© 2015 BioLife Solutions, Inc. HypoThermosol ® Performance Evidence.
-
Upload
muriel-washington -
Category
Documents
-
view
223 -
download
0
description
Transcript of © 2015 BioLife Solutions, Inc. HypoThermosol ® Performance Evidence.
![Page 1: © 2015 BioLife Solutions, Inc. HypoThermosol ® Performance Evidence.](https://reader034.fdocuments.us/reader034/viewer/2022051201/5a4d1b657f8b9ab0599afae4/html5/thumbnails/1.jpg)
© 2015 BioLife Solutions, Inc.© 2015 BioLife Solutions, Inc.
HypoThermosol®
Performance Evidence
![Page 2: © 2015 BioLife Solutions, Inc. HypoThermosol ® Performance Evidence.](https://reader034.fdocuments.us/reader034/viewer/2022051201/5a4d1b657f8b9ab0599afae4/html5/thumbnails/2.jpg)
© 2015 BioLife Solutions, Inc.
5-Day Hypothermic Storage of Human DermalFibroblasts Enabled by HypoThermosol®
alamarBlue® stain for metabolic activity
* Contains serum
Assayed 24 Hours Post Storage - % Recovery of Viable Cells
DAY STORAGE1 DAY STORAGE3 DAY STORAGE5
![Page 3: © 2015 BioLife Solutions, Inc. HypoThermosol ® Performance Evidence.](https://reader034.fdocuments.us/reader034/viewer/2022051201/5a4d1b657f8b9ab0599afae4/html5/thumbnails/3.jpg)
© 2015 BioLife Solutions, Inc. 4
Representative Fluorescent Micrographs Illustrating Morphology of Normal Human Dermal Fibroblasts
Stored in HypoThermosol®
Green Actin CytoskeletonPhalloidin (FITC)
Red Mitochondria ActivationMitoTracker® Red
Blue Nuclear StainHoechst 40X magnification
Media with Serum
3 Day Storage
5 Day Storage
Celsior®
3 Day Storage
5 Day Storage
Viaspan®
3 Day Storage
5 Day Storage
HypoThermosol®
3 Day Storage
5 Day Storage
![Page 4: © 2015 BioLife Solutions, Inc. HypoThermosol ® Performance Evidence.](https://reader034.fdocuments.us/reader034/viewer/2022051201/5a4d1b657f8b9ab0599afae4/html5/thumbnails/4.jpg)
© 2015 BioLife Solutions, Inc. 6alamarBlue® stain for metabolic activity
* Contains serum
Extended Hypothermic Storage of Human MesenchymalStem Cells Enabled by HypoThermosol®
Assayed 24 Hours Post Storage - % Recovery of Viable Cells
DAY STORAGE1 DAY STORAGE3 DAY STORAGE5 DAY STORAGE7
![Page 5: © 2015 BioLife Solutions, Inc. HypoThermosol ® Performance Evidence.](https://reader034.fdocuments.us/reader034/viewer/2022051201/5a4d1b657f8b9ab0599afae4/html5/thumbnails/5.jpg)
© 2015 BioLife Solutions, Inc. 7
Representative Fluorescent Micrographs Illustrating Morphology of Human Mesenchymal Stem Cells Stored in HypoThermosol®
Green Actin CytoskeletonPhalloidin (FITC)
Red Mitochondria ActivationMitoTracker® Red
Blue Nuclear StainHoechst 20X magnification
Normosol®-R
3 Day Storage
5 Day Storage
Plasma®-Lyte
3 Day Storage
5 Day Storage
Viaspan®
3 Day Storage
5 Day Storage
HypoThermosol®
3 Day Storage
5 Day Storage
![Page 6: © 2015 BioLife Solutions, Inc. HypoThermosol ® Performance Evidence.](https://reader034.fdocuments.us/reader034/viewer/2022051201/5a4d1b657f8b9ab0599afae4/html5/thumbnails/6.jpg)
© 2015 BioLife Solutions, Inc. 9
Recovery of Human Osteoblast Cells Following Hypothermic Storage Assayed 24 Hours Post Storage
Differentiated Following 5-Day Hypothermic Storage of Undifferentiated hMSC in HypoThermosol® Assayed 24 Hours Post Storage - % Recovery of Viable Cells
DAY STORAGE1 DAY STORAGE3 DAY STORAGE6
alamarBlue® stain for metabolic activity
* Contains serum
![Page 7: © 2015 BioLife Solutions, Inc. HypoThermosol ® Performance Evidence.](https://reader034.fdocuments.us/reader034/viewer/2022051201/5a4d1b657f8b9ab0599afae4/html5/thumbnails/7.jpg)
© 2015 BioLife Solutions, Inc. 10
Representative Fluorescent Micrographs Illustrating Morphology of Human Osteoblast Cells Following Hypothermic Storage
20X magnification
Differentiated Following 5 Day Hypothermic Storage ofUndifferentiated hMSC in HypoThermosol
Green Actin CytoskeletonPhalloidin (FITC)
Red Mitochondria ActivationMitoTracker® Red
Blue Nuclear StainHoechst
Normosol®-R
3 Day Storage
6 Day Storage
Plasma®-Lyte
3 Day Storage
6 Day Storage
Viaspan®
3 Day Storage
6 Day Storage
HypoThermosol®
3 Day Storage
6 Day Storage
![Page 8: © 2015 BioLife Solutions, Inc. HypoThermosol ® Performance Evidence.](https://reader034.fdocuments.us/reader034/viewer/2022051201/5a4d1b657f8b9ab0599afae4/html5/thumbnails/8.jpg)
© 2015 BioLife Solutions, Inc. 12
24 Hour Post Storage Recovery of Human Dental PulpStem Cells Following Hypothermic Storage
Commercial Solution Comparison: Serum and Serum-Free Media
DAY STORAGE1 DAY STORAGE3 DAY STORAGE5
% Recovery of Viable Cells
alamarBlue® stain for metabolic activity
* Contains serum