TOXICOLOGY USING DAPHNIA, VIBRIO AND

NITRATE SOLUTIONVictoria McLain

Briana PriceAustin GravesChris ForcinitoGideon Whaley

Josh TaylorSam Holden

Can Daphnia Magna and Vibrio Fischeri be used in a quick and simple nitrogen test?

MATERIALS (Daphnia) 4 100 ml graduated

cylinders 4 storage bottles 4 flasks Sodium Nitrate (s) Sharpie Centigram Balance Weigh boats Tape

PROCEDURE Fill graduated cylinders to 100 ml of

dH2O Weigh 10 g of Sodium Nitrate Add the 100 ml of dH2O into flask Add 10 g of Sodium Nitrate to flask

Mix well Put in storage container and label

concentration Repeat procedure with varying

weights of Sodium Nitrate depending on wanted concentration

Percent solution= (dry mass in grams/ volume in mL) *100

Solution Number (x)

Day of Death for Daphnia (y)

100 parts/million 12

1000 parts/million 6

10000 parts/million 4

100000 parts/million 2

100 pa

rts/m

illion

1000 p

arts/m

illion

10000

parts

/millio

n

10000

0 part

s/milio

n0

2

4

6

8

10

12

14

Day of Death for Daphnia (y)

Day of Death for Daphnia (y)

Parts per million (y)

Number of Days to death

of sample population (x)

CONCLUSION Over the course of this experiment, we

did indeed test the Daphnia. However, we do not believe that the data that we collected is exclusive enough to answer the question that was posed. We only tested a few sets of Daphnia. We were not able to test them in increments

of minutes, but days. The Daphnia took a substantially long amount

of time to produce evidence, or change.

Materials (Vibrio) Slants containing bacteria. Test tubes containing broth. Heat source. Incubator. Plastic rods. Petri Dishes. Small paper discs.

Vibrio FischeriWe have concluded that, as per our original

research question about the efficiency of using Vibrio Fischeri for nitrogen testing, Vibrio Fischeri is not an expeditious tester.

We found the following issues: Growth time Obscure effects Saltwater vs. Freshwater Fragile

WORKS CITED http://upload.wikimedia.org/wikipedia/co

mmons/c/c0/Daphnia_magna.png http://slowmuse.files.wordpress.com/200

9/02/daphnia-15551.jpg https://wildco.com/images/pctr/4120-A10.

jpg http://dl.clackamas.cc.or.us/ch104-01b/im

ages/104vol01.jpg