Post on 25-Feb-2016
description
Dr. William Shaw Lima, Peru 14-15 de mayo, 2005
Why use The Great Plains Laboratory?
The Great Plains Laboratory has performed more than 100,000 tests of persons on the autistic spectrum throughout the world.
Free consultation with testing by phone/Skype 2 autistic children of Great Plains employees have
completely recovered using suggestions of Great Plains
Licensed by the USA government Performs external proficiency testing for all
substances for which it does testing, including many of which are voluntary
Great customer service by highly experienced staff.
William Shaw PhD
How to obtain test kits • Contact The Great Plains Laboratory by
phone, fax, or email• Phone 1 913 341-8949• Fax 1 913 341-6207• Email: gpl4u@aol.com• Videos on how to prepare the test samples on
the website at www.greatplainslaboratory.com
William Shaw PhD
Test kits• Contain all necessary containers to transport samples• Contains information to ship by FEDEX• Contains all necessary information to ship samples• Contains payment information, MasterCard, Visa, or electronic bank transfer
William Shaw PhD
ORGANIC ACID TEST-Great Plains72 Compounds and ratios
- Yeast/Fungal Metabolites-no
- Bacterial Metabolites-no
- B-vitamin Deficiencies-no
- Antioxidant Deficiencies-no
- Inborn Errors of Metabolism
- High Oxalate Levels-no
-
- Neurotransmitter metabolism-no- Indications of diabetic conditions- Citric acid (Krebs) cycle metabolites- Fatty acid abnormalities- Clostridia overgrowth-no- Glycolysis metabolites- Amino Acid metabolites- Pyrimidines-no-72 compounds total
William Shaw PhD
Specific autism markers present on The Great Plains Laboratory Organic Acid Test
-
William Shaw PhD
Normal intestine
=bad bacteria
Intestine
Y = yeast* = yeast product
Blood vessel
Rest of body
Kidney
Urinecup
Y * B
Y
*
*
*
*
*
BBBB
BB BB B
BBB
B B
BBBB BBB B
B
BB BB
B = good bacteria
William Shaw PhD
Y Y YY
YY
*YYY
* *********
***
**
****
*******
*******
**
************** ********
***
*****
** * * ****
** B*
**
*
***
***
* B**
***
*****
*****
********
****
****
****
***********
**********
** ** ** * *
* *
After antibiotics
=bad bacteria
Intestine
Y = yeast* = yeast product
Blood vessel
Rest of body
Kidney
Urinecup
B = good bacteria
William Shaw PhD
Nutritional Neuroscience 2010 Vol 13 No 3: 1-10
William Shaw PhD
HPHPA
Shaw, W Increased Urinary Excretion of Analogs of Krebs Cycle Metabolites and Arabinose in Two Brothers with Autistic Features.
Clin Chem 41:1094-1104, 1995
William Shaw PhD
• C. difficile-pseudomenbranous colitis
• C. sporogenes• C. botulinum-food
poisoning
• C. mangenoti• C. ghoni• C. bifermentans• C. caloritolerans
Clostridia species that produce HPHPA precursors Arch Microbiol 1976; 107:283–288
William Shaw PhD
Two months of nystatin and Lactobacillus acidophilus GG therapy in a child with autism
Candida krusei stool
Yeast tartaric urine*
Lacto-bacillus stool
Clostridia HPHPA urine*
Before
4+
993
0
3265
After
0
1
4+
174
normal range
0-1+
0-15
3+ - 4+
0-150 mmol/mol creatinine
William Shaw PhD
CH2CHCOOH
NH2
CH2CHCOOH
NH2
HO
tyrosine
CH2CHCOOH
NH2
HO
DOPA HO
Dopamine Norepinephrine
phenylalanine
phenylalanine hydroxylase
tyrosine hydroxylase
CH2CH2NH2 HO
HO Dopamine beta-hydroxylase
DOPA decarboxylase
CHCH2NH2 HO
HO
OH
X
CH2CH2NH2
HO
HO
Clostridia dopamine analog
HVA
VMA
3-hydroxyphenylalanine
Clostridia DOPA analog
William Shaw PhD
William Shaw PhD
HVA/VMA= 5:1
DopamineNorepi, Epi
Altered dopamine/norepinephrine ratio with Clostridia excess
Dopamine/ Norepi, Epi
William Shaw PhD
HVA/VMA= 2.58 (Excess dopamine)
Before treatment (depression)
William Shaw PhD
After treatment
HVA/VMA= 1.38
William Shaw PhD
J Child Neurol 2000 Jul;15(7):429-35 Short-term benefit from oral vancomycin treatment of regressive-onset autism. Sandler RH, Finegold SM, Bolte ER, Buchanan CP, Maxwell AP, Vaisanen ML, Nelson,MN, Wexler HM
•11 children with regressive-onset autism were recruited for an intervention trial using vancomycin. •A clinical psychologist blinded to treatment status..noted
improvement in 8 of 10 children studied. •“…these results indicate that a possible gut flora-brain connection warrants further investigation”
William Shaw PhD
William Shaw PhD
William Shaw PhD
William Shaw PhD
William Shaw PhD
Staghorn oxalate crystal in kidney
William Shaw PhD
0
100
200
300
400
500
600
700
oxal
ate
mm
ol/m
ol c
reat
inin
e
AutisticSpectrumN=100
NormalChildrenN=16
p <10-16
t-test
Comparison of urine oxalate in autistic spectrum and normal children
William Shaw PhD
• Children exposed to pesticides called organophosphates used to kill insects had more than twice the risk of developing pervasive developmental disorder (PDD)
• For organochlorines, there was 7X autism rate• Mothers exposed to such pesticides were also likely to
have shorter pregnancies and their children to have impaired reflexes
Maternal Residence Near Agricultural Pesticide Applications and Autism Spectrum Disorders among
Children in the California Central Valley Eric M. Roberts, et al. Environ Health Perspect.
115(10): 1482–1489, 2007
William Shaw PhD
I. Hertz-Picciotto, et al Public Health Sciences and the M.I.N.D. Institute, University of California at Davis, International Meeting for Autism Research (IMFAR) Household Pesticide Use in Relation to Autism, May 2008
• Mothers exposed to pet shampoos (almost all pyrethrins) were twice as likely to have a child with autism.
• The worst exposure time was the second trimester.
William Shaw PhD
William Shaw PhD
William Shaw PhD
William Shaw PhD
R Cade et al. Autism and schizophrenia: Intestinal disorders. Nutritional Neuroscience 3: 57-72,2000 Depts. of Medicine, Physiology, Psychology, and Psychiatry, University of Florida, USA• Abnormal peptides in both patients with autism and
schizophrenia• High titers of IgG antibodies to gliadin (wheat) found
in 87% of patients with autism and 86% of patients with schizophrenia
• High titers of IgG antibodies to bovine casein found in 90% of patients with autism and 93% of patients with schizophrenia
• A gluten and casein free diet caused significant improvement in 81% of patients with autism within 3 months
William Shaw PhD
• Efficacy of a cow's milk free diet (or other foods which gave a positive result after a skin test) in 36 autistic patients.
• Marked improvement in the behavioral symptoms of patients after a period of 8 weeks on an elimination diet
• High levels of IgA antigen specific antibodies for casein, lactalbumin and beta-lactoglobulin
• High IgG and IgM for casein
Lucarelli S, Panminerva Med 1995 Sep;37(3):137-41 Food allergy and infantile autism.
William Shaw PhD
0
50
100
150
200
250
IgG
cas
ein
mg/
L
normal autism
Great Plains data
William Shaw PhD
IgG Comprehensive Food Allergy Test - Autism
William Shaw PhD
• Blood draw • Fingerstick done home
• Blood draw only
IgG antibody test
IgE antibody test
William Shaw PhD
William Shaw PhD
William Shaw PhD
Great Plains Immune Deficiency Profile
William Shaw PhD
Great Plains Immune Deficiency Profile
William Shaw PhD
Great Plains Immune Deficiency Profile
William Shaw PhD
Immune deficiencies in autism• IgA deficiency• Total IgG deficiency• IgG subclass deficiency• Complement C4b deficiency• Myeloperoxidase deficiency
• Severe combined immunodeficiency disorder (SCID)• Normal antibody concentrations but lack of specific antibodies, even though multiple vaccines given
William Shaw PhD
Abnormally elevated IgE in child with autism and severe self abuse
Test child Reference rangeIgG 845 639-1349 mg/dLIgA 157 70-312 mg/dLIgM 81 56-352 mg/dLIgE 1295 10-180 IU/ml
Repeat IgE 1730 10-180 IU/ml
William Shaw PhD
Autism and seizures Immunoglobulin Child’s value Reference range IgG 576 mg/dL 672-1680 mg/dL IgA 67 mg/dL 71-263 mg/dL IgM 28 mg/dL 47-209 mg/dL IgE 2 IU/ml 2-35 IU/ml IgG-1 408 mg/dL 456-952 mg/dL IgG-2 77 mg/dL 147-493 mg/dL IgG-3 85 mg/dL 12-179 mg/dL IgG-4 6 mg/dL 0-168 mg/dL secretory IgA 1 10-20 mcg/ml
William Shaw PhD
Recurrence of Candida: The most difficult medical problem in
autism• Because of depression of cellular immunity,
the person with autism becomes re-infected immediately after antifungal treatment stops
• Some parents report deterioration if even a single dose of antifungal drug is missed
• Some children on antifungals for over 10 years
William Shaw PhD
Streptococcus antibodies and autism-contribute to stereotypical behavior
William Shaw PhD
Streptococcus antibodies and autismfollowup
• Throat rapid strep test• Throat culture• Cultures may give false negative results• Group A beta-hemolytic streptococcus• Antibodies may cause behavioral alteration even if strep organism is missing• Prophylactic antibiotics • Plasmapharesis and replacement• Selective removal of plasma anti-strep antibodies• IVIG-itravenous gamma globulin
William Shaw PhD
Hair metals in boys with autismn=40 boys per group, age matched
0
2
4
6
8
Uranium Mercury Lead
MedianAutismMcg/ghair
MedianNormalMcg/ghair
Autism 9:290-298,2005
William Shaw PhD
Chelation Therapy Options
• “To Bind” from Greek word chele, or claw.• Oral DMSA or TD-DMSA TD=transdermal• Oral DMPS and/or TD-DMPS • Oral EDTA and/or TD-EDTA• Intravenous – DMPS, EDTA• Suppository – Detoxamin (good for lead,
aluminum)• Natural Remedies – Chlorella, Cilantro,
Homeopathic, etc.• Sublingual vs Baths.• Combination of different forms.
William Shaw PhD
Recommended testing summary• Hair screen for long term exposure to toxic elements• If all toxic elements are negative with hair metals, consider
urine challenge test with DMSA,DMPS• Evaluate copper/zinc balance with copper/zinc profile from
Great Plains• Use urine test (without challenge) to evaluate calcium levels• Fecal metals can indicate oral exposure to metals especially
lead but does not identify source• Use X-ray fluorescence to determine sources of lead• Whole blood or RBC good for acute toxicity
William Shaw PhD
Recommended treatment• Find and eliminate sources of toxicity• Remove amalgams• Control Candida since DMSA and DMPS stimulate Candida
overgrowth• Oral DMSA and DMPS cheap, safe, and effective but
stimulate Candida and can make heavy metals worse if oral ingestion is still occurring.
• Extended treatment needed to remove toxic metals from brain
• Do periodic liver tests and blood counts• Supplement with beneficial metals (Chelate-Mate®)• Discontinue if tests abnormal or rare severe symptoms
William Shaw PhD
Safety and Efficacy of Oral DMSA Therapy for Children with Autism SpectrumDisorders: Part B - Behavioral ResultsBMC Clinical Pharmacology 2009, 9:17 doi:10.1186/1472-6904-9-17James B Adams (jim.adams@asu.edu)Matthew Baral (m.baral@scnm.edu)Elizabeth Geis (autismstudynurseasu@gmail.com)Jessica Mitchell (j.mitchell@scnm.edu)Julie Ingram (julieaingram@yahoo.com)Andrea Hensley (ahensley11@cox.net)Irene Zappia (izappia@aol.com)Sanford Newmark (snewmark@email.arizona.edu)Eva Gehn (egehn@asu.edu)Robert A Rubin (brubin698@earthlink.net)Ken Mitchell (kbmpd@yahoo.com)Jeff Bradstreet (drbradstreet@aol.com)Jane El-Dahr (jeldahr@tulane.edu)
William Shaw PhD
Cholesterol in children with autistic spectrum disorder
0
1
2
3
4
5
6
7
8
9
0-20 21-40
41-60
61-80
81-100
101-120
121-140
141-160
161-180
181-200
201-220
221-240
241-260
261-280
281-300
301-320
321-340
341-360
Range of Cholesterol Results-mg/dL
Num
ber o
f pa
tient
s in
gro
up
Low cholesterol 57.5%
Extremely low cholesterol 17.5%
The Great Plains Laboratory cholesterol studyOf children with autistic spectrum disorder
William Shaw PhD
Abnormalities of cholesterol metabolism in autism spectrum disorders. Am J of Med Genetics Part B: Neuropsychiatric Genetics Vol 141B, Issue 6, Pages 666 – 668,2006. Elaine Tierney et al.
● Cholesterol was measured in 100 samples from subjects with ASD using GC/MS
●19 samples (19%) had total cholesterol levels lower than 100 mg/dl, which is below the 5th centile for children over age 2 years.
● These findings suggest that, in addition to SLOS, there may be other disorders of cholesterol metabolism associated with ASD.
William Shaw PhD
Reports of cholesterol supplements in autism-Case 3
• I wanted to let you know that my son’s cholesterol went up 20 points to 131 after taking Sonic® for a month. He is improving wonderfully. However, the proof is in way more than the numbers. Babbling is back and he is watching my lips when I talk to him.
William Shaw PhD
Reports of cholesterol supplements in autism-Case 3-continued
• He understands and follows verbal commands. He learned to give himself a drink from a cup (I used to give him a bottle) during the first month on sonic, two weeks ago he learned to climb stairs and yesterday he fed himself with a spoon. Best of all, he is happy now! He smiles and laughs -- with people!!! At 12 months of age my son would be best described as catatonic, rather than autistic -- he was extremely severe.• .
William Shaw PhD
www.nbnus.com
Toll free 877 575-2467
William Shaw PhD
The plasma zinc/serum copper ratio as a biomarker in children with autism spectrum disorders. Scott Faber et al Biomarkers March 11,2009
• A retrospective review of plasma zinc, serum copper and zinc/copper was performed on data from 230 children with autistic disorder, pervasive developmental disorder-NOS and Asperger's syndrome.
• The entire cohort's mean zinc level was 77.2 mcg/dl; mean copper level was 131.5 mcg/dl, and mean Zn/Cu was .608, which was below the 0.7 cut-off of the lowest 2.5% of healthy children.
• The plasma zinc/serum copper ratio may be a biomarker of heavy metal, particularly mercury, toxicity in children with ASDs.
William Shaw PhD
Comprehensive BiochemicalTesting-Great Plains
• Organic acid test for genetic disease, toxics, and yeast and bacterial byproducts
• Yeast culture and sensitivity
• Cholesterol profile• Heavy metals
hair,blood
• Copper/zinc profile• Comprehensive IgG food
allergies• Comprehensive inhalant
allergies• Immune deficiency• Amino acids and fatty
acids• Streptococcus antibodies
William Shaw PhD
Thank you!