings (scale 0 - 4)

HEALTH

FIRE

REACTIVITY

2

3

0

Health = 2Fire = 3Reactivity = 0

· Other hazards· Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.

3 Composition/information on ingredients

· Chemical characterization: Mixtures· Description: Mixture of the substances listed below with nonhazardous additions.

· Dangerous components:CAS: 64-17-5 ethanol 100%

CAS: 50-01-1 guanidinium chloride 40%US

(Contd. on page 3)

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4 First-aid measures

· Description of first aid measures· General information:

Symptoms of poisoning may even occur after several hours; therefore medical observation for at least 48 hoursafter the accident.

· After inhalation: In case of unconsciousness place patient stably in side position for transportation.· After skin contact: Immediately wash with water and soap and rinse thoroughly.· After eye contact:

Rinse opened eye for several minutes under running water. If symptoms persist, consult a doctor.· After swallowing: Do not induce vomiting; immediately call for medical help.· Information for doctor:· Most important symptoms and effects, both acute and delayed No further relevant information available.· Indication of any immediate medical attention and special treatment needed

No further relevant information available.

5 Fire-fighting measures

· Extinguishing media· Suitable extinguishing agents:

CO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.· Advice for firefighters· Protective equipment: Wear protective clothing.

6 Accidental release measures

· Personal precautions, protective equipment and emergency proceduresWear protective equipment. Keep unprotected persons away.

· Environmental precautions:Prevent seepage into sewage system, workpits and cellars.Dilute with plenty of water.

· Methods and material for containment and cleaning up:Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).Dispose contaminated material as waste according to item 13.Ensure adequate ventilation.

· Reference to other sectionsSee Section 7 for information on safe handling.See Section 8 for information on personal protection equipment.See Section 13 for disposal information.

· Protective Action Criteria for Chemicals· PAC-1:

CAS: 64-17-5 ethanol 1,800 ppm

CAS: 50-01-1 guanidinium chloride 1.4 mg/m3(Contd. on page 4)

US

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· PAC-2:CAS: 64-17-5 ethanol 3300* ppm

CAS: 50-01-1 guanidinium chloride 16 mg/m3

· PAC-3:CAS: 64-17-5 ethanol 15000* ppm

CAS: 50-01-1 guanidinium chloride 94 mg/m3

7 Handling and storage

· Handling:· Precautions for safe handling

Ensure good ventilation/exhaustion at the workplace.Prevent formation of aerosols.

· Information about protection against explosions and fires:Keep ignition sources away - Do not smoke.Protect against electrostatic charges.

· Conditions for safe storage, including any incompatibilities· Storage:· Requirements to be met by storerooms and receptacles: Store in a cool location.· Information about storage in one common storage facility: Not required.· Further information about storage conditions:

Keep receptacle tightly sealed.Store in cool, dry conditions in well sealed receptacles.

· Specific end use(s) Laboratory reagent

8 Exposure controls/personal protection

· Additional information about design of technical systems: No further data; see item 7.

· Control parameters· Components with limit values that require monitoring at the workplace:

The following constituent is the only constituent of the product which has a PEL, TLV or other recommendedexposure limit.At this time, the remaining constituent has no known exposure limits.

CAS: 64-17-5 ethanolPEL Long-term value: 1900 mg/m³, 1000 ppm

REL Long-term value: 1900 mg/m³, 1000 ppm

TLV Short-term value: 1880 mg/m³, 1000 ppm

· Additional information: The lists that were valid during the creation were used as basis.

· Exposure controls· Personal protective equipment:· General protective and hygienic measures:

Keep away from foodstuffs, beverages and feed.(Contd. on page 5)

US

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Immediately remove all soiled and contaminated clothing.Wash hands before breaks and at the end of work.Avoid contact with the eyes and skin.

· Breathing equipment:In case of brief exposure or low pollution use respiratory filter device. In case of intensive or longer exposure userespiratory protective device that is independent of circulating air.

· Protection of hands:

Protective gloves

The glove material has to be impermeable and resistant to the product/ the substance/ the preparation.Due to missing tests no recommendation to the glove material can be given for the product/ the preparation/ thechemical mixture.Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation

· Material of glovesThe selection of the suitable gloves does not only depend on the material, but also on further marks of quality andvaries from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance ofthe glove material can not be calculated in advance and has therefore to be checked prior to the application.

· Penetration time of glove materialThe exact break through time has to be found out by the manufacturer of the protective gloves and has to beobserved.

· Eye protection:

Tightly sealed goggles

9 Physical and chemical properties

· Information on basic physical and chemical properties· General Information· Appearance:

Form: LiquidColor: Yellow tint

· Odor: Odorless· Odor threshold: Not determined.

· pH-value: Not determined.

· Change in conditionMelting point/Melting range: Undetermined.Boiling point/Boiling range: Undetermined.

· Flash point: 13 °C (55.4 °F)

· Flammability (solid, gaseous): Not applicable.

(Contd. on page 6)US

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· Ignition temperature: 425 °C (797 °F)

· Decomposition temperature: Not determined.

· Auto igniting: Product is not selfigniting.

· Danger of explosion: Product is not explosive. However, formation of explosive air/vapormixtures are possible.

· Explosion limits:Lower: 3,5 Vol %Upper: 15,0 Vol %

· Vapor pressure at 20 °C (68 °F): 59 hPa (44.3 mm Hg)

· Density: Not determined.· Relative density Not determined.· Vapor density Not determined.· Evaporation rate Not determined.

· Solubility in / Miscibility withWater: Fully miscible.

· Partition coefficient (n-octanol/water): Not determined.

· Viscosity:Dynamic: Not determined.Kinematic: Not determined.

· Solvent content:Organic solvents: 80,0 %VOC content: 80,0 %

800.0 g/l / 6.68 lb/gl· Other information No further relevant information available.

10 Stability and reactivity

· Reactivity No further relevant information available.· Chemical stability· Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.· Possibility of hazardous reactions No dangerous reactions known.· Conditions to avoid No further relevant information available.· Incompatible materials: No further relevant information available.· Hazardous decomposition products: No dangerous decomposition products known.

US

(Contd. on page 7)

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11 Toxicological information

· Information on toxicological effects· Acute toxicity:· LD/LC50 values that are relevant for classification:

CAS: 50-01-1 guanidinium chlorideOral LD50 475 mg/kg (rat)

· Primary irritant effect:· on the skin: Irritant to skin and mucous membranes.· on the eye: Irritating effect.· Additional toxicological information:

The product shows the following dangers according to internally approved calculation methods for preparations:HarmfulIrritant

· Carcinogenic categories· IARC (International Agency for Research on Cancer)

None of the ingredients is listed.

· NTP (National Toxicology Program)None of the ingredients is listed.

· OSHA-Ca (Occupational Safety & Health Administration)None of the ingredients is listed.

12 Ecological information

· Toxicity· Aquatic toxicity: No further relevant information available.· Persistence and degradability No further relevant information available.· Behavior in environmental systems:· Bioaccumulative potential No further relevant information available.· Mobility in soil No further relevant information available.· Additional ecological information:· General notes: Not known to be hazardous to water.· Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.· Other adverse effects No further relevant information available.

13 Disposal considerations

· Waste treatment methods· Recommendation:

Dispose of contents in accordance with local/regional/national, and international recommendations.(Contd. on page 8)

US

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· Uncleaned packagings:· Recommendation:

Dispose of container in acoordance with local/regional/national and international recommendations.· Recommended cleansing agent: Water, if necessary with cleansing agents.

14 Transport information

· UN-Number· DOT, IMDG, IATA UN1170

· UN proper shipping name· DOT Ethanol mixture· IMDG ETHANOL (ETHYL ALCOHOL) mixture· IATA ETHANOL mixture

· Transport hazard class(es)

· DOT

· Class 3 Flammable liquids· Label 3

· IMDG, IATA

· Class 3 Flammable liquids· Label 3

· Packing group· DOT, IMDG, IATA II

· Environmental hazards: Not applicable.

· Special precautions for user Warning: Flammable liquids· Danger code (Kemler): 33· EMS Number: F-E,S-D· Stowage Category A

· Transport in bulk according to Annex II ofMARPOL73/78 and the IBC Code Not applicable.

(Contd. on page 9)US

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· Transport/Additional information:

· DOT· Quantity limitations On passenger aircraft/rail: 5 L

On cargo aircraft only: 60 L

· IMDG· Limited quantities (LQ) 1L· Excepted quantities (EQ) Code: E2

Maximum net quantity per inner packaging: 30 mlMaximum net quantity per outer packaging: 500 ml

· UN "Model Regulation": UN 1170 ETHANOL MIXTURE, 3, II

15 Regulatory information

· Safety, health and environmental regulations/legislation specific for the substance or mixture· Sara· Section 355 (extremely hazardous substances):

None of the ingredients is listed.

· Section 313 (Specific toxic chemical listings):None of the ingredients is listed.

· TSCA (Toxic Substances Control Act):All ingredients are listed.

· Proposition 65· Chemicals known to cause cancer:

None of the ingredients is listed.

· Chemicals known to cause reproductive toxicity for females:None of the ingredients is listed.

· Chemicals known to cause reproductive toxicity for males:None of the ingredients is listed.

· Chemicals known to cause developmental toxicity:None of the ingredients is listed.

· Carcinogenic categories· EPA (Environmental Protection Agency)

None of the ingredients is listed.

· TLV (Threshold Limit Value established by ACGIH)CAS: 64-17-5 ethanol A3

· NIOSH-Ca (National Institute for Occupational Safety and Health)None of the ingredients is listed.

· GHS label elements The product is classified and labeled according to the Globally Harmonized System (GHS).(Contd. on page 10)

US

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· Hazard pictograms GHS02, GHS07· Signal word Danger

· Hazard-determining components of labeling:guanidinium chlorideethanol

· Hazard statementsHighly flammable liquid and vapor.Harmful if swallowed.Causes skin irritation.Causes serious eye irritation.

· Precautionary statementsKeep away from heat/sparks/open flames/hot surfaces. - No smoking.Keep container tightly closed.Ground/bond container and receiving equipment.Use explosion-proof electrical/ventilating/lighting/equipment.Use only non-sparking tools.Take precautionary measures against static discharge.Wash thoroughly after handling.Do not eat, drink or smoke when using this product.Wear protective gloves / eye protection / face protection.If swallowed: Call a poison center/doctor if you feel unwell.If on skin (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower.If in eyes: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do.Continue rinsing.Specific treatment (see on this label).Rinse mouth.If skin irritation occurs: Get medical advice/attention.If eye irritation persists: Get medical advice/attention.Take off contaminated clothing and wash it before reuse.In case of fire: Use for extinction: CO2, powder or water spray.Store in a well-ventilated place. Keep cool.Dispose of contents/container in accordance with local/regional/national/international regulations.

· Chemical safety assessment: A Chemical Safety Assessment has not been carried out.

16 Other informationThis information is based on our present knowledge. However, this shall not constitute a guarantee for any specificproduct features and shall not establish a legally valid contractual relationship.

· Department issuing SDS:Zymo Research Corp.Safety Department17062 Murphy Ave.Irvine, CA 92614USAPhone: 1-949-679-1190 or 1-888-882-9682

· Contact: [email protected]· Date of preparation / last revision 03/14/2018 / -

(Contd. on page 11)US

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Page 11/11

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Printing date 03/14/2018 Reviewed on 12/16/2016

Trade name: RNA Prep Buffer

(Contd. of page 10)

46.0

· Abbreviations and acronyms:ADR: Accord européen sur le transport des marchandises dangereuses par Route (European Agreement concerning the International Carriageof Dangerous Goods by Road)IMDG: International Maritime Code for Dangerous GoodsIATA: International Air Transport AssociationGHS: Globally Harmonised System of Classification and Labelling of ChemicalsEINECS: European Inventory of Existing Commercial Chemical SubstancesELINCS: European List of Notified Chemical SubstancesCAS: Chemical Abstracts Service (division of the American Chemical Society)VOC: Volatile Organic Compounds (USA, EU)LC50: Lethal concentration, 50 percentLD50: Lethal dose, 50 percentPBT: Persistent, Bioaccumulative and ToxicvPvB: very Persistent and very BioaccumulativeTLV: Threshold Limit ValuePEL: Permissible Exposure LimitREL: Recommended Exposure LimitFlam. Liq. 2: Flammable liquids – Category 2Acute Tox. 4: Acute toxicity – Category 4Skin Irrit. 2: Skin corrosion/irritation – Category 2Eye Irrit. 2A: Serious eye damage/eye irritation – Category 2A

US

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1 Identification

· Product identifier

· Trade name: RNA Wash Buffer (Concentrate)

· Article number: R1003-3-6, R1003-3-12, R1003-3-24, R1003-3-48· Application of the substance / the mixture Laboratory Reagent

· Details of the supplier of the safety data sheet· Manufacturer/Supplier:

Zymo Research Corp.17062 Murphy Ave.Irvine, CA 92614USAPhone: 1-949-679-1190 or [email protected]

· Information department: Product safety department· Emergency telephone number:

During normal business hours (8 am to 5 pm Pacific Standard Time): +1 (949) 679 1190

2 Hazard(s) identification

· Classification of the substance or mixtureThe product is not classified according to the Globally Harmonized System (GHS).

· Label elements· GHS label elements Void· Hazard pictograms Void· Signal word Void· Hazard statements Void· Classification system:· NFPA ratings (scale 0 - 4)

00

0Health = 0Fire = 0Reactivity = 0

· HMIS-ratings (scale 0 - 4)

HEALTH

FIRE

REACTIVITY

0

0

0

Health = 0Fire = 0Reactivity = 0

· Other hazards· Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.

US

(Contd. on page 2)

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Trade name: RNA Wash Buffer (Concentrate)

(Contd. of page 1)

46.0

3 Composition/information on ingredients

· Chemical characterization: Mixtures· Description: Mixture of the substances listed below with nonhazardous additions.

· Dangerous components: Void

4 First-aid measures

· Description of first aid measures· General information: No special measures required.· After inhalation: Supply fresh air; consult doctor in case of complaints.· After skin contact: Generally the product does not irritate the skin.· After eye contact: Rinse opened eye for several minutes under running water. Then consult a doctor.· After swallowing: Do not induce vomiting; immediately call for medical help.· Information for doctor:· Most important symptoms and effects, both acute and delayed No further relevant information available.· Indication of any immediate medical attention and special treatment needed

No further relevant information available.

5 Fire-fighting measures

· Extinguishing media· Suitable extinguishing agents:

CO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.Use fire fighting measures that suit the environment.

· Advice for firefighters· Protective equipment: Wear protective clothing.

6 Accidental release measures

· Personal precautions, protective equipment and emergency procedures Wear protective clothing.· Environmental precautions:

Dilute with plenty of water.Do not allow to enter sewers/ surface or ground water.

· Methods and material for containment and cleaning up:Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).

· Reference to other sectionsSee Section 7 for information on safe handling.See Section 8 for information on personal protection equipment.See Section 13 for disposal information.

· Protective Action Criteria for Chemicals· PAC-1:

CAS: 1185-53-1 2-amino-2-(hydroxymethyl)propane-1,3-diolhydrochloride 12 mg/m³(Contd. on page 3)

US

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Trade name: RNA Wash Buffer (Concentrate)

(Contd. of page 2)

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CAS: 6381-92-6 Edetate Disodium, Dihydrate 30 mg/m³

· PAC-2:CAS: 1185-53-1 2-amino-2-(hydroxymethyl)propane-1,3-diolhydrochloride 130 mg/m³

CAS: 6381-92-6 Edetate Disodium, Dihydrate 330 mg/m³

· PAC-3:CAS: 1185-53-1 2-amino-2-(hydroxymethyl)propane-1,3-diolhydrochloride 790 mg/m³

CAS: 6381-92-6 Edetate Disodium, Dihydrate 2,000 mg/m³

7 Handling and storage

· Handling:· Precautions for safe handling No special measures required.· Information about protection against explosions and fires: No special measures required.

· Conditions for safe storage, including any incompatibilities· Storage:· Requirements to be met by storerooms and receptacles: No special requirements.· Information about storage in one common storage facility: Not required.· Further information about storage conditions: None.· Specific end use(s) Laboratory reagent

8 Exposure controls/personal protection

· Additional information about design of technical systems: No further data; see item 7.

· Control parameters· Components with limit values that require monitoring at the workplace:

The product does not contain any relevant quantities of materials with critical values that have to be monitored atthe workplace.

· Additional information: The lists that were valid during the creation were used as basis.

· Exposure controls· Personal protective equipment:· General protective and hygienic measures:

The usual precautionary measures for handling chemicals should be followed.· Breathing equipment: Not required.· Protection of hands:

The glove material has to be impermeable and resistant to the product/ the substance/ the preparation.Due to missing tests no recommendation to the glove material can be given for the product/ the preparation/ thechemical mixture.Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation

· Material of glovesThe selection of the suitable gloves does not only depend on the material, but also on further marks of quality andvaries from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance ofthe glove material can not be calculated in advance and has therefore to be checked prior to the application.

(Contd. on page 4)US

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Trade name: RNA Wash Buffer (Concentrate)

(Contd. of page 3)

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· Penetration time of glove materialThe exact break through time has to be found out by the manufacturer of the protective gloves and has to beobserved.

· Eye protection: Goggles recommended during refilling.

9 Physical and chemical properties

· Information on basic physical and chemical properties· General Information· Appearance:

Form: LiquidColor: Clear

· Odor: Odorless· Odor threshold: Not determined.

· pH-value: Not determined.

· Change in conditionMelting point/Melting range: Undetermined.Boiling point/Boiling range: Undetermined.

· Flash point: Not applicable.

· Flammability (solid, gaseous): Not applicable.

· Ignition temperature:

Decomposition temperature: Not determined.

· Auto igniting: Product is not selfigniting.

· Danger of explosion: Product does not present an explosion hazard.

· Explosion limits:Lower: Not ApplicableUpper: Not Applicable

· Vapor pressure: Not determined.

· Density: Not determined.· Relative density Not determined.· Vapor density Not determined.· Evaporation rate Not determined.

· Solubility in / Miscibility withWater: Fully miscible.

· Partition coefficient (n-octanol/water): Not determined.

· Viscosity:Dynamic: Not determined.Kinematic: Not determined.

(Contd. on page 5)US

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Trade name: RNA Wash Buffer (Concentrate)

(Contd. of page 4)

46.0

· Solvent content:VOC content: 0.00 %

0.0 g/l / 0.00 lb/gl

Solids content: 2.0 %· Other information No further relevant information available.

10 Stability and reactivity

· Reactivity No further relevant information available.· Chemical stability· Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.· Possibility of hazardous reactions No dangerous reactions known.· Conditions to avoid No further relevant information available.· Incompatible materials: No further relevant information available.· Hazardous decomposition products: No dangerous decomposition products known.

11 Toxicological information

· Information on toxicological effects· Acute toxicity:· Primary irritant effect:· on the skin: No irritant effect.· on the eye: No irritating effect.· Sensitization: No sensitizing effects known.· Additional toxicological information:

The product is not subject to classification according to internally approved calculation methods for preparations:When used and handled according to specifications, the product does not have any harmful effects according toour experience and the information provided to us.

· Carcinogenic categories· IARC (International Agency for Research on Cancer)

None of the ingredients is listed.

· NTP (National Toxicology Program)None of the ingredients is listed.

· OSHA-Ca (Occupational Safety & Health Administration)None of the ingredients is listed.

12 Ecological information

· Toxicity· Aquatic toxicity: No further relevant information available.· Persistence and degradability No further relevant information available.

(Contd. on page 6)US

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Trade name: RNA Wash Buffer (Concentrate)

(Contd. of page 5)

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· Behavior in environmental systems:· Bioaccumulative potential No further relevant information available.· Mobility in soil No further relevant information available.· Additional ecological information:· General notes:

Water hazard class 1 (Self-assessment): slightly hazardous for waterDo not allow undiluted product or large quantities of it to reach ground water, water course or sewage system.

· Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.· Other adverse effects No further relevant information available.

13 Disposal considerations

· Waste treatment methods· Recommendation: Smaller quantities can be disposed of with household waste.

· Uncleaned packagings:· Recommendation:

Dispose of container in acoordance with local/regional/national and international recommendations.· Recommended cleansing agent: Water, if necessary with cleansing agents.

14 Transport information

· UN-Number· DOT, ADN, IMDG, IATA not regulated

· UN proper shipping name· DOT, ADN, IMDG, IATA not regulated

· Transport hazard class(es)

· DOT, ADN, IMDG, IATA· Class not regulated

· Packing group· DOT, IMDG, IATA not regulated

· Environmental hazards: Not applicable.

· Special precautions for user Not applicable.

· Transport in bulk according to Annex II ofMARPOL73/78 and the IBC Code Not applicable.

· UN "Model Regulation": not regulated

US

(Contd. on page 7)

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Trade name: RNA Wash Buffer (Concentrate)

(Contd. of page 6)

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15 Regulatory information

· Safety, health and environmental regulations/legislation specific for the substance or mixture· Sara· Section 355 (extremely hazardous substances):

None of the ingredients is listed.

· Section 313 (Specific toxic chemical listings):None of the ingredients is listed.

· TSCA (Toxic Substances Control Act):CAS: 1185-53-1 2-amino-2-(hydroxymethyl)propane-1,3-diolhydrochloride

· TSCA new (21st Century Act) (Substances not listed)· Proposition 65· Chemicals known to cause cancer:

None of the ingredients is listed.

· Chemicals known to cause reproductive toxicity for females:None of the ingredients is listed.

· Chemicals known to cause reproductive toxicity for males:None of the ingredients is listed.

· Chemicals known to cause developmental toxicity:None of the ingredients is listed.

· Carcinogenic categories· EPA (Environmental Protection Agency)

None of the ingredients is listed.

· TLV (Threshold Limit Value established by ACGIH)None of the ingredients is listed.

· NIOSH-Ca (National Institute for Occupational Safety and Health)None of the ingredients is listed.

· GHS label elements Void· Hazard pictograms Void· Signal word Void· Hazard statements Void· Chemical safety assessment: A Chemical Safety Assessment has not been carried out.

16 Other informationThis information is based on our present knowledge. However, this shall not constitute a guarantee for any specificproduct features and shall not establish a legally valid contractual relationship.

· Department issuing SDS:Zymo Research Corp.Safety Department17062 Murphy Ave.Irvine, CA 92614

(Contd. on page 8)US

Page 40: 03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA ... R1060-1-50 RNA Lysis Buffer R1060-2-10

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Trade name: RNA Wash Buffer (Concentrate)

(Contd. of page 7)

46.0

USAPhone: 1-949-679-1190 or 1-888-882-9682

· Contact: [email protected]· Date of preparation / last revision 03/14/2018 / -· Abbreviations and acronyms:

ADR: Accord européen sur le transport des marchandises dangereuses par Route (European Agreement concerning the International Carriageof Dangerous Goods by Road)IMDG: International Maritime Code for Dangerous GoodsDOT: US Department of TransportationIATA: International Air Transport AssociationACGIH: American Conference of Governmental Industrial HygienistsEINECS: European Inventory of Existing Commercial Chemical SubstancesELINCS: European List of Notified Chemical SubstancesCAS: Chemical Abstracts Service (division of the American Chemical Society)NFPA: National Fire Protection Association (USA)HMIS: Hazardous Materials Identification System (USA)VOC: Volatile Organic Compounds (USA, EU)PBT: Persistent, Bioaccumulative and ToxicvPvB: very Persistent and very BioaccumulativeNIOSH: National Institute for Occupational SafetyOSHA: Occupational Safety & HealthTLV: Threshold Limit ValuePEL: Permissible Exposure LimitREL: Recommended Exposure Limit

US

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46.0

1 Identification

· Product identifier

· Trade name: DNase/RNase Free Water

· Article number: W1001-1, W1001-4, W1001-6, W1001-10, W1001-30, W1001-100, W1001-200· CAS Number:

7732-18-5· EC number:

231-791-2· Application of the substance / the mixture Laboratory Reagent

· Details of the supplier of the safety data sheet· Manufacturer/Supplier:

Zymo Research Corp.17062 Murphy Ave.Irvine, CA 92614USAPhone: 1-949-679-1190 or [email protected]

· Information department: Product safety department· Emergency telephone number:

During normal business hours (8 am to 5 pm Pacific Standard Time): +1 (949) 679 1190

2 Hazard(s) identification

· Classification of the substance or mixtureThe substance is not classified according to the Globally Harmonized System (GHS).

· Label elements· GHS label elements Void· Hazard pictograms Void· Signal word Void· Hazard statements Void· Classification system:· NFPA ratings (scale 0 - 4)

00

0Health = 0Fire = 0Reactivity = 0

· HMIS-ratings (scale 0 - 4)

HEALTH

FIRE

REACTIVITY

0

0

0

Health = 0Fire = 0Reactivity = 0

· Other hazards· Results of PBT and vPvB assessment· PBT: Not applicable.

(Contd. on page 2)US

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Trade name: DNase/RNase Free Water

(Contd. of page 1)

46.0

· vPvB: Not applicable.

3 Composition/information on ingredients

· Chemical characterization: Substances· CAS No. Description

7732-18-5 water, distilled, conductivity or of similar purity· Identification number(s)· EC number: 231-791-2

4 First-aid measures

· Description of first aid measures· General information: No special measures required.· After inhalation: Supply fresh air; consult doctor in case of complaints.· After skin contact: Generally the product does not irritate the skin.· After eye contact: Rinse opened eye for several minutes under running water. Then consult a doctor.· After swallowing: Do not induce vomiting; immediately call for medical help.· Information for doctor:· Most important symptoms and effects, both acute and delayed No further relevant information available.· Indication of any immediate medical attention and special treatment needed

No further relevant information available.

5 Fire-fighting measures

· Extinguishing media· Suitable extinguishing agents:

CO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.Use fire fighting measures that suit the environment.

· Advice for firefighters· Protective equipment: Wear protective clothing.

6 Accidental release measures

· Personal precautions, protective equipment and emergency procedures Wear protective clothing.· Environmental precautions: Dilute with plenty of water.· Methods and material for containment and cleaning up:

Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).· Reference to other sections

See Section 7 for information on safe handling.See Section 8 for information on personal protection equipment.See Section 13 for disposal information.

(Contd. on page 3)US

Page 43: 03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA ... R1060-1-50 RNA Lysis Buffer R1060-2-10

Page 3/8

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Trade name: DNase/RNase Free Water

(Contd. of page 2)

46.0

· Protective Action Criteria for Chemicals· PAC-1:

Substance is not listed.

· PAC-2:Substance is not listed.

· PAC-3:Substance is not listed.

7 Handling and storage

· Handling:· Precautions for safe handling No special measures required.· Information about protection against explosions and fires: No special measures required.

· Conditions for safe storage, including any incompatibilities· Storage:· Requirements to be met by storerooms and receptacles: No special requirements.· Information about storage in one common storage facility: Not required.· Further information about storage conditions: None.· Specific end use(s) Laboratory reagent

8 Exposure controls/personal protection

· Additional information about design of technical systems: No further data; see item 7.

· Control parameters· Components with limit values that require monitoring at the workplace: Not required.· Additional information: The lists that were valid during the creation were used as basis.

· Exposure controls· Personal protective equipment:· General protective and hygienic measures:

The usual precautionary measures for handling chemicals should be followed.· Breathing equipment: Not required.· Protection of hands:

The glove material has to be impermeable and resistant to the product/ the substance/ the preparation.Due to missing tests no recommendation to the glove material can be given for the product/ the preparation/ thechemical mixture.Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation

· Material of glovesThe selection of the suitable gloves does not only depend on the material, but also on further marks of quality andvaries from manufacturer to manufacturer.

· Penetration time of glove materialThe exact break through time has to be found out by the manufacturer of the protective gloves and has to beobserved.

(Contd. on page 4)US

Page 44: 03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA ... R1060-1-50 RNA Lysis Buffer R1060-2-10

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Trade name: DNase/RNase Free Water

(Contd. of page 3)

46.0

· Eye protection: Goggles recommended during refilling.

9 Physical and chemical properties

· Information on basic physical and chemical properties· General Information· Appearance:

Form: LiquidColor: Clear

· Odor: Odorless· Odor threshold: Not determined.

· pH-value: Not determined.

· Change in conditionMelting point/Melting range: 0 °C (32 °F)Boiling point/Boiling range: 100 °C (212 °F)

· Flash point: Not applicable.

· Flammability (solid, gaseous): Not applicable.

· Ignition temperature:

Decomposition temperature: Not determined.

· Auto igniting: Not determined.

· Danger of explosion: Product does not present an explosion hazard.

· Explosion limits:Lower: Not ApplicableUpper: Not Applicable

· Vapor pressure at 20 °C (68 °F): 23 hPa (17.3 mm Hg)

· Density at 20 °C (68 °F): 1 g/cm³ (8.345 lbs/gal)· Relative density Not determined.· Vapor density Not determined.· Evaporation rate Not determined.

· Solubility in / Miscibility withWater: Fully miscible.

· Partition coefficient (n-octanol/water): Not determined.

· Viscosity:Dynamic at 20 °C (68 °F): 0.952 mPasKinematic: Not determined.Water: 100.0 %VOC content: 0.00 %

0.0 g/l / 0.00 lb/gl

Solids content: 0.0 %

(Contd. on page 5)US

Page 45: 03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA ... R1060-1-50 RNA Lysis Buffer R1060-2-10

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Trade name: DNase/RNase Free Water

(Contd. of page 4)

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· Other information No further relevant information available.

10 Stability and reactivity

· Reactivity No further relevant information available.· Chemical stability· Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.· Possibility of hazardous reactions No dangerous reactions known.· Conditions to avoid No further relevant information available.· Incompatible materials: No further relevant information available.· Hazardous decomposition products: No dangerous decomposition products known.

11 Toxicological information

· Information on toxicological effects· Acute toxicity:· Primary irritant effect:· on the skin: No irritant effect.· on the eye: No irritating effect.· Sensitization: No sensitizing effects known.· Additional toxicological information:

When used and handled according to specifications, the product does not have any harmful effects according toour experience and the information provided to us.The substance is not subject to classification.

· Carcinogenic categories· IARC (International Agency for Research on Cancer)

Substance is not listed.

· NTP (National Toxicology Program)Substance is not listed.

· OSHA-Ca (Occupational Safety & Health Administration)Substance is not listed.

12 Ecological information

· Toxicity· Aquatic toxicity: No further relevant information available.· Persistence and degradability No further relevant information available.· Behavior in environmental systems:· Bioaccumulative potential No further relevant information available.· Mobility in soil No further relevant information available.· Additional ecological information:· General notes: Generally not hazardous for water

(Contd. on page 6)US

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Trade name: DNase/RNase Free Water

(Contd. of page 5)

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· Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.· Other adverse effects No further relevant information available.

13 Disposal considerations

· Waste treatment methods· Recommendation: Smaller quantities can be disposed of with household waste.

· Uncleaned packagings:· Recommendation:

Dispose of container in acoordance with local/regional/national and international recommendations.· Recommended cleansing agent: Water, if necessary with cleansing agents.

14 Transport information

· UN-Number· DOT, ADN, IMDG, IATA not regulated

· UN proper shipping name· DOT, ADN, IMDG, IATA not regulated

· Transport hazard class(es)

· DOT, ADN, IMDG, IATA· Class not regulated

· Packing group· DOT, IMDG, IATA not regulated

· Environmental hazards: Not applicable.

· Special precautions for user Not applicable.

· Transport in bulk according to Annex II ofMARPOL73/78 and the IBC Code Not applicable.

· UN "Model Regulation": not regulated

15 Regulatory information

· Safety, health and environmental regulations/legislation specific for the substance or mixture· Sara· Section 355 (extremely hazardous substances):

Substance is not listed.(Contd. on page 7)

US

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Trade name: DNase/RNase Free Water

(Contd. of page 6)

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· Section 313 (Specific toxic chemical listings):Substance is not listed.

· TSCA (Toxic Substances Control Act):Substance is listed.

· Proposition 65· Chemicals known to cause cancer:

Substance is not listed.

· Chemicals known to cause reproductive toxicity for females:Substance is not listed.

· Chemicals known to cause reproductive toxicity for males:Substance is not listed.

· Chemicals known to cause developmental toxicity:Substance is not listed.

· Carcinogenic categories· EPA (Environmental Protection Agency)

Substance is not listed.

· TLV (Threshold Limit Value established by ACGIH)Substance is not listed.

· NIOSH-Ca (National Institute for Occupational Safety and Health)Substance is not listed.

· GHS label elements Void· Hazard pictograms Void· Signal word Void· Hazard statements Void· Chemical safety assessment: A Chemical Safety Assessment has not been carried out.

16 Other informationThis information is based on our present knowledge. However, this shall not constitute a guarantee for any specificproduct features and shall not establish a legally valid contractual relationship.

· Department issuing SDS:Zymo Research Corp.Safety Department17062 Murphy Ave.Irvine, CA 92614USAPhone: 1-949-679-1190 or 1-888-882-9682

· Contact: [email protected]· Date of preparation / last revision 03/14/2018 / -· Abbreviations and acronyms:

ADR: Accord européen sur le transport des marchandises dangereuses par Route (European Agreement concerning the International Carriageof Dangerous Goods by Road)

(Contd. on page 8)US

Page 48: 03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA ... R1060-1-50 RNA Lysis Buffer R1060-2-10

Page 8/8

Safety Data Sheetacc. to OSHA HCS

Printing date 03/14/2018 Reviewed on 12/16/2016

Trade name: DNase/RNase Free Water

(Contd. of page 7)

46.0

IMDG: International Maritime Code for Dangerous GoodsDOT: US Department of TransportationIATA: International Air Transport AssociationACGIH: American Conference of Governmental Industrial HygienistsEINECS: European Inventory of Existing Commercial Chemical SubstancesCAS: Chemical Abstracts Service (division of the American Chemical Society)NFPA: National Fire Protection Association (USA)HMIS: Hazardous Materials Identification System (USA)VOC: Volatile Organic Compounds (USA, EU)PBT: Persistent, Bioaccumulative and ToxicvPvB: very Persistent and very BioaccumulativeNIOSH: National Institute for Occupational SafetyOSHA: Occupational Safety & HealthTLV: Threshold Limit ValuePEL: Permissible Exposure LimitREL: Recommended Exposure Limit

US

Page 49: 03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA ... R1060-1-50 RNA Lysis Buffer R1060-2-10

Page 1/8

Safety Data Sheetacc. to OSHA HCS

Printing date 03/14/2018 Reviewed on 12/12/2017

46.0

1 Identification

· Product identifier

· Trade name: ZymoBIOMICS HRC Prep Solution

· Article number: D4300-7-30, D4300-7-3· Application of the substance / the mixture Laboratory Reagent

· Details of the supplier of the safety data sheet· Manufacturer/Supplier:

Zymo Research Corp.17062 Murphy Ave.Irvine, CA 92614USAPhone: 1-949-679-1190 or [email protected]

· Information department: Product safety department· Emergency telephone number:

During normal business hours (8 am to 5 pm Pacific Standard Time): +1 (949) 679 1190

2 Hazard(s) identification

· Classification of the substance or mixtureThe product is not classified according to the Globally Harmonized System (GHS).

· Label elements· GHS label elements Void· Hazard pictograms Void· Signal word Void· Hazard statements Void· Classification system:· NFPA ratings (scale 0 - 4)

00

0Health = 0Fire = 0Reactivity = 0

· HMIS-ratings (scale 0 - 4)

HEALTH

FIRE

REACTIVITY

0

0

0

Health = 0Fire = 0Reactivity = 0

· Other hazards· Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.

US

(Contd. on page 2)

Page 50: 03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA ... R1060-1-50 RNA Lysis Buffer R1060-2-10

Page 2/8

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Printing date 03/14/2018 Reviewed on 12/12/2017

Trade name: ZymoBIOMICS HRC Prep Solution

(Contd. of page 1)

46.0

3 Composition/information on ingredients

· Chemical characterization: Mixtures· Description: Mixture of the substances listed below with nonhazardous additions.

· Dangerous components: Void

4 First-aid measures

· Description of first aid measures· General information: No special measures required.· After inhalation: Supply fresh air; consult doctor in case of complaints.· After skin contact: Generally the product does not irritate the skin.· After eye contact: Rinse opened eye for several minutes under running water. Then consult a doctor.· After swallowing: Do not induce vomiting; immediately call for medical help.· Information for doctor:· Most important symptoms and effects, both acute and delayed No further relevant information available.· Indication of any immediate medical attention and special treatment needed

No further relevant information available.

5 Fire-fighting measures

· Extinguishing media· Suitable extinguishing agents:

CO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.Use fire fighting measures that suit the environment.

· Advice for firefighters· Protective equipment: Wear protective clothing.

6 Accidental release measures

· Personal precautions, protective equipment and emergency procedures Wear protective clothing.· Environmental precautions:

Dilute with plenty of water.Do not allow to enter sewers/ surface or ground water.

· Methods and material for containment and cleaning up:Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).

· Reference to other sectionsSee Section 7 for information on safe handling.See Section 8 for information on personal protection equipment.See Section 13 for disposal information.

· Protective Action Criteria for Chemicals· PAC-1:

None of the ingredients is listed.(Contd. on page 3)

US

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Page 3/8

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Printing date 03/14/2018 Reviewed on 12/12/2017

Trade name: ZymoBIOMICS HRC Prep Solution

(Contd. of page 2)

46.0

· PAC-2:None of the ingredients is listed.

· PAC-3:None of the ingredients is listed.

7 Handling and storage

· Handling:· Precautions for safe handling No special measures required.· Information about protection against explosions and fires: No special measures required.

· Conditions for safe storage, including any incompatibilities· Storage:· Requirements to be met by storerooms and receptacles: No special requirements.· Information about storage in one common storage facility: Not required.· Further information about storage conditions: None.· Specific end use(s) Laboratory reagent

8 Exposure controls/personal protection

· Additional information about design of technical systems: No further data; see item 7.

· Control parameters· Components with limit values that require monitoring at the workplace:

The product does not contain any relevant quantities of materials with critical values that have to be monitored atthe workplace.

· Additional information: The lists that were valid during the creation were used as basis.

· Exposure controls· Personal protective equipment:· General protective and hygienic measures:

The usual precautionary measures for handling chemicals should be followed.· Breathing equipment: Not required.· Protection of hands:

The glove material has to be impermeable and resistant to the product/ the substance/ the preparation.Due to missing tests no recommendation to the glove material can be given for the product/ the preparation/ thechemical mixture.Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation

· Material of glovesThe selection of the suitable gloves does not only depend on the material, but also on further marks of quality andvaries from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance ofthe glove material can not be calculated in advance and has therefore to be checked prior to the application.

· Penetration time of glove materialThe exact break through time has to be found out by the manufacturer of the protective gloves and has to beobserved.

(Contd. on page 4)US

Page 52: 03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA ... R1060-1-50 RNA Lysis Buffer R1060-2-10

Page 4/8

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Printing date 03/14/2018 Reviewed on 12/12/2017

Trade name: ZymoBIOMICS HRC Prep Solution

(Contd. of page 3)

46.0

· Eye protection: Goggles recommended during refilling.

9 Physical and chemical properties

· Information on basic physical and chemical properties· General Information· Appearance:

Form: LiquidColor: Clear

· Odor: Odorless· Odor threshold: Not determined.

· pH-value: Not determined.

· Change in conditionMelting point/Melting range: Undetermined.Boiling point/Boiling range: Undetermined.

· Flash point: Not applicable.

· Flammability (solid, gaseous): Not applicable.

· Ignition temperature:

Decomposition temperature: Not determined.

· Auto igniting: Product is not selfigniting.

· Danger of explosion: Product does not present an explosion hazard.

· Explosion limits:Lower: Not ApplicableUpper: Not Applicable

· Vapor pressure: Not determined.

· Density: Not determined.· Relative density Not determined.· Vapor density Not determined.· Evaporation rate Not determined.

· Solubility in / Miscibility withWater: Fully miscible.

· Partition coefficient (n-octanol/water): Not determined.

· Viscosity:Dynamic: Not determined.Kinematic: Not determined.

· Solvent content:VOC content: 0.00 %

0.0 g/l / 0.00 lb/gl

(Contd. on page 5)US

Page 53: 03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA ... R1060-1-50 RNA Lysis Buffer R1060-2-10

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Printing date 03/14/2018 Reviewed on 12/12/2017

Trade name: ZymoBIOMICS HRC Prep Solution

(Contd. of page 4)

46.0

Solids content: 2.0 %· Other information No further relevant information available.

10 Stability and reactivity

· Reactivity No further relevant information available.· Chemical stability· Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.· Possibility of hazardous reactions No dangerous reactions known.· Conditions to avoid No further relevant information available.· Incompatible materials: No further relevant information available.· Hazardous decomposition products: No dangerous decomposition products known.

11 Toxicological information

· Information on toxicological effects· Acute toxicity:· Primary irritant effect:· on the skin: No irritant effect.· on the eye: No irritating effect.· Sensitization: No sensitizing effects known.· Additional toxicological information:

The product is not subject to classification according to internally approved calculation methods for preparations:When used and handled according to specifications, the product does not have any harmful effects according toour experience and the information provided to us.

· Carcinogenic categories· IARC (International Agency for Research on Cancer)

None of the ingredients is listed.

· NTP (National Toxicology Program)None of the ingredients is listed.

· OSHA-Ca (Occupational Safety & Health Administration)None of the ingredients is listed.

12 Ecological information

· Toxicity· Aquatic toxicity: No further relevant information available.· Persistence and degradability No further relevant information available.· Behavior in environmental systems:· Bioaccumulative potential No further relevant information available.· Mobility in soil No further relevant information available.

(Contd. on page 6)US

Page 54: 03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA ... R1060-1-50 RNA Lysis Buffer R1060-2-10

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Printing date 03/14/2018 Reviewed on 12/12/2017

Trade name: ZymoBIOMICS HRC Prep Solution

(Contd. of page 5)

46.0

· Additional ecological information:· General notes:

Water hazard class 1 (Self-assessment): slightly hazardous for waterDo not allow undiluted product or large quantities of it to reach ground water, water course or sewage system.

· Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.· Other adverse effects No further relevant information available.

13 Disposal considerations

· Waste treatment methods· Recommendation: Smaller quantities can be disposed of with household waste.

· Uncleaned packagings:· Recommendation:

Dispose of container in acoordance with local/regional/national and international recommendations.· Recommended cleansing agent: Water, if necessary with cleansing agents.

14 Transport information

· UN-Number· DOT, ADN, IMDG, IATA not regulated

· UN proper shipping name· DOT, ADN, IMDG, IATA not regulated

· Transport hazard class(es)

· DOT, ADN, IMDG, IATA· Class not regulated

· Packing group· DOT, IMDG, IATA not regulated

· Environmental hazards: Not applicable.

· Special precautions for user Not applicable.

· Transport in bulk according to Annex II ofMARPOL73/78 and the IBC Code Not applicable.

· UN "Model Regulation": not regulated

US

(Contd. on page 7)

Page 55: 03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA ... R1060-1-50 RNA Lysis Buffer R1060-2-10

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Printing date 03/14/2018 Reviewed on 12/12/2017

Trade name: ZymoBIOMICS HRC Prep Solution

(Contd. of page 6)

46.0

15 Regulatory information

· Safety, health and environmental regulations/legislation specific for the substance or mixture· Sara· Section 355 (extremely hazardous substances):

None of the ingredients is listed.

· Section 313 (Specific toxic chemical listings):None of the ingredients is listed.

· TSCA (Toxic Substances Control Act):None of the ingredients is listed.

· Proposition 65· Chemicals known to cause cancer:

None of the ingredients is listed.

· Chemicals known to cause reproductive toxicity for females:None of the ingredients is listed.

· Chemicals known to cause reproductive toxicity for males:None of the ingredients is listed.

· Chemicals known to cause developmental toxicity:None of the ingredients is listed.

· Carcinogenic categories· EPA (Environmental Protection Agency)

None of the ingredients is listed.

· TLV (Threshold Limit Value established by ACGIH)None of the ingredients is listed.

· NIOSH-Ca (National Institute for Occupational Safety and Health)None of the ingredients is listed.

· GHS label elements Void· Hazard pictograms Void· Signal word Void· Hazard statements Void· Chemical safety assessment: A Chemical Safety Assessment has not been carried out.

16 Other informationThis information is based on our present knowledge. However, this shall not constitute a guarantee for any specificproduct features and shall not establish a legally valid contractual relationship.

· Department issuing SDS:Zymo Research Corp.Safety Department17062 Murphy Ave.Irvine, CA 92614USA

(Contd. on page 8)US

Page 56: 03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA ... R1060-1-50 RNA Lysis Buffer R1060-2-10

Page 8/8

Safety Data Sheetacc. to OSHA HCS

Printing date 03/14/2018 Reviewed on 12/12/2017

Trade name: ZymoBIOMICS HRC Prep Solution

(Contd. of page 7)

46.0

Phone: 1-949-679-1190 or 1-888-882-9682· Contact: [email protected]· Date of preparation / last revision 03/14/2018 / -· Abbreviations and acronyms:

ADR: Accord européen sur le transport des marchandises dangereuses par Route (European Agreement concerning the International Carriageof Dangerous Goods by Road)IMDG: International Maritime Code for Dangerous GoodsDOT: US Department of TransportationIATA: International Air Transport AssociationACGIH: American Conference of Governmental Industrial HygienistsEINECS: European Inventory of Existing Commercial Chemical SubstancesELINCS: European List of Notified Chemical SubstancesCAS: Chemical Abstracts Service (division of the American Chemical Society)NFPA: National Fire Protection Association (USA)HMIS: Hazardous Materials Identification System (USA)VOC: Volatile Organic Compounds (USA, EU)PBT: Persistent, Bioaccumulative and ToxicvPvB: very Persistent and very BioaccumulativeNIOSH: National Institute for Occupational SafetyOSHA: Occupational Safety & HealthTLV: Threshold Limit ValuePEL: Permissible Exposure LimitREL: Recommended Exposure Limit

US

Page 57: 03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA ... R1060-1-50 RNA Lysis Buffer R1060-2-10

Page 1/8

Safety Data Sheetacc. to OSHA HCS

Printing date 03/14/2018 Reviewed on 02/02/2016

46.0

1 Identification

· Product identifier

· Trade name: DNase I

· Article number: E1009-A, E1011-A· Application of the substance / the mixture Laboratory Reagent

· Details of the supplier of the safety data sheet· Manufacturer/Supplier:

Zymo Research Corp.17062 Murphy Ave.Irvine, CA 92614USAPhone: 1-949-679-1190 or [email protected]

· Information department: Product safety department· Emergency telephone number:

During normal business hours (8 am to 5 pm Pacific Standard Time): +1 (949) 679 1190

2 Hazard(s) identification

· Classification of the substance or mixtureThe product is not classified according to the Globally Harmonized System (GHS).

· Label elements· GHS label elements Void· Hazard pictograms Void· Signal word Void· Hazard statements Void· Classification system:· NFPA ratings (scale 0 - 4)

00

0Health = 0Fire = 0Reactivity = 0

· HMIS-ratings (scale 0 - 4)

HEALTH

FIRE

REACTIVITY

0

0

0

Health = 0Fire = 0Reactivity = 0

· Other hazards· Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.

US

(Contd. on page 2)

Page 58: 03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA ... R1060-1-50 RNA Lysis Buffer R1060-2-10

Page 2/8

Safety Data Sheetacc. to OSHA HCS

Printing date 03/14/2018 Reviewed on 02/02/2016

Trade name: DNase I

(Contd. of page 1)

46.0

3 Composition/information on ingredients

· Chemical characterization: Mixtures· Description: Mixture of the substances listed below with nonhazardous additions.

· Dangerous components: Void

4 First-aid measures

· Description of first aid measures· General information: No special measures required.· After inhalation: Supply fresh air; consult doctor in case of complaints.· After skin contact: Generally the product does not irritate the skin.· After eye contact: Rinse opened eye for several minutes under running water. Then consult a doctor.· After swallowing: Do not induce vomiting; immediately call for medical help.· Information for doctor:· Most important symptoms and effects, both acute and delayed No further relevant information available.· Indication of any immediate medical attention and special treatment needed

No further relevant information available.

5 Fire-fighting measures

· Extinguishing media· Suitable extinguishing agents:

CO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.· Advice for firefighters· Protective equipment: Wear protective clothing.

6 Accidental release measures

· Personal precautions, protective equipment and emergency procedures Wear protective clothing.· Environmental precautions: Dilute with plenty of water.· Methods and material for containment and cleaning up:

Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).· Reference to other sections

See Section 7 for information on safe handling.See Section 8 for information on personal protection equipment.See Section 13 for disposal information.

· Protective Action Criteria for Chemicals· PAC-1:

None of the ingredients is listed.

· PAC-2:None of the ingredients is listed.

(Contd. on page 3)US

Page 59: 03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA ... R1060-1-50 RNA Lysis Buffer R1060-2-10

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Trade name: DNase I

(Contd. of page 2)

46.0

· PAC-3:None of the ingredients is listed.

7 Handling and storage

· Handling:· Precautions for safe handling No special measures required.· Information about protection against explosions and fires: No special measures required.

· Conditions for safe storage, including any incompatibilities· Storage:· Requirements to be met by storerooms and receptacles: No special requirements.· Information about storage in one common storage facility: Not required.· Further information about storage conditions: None.· Specific end use(s) No further relevant information available.

8 Exposure controls/personal protection

· Additional information about design of technical systems: No further data; see item 7.

· Control parameters· Components with limit values that require monitoring at the workplace:

The product does not contain any relevant quantities of materials with critical values that have to be monitored atthe workplace.

· Additional information: The lists that were valid during the creation were used as basis.

· Exposure controls· Personal protective equipment:· General protective and hygienic measures:

The usual precautionary measures for handling chemicals should be followed.· Breathing equipment: Not required.· Protection of hands:

The glove material has to be impermeable and resistant to the product/ the substance/ the preparation.Due to missing tests no recommendation to the glove material can be given for the product/ the preparation/ thechemical mixture.Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation

· Material of glovesThe selection of the suitable gloves does not only depend on the material, but also on further marks of quality andvaries from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance ofthe glove material can not be calculated in advance and has therefore to be checked prior to the application.

· Penetration time of glove materialThe exact break through time has to be found out by the manufacturer of the protective gloves and has to beobserved.

· Eye protection: Goggles recommended during refilling.US

(Contd. on page 4)

Page 60: 03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA ... R1060-1-50 RNA Lysis Buffer R1060-2-10

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Printing date 03/14/2018 Reviewed on 02/02/2016

Trade name: DNase I

(Contd. of page 3)

46.0

9 Physical and chemical properties

· Information on basic physical and chemical properties· General Information· Appearance:

Form: SolidColor: White

· Odor: Odorless· Odor threshold: Not determined.

· pH-value: Not determined.

· Change in conditionMelting point/Melting range: Undetermined.Boiling point/Boiling range: Undetermined.

· Flash point: Not applicable.

· Flammability (solid, gaseous): Not applicable.

· Ignition temperature:

Decomposition temperature: Not determined.

· Auto igniting: Product is not selfigniting.

· Danger of explosion: Product does not present an explosion hazard.

· Explosion limits:Lower: Not ApplicableUpper: Not Applicable

· Vapor pressure: Not determined.

· Density: Not determined.· Relative density Not determined.· Vapor density Not determined.· Evaporation rate Not determined.

· Solubility in / Miscibility withWater: Fully miscible.

· Partition coefficient (n-octanol/water): Not determined.

· Viscosity:Dynamic: Not determined.Kinematic: Not determined.

· Solvent content:Organic solvents: 0.0 %VOC content: 0.0 g/l / 0.00 lb/gl

· Other information No further relevant information available.

US

(Contd. on page 5)

Page 61: 03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA ... R1060-1-50 RNA Lysis Buffer R1060-2-10

Page 5/8

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Printing date 03/14/2018 Reviewed on 02/02/2016

Trade name: DNase I

(Contd. of page 4)

46.0

10 Stability and reactivity

· Reactivity No further relevant information available.· Chemical stability· Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.· Possibility of hazardous reactions No dangerous reactions known.· Conditions to avoid No further relevant information available.· Incompatible materials: No further relevant information available.· Hazardous decomposition products: No dangerous decomposition products known.

11 Toxicological information

· Information on toxicological effects· Acute toxicity:· Primary irritant effect:· on the skin: No irritant effect.· on the eye: No irritating effect.· Sensitization: No sensitizing effects known.· Additional toxicological information:

The product is not subject to classification according to internally approved calculation methods for preparations:When used and handled according to specifications, the product does not have any harmful effects according toour experience and the information provided to us.

· Carcinogenic categories· IARC (International Agency for Research on Cancer)

None of the ingredients is listed.

· NTP (National Toxicology Program)None of the ingredients is listed.

· OSHA-Ca (Occupational Safety & Health Administration)None of the ingredients is listed.

12 Ecological information

· Toxicity· Aquatic toxicity: No further relevant information available.· Persistence and degradability No further relevant information available.· Behavior in environmental systems:· Bioaccumulative potential No further relevant information available.· Mobility in soil No further relevant information available.· Additional ecological information:· General notes: Not known to be hazardous to water.· Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.

(Contd. on page 6)US

Page 62: 03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA ... R1060-1-50 RNA Lysis Buffer R1060-2-10

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(Contd. of page 5)

46.0

· Other adverse effects No further relevant information available.

13 Disposal considerations

· Waste treatment methods· Recommendation: Smaller quantities can be disposed of with household waste.

· Uncleaned packagings:· Recommendation:

Dispose of container in acoordance with local/regional/national and international recommendations.· Recommended cleansing agent: Water, if necessary with cleansing agents.

14 Transport information

· UN-Number· DOT, ADN, IMDG, IATA not regulated

· UN proper shipping name· DOT, ADN, IMDG, IATA not regulated

· Transport hazard class(es)

· DOT, ADN, IMDG, IATA· Class not regulated

· Packing group· DOT, IMDG, IATA not regulated

· Environmental hazards: Not applicable.

· Special precautions for user Not applicable.

· Transport in bulk according to Annex II ofMARPOL73/78 and the IBC Code Not applicable.

· UN "Model Regulation": not regulated

15 Regulatory information

· Safety, health and environmental regulations/legislation specific for the substance or mixture· Sara· Section 355 (extremely hazardous substances):

None of the ingredients is listed.

· Section 313 (Specific toxic chemical listings):None of the ingredients is listed.

· TSCA (Toxic Substances Control Act):None of the ingredients is listed.

(Contd. on page 7)US

Page 63: 03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA ... R1060-1-50 RNA Lysis Buffer R1060-2-10

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Trade name: DNase I

(Contd. of page 6)

46.0

· Proposition 65· Chemicals known to cause cancer:

None of the ingredients is listed.

· Chemicals known to cause reproductive toxicity for females:None of the ingredients is listed.

· Chemicals known to cause reproductive toxicity for males:None of the ingredients is listed.

· Chemicals known to cause developmental toxicity:None of the ingredients is listed.

· Carcinogenic categories· EPA (Environmental Protection Agency)

None of the ingredients is listed.

· TLV (Threshold Limit Value established by ACGIH)None of the ingredients is listed.

· NIOSH-Ca (National Institute for Occupational Safety and Health)None of the ingredients is listed.

· GHS label elements Void· Hazard pictograms Void· Signal word Void· Hazard statements Void· Chemical safety assessment: A Chemical Safety Assessment has not been carried out.

16 Other informationThis information is based on our present knowledge. However, this shall not constitute a guarantee for any specificproduct features and shall not establish a legally valid contractual relationship.

· Department issuing SDS:Zymo Research Corp.Safety Department17062 Murphy Ave.Irvine, CA 92614USAPhone: 1-949-679-1190 or 1-888-882-9682

· Contact: [email protected]· Date of preparation / last revision 03/14/2018 / -· Abbreviations and acronyms:

ADR: Accord européen sur le transport des marchandises dangereuses par Route (European Agreement concerning the International Carriageof Dangerous Goods by Road)IMDG: International Maritime Code for Dangerous GoodsDOT: US Department of TransportationIATA: International Air Transport AssociationEINECS: European Inventory of Existing Commercial Chemical SubstancesELINCS: European List of Notified Chemical SubstancesCAS: Chemical Abstracts Service (division of the American Chemical Society)PBT: Persistent, Bioaccumulative and Toxic

(Contd. on page 8)US

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Page 8/8

Safety Data Sheetacc. to OSHA HCS

Printing date 03/14/2018 Reviewed on 02/02/2016

Trade name: DNase I

(Contd. of page 7)

46.0

vPvB: very Persistent and very BioaccumulativeTLV: Threshold Limit ValuePEL: Permissible Exposure LimitREL: Recommended Exposure Limit

US

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Page 1/8

Safety Data Sheetacc. to OSHA HCS

Printing date 03/14/2018 Reviewed on 02/03/2016

46.0

1 Identification

· Product identifier

· Trade name: DNA Digestion Buffer

· Article number: E1010-1-4, E1010-1-16

· Details of the supplier of the safety data sheet· Manufacturer/Supplier:

Zymo Research Corp.17062 Murphy Ave.Irvine, CA 92614USAPhone: 1-949-679-1190 or [email protected]

· Information department: Product safety department· Emergency telephone number:

During normal business hours (8 am to 5 pm Pacific Standard Time): +1 (949) 679 1190

2 Hazard(s) identification

· Classification of the substance or mixtureThe product is not classified according to the Globally Harmonized System (GHS).

· Label elements· GHS label elements Void· Hazard pictograms Void· Signal word Void· Hazard statements Void· Classification system:· NFPA ratings (scale 0 - 4)

00

0Health = 0Fire = 0Reactivity = 0

· HMIS-ratings (scale 0 - 4)

HEALTH

FIRE

REACTIVITY

0

0

0

Health = 0Fire = 0Reactivity = 0

· Other hazards· Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.

3 Composition/information on ingredients

· Chemical characterization: Mixtures· Description: Mixture of the substances listed below with nonhazardous additions.

(Contd. on page 2)US

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(Contd. of page 1)

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· Dangerous components: Void

4 First-aid measures

· Description of first aid measures· General information: No special measures required.· After inhalation: Supply fresh air; consult doctor in case of complaints.· After skin contact: Generally the product does not irritate the skin.· After eye contact: Rinse opened eye for several minutes under running water. Then consult a doctor.· After swallowing: Do not induce vomiting; immediately call for medical help.· Information for doctor:· Most important symptoms and effects, both acute and delayed No further relevant information available.· Indication of any immediate medical attention and special treatment needed

No further relevant information available.

5 Fire-fighting measures

· Extinguishing media· Suitable extinguishing agents:

CO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.· Advice for firefighters· Protective equipment: Wear protective clothing.

6 Accidental release measures

· Personal precautions, protective equipment and emergency procedures Wear protective clothing.· Environmental precautions: Dilute with plenty of water.· Methods and material for containment and cleaning up:

Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).· Reference to other sections

See Section 7 for information on safe handling.See Section 8 for information on personal protection equipment.See Section 13 for disposal information.

· Protective Action Criteria for Chemicals· PAC-1:

None of the ingredients is listed.

· PAC-2:None of the ingredients is listed.

· PAC-3:None of the ingredients is listed.

US

(Contd. on page 3)

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Trade name: DNA Digestion Buffer

(Contd. of page 2)

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7 Handling and storage

· Handling:· Precautions for safe handling No special measures required.· Information about protection against explosions and fires: No special measures required.

· Conditions for safe storage, including any incompatibilities· Storage:· Requirements to be met by storerooms and receptacles: No special requirements.· Information about storage in one common storage facility: Not required.· Further information about storage conditions: None.· Specific end use(s) No further relevant information available.

8 Exposure controls/personal protection

· Additional information about design of technical systems: No further data; see item 7.

· Control parameters· Components with limit values that require monitoring at the workplace:

The product does not contain any relevant quantities of materials with critical values that have to be monitored atthe workplace.

· Additional information: The lists that were valid during the creation were used as basis.

· Exposure controls· Personal protective equipment:· General protective and hygienic measures:

The usual precautionary measures for handling chemicals should be followed.· Breathing equipment: Not required.· Protection of hands:

The glove material has to be impermeable and resistant to the product/ the substance/ the preparation.Due to missing tests no recommendation to the glove material can be given for the product/ the preparation/ thechemical mixture.Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation

· Material of glovesThe selection of the suitable gloves does not only depend on the material, but also on further marks of quality andvaries from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance ofthe glove material can not be calculated in advance and has therefore to be checked prior to the application.

· Penetration time of glove materialThe exact break through time has to be found out by the manufacturer of the protective gloves and has to beobserved.

· Eye protection: Goggles recommended during refilling.US

(Contd. on page 4)

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Trade name: DNA Digestion Buffer

(Contd. of page 3)

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9 Physical and chemical properties

· Information on basic physical and chemical properties· General Information· Appearance:

Form: LiquidColor: Colorless

· Odor: Odorless· Odor threshold: Not determined.

· pH-value: Not determined.

· Change in conditionMelting point/Melting range: Undetermined.Boiling point/Boiling range: Undetermined.

· Flash point: Not applicable.

· Flammability (solid, gaseous): Not applicable.

· Ignition temperature:

Decomposition temperature: Not determined.

· Auto igniting: Product is not selfigniting.

· Danger of explosion: Product does not present an explosion hazard.

· Explosion limits:Lower: Not ApplicableUpper: Not Applicable

· Vapor pressure: Not determined.

· Density: Not determined.· Relative density Not determined.· Vapor density Not determined.· Evaporation rate Not determined.

· Solubility in / Miscibility withWater: Fully miscible.

· Partition coefficient (n-octanol/water): Not determined.

· Viscosity:Dynamic: Not determined.Kinematic: Not determined.

· Solvent content:Organic solvents: 0.0 %VOC content: 0.0 g/l / 0.00 lb/gl

· Other information No further relevant information available.

US

(Contd. on page 5)

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Trade name: DNA Digestion Buffer

(Contd. of page 4)

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10 Stability and reactivity

· Reactivity No further relevant information available.· Chemical stability· Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.· Possibility of hazardous reactions No dangerous reactions known.· Conditions to avoid No further relevant information available.· Incompatible materials: No further relevant information available.· Hazardous decomposition products: No dangerous decomposition products known.

11 Toxicological information

· Information on toxicological effects· Acute toxicity:· Primary irritant effect:· on the skin: No irritant effect.· on the eye: No irritating effect.· Sensitization: No sensitizing effects known.· Additional toxicological information:

The product is not subject to classification according to internally approved calculation methods for preparations:When used and handled according to specifications, the product does not have any harmful effects according toour experience and the information provided to us.

· Carcinogenic categories· IARC (International Agency for Research on Cancer)

None of the ingredients is listed.

· NTP (National Toxicology Program)None of the ingredients is listed.

· OSHA-Ca (Occupational Safety & Health Administration)None of the ingredients is listed.

12 Ecological information

· Toxicity· Aquatic toxicity: No further relevant information available.· Persistence and degradability No further relevant information available.· Behavior in environmental systems:· Bioaccumulative potential No further relevant information available.· Mobility in soil No further relevant information available.· Additional ecological information:· General notes: Generally not hazardous for water· Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.

(Contd. on page 6)US

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(Contd. of page 5)

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· Other adverse effects No further relevant information available.

13 Disposal considerations

· Waste treatment methods· Recommendation: Smaller quantities can be disposed of with household waste.

· Uncleaned packagings:· Recommendation:

Dispose of container in acoordance with local/regional/national and international recommendations.· Recommended cleansing agent: Water, if necessary with cleansing agents.

14 Transport information

· UN-Number· DOT, ADN, IMDG, IATA not regulated

· UN proper shipping name· DOT, ADN, IMDG, IATA not regulated

· Transport hazard class(es)

· DOT, ADN, IMDG, IATA· Class not regulated

· Packing group· DOT, IMDG, IATA not regulated

· Environmental hazards: Not applicable.

· Special precautions for user Not applicable.

· Transport in bulk according to Annex II ofMARPOL73/78 and the IBC Code Not applicable.

· UN "Model Regulation": not regulated

15 Regulatory information

· Safety, health and environmental regulations/legislation specific for the substance or mixture· Sara· Section 355 (extremely hazardous substances):

None of the ingredients is listed.

· Section 313 (Specific toxic chemical listings):None of the ingredients is listed.

· TSCA (Toxic Substances Control Act):None of the ingredients is listed.

(Contd. on page 7)US

Page 71: 03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA ... R1060-1-50 RNA Lysis Buffer R1060-2-10

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Trade name: DNA Digestion Buffer

(Contd. of page 6)

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· Proposition 65· Chemicals known to cause cancer:

None of the ingredients is listed.

· Chemicals known to cause reproductive toxicity for females:None of the ingredients is listed.

· Chemicals known to cause reproductive toxicity for males:None of the ingredients is listed.

· Chemicals known to cause developmental toxicity:None of the ingredients is listed.

· Carcinogenic categories· EPA (Environmental Protection Agency)

None of the ingredients is listed.

· TLV (Threshold Limit Value established by ACGIH)None of the ingredients is listed.

· NIOSH-Ca (National Institute for Occupational Safety and Health)None of the ingredients is listed.

· GHS label elements Void· Hazard pictograms Void· Signal word Void· Hazard statements Void· Chemical safety assessment: A Chemical Safety Assessment has not been carried out.

16 Other informationThis information is based on our present knowledge. However, this shall not constitute a guarantee for any specificproduct features and shall not establish a legally valid contractual relationship.

· Department issuing SDS:Zymo Research Corp.Safety Department17062 Murphy Ave.Irvine, CA 92614USAPhone: 1-949-679-1190 or 1-888-882-9682

· Contact: [email protected]· Date of preparation / last revision 03/14/2018 / -· Abbreviations and acronyms:

ADR: Accord européen sur le transport des marchandises dangereuses par Route (European Agreement concerning the International Carriageof Dangerous Goods by Road)IMDG: International Maritime Code for Dangerous GoodsDOT: US Department of TransportationIATA: International Air Transport AssociationEINECS: European Inventory of Existing Commercial Chemical SubstancesELINCS: European List of Notified Chemical SubstancesCAS: Chemical Abstracts Service (division of the American Chemical Society)PBT: Persistent, Bioaccumulative and Toxic

(Contd. on page 8)US

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(Contd. of page 7)

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vPvB: very Persistent and very BioaccumulativeTLV: Threshold Limit ValuePEL: Permissible Exposure LimitREL: Recommended Exposure Limit

US

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46.0

1 Identification

· Product identifier

· Trade name: Zymo-Spin III-HRC Filters

· Article number: C1058-50, C1058-5· Application of the substance / the mixture Laboratory Reagent

· Details of the supplier of the safety data sheet· Manufacturer/Supplier:

Zymo Research Corp.17062 Murphy Ave.Irvine, CA 92614USAPhone: 1-949-679-1190 or [email protected]

· Information department: Product safety department· Emergency telephone number:

During normal business hours (8 am to 5 pm Pacific Standard Time): +1 (949) 679 1190

2 Hazard(s) identification

· Classification of the substance or mixtureThe product is not classified according to the Globally Harmonized System (GHS).

· Label elements· GHS label elements Void· Hazard pictograms Void· Signal word Void· Hazard statements Void· Classification system:· NFPA ratings (scale 0 - 4)

00

0Health = 0Fire = 0Reactivity = 0

· HMIS-ratings (scale 0 - 4)

HEALTH

FIRE

REACTIVITY

0

0

0

Health = 0Fire = 0Reactivity = 0

· Other hazards· Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.

US

(Contd. on page 2)

Page 74: 03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA ... R1060-1-50 RNA Lysis Buffer R1060-2-10

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(Contd. of page 1)

46.0

3 Composition/information on ingredients

· Chemical characterization: Mixtures· Description: Mixture of the substances listed below with nonhazardous additions.

· Dangerous components: Void

4 First-aid measures

· Description of first aid measures· General information: No special measures required.· After inhalation: Supply fresh air; consult doctor in case of complaints.· After skin contact: Generally the product does not irritate the skin.· After eye contact: Rinse opened eye for several minutes under running water. Then consult a doctor.· After swallowing: Do not induce vomiting; immediately call for medical help.· Information for doctor:· Most important symptoms and effects, both acute and delayed No further relevant information available.· Indication of any immediate medical attention and special treatment needed

No further relevant information available.

5 Fire-fighting measures

· Extinguishing media· Suitable extinguishing agents:

CO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.Use fire fighting measures that suit the environment.

· Special hazards arising from the substance or mixture Nitrogen oxides (NOx)· Advice for firefighters· Protective equipment: Wear protective clothing.

6 Accidental release measures

· Personal precautions, protective equipment and emergency procedures Wear protective clothing.· Environmental precautions: Do not allow to enter sewers/ surface or ground water.· Methods and material for containment and cleaning up:

Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).· Reference to other sections

See Section 7 for information on safe handling.See Section 8 for information on personal protection equipment.See Section 13 for disposal information.

· Protective Action Criteria for Chemicals· PAC-1:

None of the ingredients is listed.(Contd. on page 3)

US

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(Contd. of page 2)

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· PAC-2:None of the ingredients is listed.

· PAC-3:None of the ingredients is listed.

7 Handling and storage

· Handling:· Precautions for safe handling No special measures required.· Information about protection against explosions and fires: No special measures required.

· Conditions for safe storage, including any incompatibilities· Storage:· Requirements to be met by storerooms and receptacles: No special requirements.· Information about storage in one common storage facility: Not required.· Further information about storage conditions: None.· Specific end use(s) Laboratory reagent

8 Exposure controls/personal protection

· Additional information about design of technical systems: No further data; see item 7.

· Control parameters· Components with limit values that require monitoring at the workplace:

The product does not contain any relevant quantities of materials with critical values that have to be monitored atthe workplace.

· Additional information: The lists that were valid during the creation were used as basis.

· Exposure controls· Personal protective equipment:· General protective and hygienic measures:

The usual precautionary measures for handling chemicals should be followed.· Breathing equipment: Not required.· Protection of hands:

The glove material has to be impermeable and resistant to the product/ the substance/ the preparation.Due to missing tests no recommendation to the glove material can be given for the product/ the preparation/ thechemical mixture.Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation

· Material of glovesThe selection of the suitable gloves does not only depend on the material, but also on further marks of quality andvaries from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance ofthe glove material can not be calculated in advance and has therefore to be checked prior to the application.

· Penetration time of glove materialThe exact break through time has to be found out by the manufacturer of the protective gloves and has to beobserved.

(Contd. on page 4)US

Page 76: 03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA ... R1060-1-50 RNA Lysis Buffer R1060-2-10

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(Contd. of page 3)

46.0

· Eye protection: Goggles recommended during refilling.

9 Physical and chemical properties

· Information on basic physical and chemical properties· General Information· Appearance:

Form: Solid materialColor: Whitish

· Odor: No information available· Odor threshold: Not determined.

· pH-value: Not determined.

· Change in conditionMelting point/Melting range: Undetermined.Boiling point/Boiling range: Undetermined.

· Flash point: Not applicable.

· Flammability (solid, gaseous): Not applicable.

· Ignition temperature:

Decomposition temperature: Not determined.

· Auto igniting: Product is not selfigniting.

· Danger of explosion: Product does not present an explosion hazard.

· Explosion limits:Lower: Not ApplicableUpper: Not Applicable

· Vapor pressure: Not determined.

· Density: Not determined.· Relative density Not determined.· Vapor density Not determined.· Evaporation rate Not determined.

· Solubility in / Miscibility withWater: Not miscible or difficult to mix.

· Partition coefficient (n-octanol/water): Not determined.

· Viscosity:Dynamic: Not determined.Kinematic: Not determined.

· Solvent content:VOC content: 0.00 %

0.0 g/l / 0.00 lb/gl

(Contd. on page 5)US

Page 77: 03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA ... R1060-1-50 RNA Lysis Buffer R1060-2-10

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(Contd. of page 4)

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Solids content: 0.0 %· Other information No further relevant information available.

10 Stability and reactivity

· Reactivity No further relevant information available.· Chemical stability· Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.· Possibility of hazardous reactions No dangerous reactions known.· Conditions to avoid No further relevant information available.· Incompatible materials: No further relevant information available.· Hazardous decomposition products: No dangerous decomposition products known.

11 Toxicological information

· Information on toxicological effects· Acute toxicity:· Primary irritant effect:· on the skin: No irritant effect.· on the eye: No irritating effect.· Sensitization: No sensitizing effects known.· Additional toxicological information:

The product is not subject to classification according to internally approved calculation methods for preparations:When used and handled according to specifications, the product does not have any harmful effects according toour experience and the information provided to us.

· Carcinogenic categories· IARC (International Agency for Research on Cancer)

Trade Secret 001-1090 3

· NTP (National Toxicology Program)None of the ingredients is listed.

· OSHA-Ca (Occupational Safety & Health Administration)None of the ingredients is listed.

12 Ecological information

· Toxicity· Aquatic toxicity: No further relevant information available.· Persistence and degradability No further relevant information available.· Behavior in environmental systems:· Bioaccumulative potential No further relevant information available.· Mobility in soil No further relevant information available.

(Contd. on page 6)US

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(Contd. of page 5)

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· Additional ecological information:· General notes:

Water hazard class 1 (Self-assessment): slightly hazardous for waterDo not allow undiluted product or large quantities of it to reach ground water, water course or sewage system.

· Results of PBT and vPvB assessment· PBT: Not applicable.· vPvB: Not applicable.· Other adverse effects No further relevant information available.

13 Disposal considerations

· Waste treatment methods· Recommendation: Smaller quantities can be disposed of with household waste.

· Uncleaned packagings:· Recommendation:

Dispose of container in acoordance with local/regional/national and international recommendations.

14 Transport information

· UN-Number· DOT, ADN, IMDG, IATA not regulated

· UN proper shipping name· DOT, ADN, IMDG, IATA not regulated

· Transport hazard class(es)

· DOT, ADN, IMDG, IATA· Class not regulated

· Packing group· DOT, IMDG, IATA not regulated

· Environmental hazards: Not applicable.

· Special precautions for user Not applicable.

· Transport in bulk according to Annex II ofMARPOL73/78 and the IBC Code Not applicable.

· UN "Model Regulation": not regulated

US

(Contd. on page 7)

Page 79: 03/14/2018 Kit Components Product code Description R2001 … · 2019-02-19 · R1100-50 DNA/RNA Shield - 50 ml, 250 ml & sample; DNA/RNA ... R1060-1-50 RNA Lysis Buffer R1060-2-10

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Trade name: Zymo-Spin III-HRC Filters

(Contd. of page 6)

46.0

15 Regulatory information

· Safety, health and environmental regulations/legislation specific for the substance or mixture· Sara· Section 355 (extremely hazardous substances):

None of the ingredients is listed.

· Section 313 (Specific toxic chemical listings):None of the ingredients is listed.

· TSCA (Toxic Substances Control Act):All ingredients are listed.

· TSCA new (21st Century Act) (Substances not listed)· Proposition 65· Chemicals known to cause cancer:

None of the ingredients is listed.

· Chemicals known to cause reproductive toxicity for females:None of the ingredients is listed.

· Chemicals known to cause reproductive toxicity for males:None of the ingredients is listed.

· Chemicals known to cause developmental toxicity:None of the ingredients is listed.

· Carcinogenic categories· EPA (Environmental Protection Agency)

None of the ingredients is listed.

· TLV (Threshold Limit Value established by ACGIH)None of the ingredients is listed.

· NIOSH-Ca (National Institute for Occupational Safety and Health)None of the ingredients is listed.

· GHS label elements Void· Hazard pictograms Void· Signal word Void· Hazard statements Void· Chemical safety assessment: A Chemical Safety Assessment has not been carried out.

16 Other informationThis information is based on our present knowledge. However, this shall not constitute a guarantee for any specificproduct features and shall not establish a legally valid contractual relationship.

· Department issuing SDS:Zymo Research Corp.Safety Department17062 Murphy Ave.Irvine, CA 92614

(Contd. on page 8)US

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Printing date 03/14/2018 Reviewed on 11/20/2017

Trade name: Zymo-Spin III-HRC Filters

(Contd. of page 7)

46.0

USAPhone: 1-949-679-1190 or 1-888-882-9682

· Contact: [email protected]· Date of preparation / last revision 03/14/2018 / -· Abbreviations and acronyms:

ADR: Accord européen sur le transport des marchandises dangereuses par Route (European Agreement concerning the International Carriageof Dangerous Goods by Road)IMDG: International Maritime Code for Dangerous GoodsDOT: US Department of TransportationIATA: International Air Transport AssociationACGIH: American Conference of Governmental Industrial HygienistsEINECS: European Inventory of Existing Commercial Chemical SubstancesELINCS: European List of Notified Chemical SubstancesCAS: Chemical Abstracts Service (division of the American Chemical Society)NFPA: National Fire Protection Association (USA)HMIS: Hazardous Materials Identification System (USA)VOC: Volatile Organic Compounds (USA, EU)PBT: Persistent, Bioaccumulative and ToxicvPvB: very Persistent and very BioaccumulativeNIOSH: National Institute for Occupational SafetyOSHA: Occupational Safety & HealthTLV: Threshold Limit ValuePEL: Permissible Exposure LimitREL: Recommended Exposure Limit

US