Animal Shared Resource (ASR)

ZebrafishEric Glasgow, PhD eg239@georgetown.edu687-7350

ASR Zebrafish Services

• Fish Housing and Care

• Embryo Production

• Genotyping

• Manipulation of Gene Expression

- Morpholino, RNA, and DNA microinjections

• Toxicity and Drug Screening

• Phenotypic Analysis

- Visualization, Documentation and Interpretation

• Consulting and Training

Manipulation of Gene Expression in Zebrafish

RNA

Morpholino(MO)

(knockdown)

cDNA

(transient& mosaic)

cDNA (stabletransgenic)

Phenotype analyses in zebrafish

• Morphology and/or histology

•whole-mount in situ hybridization histochemistry (ISHH)

• whole-mount immunohistochemistry (IHC)

• fluorescent marker (EGFP) expression (living embryos)

• acridine orange apoptosis assay (living embryos)

• cell transplantation and lineage tracing (living embryos)

Toxicity and drug screening in zebrafish

96 or 384 well format

wt Ezrin MO1 wt NCS305787

wt NSC668394 wt NSC668394

Novel compound activity assay

Small compound library screening

Transgene based assays

Shared Resources are partially supported by the Georgetown Howard University Center for Clinical and Translational Studies (GHUCCTS) 1UL1RR031975-01

In your publications, remember to mention support provided from the GHUCCTS.