Histology Tissues - animal, human, plant

Sources - medical, veterinary, research

Grossed, fixed, processed, embedded, sectioned, and stained

Important to the scientific investigation process To establish and confirm patient diagnosis

For data compilation for researchers.

What is histology?

Grossing Tissue is cut to appropriate

size & thickness

Fixation Stabilizes tissue proteins

Prevents self-destruction

What is histology?

Preserves cells/tissue constituents

SSHS offers a range of fixatives

Non-buffered formalin

Bouins’ solution

Zinc formalin

Processing Tissue runs overnight in a tissue processor

Tissue goes through series of reagents

Dehydrates tissue

Transition step

Fills with hot fluid wax

What is histology?

Embedding Tissue removed from processor cassette

Aligned in a mold

Molten paraffin poured over

Cooled to harden

What is histology?

Sectioning Use microtome

Cut into microscopically thin strips

Floated on a water bath to smooth out wrinkles

Sections arranged on microscope slides

What is histology?

Staining Slides are dried in front of a fan

Tissue is deparaffinized by heating in an oven

Slides are stained

Many different types of stains and staining techniques

Dependent on tissue type and what researcher/pathologist is looking for

What is histology?

Saffron will provide histology services tailored to

the unique needs of our research, veterinary,

physician, hospital, and surgery center clients.

The management team is known for high quality

slides, reliable service, fast turnaround, and

competitive prices.

Mission Statement