© 2011 Fluxion Biosciences. All rights reserved. Brief timeline and flunding history.
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Transcript of © 2011 Fluxion Biosciences. All rights reserved. Brief timeline and flunding history.
![Page 1: © 2011 Fluxion Biosciences. All rights reserved. Brief timeline and flunding history.](https://reader035.fdocuments.us/reader035/viewer/2022062721/56649f265503460f94c3c8b9/html5/thumbnails/1.jpg)
© 2011 Fluxion Biosciences. All rights reserved.
Brief timeline and flunding history
![Page 2: © 2011 Fluxion Biosciences. All rights reserved. Brief timeline and flunding history.](https://reader035.fdocuments.us/reader035/viewer/2022062721/56649f265503460f94c3c8b9/html5/thumbnails/2.jpg)
Page 2© 2011 Fluxion Biosciences. All rights reserved.
Fluxion today
BioFlux 200 BioFlux 1000 IonFlux-16 IonFlux HT
• Company:– Venture backed since Dec 2007– 4 products commercialized and selling globally– Segment: Biotools– Over 100 units installed
• Products: instruments and consumables– BioFlux System for cell-cell interactions– IonFlux Automated Patch Clamp System– IsoFlux rare cell isolation platform – under development
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Page 3© 2011 Fluxion Biosciences. All rights reserved.
Partial customer list
Johnson & Johnson
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Page 4© 2011 Fluxion Biosciences. All rights reserved.© 2011 Fluxion Biosciences. All rights reserved.
IonFlux applications
• Compound profiling– Dose response measurements– Hit confirmation– Single concentration screening
• Mutant screening – Evaluate functional changes dependent on mutations– Record responses from up to 32 distinct mutants (8 for IF-HT)
• Structure-function studies– Determine the effect of mutations on channel function– Characterize expression for differentiated stem cells– Recording endogenously expressed ion channels– Screen clones based on ion channel density
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Page 5© 2011 Fluxion Biosciences. All rights reserved.© 2011 Fluxion Biosciences. All rights reserved.
IonFlux Technology for APC- Best in Class
• High quality data– G-seal quality recordings– Ensemble recording for improved
success rate
• Cost effective– Perform APC for about the cost of an
MPC setup
• Simple to use– Ease of use of a ‘plate reader’– Relevance of patch clamp data– Table top unit
• Reliable– No robotics required– Only two moving parts
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Page 6© 2011 Fluxion Biosciences. All rights reserved.
Ensemble vs. single cell recording
• Ensembles average signals over 20 patched cells (16 ensembles per plate, left panel)
• The new single cell consumable records from one cell per channel (16 cells per plate for IF-16, right panel)
• The single cell consumable utilizes the same hardware
20-cell ensemble Single cell
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Page 7© 2011 Fluxion Biosciences. All rights reserved.© 2010 Fluxion Biosciences. All rights reserved.
Well-Plate Integrated Microfluidics
Well-plate integrated microfluidics (WPM) utilizes standard 96- and 384-well plates, but the bottom of the plate is replaced with a microfluidic network interconnected between the wells.
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Page 8© 2011 Fluxion Biosciences. All rights reserved.
* Sample customer slide – Pfizer UK
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Page 9© 2011 Fluxion Biosciences. All rights reserved.© 2011 Fluxion Biosciences. All rights reserved.
High Level Timeline
Founded
2006 2007 2008 2009 2010 2011
Grant 1 Ph2
Grant 1 Ph1
Grant 2 Ph2
Grant 2 Ph1
BioFlux product launch
IonFlux product launch
Series A Series B
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Page 10© 2011 Fluxion Biosciences. All rights reserved.© 2011 Fluxion Biosciences. All rights reserved.
Grant pipeline and success rates
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Page 11© 2011 Fluxion Biosciences. All rights reserved.
SBIR: the good, the bad and the ugly
• The good:– Non-dilutive capital!– Can be the catalyst for new projects, encourages innovation– Early $$, which are the hardest
• The bad:– Long timelines: 9 mo. from application to funding– <200k is low capital for Phase I awards– Can be a distraction from the central mission
• The ugly:– Review process dependent on random events– In our case, projects with the greatest commercial worth didn’t get funded