-Ribograma - A New Method to Survey Cancer -

8/3/2019 -Ribograma - A New Method to Survey Cancer -

1/31

6

F eip gi~mcn ~c qyxviz afeaix

XJHC@XFGF

F EIP GI^MCNOCXNJF@ECQJQ+

^MIXF\IY^JAQ FENECE'JE_FQJ_I

QAXIIEJE@COAFEAIXNJQIFQI

\XCDIA^ OCX JG\KIGIE^F^JCE CO F\\KJAF^JCE JE ^MI NFJKZAKJEJAFK \XF^JAI

XJHC@XFGF4aceai|~ fen akjejafk f||kjaf~jceqje afeaix ~mixf|z%

FY^CX4 F% OIXXIJXF FKIGFC GN+ \mN-YEJ_IXQJNFNACG\KYÎEQINI GFNXJN*

FHQ^XFA^

^mi |yx|cqi co ~mjq xiqifxam+ pmjam jq pfq |xiqie~in fq f Nca~cxfk ^miqjq+ f~ YEJ_IXQJNFN

ACG\KYÎEQI NI GFNXJN+ pj~m ~mi fhcvi ~j~ki+ jq ~mi ockkcpje`4 ' Nxfo~ f gi~mcn co

njfècqjq+ qaxiieje` fen gcej~cxje` ~mi afeaix njqifqi ocyenin ce ~iamejafk fen ayxxie~aceai|~q co Gckiaykfx Hjckc`z% ^mjq eip gi~mcn fkkcpq f njfècqjq je f (|xi'afxajecgf je qj~yq~fì+ qjeai j~ jq hfqin je ~mi |miec~z|i co aikkq+ acg|cqin co vixz mj`m yfe~j~jiq co oxiixjhcqcgiq je j~q ~mi az~c|kfqg+ pmjam afe hi yfe~jojin hz gifeq co ~mi co ~mi okcp az~cgi~xz+fo~ix j~q jqckf~jce+ fen gfxbin pj~m okycxcamxcgiq% ^mi xi|if~in xiacxnq co ~mcqi yfe~j~jiq cooxii xjhcqcgiq+ iq~fhkjqmiq f `xf|mja ayxvi -XJHC@XFGF* ~mf~ xi|xiqie~q ~mi ni`xii co fgfkjèfe~ ~ienieaz+ pmjam fhcvi f aix~fje kivik co aceaie~xf~jce+ fkkcpq ~c qfz ~mf~ ~mi aikkq je f~jqqyi cx ackkia~jce co aikkq afe hi aceqjnixin je f |xcaiqq co nivikc|je` afeaix+ hiocxi fezgfaxcqac|ja cx gjaxcqac|ja vjipje`% ^mjq gifeq+ pi fxi ofaje` f eip gi~mcn+ ce f akifxhjcgckiaykfx kivik+ fq qmcpe je ~mi Nca~cxfk ^miqjq+ pj~m fnvfe~fìq -ocx `ccn |xc`xiqq* ce~mi gfaxcqac|z fen gjaxcqac|z% ^mjq gi~mcn i}aiinq ~mi qac|i co c~mix ece'jevfqjvi gi~mcnq%^mfebq ~c ~mjq gi~mcn+ j~ jq |cqqjhki ~c mfvi f qaxiieje` |xc`xfg je pmjam fkk aj~jtieq pmc|xiqie~ f jeaxifqin ayxvi coXJHC@XFGFgyq~ hi gfenf~cxz amiabin pj~m f njfècq~ja fen~mixf|iy~ja akjejafk ~cckq+ fen f~ ~mi qfgi ~jgi+ xigfje yenix chqixvf~jce fen ~mixf|z+ pj~m |xcvie ioojajieaz je ~mi |xivie~jce fen xinya~jce co ~mi |xigfkjèfe~ ~ienieaz jedyxz+xi|xiqie~je` `xif~ qfvje`q je ~mi ~mixf|iy~ja jevixqjce co afeaix%

JE^XCJ^


2/31

8

^mi XJHC@XFGFGI^MCN jq f xifk hjckc`jafk hjcgfxbix ~c gcej~cxjqi ~mi himfvjcyx#|miec~z|ico f acggyej~z co aikkq yenix q~ynz#~xif~gie~ -i% `% hxifq~+ ackcxia~fk ~xfa~+ y||ix njìq~jvi ~yhi+kye`+ |xcq~f~i+ `zeiackc`ja ~xfa~+ i~a*+ hiocxi gi~fq~f~ja afeaix i}|kcqjce%

Jo ~mjq hjcgfxbix jq fnc|~in+ pi afe fefkzti qcgicei'q ~ygcx ~jqqyi hiocxi ~xif~gie~ fen qiimcp ~miz xiq|cen ~c ginjaf~jce fen ockkcp ~mi xykiq co |ixqcefkjtin ~xif~gie~q%

^mi XJHC@XFGFGI^MCNjq f q~xce` ~cck co ~mi amigjq~xz+gfeyofa~yxje fenace~xck co~mi eip -fen#cx cx|mfe nxy`q*+ fq pikk ~mi ckn ginjajeiq+ hiafyqi j~ jq hfqin ce ~mi giamfejqgqco ~mi yejvixqfk hjckc`jafk gfaxcgckiaykiq je ~mi az~c|kfqg+ kjbi ~mi oxiixjhcqcgiq%

^mi XJHC@XFGFGI^MCN jq fe je vj~xc|xinja~jvihjc|mfxgfaiy~jafk~cck ocx nxy`~mixf|iy~jaq amcjai+ iq|iajfkkz je qieqjvj~z ~c afeaix aikk kjeiq fen ~c q~ig aikk ayk~yxiq -je vjip ~c~mi qieqjvj~z co aikk y|q~xifg gfamjeixz ~c ~mi oxiixjhcqcgiq*+ qjeai cei afe xifn ~mimj`meiqq co ~mi XJHC@XFGF ayxvi je oxce~ ~c f aix~fje fa~jce co f qyhq~feai%

^mi XJHC@XFGFGI^MCN mik|q ~mi yjabkz niajqjce je ~mi amcjai co ~mi hiq~ fe~j|xckjoixf~jvinxy` je afeaix ~mixf|z fen ~mi yenixq~fenje` co ~mi himfvjcyx co mygfe jenyain |kyxj|c~ie~

q~ig aikkq ~c ~mi nxy` fa~jce%

^mi `cfk coXJHC@XFGFjq fe jeecvf~jvi hjcgckiaykfx gi~mcn ocx vixz ifxkz njfècqjq -ec~jevfqjvi* co afeaix+ pmjam jq hfqin je f qjg|ki eip hjcgckiaykfx f||xcfam+ qjgjkfx ocx fkk ~z|iqco afeaix+ |fx~jaykfxkz migf~ckc`jafk afeaixq fen afeaixq fooia~je` ~mi kye`+ hxifq~+`fq~xcje~iq~jefk ~xfa~+ |feaxifq+ `zeiackc`z fen yxcìej~fk ~xfa~q%

J~ pjkk iemfeai ~mi kjoi co |f~jie~q fen jg|xcvi ~mi gfefìgie~ co afeaix njqifqi+ fkkcpje`gcej~cxje` ~mi ioojafaz co fez afeaix ~xif~gie~ fen c~mix eceafeaix njqifqiq%

Faacxnje` cyx |xiqie~ yenixq~fenje` co ~mi gyk~j|ki becpe hfqja giamfejqgq co ~mi yeace~xckkinaikk `xcp~m fen gi~fq~fqjq+ ~mixi jq f `xcpje` hj` eyghix co gyk~j|ki |f~mpfzq+ oxcg ~migfkjèfe~ ~ygcyx `xcp~m ~c fe`jcìeiqjq fen gi~fq~fqjq ~mf~ acg|kjaf~iq ~mi niqjè co f~xfeqvixqfk gi~mcn co njfècqjq co afeaix co iybfxzc~i aikkq%

^mixi jq f vixz pjni qcyxai co jeocxgf~jce ce aikk qjèfkje` |f~mpfzq -y| fen ncpeq~xifg je ~miaikk* pmjam gfbiq ~mi ~fkbq je ~mi aikkykfx hjcgckiaykfx |xcaiqqiq acg|ki} fen njoojayk~ ~c xifn~mi `kchfk ace~i}~+ qyam fq je qjèfk ~xfeqnya~jce xiqcyxaiq+ jeocxgf~jce ce Fb~ qjèfkje`+f|c|~cqjq+ afeaix |f~mpfzq+ afq|fqi afqafniq fen |f~mpfzq+ ~mi aikk azaki+ aikk nif~m |f~mpfz+aikk q~xiqq+ azakja eyakic~jniq+ az~cqbiki~ce+ i}~xfaikkykfx gf~xj} qjèfkje`+ @ |xc~ijeq+ ìeixi`ykf~jce+ `xcp~m ofa~cxq+ mcxgceiq+ jggyei aikk qjèfkje`+ jce amfeeikq+ kj|jnq+ gyk~jnxy`xiqjq~feai+ eiyxchjckc`z+ eiyxc~xfeqgjqqjce+ ej~xja c}jni+ |mcq|mcxzkf~jce+ xiai|~cx qjèfkje` fenxiai|~cx qjèfk |f~mpfzq+ qiacenfxz giqqieìxq fen qjèfkje` gckiaykiq%

^mi eyghix+ ~mi axcqq~fkb fen acg|ki}j~z co ~mi qjèfkje` pfzq je ~mi oyea~jce co ~mi aikkykfxhjcgckiaykfx ei~pcxb ef~yxfkkz hiacgi fe chq~faki ~c niqjè f ~xfeqvixqfk gi~mcn ~c njfècqi fmz|ix|xckjoixf~jvi q~f~i co f acggyej~z co aikkq%

Pj~m ~mi XJHC@XFGF GI^MCN j~ jq |cqqjhki ~c niqjè f ayxvi co yfe~j~jiq co oxiixjhcqqcgfq je ~mi az~c|kfqg co f acggyej~z co aikkq yenix q~ynz+ fkkcpje` je fnvfeai ~migcej~cxje` co j~q hjcgckiaykfx xiq|ceqi+ je f `kchfk fen je~i`xf~in ofqmjce+ fkkcpje` ~c becp jopi fxi je f q~f~i co mz|ix|xckjoixf~jce cx je f njgjejqmje` co ~mi q~f~i co |xckjoixf~jce%


3/31

1

Nioia~q je aikkqjèfkje` |f~mpfzq fkkcp afeaix aikkq ~c fk~ix ~mijx ecxgfk |xc`xfggiq co|xckjoixf~jce+ ~xfeqaxj|~jce+ `xcp~m+ gj`xf~jce+ njooixie~jf~jce fen nif~m% ^ygcyx ocxgf~jce fen|xc`xiqqjce caayx ~mxcy`m f xfeì co nioia~q ~mf~ nivikc| hc~m pj~mje fen cy~qjni ~mi afeaix aikk%Gfez njooixie~ fe~jafeaix fìe~q mfvi ~mixiocxi hiie nivikc|in ~c ~fxì~ |xc~ijeq ~mf~ fa~ jegfez co ~miqi |f~mpfzq% ^miqi vixz jg|cx~fe~ fq|ia~q fhcy~ ~mi aikk himfvjcx afel~ `jvi fgcej~cxje` yfe~j~f~jvi jeocxgf~jce kjbi ~mi XJHC@XFGF%

F \IXQ\IA^J_ICE^MIHJCKC@ZCOGFKJ@EFEÂIKKQ

Qivixfk kjeiq co ivjnieai+ qcgi pikk iq~fhkjqmin fen xiaie~+ qfz ig|mf~jafkkz ~mi jnif ~mf~ XEF ace~ie~ jq mj`m jeafeaix aikkq fen ìei~ja ivie~q ~mf~ kifn ~c afeaix fxi co~ie kjebin+ njxia~kz cx jenjxia~kz+ ~c ~mi x jhcqcgi hjcìeiqjq-60=%60:*%

Ce ~mi c~mix mfen+ ~mi jevixqi |xchkig jq amiabin je ~mi afqiq co (jnjc|f~mja jeiooia~jvi feigjf - JJI Jeiooia~jviixz~mxc|cjiqjq jnjc|f~mja*+ pmixi ~mixi fxi jeqyoojajie~ xf~iq co aikk |xckjoixf~jce+ fen |f~jie~q mfvi ixz~mxchkfq~q

ace~fjeje` cekz 0


4/31

>

ocxg f q~fenfxn ayxvi# fvixfì eyghix co xjhcqcgiq |ix yej~ vckygi -XJHC@XFGF* je xikf~jce ~c ~mi aikkq kjeje`~mi ackce fen xia~yg+ jqckf~in fen qi|fxf~in oxcg ~mi oiaiq%

Kjbi gfez ojiknq co kjoi qajieaiq+ hjckc`z co afeaix jq fe i}|ceie~jfkkz `xcpje` ojikn fen mj`mkz acg|ki}+ jeakynje`pcxb ~mf~ mfq f xfeì oxcg gckiaykfx hjckc`z ~c i|jnigjckc`z ceacìeiq ievjxcegie~% Qyxvjvfk xf~iq ocx vfxjcyq

afeaixq+ ceai akjejafkkz gfejoiq~+ mfvi qmcpe gcniq~ jg|xcvigie~ je xiaie~ niafniq%

^mixi jq f q~xce` gc~jvf~jce ~c je~i`xf~i njooixie~ ojiknq co becpkinì je afeaix hjckc`z fen je~xcnyai f eipaceai|~yfk fen ~micxi~jafk oxfgipcxb ~mf~ afe jg|xcvi ~mi yenixq~fenje` co ~ygcx nzefgjaq xiqifxamixq qc+ ~c

nivikc| hi~~ix ~mixf|iy~ja gifqyxiq% J~ pjkk hi jg|cx~fe~ ~mi |xcnya~jce co giamfejq~jafkkz |xinja~jvi gcnikq hfqince ~ygcx nzefgjaq ~mf~ afe fhq~xfa~ ~mi gifeje` co ~mi xikf~jvikz fnvfeain hjckc`jafk ni~fjkq ~c ceacìeiqjq fen~ygcx |xc`xiqqjce% Pmjki j~ jq ~xyi ~mf~ afeaix jq f gyk~jofai~in njqifqi pj~m f vfxji~z co akcqi (~xj`ìxq( je njooixie~~jqqyiq fen njooixie~ |f~jie~q+ ~mixi jq fkqc f q~xce` |cqqjhjkj~z ~mf~ afeaixq qmfxi f aie~xfk oif~yxi cxj`jef~je` oxcg facggce aikkykfx gfamjeixz pmjam ~mi aikkq ni|ien ocx ~mijx |xckjoixf~jce -600*%

^mi gcq~ vjqjhki fq|ia~ co f``xiqqjvi gfkjèfeajiq jq jeaxifqin aikk |xckjoixf~jce+ pmjam mfq f~ j~q acxi f gfxbinjeaxifqi je |xc~ije qze~miqjq% Je ~mi |xcaiqq co ecxgfk gj~cìeja xiq|ceqi+ ~mixi jq f ~xfeqjie~ fen azakjafk jeaxifqije ~mi xf~i co ìeixfk |xc~ije qze~miqjq% ^mi cvixfkk jeaxifqi je |xc~ije qze~miqjq jq f eiaiqqfxz |miecgiecechqixvin ace~xckkin hiocxi aikk njvjqjce+ kifnje` ~c ny|kjaf~jce co ace~ie~ fen ~mi jeaxifqi je qjti hiocxi ~mi ecxgfkgj~cqjq% ^myq+ ~mi fvixfì qjti co ~mi aikkq jq gfje~fjein nyxje` |mzqjckc`jafk |xckjoixf~jvi xiq|ceqi%

Cei co ~mi biz giamfejqgq co kcqq co ace~xck co |xc~ije qze~miqjq je ~xfeqocxgin aikkq jq ~mi jefhjkj~z ~c niaxifqi ~mieyghix co xjhcqcgiq ~mf~ jq acxxikf~in pj~m aikk |xckjoixf~jce je oxiqm ayk~yxi ginjf pj~mcy~ fnnin `xcp~m ofa~cxq ~cqixyg -69?*% Gfez xiqifxamixq mfvi chqixvin je ~jqqyi ayk~yxiq+ njooixieaiq je `xcp~m |xc|ix~jiq hi~piie ecxgfkaikkq fen ~mijx gfkjèfe~ acye~ix|fx~q+ cei co pmjam |cje~q ~c ~mi ofjkyxi co ~miqi ~c qmcp f azakja vfxjf~jce co aikkykfxfen hjcamigjafk |fxfgi~ixq ~mxcy`m ~mi aikk azaki cx `xcp~m azaki -69:'6?8*% \xivjcyqkz+ c~mix xiqifxamixq mfvi`jvie f~~ie~jce ~c amfeìq ~mf~ caayx je ~mi xf~i co |xc~ije qze~miqjq fen oyea~jce co gfamjeiq (~xfeqkf~jcefk( co ~miaikk+ xikf~jvi ~c ~mi aikk azaki+ ceai qyam amfeìq fxi eiaiqqfxz ~xfeqj~jceq `xcp~m fen gyk~j|kjaf~jce je ecxgfkacenj~jceq%

^mieyakickyq

Je ~mjq qieqi+ j~ gfbiq qieqi ~c ~fkb fhcy~ ~mi ofa~ ~mf~ gfez |f~mckc`jq~q nyxje` ~mi xcy~jei i}fgjef~jce co hjc|qzgf~ixjfk ivfkyf~jce+ co~ie |fz f~~ie~jce ~c ~mi eyakickj -~mi qj~iq pmixi xXEF jq qze~miqjtin fen xjhcqcgiq fxi(fqqighkin(* + fq je gfkjèfe~ eic|kfqgq+ eyakickj fxi fk~ixin je qcgi pfz xikf~jvi ~c ~mi eyghix fen qjti% ^myq+

~mi fqqcajf~jce co eyakickj fk~ixin je eic|kfq~ja njq~yxhfeaiq mfq hiie xioixxin ~c xiqifxam oxcg gcxi ~mfe ojo~z zifxqf`c -690*% ^mi eyakickyq jq f oyea~jcefk+ pikkniojein q~xya~yxfk yej~ je ~mi je~ix|mfqi aikk je pmjam xjhcqcgfk ìeiqfxi kcaf~in fen pmixi xjhcqcgfk XEF qze~miqjq -6?1* caayxq% Fgce` c~mix oyea~jceq+ ~mi eyakickyq+ pmjam jq f bizcx`feikki+ accxnjef~iq ~mi qze~miqjq fen fqqighkz co xjhcqcgfk yej~q%

^mi |xcnya~jce co xjhcqcgiq jq fe jg|cx~fe~ gi~fhckja fa~jvj~z fen ~myq ~mi xcki co ~mi eyakickyq jq akcqikz kjebin ~caikk `xcp~m fen xiaie~ nf~f qy`ìq~ ~mf~ ~mi eyakickyq fkqc |kfzq fe jg|cx~fe~ xcki je aikk azaki xi`ykf~jce+

qieiqaieai fen q~xiqq xiq|ceqiq -6==*%

^mi xjhcqcgi hjcìeiqjq jevckviq ~mi qze~miqjq co xXEF+ gf~yxf~jce fen fqqighkz co XEF fen xjhcqcgfk |xc~ijeq jexjhcqcgfk qyhyej~q+ qgfkk fen kfxì% ^mjq |xcaiqq jq xi`ykf~in ~mxcy`m ~mi aikk azaki+ |xjgfxjkz f~ ~mi kivik co xXEFqze~miqjq -6=:*% XNEF ~xfeqaxj|~jce xifamiq j~q |ifb nyxje` ~mi Q |mfqi fen @8+ jq je~ixxy|~in pmie ~mi aikk ie~ixq

gj~cqjq fen xifa~jvf~in pmie aikkq i}j~ gj~cqjq -6:*% ^mi ~pc gcq~ jg|cx~fe~ |xc~ijeq eyakickje fen |xc~ije H81 fxi jevckvinje xXEF qze~miqjq fen |xcaiqqje`% -6?9*%

Ce q~ynjiq co ~ygcx hjc|qz oxf`gie~q ocx je qj~y chqixvf~jce az~cmjq~ckc`jafk fen jqckf~in |xc~ijeq+ j~ jq vixjojin ~mf~~mi fgcye~ co FÈCX |xc~ijeq jeaxifqiq |xc`xiqqjvikz pmie xiq~je` aikkq ie~ix ~mi gj~c~ja azaki oxcg @6 ~c ~mi ienco Q |mfqi -6?? ' 6?=*% Fkqc+ ~mixi jq ivjnieai ~mf~ ~mi fgcye~ co FÈCX |xc~ijeq jq njxia~kz xikf~in ~c ~mi xf|jnj~z coaikk |xckjoixf~jce je aikk kjeiq iq~fhkjqmin mygfe afeaix -6?:'606*%


5/31

9

^mi ECX -Eyakickfx Cx`fejqix xi`jce* fxi qj~iq co xXEF ~xfeqaxj|~jce+ gcnjojaf~jce |cq~~xfeqaj|ajcefk co XEF~xfeqaxj|~q fen j~q (fqqighkz( je oyea~jcefk xjhcqcgiq% ^mi eyghix co ECXq i}|xiqqin je f ~jqqyi jq xikf~in ~c ~mixf~i co aikk |xckjoixf~jce+ pj~m njooixie~jf~jce fen eic|kfq~ja ~xfeqocxgf~jce% ^mjq mfq hiie yqin ~c nigceq~xf~i ~mi|c~ie~jfk gfkjèfeaz fen ~c fqqiqq |xcècqjq fen gfkjèfe~ f``xiqqjce -601'609*%

Vyjiqaie~ migf~c|cji~ja aikkq qyam fq ~mi ec~ fa~jvf~in ^ fen H kzg|mcaz~iq+ fen |kyxj|c~ie~ q~ig aikkq+ fxi qgfkk

pj~m kcp ace~ie~ co |xc~ije fen XEF ~mfe ~mijx acye~ix|fx~q je ~mi q~f~i co |xckjoixf~jce%N

yxje` ~mi ~xfeqj~jce oxcg @


6/31

?

^cnfz cei afeec~ pfj~ ocx ~mi qjèq fen qzg|~cgq+ hy~ ziq cei gyq~ `c ~c gii~ ~mi fnvie~ co ~mi afyqiqco afeaix% ^mi q~xf~i`z jq ec~ ~c i}|ia~+ hy~ |xc`xiqq ce ~mi pfz ~c gii~ fen acghf~ ~mi afyqiq fen ojen ~mi afeaixje f ifxkz |mfqi co mifkje`% Ivixz~mje` gyq~ hi afq~ je ~mi (|xi'je qj~y(+ pmjam jq ~mi hiq~ ~jgi ~c q|ifb ~c ayxiafeaix%

^mi AXA mfq f kce` |xigfkjèfe~ |mfqi fen f qkcp |xc`xiqqjce oxcg ~mi q~fì co njqifqi aceojein ~c ~mipfkk co ~mi cx`fe ~c ~mi q~fìq co kcafk jevfqjce fen njq~fe~ gi~fq~f~ja njqifqi% ^mixiocxi+ ~mixi jq fg|ki c||cx~yej~z

~c jnie~joz |f~jie~q f~ f ayxfhki q~fì pj~m ~mi acg|ki~jce co kfxì qaxiieje`q% Ayxxie~kz+ gi~mcnq co f||xcfam fxi ec~zi~ q|iajoja+ qyam fq ~mi jeviq~j`f~jce co caayk~ hkccn je q~cck+ cx ~mcqi ~mf~ xikz ce jevfqjvi ~iamejyiq ocx njxia~vjqyfkjtf~jce+ qyam fq oki}jhki qj`gcjncqac|z+ ackcecqac|z cx hfxjyg ieigf% Xiaie~ fnvfeaiq je akjejafk amigjq~xzfen gckiaykfx hjckc`z kin ~c ~mi igixìeai co eip ~iamejyiq ocx ecejevfqjvi f||xcfam+ hfqin ce ~mi hjckc`zyenixkzje` AXA% ^mjq f||xcfam jnie~jojin gy~f~jceq ~mf~ fxi becpe ~c hi fqqcajf~in pj~m AXA je ~mi NEF ~mf~ jqqmin je oiaiq ocx gfkjèfe~ fen |xigfkj efe~ kiqjceq co ackce%

Niq|j~i gfez q~ynjiq cvix ~mi |fq~ 6< zifxq+ ~mi jg|fa~ co gfez nji~fxz ofa~cxq je ackcxia~fkafxajecìeiqjq jq q~jkk ec~ xiqckvin% Ackcxia~fk afeaix ~xienq je ~mi Yej~in Q~f~iq fxi qjèjojafe~kz njooixie~ oxcg ~mcqico Iyxc|i% Je ~mi Yej~in Q~f~iq+ ~mi jeajnieai mfq jeaxifqin qkj`m~kz qjeai 6:06+ pmjki je kf~i 6::< gcx~fkj~z mfqniakjein fhcy~ 9


7/31

0

^mi nivikc|gie~ co ecejevfqjvi ~iq~q+ nixjvin oxcg becpkinì co hfqja ~ygcx hjckc`z+ qmcpq ~mi |xcgjqi cojg|xcvin ~fxì~in hfxxjixq ~c ackcecqac|z+ fe i}fgjef~jce co qaxiieje` fen njfècqjq coAXA% Pi fxi fpfxi comygfe NEF gy~f~jceq je q~cck+ pmjam fxi fqqcajf~in pj~m ~mi AXA fen |xigfkjèfe~ ackcxia~fk |ckz|q%

^mi becpkinì co ~mjq ofa~ mfq kin ~c ~mi f||ifxfeai co f hfqja gi~mcnckc`z -AXA qaxiieje`* q~ynzje` gy~f~jceq je~mi NEF co aikkq qmin oxcg ~mi ackcxia~fk gyacqf% ^mi qajieai himjen ~mi q~ynz coNEF gy~f~jceq jq qc|mjq~jaf~in%Pmie eic|kfqgq fxi nivikc|in+ aikkq fen oxf`gie~q fxi qafkz fen xikifqin je ~mi oiaiq je ~mi je~iq~jefk kygie+

kifnje` ~mi q~cckN

EF acg|xjqje` ~mi qfg|ki ocx ~iq~je`%

^mi ~iq~je` co gy~f~jceq fen oiaiq mfvi ofkqi |cqj~jvi fen ofkqi ei`f~jvi+ pj~m qivixfk kfxì fg|kj~yni+ qfz ~mify~mcxq% Gy~f~jceq je ~mi ~xjfkq fen oiaiq gfz hikce` ~c c~mix cx`fe - xiq|jxf~cxz ~xii+ `fq~xcje~iq~jefk ~xfa~+ y|q~xifgco ackcxia~+ i~a*+ ec~ fkkcpje` ~mi fqqyxfeai co ~mi kcaf~jce co |cqqjhki ~ygcxq je ~mi fkjgie~fxz ~xfa~% Je fnnj~jce+gy~f~jceq je ~mi ~iq~q -gy~f~jceq coNEF je oiaiq* gfz ec~ hi gy~fe~ |miec~z|iq+ pmjam ace~xjhy~i ~c ~mi `xif~|ixaie~fìq co ofkqi |cqj~jviq%

JJ ' \XCAINYXIQ OCX \XI\FXF^JCE

FENACYE^JE@ OXII XJHCQCGIQ

ÎAMEJAFK \MFQIQ OCX ACEAXI^JTF^JCE CO ^MIXJHC@XFGF GI^MCN

Ocx ~mi acye~je` cooxiixjhcqcgiq je ~mi yej~ vckygi yenix q~ynz pjkk ig|kcz ~mi ockkcpje` ~iamejyiq fen#cx|mfqiq4


8/31

=

^mioj`yxiqmcpqfei}fg|kicofqamigije pmjam~mif}iq+ pmjamxi|xiqie~~mi~pcafx~iqjfeaccxnjef~iq% Ceiaceq~xya~qfayxvi~cxi|xiqie~~miamfeìco~mi yfe~j~zcooxiixjhcqcgiq|ixaikk-cx|ixyej~ vckygi*jefniojein~jgi|ixjcn+ pj~mjefefvixfìco~mii}ockjf~inaikkqcoackcxia~fkgyacqfcof|f~jie~%

JEÎX\XI^F^JCE CO ^MI XJHC@XFGF AYX_I

Ayxvi ~mf~ q~fx~q f~ ~mi ecxgfk kivik+ fen xifamje` ~mi kivik jeaxifqin+ pmjam

jevckviq afxi ocx f |xc|mzkfa~ja q~ynz ce ~mi xifqceq cx afyqiq ~mf~ ni~ixgjei j~q f||ifxfeai% ^mi aikkq f~ ~mjq kivik-jeaxifqin* q~jkk mfvi ec qiayxj~z oif~yxiq co gfkjèfeaz+ hy~ ~mijx jeaxifqin eyghix co x jhcqcgfq ace~jeyiq `xcpje`%^mi aikk jq ~xfeqocxgin je~c f gfkjèfe~ |miec~z|i -fkfxg kivik*% F~ ~mjq |cje~ j~ jq iqqie~jfk ~c gfbi f nji~fxz q~ynz ~cqyxviz fen jnie~joz |cqqjhki gy~f~jceq xiq|ceqjhki ocx ~mi yeace~xckkin `xcp~m co xjhcqcgi hjcìeiqjq je ~mi |xcaiqqco afxajecìeiqjq2

Qi`gie~ co ~mi ayxvi nixjvin oxcg ~mi fe~ixjcx -qi`gie~ f'h*+ pmjam

acxxiq|cenq ~c ~mi |miec~z|ja fk~ixf~jceq fen ~c ~mi vjqjhki kj`m~ gjaxcqac|z je pmjam aikkq |xiqie~ fkxifnz pj~m|miec~z|ja oif~yxiq co gfkjèfeaz2

Qi`gie~ co ~mi ayxvi+ ace~jeyje` ~mi fe~ixjcx qi`gie~+ pmjam xi|xiqie~q ~mi

kivik co qjèq fen qzg|~cgq co ~mi AXA njqifqi -akjejafk kivik*2^mjq qi`gie~ co ~mi ayxvi acxxiq|cenq ~c f amfeì je njxia~jce co ~mi ayxvi f'h

~mf~ xiqyk~q oxcg |xivie~jvi fa~jce fen ~xif~gie~ -ginjaf~jceq fenq|iajfk nji~*2

acxxiq|cenq ~c f ayxvi je pmjam aikkq `xcp -gyk~j|kz cx |xckjoixf~i* je f qikogcej~cxje` giamfejqgq+ jeaxifqin |xckjoixf~jce+ fq je ~mi afqi co ~mi qigjejoixcyq ~yhykiq+ cx ~mi iencgi~xjyg% ^mjqjeaxifqin kivik co xjhcqcgi hjcìeiqjq ~mi ace~xck ~mixico jq pj~mje ~mi |mzqjckc`jafk -ecegfkj efe~*2

Ecxgfk ayxvi2

^mjq pcxb jq je~ienin ~c q~ynz ~mi j~ig ackcxia~fk afeaix -AXA*+ je pmjam

xjhc`xfgf ayxvi jq mj`m+ qc j~ jq ec~ ~mi ~jgi ~c ~fkb fhcy~ ~mi gifeje` co j~ f~ kcp kivik% Q~jkk j~ pjkk hi yqioyk ~c q~ynzacenj~jceq co niìeixf~jce fen jevcky~jce co aikkq fen ~jqqyiq+ ij~mix ~mi je~iq~jefk gyacqf+ cx c~mix i}~xfje~iq~jefk~jqqyi%


9/31

:

J_ ' ^MIIQQIEAICO^MIGI^MCN

Je ~mi oxfgipcxb co ~mi gie~jcein oj`yxiq fxi niqjèin ~mi ~pc f}iq ~mf~ xi|xiqie~ ~mi ~pc afx~iqjfe accxnjef~iq-mcxjtce~fk f}jq fen vix~jafk f}jq* pj~m pmjam cei hyjk~ f `xf|mjafk ayxvi% ^mjq ayxvi jq amfxfa~ixjtin hz ~pc vfxjfhkiqfhcy~ ~mi oxii xjhcqcgiq co az~ckc`jafk acggyej~z co aikkq yenix q~ynz -~mi i|j~mikjfk aikkq co ackcxia~fk gyacqf cxackcecaz~i*% miqi vfxjfhkiq fxi4

(yfe~j~z -eyghix* fvixfì co xjhcqcgioxii je ~mi az~c|kfqg co ifam aikk fen

(~jgi( cvix pmjam jq chqixvin ~mi himfvjcx co aix~fje vfxjf~jceq co ~mi (yfe~j~z -eyghix* gife oxii xjhcqcgiq%

^mixi pjkk eiin ~c xfjqi i}|ixjgie~fkkz `xf|mja |xcojkiq co eygixjafk vfkyiq -gife yfe~j~z#eyghix co oxiixjhcqcgiq* ~mf~ afe hi qi~ fq ecxgfk+ oxcg pmjam cei afe nxfp f ayxvigjnnkixi`jq~ix% ^myq+ j~ jq |cqqjhki ~c gfbiacg|fxf~jvi q~ynjiq co kfxì |c|ykf~jce `xcy|q+ pmjam pjkk hi |cqqjhki ~c qii gyk~j|ki |c~ie~jfk ~xienq co gfkjèfe~njqifqi -hz xfjqje` ~mi ayxvi* je f acggyej~z co aikkq je f ~jqqyi yenix jeviq~j`f~jce%

Je q~ynjiq acxxikf~je` ikia~xce gjaxcqac|z fen hjcamigjafk nf~f -yqyfkkz yfe~j~f~jvi*+ ~mi gcx|mckc`jafk -ikia~xcegjaxcqac|z* jq gcxi kjgj~in fen ni|ienie~ co niqaxj|~jceq hfqin ce qyhdia~jvi axj~ixjf%

^mjq axj~ixjce -ikia~xce gjaxcqac|z f||xcfam* jq qyhdia~jvi hiafyqi j~ nciq ec~ fkkcp f q~f~jq~jafk gifqyxigie~ conf~f+ pj~m gf~migf~jafk xj`cx+ |xivie~je` f |fxfgi~xja acxxikf~jce pj~m gcx|mckc`jafk nf~f ocx hjcamigjafk nf~f%

Ie ik iqyigf ni kf oj`yxf mfz ye xi`jq~xc ni kf ivfkyfaje ayfe~j~f~jvf ni kcq xjhcqcgfq kjhxiq+ ie ik qie~jnciq~~jac z njegjac+ acgc jeocxgfaje xiac`jnf ni oxfaajceiq qyhaikykfxiq+ yi |yini qix acxxikfajcefnf ace c~xcq|fxgi~xcq ni gieqyxfaje hjck`jaf aikykfx ayfe~j~f~jvf -acgc |cx idig|kc+ fk`yecq gfxafncxiq ~ygcxfkiq+ i%`%AIF%*%

^mi cy~kjei co ~mi oj`yxi jq f xiacxn co ~mi yfe~j~f~jvi fqqiqqgie~ co oxii xjhcqcgiq je ~mi q~f~ja fen nzefgjaqieqi+ fq jeocxgf~jce ackkia~in oxcg qyhaikkykfx oxfa~jceq+ pmjam afe hi acxxikf~in pj~m c~mix |fxfgi~ixq coyfe~j~f~jvi aikk hjckc`jafk gifqyxigie~ -i`+ qcgi ~ygcx gfxbixq+ i` AIF%*%

F |fx~jx nik acecajgjie~c nik je~ixvfkc ni vfkcxiq afe~jnfn -egixc* ginjf ni xjhcqcgfq kjhxiq aceqjnixfncqnie~xc ni kf ecxgfkjnfn -je~ixvfkc F* qi |cnxe aceqjnixfx c~xcq je~ixvfkcq+ yi ie ik iqyigf ni kf oj`yxfiq~exi|xiqie~fncq |cxH ejvik fygie~fnc+ A ejvik ni fkfxgf+ N ejvik aejac+ F ejvik hfdc z < ejvik jei}jq~ie~i%

Oxcg ~mi becpkinì co ~mi xfeì co vfkyiq ( gife yfe~j~z -eyghix* co oxii xjhcqcgiq( aceqjnixin pj~mje ~miecxgfk xfeì -xfeì F* cei gfz aceqjnix c~mix je~ixvfkq je ~mi cy~kjei co ~mi oj`yxi fxi xi|xiqie~in hz H kivikjeaxifqin+ A kivik fkfxg+ N akjejafk kivik+ F 'kcp kivik fen < fhqie~%

Je ~mi mcxjtce~fk f}jq jq jeqix~in gyk~j|ki |ixjcnq co ~jgi+ ocx i}fg|ki+ ~mxii gce~mq+ -6+ 8+ 1+%%%%%%%%+6


10/31

6

ACKKIA^CX \FX^QHXIFBNCPE

Oj`yxi% 6

Oj`yxi% 8

Oj`yxiq 6'8 Niqaxj|~jce4Qy||cx~ q~fenfxn ackkia~cx#ace~fjeix'~c|'i}~ixjcx+ pmjam pjkk f||kz je ~mi ~cjki~

hcpk je ~mi ~cjki~ qif~ hije` acg|xiqqin -oj}in fen jefgchjhki* pmie ~mi|f~jie~'yqix+ oiik ocx ~mijx eiinq% ^mjq qy||cx~ mfq f qcabi~ pmixi ~mi y||ix xjgco ~mi ace~fjeix jq jggchjki+ fq qiie je Oj`yxiq 6'8%


25/31

89

Oj`yxi% 1

Oj`yxi% >

Oj`yxiq 1'> Niqaxj|~jce4Ace~fjeix#ackkia~cx'je~ixjcx co oiaiq+ pmjam jq ighinnin je ~mi |fx~ co Oj`yxiq6'8+ je pmjam qjni jq ~mi ace~fjeix#ackkia~cx'ojk~ixje` jenccx `xjkk kjbi f |jq~ce fq joj~ pixi f qzxje`i+ fq qmcpe je Oj`yxiq 9'?% ^mi ace~fjeix#ackkia~cx'je~ixjcx coOj`yxiq 1'> mfq f aie~xfk mcki je ~mi hc~~cg+ kjbi f azkjenxjafk oyeeik ~mf~ pjkk oj~je f ace~fjeix#ackkia~cx hikcp -Oj`yxiq 9'?*+ pmixi mi pjkk ackkia~ f pfqmje`

-qixyg f||xc|xjf~i ocx gfje~fjeje` vjfhjkj~z co aikkq je ~mi gyacqf ackcxia~fk#ackceja*% ^mjq q~cck jq pfqmje` ~mi jggixqjce co ~mi q~cck je ~mi qixyg-pmcqi amigjafk acg|cqj~jce jq i}|kfjein je ikqipmixi*+ pmjam pjkk okcp ~c ~miace~fjeix#ackkia~cx'hc~~cg fq qmcpe je Oj`yxiq 0'=+ fo~ix ~mi vfkvi ni|ja~in jeOj`yxiq :'6


26/31

8?

Oj`yxi 9

Oj`yxi ?

Oj`yxiq 9'? Niqaxj|~jce4Ace~fjeix#ackkia~cx'kiqq+ kccbje` azkjenxjafk pj~m fe c|ieje` je ~mi y||ix ofai+pj~m ~mi azkjenxjafk oyeeik ~mxcy`m pmjam ~c oj~+ kjbi f qzxjeì je ~mi c~mix oyeeik+jeqjni+ qc acf}jfk pj~m ~mi oyeeik f~ ~mi ~c|+ niqaxjhin je Oj`yxiq 1'>% ^mi AQI+fq ~mi azkjenix jq fndyq~in kcpix q~fì ce ~mi y||ix q~fì co ~miace~fjeix#ackkia~cx'kiqq

Ace~fjeix#kcpixackkia~cx


27/31

80

Oj`yxi 0

Oj`yxi =

Oj`yxiq 0'= Niqaxj|~jce4Acvix ocx qifkje` co ~mi ace~fjeix#af~amix'y||ix'cy~ix%


28/31

8=

Oj`yxi :

Oj`yxi 6


29/31

8:

Oj`yxi 66

Oj`yxi 68

Oj`yxiq 66'68 Niqaxj|~jce4_fkvi ~mf~ fkkcpq ~mi ~xfeqj~ yejnjxia~jcefk ace~fjeix#af~amix'y||ix'i}~ixjcx ~c~mi ace~fjeix#ackkia~cx'kiqq%


30/31

1

Oj`yxiq 61'6> Niqaxj|~jce4^xj'njgieqjcefk _jqjce -1N* co fkk q~cck ackkia~cx%


31/31

Kjqhce+ 6

-Ribograma - A New Method to Survey Cancer -

Documents

Transcript of -Ribograma - A New Method to Survey Cancer -