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![Page 1: Listed as endangered by USFWS in 1967 - part of first listed species under 1966 Endangered Species Preservation Act Comanche Springs Pupfish are currently.](https://reader038.fdocuments.us/reader038/viewer/2022110404/56649ebf5503460f94bc9abf/html5/thumbnails/1.jpg)
DFP project – Summer 2015An Investigation of Captive
Reproduction Methods in the Comanche Springs Pupfish
Cyprinodon elegans
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Background - Conservation Listed as endangered by USFWS in 1967
- part of first listed species under 1966 Endangered Species Preservation Act
Comanche Springs Pupfish are currently found only in 3 springs and 1 creek in TX:
Phantom Lake Spring, San Solomon Spring, Giffin Spring, and Toyah Creek near Balmorhea, Texas
Major Threats:- over pumping of
groundwater- habitat alterations- hybridization
![Page 3: Listed as endangered by USFWS in 1967 - part of first listed species under 1966 Endangered Species Preservation Act Comanche Springs Pupfish are currently.](https://reader038.fdocuments.us/reader038/viewer/2022110404/56649ebf5503460f94bc9abf/html5/thumbnails/3.jpg)
Background - Breeding Typically breed during warmer months (summer) Mating System: - Territorial (large ♂)
- Satellite (medium ♂) - Sneaker (small ♂)
Lay eggs singly, up to 30 per day in a territory Short lifespan - lives 1 to 2 years
♀ ♂
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DFP project Objectives
1. Experiment 1: Determine the success rates of pairwise mating of Comanche Springs pupfish in aquaria (e.g., 1M:1F)
2. Experiment 2: Determine if blocking sight to adjacent aquaria increases egg deposition
3. Experiment 3: Determine if 2 females paired with 1 male increases egg deposition
4. Estimate fecundity and gonadosomatic index (GSI)
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Methods
5 gallon aquaria, spawning tray w/ a spawning pad held in place by 2 rocks, air-stone, flow-through using a slow rate of input, visual barriers
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Results Overall (all experiments combined)
- 759 eggs deposited- 62 fry
Experiment 1: 1M:1F pairs- 102 eggs- 3 fry
Experiment 2: Blocked vision- 71 eggs- 0 fry
Experiment 3: 1M:1F vs 1M:2F- 586 eggs- 59 fry
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Comanche Springs Pupfish eggs (~1.5 mm)
Newly deposited eggs Eggs 48-hours after
fertilization
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Mycosis
Mycotic
infections in
~82% of eggs
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Comanche Springs Pupfish fry (~4 mm)
~ 7 days old close up
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Observations
Aggressive males # of mortalities - 48 females - 2 males
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Acknowledgements Thank you Uvalde NFH crew Thanks for feeding my fry Ruben ! Thank you so much to all who participated in
making the DFP program happen !! Thanks to all Region 2 staff for organizing this
gathering And thanks to the !
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Questions ?