Post on 27-May-2020
Thermo Scientific Orion VERSA STAR™ pH/ISE/Temperature Module
Reference Guide
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Thermo Scientific Orion VERSA STAR™ pH/ISE/Temperature Module
Table of Contents
Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Chapter 2 Module Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Module Connections and Inputs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3Module Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4Display Information From Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Chapter 3 Setup Menus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Setup Menus for Primary Measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5Read Type Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6Isopotential Value . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7Linear Regression Information . . . . . . . . . . . . . . . . . . . . . . . .7Low Level Stability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7
Setup Menu for Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Chapter 4 pH, RmV and ORP Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
pH Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9RmV Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9ORP Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Chapter 5 ISE Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Chapter 6 Temperature Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Chapter 7 Measurement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
pH/mV/RmV/ORP Measurement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15ISE Measurement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Chapter 8 Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Chapter 9 Incremental Techniques . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Known Addition and Subtraction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19Single Known Addition . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20Double Known Addition . . . . . . . . . . . . . . . . . . . . . . . . . . . .20Single Known Subtraction . . . . . . . . . . . . . . . . . . . . . . . . . .21Double Known Subtraction . . . . . . . . . . . . . . . . . . . . . . . . .21
Analyte Addition and Subtraction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22To Access Incremental Techniques . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Chapter 10 Data Storage and Review . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Data Storage Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23Measurement Read Type . . . . . . . . . . . . . . . . . . . . . . . . . . .23Datalog, Computer and Printer Export Settings . . . . . . . . . .23
Datalog Review . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23Calibration Review . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Chapter 11 Customer Services . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Troubleshooting Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25Assistance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25Warranty and Registration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25WEEE Compliance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25Declaration of Conformity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26pH/ISE/Temperature Module Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Appendix Advanced Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Automatic pH Buffer Recognition Feature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33Testing a pH Electrode for Automatic Buffer Recognition . 33
pH Electrode Condition Icon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34Multipoint Calibration Curve Feature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35Automatic Blank (Non-Linear) Correction Feature . . . . . . . . . . . . . . . . . . . . . . . 36Determining an Isopotential Value . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
1Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Chapter 1 Introduction
Thank you for your purchase of the Thermo Scientific Orion VERSA STAR™ pH/ISE/Temperature Module.
This module is for use with the Thermo Scientific Orion VERSA STAR Advanced Electrochemistry Benchtop Meter.
Some key features of this module include:
• pHrangefrom-2to20withresolutionoptionsof0.001,0.01and0.1
• ISErangefrom0to19999withresolutionofuptofoursignificantdigits
• mVandRmVrangesfrom±2000mV
• Automaticandmanualdataloggingoptions
• Upto6-pointpHcalibrationwithautomaticrecognitionforUSA/NISTandDINbuffers
• Upto6-pointISEcalibrationwithadjustableISOpointcapabilities
• TheabilitytofixpH/ISEcalibrationerrorswithoutacompleterecalibrationthankstocalibrationediting
• AdvancedISEfeaturessuchasauto-blank,timedendpoint,linearpoint-to-point,non-linearselectable
auto-blank,low-concentrationrangestability,ISEIDandincrementaltechniques
Pleasereadthisreferenceguidethoroughly.Anyuseoutsideoftheseinstructionsmayinvalidateyourwarrantyand
causepermanentdamagetothemeter.
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3Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Chapter 2 Module Overview
Toconnectthemodulestothemeterandforspecificinformationregardingthemeteroutputs,pleaseseethe
OrionVERSASTARReferenceGuide.TheReferenceGuideisontheCDincludedwiththemeterandalsoavailable
at www.thermoscientific.com/water.
Module Connections and Inputs
TheOrionVERSASTARmetercanacceptOrioncombinationISEelectrodesaswellasseparatereferenceandsensing
half-cellelectrodes.ThisallowsforawiderISEmeasurementoffering.Half-cellelectrodesseparatetheionssensing
fromthereferenceelectrode.Fordifficultsamples,theflexibilityofusingalternatefillsolutionsmaybeanadvantage
to researchers.
1. PreparethepHorISEelectrodeandATCprobeforuseasinstructedintheelectrodeuserguides.
The pin tip reference connector is on the lowest panel of the pH/ISE module.
2. PlacethepHorISE,ATCprobeandOrionStarstirrerprobe(cat.no.096019,optional)intothe
electrode arm.
3. AttachthepHorISEBNCandATCprobetothepH/ISEmetermodule.LowerISEandATCinto
solutionforconditioningifrequired.Ifusingthestirrerprobe,attachprobetothemeter,placeintothe
electrodearmandassigntheprobetothechannelinthesetupmenuforinstrumentsettings.
4. Preparefreshstandardsdailyforcalibration.ForISEtesting,standardsshouldbeatleastonedecade
(10times)apart.Thestandardsshouldexceedtheexpectedminimumandmaximumsamplerange.
Serialdilutionisrecommendedusingcalibratedpipettestoaccuratelypreparecalibrationstandards
from a stock solution.
5. Gatherreagents,rinsewater,andothersupplies.
ForISEtesting,ifISAwillbeaddedtothesample,itneedstobeaddedtoallthestandardspriorto
calibrationtoensureaconsistentdilutionfactor.
pH/ISE Module
8pinmini-DINforATC
BNCconnectionwithshortingcap
Reference pin socket
4 Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Module Maintenance
Forroutinemaintenance,dustandwipethemodulewithadampcloth.Ifnecessary,warmwaterormildwater-based
detergentcanbeused.Maintenancecanbeperformedonadaily,weeklyormonthlybasis,asrequiredbyoperating
environment.Immediatelyremoveanyspilledsubstanceusingthepropercleaningprocedureforthatspilltype.
Display Information from Module
ExampleISEMeasurementScreen–SingleChannel
Dateandtime
Primaryreading
Calibrationinformation
CorrespondingmV value
Method
Theinformationonthedisplaycanbecustomized.Enterthesetupmenutomakechanges.1.PressSetup.2.UsethearrowkeystohighlightInst.Settings, press (f3/select) key.3.UsethearrowkeytohighlightDisplayView. Press (f3/select) key.4. Follow the onscreen prompts to choose what you would like to see on the measurement screen.
5Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Chapter 3 Setup Menus
Setup Menus for Primary Measurements
1. PressSetup (f3) to access the Main Setup Screen.
2. UsethearrowkeytoscrollacrosstothemodulestoISEchannelyouwishtouseand
press Select (f3).
3. Scroll to across to the mode menu and press Select (f3).
ISE Setup Menu (Default values are in bold in the table below)ElectrodeSerialNumber InputElectrode Type SeetablebelowSampleID Off,Manual,AutoIncrementSignificantDigits 1,2,3,4digitsStability Smart,Fast,Medium,SlowAveraging Off,Automatic Smart Read Type AutoRead,Timed,ContinuousMeasureUnit ppm,Molar,mg/L,%,ppb,NoneIsopotential Value Off,OnLinearRegression Off,OnBlank Correction Off,OnLowLevelStability Off,OnAlarm** Limit,CalDue,SetPoint
**Limitandsetpointalarmdefaultsetto‘off’. Calduealarmsetto12hours.
pH Setup Menu (Default values are in bold in the table below)
ElectrodeID InputSampleID Off,Manual,AutoIncrementResolution 0.1,0.01,0 .001Stability Smart,Fast,Medium,SlowAveraging Off,Automatic SmartRead Type AutoRead,Timed,ContinuousBuffer Group USA,DIN,CustomIsopotential Value Off,On 7 .000LinearRegression Off,OnAlarm** Limit,CalDue,SetPoint
**Limitandsetpointalarmdefaultsetto‘off’. Calduealarmsetto12hours.
6 Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
4. ForISEtesting,selecttheISEelectrodebeingusedinsetup.Ifyourelectrodeisnotlisted,
usethe“X”electrodewiththevalence(+1,+2,-1,or-2)matchingyourelectrodefor
accurate results.
Read Type Information
Therearethreemainreadtypesavailableasshowninthesetupmenus.
• Auto-Read – Themeterwilldisplaythemeasurementasitstabilizesandlockandholdthe
measurementwhenitisstable(TheARiconwilllockonthescreen.)Oncethemeasurementislocked,
themeterwillautomaticallyexportthemeasurementtothedatalog,ifthedatalogisenabledin
thesetupmenu,andtoaprinterorcomputer,ifaprinterorcomputerisconnectedtothemeterand
enabledinthesetupmenu.Totakeanewmeasurement,pressmeasure (esc)/1.
• Timed – Themeterwillreadanddisplayameasurementatthesettimeintervalthatisprogrammed
bytheoperator.Therearetwooptions:
1. Bychecking/selectingthe"Interval"box,atthesettimeinterval,themeterwillautomatically
exportthemeasurementtothedatalog,ifthedatalogisenabledinthesetupmenu,andto
aprinterorcomputer,ifaprinterorcomputerisconnectedtothemeterandenabledinthe
setupmenu.Ifselected,enterthetimeintervalvalueinahours:minutes:secondsformatusing
the numeric keypad and press f3 (select) key.
2. Ifthe"Interval"boxisunchecked/notselected,asingletimedmeasurementwillbetaken.
Themeterwillapplythesamesavingandexportingconditionsaswiththesettimeintervalto
recordandexportdata,butlockafteronetimedintervalhasexpired.Pressmeasure (esc)/1 to
resetthecountdownclockandtakeanewreading.
ISE TypesAg+ I- X- CO2 REDOX(ORP) Cl2 S-2 ClO4 O2
Cd+2 NH3 BF-4 K+ X-2 Cu+2 X+ F- SCN-CN- Pb+2 Cl- NO3- Br- NOx Ca+2 Na+ X+2
ORP, RmV and mV Setup Menus (Default values are in bold in the table below)
ElectrodeID InputSampleID Off,Manual,AutoIncrementResolution 1.0,0 .1Stability Smart,Fast,Medium,SlowAveraging Off,Automatic SmartRead Type AutoRead,Timed,ContinuousAlarm** Limit,CalDue*,SetPoint
*Only for ORP and RmV modes. **Limitandsetpointalarmdefaultsetto‘off’. Calduealarmsetto12hours.
7Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
• Continuous – The meter will continuously measure and update the display. This read type is useful
whenperforminganexperimentthatrequirescontinuousmeasurementstobetaken.Pressthe
log/print/0printkeytoexportthemeasurementtothedatalog,ifthedatalogisenabledinthesetup
menu,andtoaprinterorcomputer,ifaprinterorcomputerisconnectedtothemeterandenabledin
the setup.
Isopotential Value
Theslopeofallelectrodeschangeswithtemperature,basedontheNerstequation.Theslopecurvesatthedifferent
temperaturesintersectattheisopotentialvalue,whichistheconcentrationatwhichthepotentialoftheelectrodedoes
notvarywithtemperature.ForglasspHelectrodes,thisvalueis7.00.Forotherionselectiveelectrodes(ISEs),ifthe
isopotentialvalueisknownorcanbemeasuredexperimentally,adjustingthisvaluemakestemperaturecompensation
foranISEpossible.Temperaturecompensationcanbeperformedforoneandtwopointcalibrations.
Linear Regression Information
Thedefaultisoffwhichusesthetraditional,segmentedapproach.Thesegmentedapproachisthemostaccurateand
precise.MoreinformationcanbefoundintheappendixsectionofthisreferenceguideundertheMultipointCalibration
Curve Feature.
Selecting“on”forthisoptionresultsintheuseofasinglebestfitlineforthecalibrationcurve,andcanbebeneficialif
pHmeasurementsvarygreatly.
Low Level Stability
ThisfeatureadjuststhetimingforthecalibrationpointsforlowerlevelISEstandards.
Setup Menu for Temperature
AllofthetemperaturemeasurementsettingsarefoundinTemperatureSetupmenuforeachmeasurement.
*Thisnumberwillchangetomatchthesetupmenuchannel.
Temperatureprobescanbesetupinthemeasurementmodeofthesampleyouaremeasuring.Ch-1ATCallowsthe
temperatureprobetobeusedinmorethanonechannelfortemperature-compensatedmeasurements,suchaswhen
apHelectrodeanda2-cellconductivitycellareinthesamemeasurementsample.TemperatureisacriticalpHand
conductivitymeasurementasthemeasurementvaluesrequireatemperaturecorrectiontodeterminethereference
value,often25ºCintheAmericaand20ºCinEurope.
Temperature Unit ºCorºFselectionTemperature Input and Type Ch-1ATC,Ch-2ATC,Ch-3ATC,Ch-4ATC,MTC
Temperature Cal Ch-1*ATConlyorAllATCallowsallATCstobe calibratedatonce
8 Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
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9Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Chapter 4 pH, RmV and ORP Calibration
pH Calibration
UptosixpHbufferscanbeusedforcalibration.Thiscanbeacombinationofautomatically-recognizedUSA/NISTor
DINbuffervaluesandcustombuffers.AlwaysusefreshpHbuffersandselectbuffersthatbracketthesamplepHand
areatleastonepHunitapart.PreparethepHelectrodeaccordingtotheinstructionsintheelectrodeuserguide.
1. Pressf1 (Cal)toperformapHcalibration.
2. Followscreenpromptsforcalibration.Usefreshbuffers,rinseelectrodewithDIwaterbeforeeach
reading,blotwithalintfreetissue.
3. USAandDINbuffersetsareautomaticallyrecognizedbasedofftheselectioninthesetupmenu,
andthemeterallowscustombufferstobeusedforupto6calibrationpoints.Custombufferoption
prompts for the nominal values and stores data.
4. Duringcalibration,pointscanbeeditedorretestedpriortoacceptance.
5. Informationforthelastcalibrationcanbedisplayedonthemainmeasurementscreen.Refertothis
referenceguide,Chapter2ModuleOverview,DisplayInformationsectionformoreinformation.
RmV Calibration
OnestandardcanbeusedforrelativemVcalibration.Alwaysusefreshstandard.Preparetheelectrodeaccordingto
theinstructionsintheelectrodeuserguide.
1. Pressf1 (Cal) toentercalibrationmode.
2. Followthescreenpromptsforcalibration.
3. Thecalibrationcanbesavedorprintedwhencomplete.
4. Informationforthelastcalibrationcanbedisplayedonthemainmeasurementscreen.Refertothis
referenceguide,Chapter2ModuleOverview,DisplayInformationsectionformoreinformation.
10 Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
ORP Calibration
ThemVreadingofanORPstandardisdependentontheORPelectrodereferencesystem,referencematerial,filling
solutionandORPstandardtemperature.AnORPelectrodecanbeautomaticallycalibratedtoread420mVrelativeto
theStandardHydrogenElectrode(EH)at25°CwhentheOrionORPstandard(cat.no.967901or96791),OrionORP
electrode(cat.no.9678BNWP,9778BNWP,9180BNMDor9179BNMD)andOrion4MKClfillingsolution(cat.no.
900011)or4MKClgel(inelectrodewithcat.no.9179BNMD)areused.TherawmVreadingofanelectrodewiththe
sameconditionsis220mVat25°C.
OnestandardcanbeusedforORPcalibration.Alwaysusefreshstandard.Preparetheelectrodeaccordingtothe
instructionsintheelectrodeuserguide.
1. Pressf1 (Cal)toentercalibrationmode.
2. Followthescreenpromptsforcalibration.
3. Thecalibrationcanbesavedorprintedwhencomplete.
4. Informationforthelastcalibrationcanbedisplayedonthemainmeasurementscreen.Refertothis
referenceguide,Chapter2ModuleOverview,DisplayInformationsectionformoreinformation.
11Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Chapter 5 ISE Calibration
OnetosixstandardscanbeusedforISEcalibration.Ifmorethanonestandardisused,startwiththelowest
concentrationfirst.Alwaysusefreshstandards.Selectstandardsthatbracketthesampleconcentrationandarea
decadeapart.Preparetheionselectiveelectrodeaccordingtotheelectrodeuseguide.
1. Presschannel key until ISE channel is displayed. Press Cal (f1).Followthescreenpromptsstarting
with the lowest concentration.
2. PressEdit (f3)tothecorrectconcentrationvalueofastandardifitisnotautomaticallyrecognized.
3. Afterthefirstdatapointhasbeenaccepted,pressAccept (2) tocontinuecalibration.
The meter then offers options of Esc (f1)toleavecalibration,Next (f2)measurenextcalibration
point,orCal Done (f3).
4. TheoptionsafterCalDoneispressedarePrint (f1),Cal Edit (f2) or Slope Edit (f3).
5. TheCalibrationreporttableisdisplayedwiththeconcentration,mVreading,andTemperatureand
averageslopewhichisupdatedwitheachsuccessivepointmeasured.
6. Fromthisscreenthenavigationoptionsare Print (f1),Cal Edit (f2) or Slope Edit (f3).Printexports
datatoprinterorcomputer,Caleditallowsconcentrationtobecorrectedpriortothecompletionof
thecalibrationbyDelete (f1) or ReMeasure (f3).
7. PressingtheSlopeEditchangesthescreentitletoSinglePointCal–SlopeEdit.Thisallowsthe
currentslopelinetobeupdatedwiththepresentelectrodeperformance.
8. Press Cal Done (f1)whencompleted,pressviewgraph,savethecalibrationinCalLog.
12 Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
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13Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Chapter 6 Temperature Calibration
TheATCtemperaturedisplayhasarelativeaccuracyof±0.1°C.ATCprobes(bothseparateandthosebuiltinto
electrodes)havevaryingtemperatureaccuracies,usually±0.5°Cto±2°C.Use this function only if necessary.
Sincethetemperatureoffsetcalculatedduringthecalibrationisappliedtoallfuturetemperaturemeasurements,
recalibrateifadifferentATCprobeisused.Fortemperaturecalibration,theprobeneedstobeconnectedtothemeter
andthecalibrationsolutionshouldhaveaknown,stabletemperature.ItisrecommendedthattwoNIST-traceable
thermometersbeusedtomeasureandverifysolutiontemperature.
1. Pressf3 (setup) key.
2. Usethearrowkeystohighlightthechannelthatthetemperatureprobeisconnectedto.
Press f3 (select).
3. UsethearrowkeystohighlightTemperatureandpressf3 (select).
4. UsethearrowkeystohighlighttheappropriateTemperatureCaloptionandpressf3 (select).
5. Follow the onscreen prompts.
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15Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Chapter 7 Measurement
pH/mV/RmV/ORP Measurement
Afterasuccessfulcalibration,thesamplescanbetested.
1. Rinsetheelectrodewithdeionizedwater.Blotdrywithlint-freetissue.
2. Placeelectrode/ATCprobeinfirstsampleandstirgently.
3. IfthemeterisinAutoReadmode(meterdefault)pressmeasure (esc) (1) key. If the meter is in
continuousortimedreadmode,themeterwillimmediatelystarttakingreadings.
4. IfinAutoReadmodeanddatalogisenabled(default),thereadingwillautomatically
bestoredwhenthe“AR”appears.Ifincontinuousreadmodeanddatalogisenabled,
press log/print (0) keytostoreintothemeter’smemory.
Note: Toenableordisabledatalogging,presssetupandselectInst.Settings.
ISE Measurement
1. Preparethesampleasinstructedintheelectrodeuserguide.
2. FollowdirectionscarefullyonISAandothersampleadditions.
3. Rinsetheprobe(s)andplaceintothesample.IfthemeterisinAutoReadmode(meterdefault)press
measure (esc) (1)key.Ifthemeterisincontinuousortimedreadmode,
themeterwillimmediatelystarttakingreadings.
4. IfinAutoReadmodeanddatalogisenabled(default),thereadingwillautomaticallybestoredwhen
the“AR”appears.Ifincontinuousreadmodeanddatalogisenabled,presslog/print (0) key to store
intothemeter’smemory.
Note:Toenableordisabledatalogging,presssetupandselectInst.Settings.
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17Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Chapter 8 Methods
Themeterstoresuptotenmethodsperchannelwiththelastcalibrationdatastoredforeachmethod.
Usethechannelsetuptoaccessandmodifymethods.
1. Pressf3 (setup) key.
2. Usethearrowkeystohighlighttheappropriatechannel.Pressf3 (select).
3. UsethearrowkeystohighlightMethodandpressf3 (select).
4. Themethodscanbeloaded,copied,savedandeditedbyfollowingtheonscreenprompts.
18 Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
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19Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Chapter 9 Incremental Techniques
Known Addition and Subtraction
TheOrionVERSASTARmeterhaspresetmethodsforsingleknownaddition,singleknownsubtraction,doubleknown
additionanddoubleknownsubtraction.
• Whenperformingincrementaltechniques,thesampleconcentrationmustbeknownwithinan
orderofmagnitudesotheincrementmaybecorrectlychosenandthesampleandstandardmustbe
volumetrically determined.
• Itisveryimportantthatanycomplexingagentispresentinexcess(atleast50to100times)ornot
atall.Theratiooffreetocomplexedionsmustremainconstantovertheaddition.Anindicationthat
acomplexingagentispresent,butnotingreatenoughquantitytomaintainaconstantfreeionto
complexedionratio,isanabnormallyhighorlowslope.Ifthisisasuspectedproblem,addanexcess
amountofcomplexingordecomplexingagenttotheoriginalsolutioninthebeaker.
• Electrodeinterferencesshouldbeataminimum,sincetheeffectoftheinterferencemightchange
astheconcentrationoftheionofinterestchanges.Thissituationmayalsoresultinhighorlow
electrodeslopes.Consulttheelectrodeuserguideforinformationonreducingoreliminatingsome
electrode interferences.
• Knownadditionisausefulmethodformeasuringdilutesamples,occasionalsamplesorsamplesthat
containanexcessofcomplexingagent.Knownadditioncanalsobeusedtoverifytheresultsofa
direct measurement.
• Knownsubtractionisusefulwhenmeasuringionsforwhichstablestandardsdonotexist.Known
subtractioncanalsobeusedasaquickversionofatitration.Whenperformingaknownsubtraction,
itisnecessarytoknowthestoichiometricratiobetweenthestandardandsample.
• Doubleincrementaltechniquescalculatetheelectrodeslopeandsampleconcentration
simultaneously.Theelectrodeslopeisdetermineddirectlyinthesample,whichresultsingreater
accuracyforsampleswithcomplexmatricesandgreateranalysisspeed.
20 Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Single Known Addition
A standard of known concentration is added to a sample of unknown concentration. The total concentration should
approximatelydoubleupontheadditionofthestandard,thereforesampleconcentrationshouldbeknownwithinan
orderofmagnitude.Refertothefollowingtabletochoosethecorrectstandardconcentrationandvolumeofaddition.
• Refertotheappropriateelectrodeuserguideforinstructionsforpreparingtheelectrode,required
solutions(ionicstrengthadjusters,standards,etc)andanyspecialrequirements.Determinethe
electrodeslopeasdirectedintheelectrodeuserguide.
• Ensurethattheelectrode,ATCprobeandreferenceelectrode(ifapplicable)areconnectedtothe
meter inputs that correspond with the selected channel.
Double Known Addition
Formostdoubleknownadditionanalysis,itisrecommendedthat:
• Thesamplevolumeshouldbe100mL.
• Theconcentrationofthestandardaddedshouldbe100timestheexpectedsampleconcentration.
• Calculatethevolumeofstandardrequiredtohalvetheinitialsampleconcentrationanddivideby
six.Fivesixthsofthevolumewillbeaddedinthefirstadditionandtheremainderinthesecond.If
available,consultaprintoutfromapreviousanalysisofasimilarsample.Note:theproposedvaluesfor
constantmVwillbesmallerthanthoserecommendedforknownaddition.Thisisanefforttominimize
mechanceofover"subtracting"thesample(overshootingtheendpoint).
Refertotheappropriateelectrodeuserguideforinstructionsforpreparingtheelectrode,requiredsolutions
(ionicstrengthadjusters,standards,etc)andanyspecialrequirements.
Ensurethattheelectrode,ATCprobeandreferenceelectrode(ifapplicable)areconnectedtothemeterinputsthat
correspond with the selected channel.
Amount of standard to be added per 100 mL of sample
Standard concentration compared to expected sample concentration
1mL 100timesmoreconcentrated5mL 20timesmoreconcentrated10mL 10timesmoreconcentrated
21Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Single Known Subtraction
Inknownsubtraction,eachadditionofstandardsubtractsanincrementofthespeciestobemeasured.Enterthe
correctreactionratioofthespeciestobemeasuredwiththestandardtobeadded.Forexample,asulfide(S-2)sample
istobesubtractedbytheadditionofsilver(Ag+).Twosilverionsarerequiredtosubtracteachsulfideionandthe
reactionratiois0.5.
Sampleconcentrationshouldbeknownwithinanorderofmagnitudeandthetotalsampleconcentrationshould
beapproximatelyhalveduponadditionofthestandard.Refertothefollowingtabletochoosethecorrectstandard
concentration and volume of addition.
Refertotheappropriateelectrodeuserguideforinstructionsforpreparingtheelectrode,requiredsolutions(ionic
strengthadjusters,standards,etc)andanyspecialrequirements.Determinetheelectrodeslopeasdirectedinthe
electrodeuserguide.
Ensurethattheelectrode,ATCprobeandreferenceelectrode(ifapplicable)areconnectedtothemeterinputsthat
correspond with the selected channel.
Double Known Subtraction
Ensurethatthesubtractionabilityofthechosenstandardconcentrationandvolumeaddeddoesnotexceedthe
concentration of sample.
Formostdoubleknownsubtractionanalysis,itisrecommendedthat:
• Thesamplevolumeshouldbe100mL.
• Theconcentrationofthestandardaddedshouldbe50timestheexpectedsampleconcentration.
• Calculatethevolumeofstandardrequiredtohalvetheinitialsampleconcentrationanddivideby
six.Fivesixthsofthevolumewillbeaddedinthefirstadditionandtheremainderinthesecond.If
available,consultaprintoutfromapreviousanalysisofasimilarsample.Note:theproposedvaluesfor
constantmVwillbesmallerthanthoserecommendedforknownaddition.Thisisanefforttominimize
mechanceofover"subtracting"thesample(overshootingtheendpoint).
Refertotheappropriateelectrodeuserguideforinstructionsforpreparingtheelectrode,requiredsolutions(ionic
strengthadjusters,standards,etc)andanyspecialrequirements.
Ensurethattheelectrode,ATCprobeandreferenceelectrode(ifapplicable)areconnectedtothemeterinputsthat
correspond with the selected channel.
Amount of standard to be added per 100 mL of sample
Standard concentration compared to expected sample concentration
1mL 50timesmoreconcentrated5mL 10timesmoreconcentrated10mL 5 times more concentrated
22 Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Analyte Addition and Subtraction
InAnalyteAddition,thefirstmeasurementisinaknownstandardsolutioncontainingthesametypeofanalytein
interest as the sample. The second measurement follows the addition of the unknown solution. The concentration of
the unknown is then calculated.
InAnalyteSubtraction,thefirstmeasurementusesknownstandardsolutioncontainingthetypeofionsthattheISE
canmeasureandthatwillcomplexwiththeanalyteininterestintheunknownsample.Thesecondmeasurement
follows the addition of the unknown sample that reduces the concentration of the ions in the measurement solution.
The concentration of the analyte in interest in the unknown is then calculated.
• Isopotentialvalueneedstobeknownandentered.
• Blankcorrectionisnotapplied.
• Theslopevalueat25°Cneedstobeknownandentered.
To Access Incremental Techniques
1. SetthemeasurementmodetoISE.
2. Pressf3 (setup) key.
3. UsethearrowkeystohighlighttheISEchannel.Pressf3 (select).
4. UsethearrowkeystohighlightIncrementalTechniquesandpressf3 (select).
5. Follow the onscreen prompts.
23Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Chapter 10 Data Storage and Review
Data Storage Settings
Measurement Read Type
The measurement read type determines when the meter sends measurements to outputs as selected in the setup menu
and when properly connected to the meter. These are set individually for each channel. Measurement Read Type is set
perchannelinthesetupmenu.Pleasereferchapter3SetupMenusinthisreferenceguideformoreinformation.
Datalog, Computer and Printer Export Settings
OrionVERSASTARMetershavea2000-datalogcapability.Tomakeyourselection:
1. Frommeasurementmode,presssetup/3.
2. PressthearrowkeystohighlightInst.Settings.Presssetup/3.
3. Usethearrowkeys,f2 (page) key and f3 (select) key to:
a. set the communication protocol
b.turnonprinting
c. select the print format
d.turnondatalogging
Formoreinformationonthesesettings,refertotheOrionVERSASTARReferenceGuide,Chapter3InstrumentSettings
toturnonDataLogtoenabledatastorage.
Datalog Review
Thisoptionallowsforstoreddatatobe:
• reviewed
• printedindividually,inagrouporall
• deleted
• analyzedwithgeneralstatisticalinformation
Thedatalogwillreflectascreencaptureofalltheinformationatthetimeofdatalogging.
Toreviewdataloginformation
• Presslog view (9) key
• Pressf3 (select) key
• Followpromptstoreviewinformation
24 Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Calibration Review
Thisfeatureallowsforreviewandprintingofeachofthelast10calibrationsforeachparameterofthemeter.
Theinformationwillmatchtheinformationrecordedforthatcalibration.
Toreviewcalibrationinformation
1. Presslog view (9) key.
2. Pressright arrow (6) keytoselectCalLoginformationanddown arrow (8)tohighlightthe
desired parameter.
3. Press f3 (select) key and follow the prompts.
25Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Chapter 11 Customer Services
Troubleshooting Guide
Assistance
Aftertroubleshootingallcomponentsofyourmeasurementsystem,contactTechnicalSupport.WithintheUnitedStatescall1.800.225.1480andoutsidetheUnitedStatescall978.232.6000orfax978.232.6031.InEurope,theMiddleEastandAfrica,contactyourlocalauthorizeddealer.Forthemostcurrentcontactinformation,orthelatestapplicationandtechnicalresourcesforThermoScientificOrionproducts,visitwww.thermoscientific.com/water.
Warranty and Registration
Toregisteryournewmeterandforthemostcurrentwarrantyinformation,visitwww.thermoscientific.com/water.
WEEE Compliance
ThisproductisrequiredtocomplywiththeEuropeanUnion’sWasteElectrical&ElectronicEquipment(WEEE)Directive2002/96/EC.Itismarkedwiththesymbolabove. ThermoFisherScientifichascontractedwithoneormorerecycling/disposalcompaniesineachEUMemberStateandthisproductshouldbedisposedoforrecycledthroughthem.Furtherinformationoncompliancewiththesedirectives,therecyclersinyourcountry,andinformationonThermoScientificOrionproductsthatmayassistthedetectionofsubstancessubjecttotheRoHSDirectiveareavailableatwww.thermoscientific.com.
Error Recommended Actions
Measurement is flashing9999andoverrangeorunderrange.
Measurementisoutsidetheallowablemeasurementrange.Checkelectrodeconnectionandsettingsinthesetupmenu.Cleantheelectrodeaccordingtotheelectrodeuserguideandrecalibratetheelectrodewithnewbuffersorstandards. Iftheerrorcontinues,performthemeterselftestinthediagnosticsmenu.
The measurement freezesandwill notchange.
ThemeterisinAutoReadmode.(ARiconappearssolidonthedisplay.)Press measure (esc)/1keytotakeanewreadingorgotosetuptochangethereadtype.
26 Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Declaration of Conformity
Manufacturer: Thermo Fisher Scientific Inc .
Address: AyerRajahCrescent Blk55#04-16/24 Singapore139949 Singapore
Hereby declares that the following products:Orion VERSA STAR Advanced Electrochemistry Benchtop Meters with Modules are rated 100to240VAC,50/60Hz,0.5A.
Equipment Class:
Measurement,controlandlaboratoryOrionStarA-seriesmetersareEMCClassA
Conforms with the following directives and standards:
EN61326-1:2006 Electromagnetic Compatibility (EMC Directive) Electricalequipmentformeasurement, controlandlaboratoryuse-EMCrequirements
EN61010-1:2001 Safety StandardsUL61010-1:2004 Safetyrequirementsforelectricalequipmentformeasurement,CAN/CSA C22 .2 No . 61010-1-04 controlandlaboratoryuse-generalrequirements
CheowKwangChan QA/RegulatoryManager
PlaceandDateofIssue: 12December,2011 Singapore
27Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
pH/ISE Module (VSTAR-ISE) SpecificationspH Range -2.000to20.000
Resolution 0.1,0.01,0.001Relative Accuracy ±0.002CalibrationPoints Upto6CalibrationEditing YesAdjustableISOPoint Yes
mV/RmV Range ±2000.0mVResolution 0.1Relative Accuracy ±0.2mVor±0.05%ofreadingwhicheverisgreaterEH ORP Mode Yes
ISE Range 0to19999Resolution Upto4significantdigitsRelative Accuracy ±0.2mVor±0.05%ofreadingwhicheverisgreaterUnits ppm,M,mg/L,%,ppb,noneCalibrationPoints Upto6CalibrationEditing YesAdjustableISOPoint Yes
CalibrationFeatures Timedendpoint,linearpointtopoint,non-linearselectableauto-blank,lowconcentrationrangestability
IncrementalTechniquesSingleknownaddition&subtraction, doubleknownaddition&subtraction, singleanalyteaddition&subtraction
Advanced Features Displayselectrodetype,temperaturecompensationTemperature Range -5to105°C,23to221ºF
Resolution 0.1Relative Accuracy ±0.1OffsetCalibration 1point
Inputs pH/ISE:BNC,referencepin ATC:8-pinmini-DIN
pH/ISE/Temperature Module Specifications
NOTE:Wereservetherighttomakeimprovements.Specificationssubjecttochangewithoutnotice.
28 Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Ordering Information
CML # DescriptionVSTAR00 Orion VERSA STAR Benchtop Meter
-Electrodearmwithredesignedholder -Universalpoweradapter
VSTAR10 Orion VERSA STAR pH Benchtop Meter Set -OrionVERSASTARmeterwithpH/temperaturemodule -Electrodearmwithredesignedholder -Universalpoweradapter
VSTAR12 Orion VERSA STAR pH Benchtop Meter Kit -OrionVERSASTARmeterwithpH/temperaturemodule -8302BNUMDOrionROSSTriodepH/ATCprobe -096019OrionStarstirrerprobe -810199ROSSsolutionkit(475mLeachofpH4,7and10buffers; storagesolution;cleaningsolution;andpHelectrodestoragebottle) -Electrodearmwithredesignedholder -Universalpoweradapter
VSTAR20 Orion VERSA STAR Conductivity Benchtop Meter Set -OrionVERSASTARmeter -OrionVERSASTARconductivity/temperaturemodule -Electrodearmwithredesignedholder -Universalpoweradapter
VSTAR22 Orion VERSA STAR Conductivity Benchtop Meter Kit -OrionVERSASTARmeter -OrionVERSASTARconductivity/temperaturemodule -013005MDOrionDuraProbeconductivitycell,K=0.475 -011007conductivitystandard,1413µS,5x60mL -Electrodearmwithredesignedholder -Universalpoweradapter
VSTAR30 Orion VERSA STAR RDO/Dissolved Oxygen Benchtop Meter Set -OrionVERSASTARmeter -OrionVERSASTARdissolvedoxygen/temperaturemodule -Electrodearmwithredesignedholder -Universalpoweradapter
VSTAR32 Orion VERSA STAR RDO/Dissolved Oxygen Benchtop Meter Kit -OrionVERSASTARmeter -OrionVERSASTARdissolvedoxygen/temperaturemodule -083005MDOrionpolarographicDOprobe -080017calibrationsleeveforDOprobe -080513DOprobemaintenancekit -970802BODfunnel/stirrer -080360BODadapter -Electrodearmwithredesignedholder -Universalpoweradapter
29Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
CML # DescriptionVSTAR40A Orion VERSA STAR pH/ISE Benchtop Meter Set
-OrionVERSASTARmeter -OrionVERSASTARpH/ISE/temperaturemodule -Electrodearmwithredesignedholder -Universalpoweradapter
VSTAR40A2 Orion VERSA STAR pH/ISE Benchtop Meter Kit -OrionVERSASTARmeter -OrionVERSASTARpH/ISE/temperaturemodule -8102BNUWPOrionROSSUltrapHelectrode -096019OrionStarstirrerprobe -927007MDATCprobe,stainlesssteel -ROSSsolutionkit(475mLeachofpH4,7and10buffers;storagesolution; cleaningsolution;andpHelectrodestoragebottle) -Electrodearmwithredesignedholder -Universalpoweradapter
VSTAR40B Orion VERSA STAR Benchtop Meter with Two pH/ISE Modules -OrionVERSASTARmeter -TwoOrionVERSASTARpH/ISE/temperaturemodules -Electrodearmwithredesignedholder -Universalpoweradapter
VSTAR40B2 Orion VERSA STAR Benchtop Meter with Two pH/ISE Modules Kit -OrionVERSASTARmeter -TwoOrionVERSASTARpH/ISE/temperaturemodules -8102BNUWPOrionROSSUltrapHelectrode -096019OrionStarstirrerprobe -927007MDATCprobe,stainlesssteel -ROSSsolutionkit(475mLeachofpH4,7and10buffers;storagesolution; cleaningsolution;andpHelectrodestoragebottle) -Twoelectrodearmswithredesignedholders -Universalpoweradapter
VSTAR50 Orion VERSA STAR pH/Conductivity Benchtop Meter Set -OrionVERSASTARmeter -OrionVERSASTARpH/temperaturemodule -OrionVERSASTARconductivity/temperaturemodule -Electrodearmwithredesignedholder -Universalpoweradapter
VSTAR52 Orion VERSA STAR pH/Conductivity Benchtop Meter Kit -OrionVERSASTARmeterwithpH/temperaturemodule -OrionVERSASTARconductivity/temperaturemodule -8157BNUMDOrionROSSUltraTriodepH/ATCprobe -013005MDOrionDuraProbeconductivitycell,K=0.475 -810199ROSSsolutionkit(475mLeachofpH4,7and10buffers; storagesolution;cleaningsolution;andpHelectrodestoragebottle) -011007conductivitystandard,1413µS,5x60mL -Electrodearmwithredesignedholder -Universalpoweradapter
30 Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
CML # DescriptionVSTAR80 Orion VERSA STAR pH with LogR Benchtop Meter Set
-OrionVERSASTARmeter -OrionVERSASTARpH/temperaturewithLogRtechnologymodule -Electrodearmwithredesignedholder -Universalpoweradapter
VSTAR82 Orion VERSA STAR pH Benchtop Meter Kit -OrionVERSASTARmeter -OrionVERSASTARpH/temperaturewithLogRtechnologymodule -8172BNXOrionROSSSure-FlowpHelectrode -927007MDATCprobe,stainlesssteel -ROSSsolutionkit(475mLeachofpH4,7and10buffers;storagesolution; cleaningsolution;andpHelectrodestoragebottle) -810007ROSSfillsolution,5x60mL -Electrodearmwithredesignedholder -Universalpoweradapter
VSTAR90 Orion VERSA STAR pH/ISE/Conductivity/RDO/Dissolved Oxygen Benchtop Multiparameter Meter Set -OrionVERSASTARmeter -OrionVERSASTARpH/ISE/temperaturemodule -OrionVERSASTARconductivity/temperaturemodule -OrionVERSASTARdissolvedoxygen/temperaturemodule -Electrodearmwithredesignedholder -Universalpoweradapter
VSTAR91 Orion VERSA STAR pH/ISE/Conductivity/RDO/Dissolved Oxygen Benchtop Multiparameter Meter Set -OrionVERSASTARmeter -TwoOrionVERSASTARpH/ISE/temperaturemodules -OrionVERSASTARconductivity/temperaturemodule -OrionVERSASTARdissolvedoxygen/temperaturemodule -Two096019OrionStarstirrerprobes -Twoelectrodearmswithredesignedholders -Universalpoweradapter
31Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
CML # DescriptionVSTAR92 Orion VERSA STAR pH/ISE/Conductivity/RDO/Dissolved Oxygen
Benchtop Multiparameter Meter Set -OrionVERSASTARmeter -OrionVERSASTARpH/ISE/temperaturemodule -OrionVERSASTARconductivity/temperaturemodule -OrionVERSASTARdissolvedoxygen/temperaturemodule -8157BNUMDOrionROSSUltraTriodepH/ATCprobe -013005MDOrionDuraProbeconductivitycell,K=0.475 -083005MDOrionpolarographicDOprobe -080017calibrationsleeveforDOprobe -080513DOprobemaintenancekit -970802BODfunnel/stirrer -080360BODadapter -810199ROSSsolutionkit(475mLeachofpH4,7and10buffers; storagesolution;cleaningsolution;andpHelectrodestoragebottle) -011007conductivitystandard,1413µS,5x60mL -Electrodearmwithredesignedholder -Universalpoweradapter
VSTAR93 Orion VERSA STAR pH/ISE/Conductivity/RDO/Dissolved Oxygen Ultimate Benchtop Multiparameter Meter Set -OrionVERSASTARmeter -OrionVERSASTARpH/ISE/temperaturemodule -OrionVERSASTARconductivity/temperaturemodule -OrionVERSASTARdissolvedoxygen/temperaturemodule -8157BNUMDOrionROSSUltraTriodepH/ATCprobe -013005MDOrionDuraProbeconductivitycell,K=0.475 -083005MDOrionpolarographicDOprobe -Two096019OrionStarstirrerprobes -080017calibrationsleeveforDOprobe -080513DOprobemaintenancekit -970802BODfunnel/stirrer -080360BODadapter -810199ROSSsolutionkit(475mLeachofpH4,7and10buffers; storagesolution;cleaningsolution;andpHelectrodestoragebottle) -011007conductivitystandard,1413µS,5x60mL -Twoelectrodearmswithredesignedholders -Universalpoweradapter
32 Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
AccessoriesCML # DescriptionVSTAR-PH Orion VERSA STAR pH/temperature moduleVSTAR-ISE Orion VERSA STAR pH/ISE/temperature moduleVSTAR-CND Orion VERSA STAR conductivity/temperature moduleVSTAR-RD OrionVERSASTARRDO/DO/temperaturemoduleVSTAR-LR OrionVERSASTARpH/temperaturewithLogRtechnologymoduleSTARA-BEA BenchtopelectrodearmforOrionStarA-seriesmetersSTARA-HB FreestandingbaseforusewithOrionStarA-seriesbenchtopelectrodearmSTARA-PWR Replacement universal power adapter for Orion VERSA STAR meters096019 OrionStarstirrerprobe8102BNUWP OrionROSSUltrapHelectrode,refillable,glassbody8172BNWP OrionROSSSure-FlowpHelectrode,glassbody8156BNUWP OrionROSSUltrarefillablepHelectrode,epoxybody8157BNUMD OrionROSSTriodepH/ATCelectrode,refillable,epoxybody8302BNUMD OrionROSSTriodepH/ATCelectrode,refillable,glassbody8172BNX OrionROSSSure-FlowpHelectrode,glassbody8611BNWP OrionROSSsodiumcombinationISE927007MD OrionATCprobe,stainlesssteelbody927005MD OrionATCprobe,epoxybody013005MD OrionDuraProbeconductivitycell,4-cell,K=0.475013016MD Orionconductivitycell,flowthrough,K=0.1083005MD Oriondissolvedoxygenprobe,polarographic086030MD Orionauto-stirDO/BODprobe,polarographic087010MD OrionRDOopticalDOprobe,3mcable810199 OrionROSSall-in-onepHbufferkit910104 OrionpH4.01buffer,475mL910107 OrionpH7.00buffer,475mL910110 OrionpH4.01buffer,475mL011008 Orionconductivitystandard,100µS/cm,5x60mLbottles011007 Orionconductivitystandard,1413µS/cm,5x60mLbottles011006 Orionconductivitystandard,12.9mS/cm,5x60mLbottles1010001 Orionconductivitycalibrationresistorkit9512HPBNWP Orionhigh-performanceammoniaISE9512BNWP Orion standard ammonia ISE951007 Ammoniastandard,1000ppm,475mL951210 Low-levelammoniaISA951211 AmmoniaISA,475mL951209 FillsolutionforOrionhigh-performanceammoniaISE,60mL951202 FillsolutionforstandardammoniaISE,60mL951213 AmmoniaISEstoragesolution9609BNWP OrionfluoridecombinationISE040906 Fluoridestandard,1ppmwithTISABII,475mL040907 Fluoridestandard,2ppmwithTISABII,475mL040908 Fluoridestandard,10ppmwithTISABII,475mL940909 TISABII,1gallon8611BNWP OrionROSSsodiumcombinationISE
33Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Appendix Advanced Features
Automatic pH Buffer Recognition Feature
TheOrionVERSASTARMeterwithpH/ISE/TemperaturemodulesystemsarecapableofautomaticallyrecognizingpH1.68,4.01,7.00,10.01and12.46buffersorpH1.68,4.01,6.86,and9.18buffersduringapHcalibration,dependingonthepHbuffersetthatisselectedinthesetupmenu.Duringacalibration,themeterusestheselectedbuffersetandtherawmVreadingofthepHelectrodeinthebuffertorecognizeanddisplaythebuffervalueatthemeasuredtemperature.TherawmVreadingofthepHelectrodeinthebuffermustbeabout±30mVfromthetheoreticalmVreadingofthebufferinorderforthemetertoautomaticallyrecognizethebuffer.
USA pH Buffer Set DIN pH Buffer SetBuffer mV Range Buffer mV Range
1.68 +285to+345mV 1.68 +285to+345mV4.01 +207to+147mV 4.01 +207to+147mV7.00 -30to+30mV 6.86 +38to-22mV10.01 -207to-147mV 9.18 -99to-159mV12.46 -293to-353mV
Testing a pH Electrode for Automatic Buffer Recognition
ToverifythattherawmVreadingofthepHelectrodeinuseis±30mVfromthetheoreticalmVreadingofthebuffer,andthereforeverifythatthepHelectrodeinuseiscapableofperformingautomaticbufferrecognition,performthefollowingprocedure.
1. SetthemeasurementmodetomV.PreparethepHelectrodeaccordingtotheinstructionsinthepHelectrodeuserguide.
2. RinsethepHelectrodewithdistilledwater,blotitdrywithalint-freetissueandplacethepHelectrodeintoapH4.01bufferatapproximately25°C.
3. WaitforthemeasurementtostabilizeandrecordthemVvalueofthepH4.01bufferwhenthemeterindicatesthatthemeasurementisstable.ThereadtypeselectedinthesetupmenuwilldeterminehowthemVmeasurementsaredisplayedbythemeter.
4. RemovethepHelectrodefromthepH4.01buffer.
5. RinsethepHelectrodewithdistilledwater,blotitdrywithalint-freetissueandplacethepHelectrodeintoapH7.00bufferatapproximately25°C.
6. WaitforthemeasurementtostabilizeandrecordthemVvalueofthepH7.00bufferwhenthemeterindicatesthatthemeasurementisstable.
7. ThemVreadingofthepHelectrodeinpH4.01buffershouldbeintherangeof+207to+147mVandthemVreadingofthepHelectrodeinpH7.00buffershouldbeintherangeof-30to+30mV.IfthemVreadingsofthepHelectrodeareinthecorrectranges,thepHelectrodeiscapableofperformingautomaticbufferrecognition.IfthemVreadingsofthepHelectrodearenotinthecorrectranges,thevaluesofthepHbufferswillneedtobemanuallyenteredduringapHcalibration.
34 Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
pH Electrode Condition Icon
TheelectrodeconditioniconindicatestheperformanceofthepHelectrode,basedonthelastsavedcalibration andelectrodemeasurementstability.
Icon pH Electrode Definition of Icon
Electrodeconditionisgoodandtheelectrodeslopeis90to110%.
Electrodeconditionisfairandtheelectrodeslopeis80to120%.
Electrodeconditionisbadandtheelectrodeslopeislessthan80%orgreater than120%.
Consulttheelectrodeuserguideforinstructionsonhowtoclean,conditionandtroubleshootthe electrode.
35Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Multipoint Calibration Curve Feature
TheOrionVERSASTARMeterwithpH/ISE/Temperaturemodulesystemsutilizeapoint-to-pointschemeformultipointcalibrationsinthepHandISEmodes.Inthistechnique,straightlinesegmentsaredrawnbetweeneachsuccessivepairofcalibrationpoints.SeparateslopeandE0valuesarecalculatedusingtheadjacentpairofdatapoints.Forexample,inafivepointcalibration,fourslopesandfiveE0valuesarecalculatedtocorrespondtothefourlinesegmentsthatconnectthedata.pHorconcentrationvaluesforsamplesarecalculatedaccordingtothesegmentthattheirpotentialvaluesfallinto.Thoseaboveorbelowtherangeofcalibrationarecalculatedbyextrapolationfromthetoporbottommostsegment.
Thedisplayedslopeisanaverageslopeoftheseparatesegments.Theslopemaybeoutsidetherangenormallyexpectedforanelectrode.Forexample,iftheslopeofonesegmentislow,thentheaveragewillbelowerthantheoretical,eventhoughtheresponsemaybeNernstianovermostoftherange.
Thefigurebelowisanexampleofatypicalcalibrationcurveusingthismethod.
36 Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Automatic Blank (Non-linear) Correction Feature
TheOrionVERSASTARMeterwithpH/ISE/Temperaturemodulesystemshaveafeatureknownasautomaticblankcorrection.Inthistechnique,themeterdecideswhetherblankcorrectionisthebestmeasurementstrategybyanalyzingtheelectroderesponseduringamultipointcalibration.Aseparateblankdoesnothavetoberun.Graphically,automaticblankcorrectionisequivalenttodrawingasmoothcurvethroughthelowestthreepointsofthemultipointcalibrationandextrapolatingtozeroconcentrationbasedontheassumptionofNernstianelectrodebehavior.
Multipointcalibrationsatthelowerlimitofdetectionaredesirablewhentheresponseofanionselectiveelectrodeisnon-linearandcannotbecharacterizedwithaoneortwopointcalibration.Thisisusuallyseenasalowelectrodeslope.Generally,theelectrodeisbehavinginaNernstianmannerbuttheeffectofablankisbeingobserved.Seethefigurebelow.
Thisblankmaybeatruereagentblank,tracesofanalyteioninthereagentsoritmaybethe“mud”valueoftheelectrode.Itcouldalsobeaninterferenceinthereagentsthatbecomesapparentatlowlevelsofanalyteion,oritcouldbeanycombinationoftheseeffects.TheexpandedversionoftheNernstequationtraditionallyusedforblankcorrectionis as follows:
E = Eo + S * log (C + b) wherebistheblank
Inamulti-pointcalibration,asetofequationsisgeneratedandtherelationshipbetweenthemevaluated.Forexampletheequationsgeneratedinathreepointcalibrationwouldbeasfollows:
E1 = Eo + S * log (C1 + b)
E2 = Eo + S x log (C2 + b)
E3 = Eo + S x log (C3 + b)
ThemeterevaluatestherelationshipsbetweenthethreepotentialsE1,E2andE3andthethreeconcentrationsC1,C2 andC3.Iftherelationshipsindicatethatblankcorrectionisdesired,ablankwillautomaticallybecalculatedandthe non-linearitywillbecorrectedforinaNernstianmanner.Iftheappropriateconditionsarenotmet,theblankissetatzeroandeachsegmentofthemultipointcalibrationistreatedindependently.
37Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Whenallthreeofthefollowingconditionsaremet,blankcorrectionisinvoked.
1. Theconcentrationofthefirststandardiszero,ortheslopeoftheelectrodebetweenthefirstandsecondstandardsislessthantheslopebetweenthesecondandthird.
2. Potentialdifferencesbetweenpointsaresignificant.Forexample,E3-E1>10mV
3. Theblankcorrectionalgorithmconvergesatreasonableblankandslopevalues.Conditionsinsteps1and2preventfailuretoconvergeinmostsituations.However,theslopewillattainanyvaluenecessaryforconvergencetoacalculatedblankvalueof3xC3.
Whenblankcorrectionisimplemented,theslopevaluefortheelectrodemaybeoutsidetherangeofvaluesnormallyconsideredacceptableduringanordinarycalibration.Forbestresults,calibrationstandardsshouldbecloseinrangetotheexpectedsampleconcentrationsandshouldbrackettheexpectedsampleconcentration.Whenconditions1,2and3arenotmet,thecalibrationdataishandledbythemultipointcalibrationmethoddescribedearlier.
Incalibrationswithmorethanthreepoints,acombinationofmethodsisutilized.Automaticblankcorrectionisusedifthelowestthreepointssatisfythecriteriaandmultipointcalibrationisusedfortheotherpoints.Theslopedisplayedaftercalibrationisanaverageoftheslopecalculatedintheautomaticblankcorrectionalgorithmandtheslope(s)foreachadditionalsegment.
Determining an Isopotential Value
1. Prepareseveralstandardswithconcentrationrangesoverthemeasuringrangeoftheelectrode.
2. Measurethemillivoltvalueofthestandardsatroomtemperature,about20°Cto25°C.
3. Measurethemillivoltvalueofeachstandardat75°C.
4. Measurethemillivoltvalueofeachstandardat10°C.
5. Onsemi-logarithmicgraphpaper,plottheconcentrationvaluesonthelogaxisversusthemillivoltvaluesonthelinearaxis.
6. Thelineswillintersectattheisopotentialvalue.Readtheconcentrationfromthegraphforthispointfromthelogaxis.Thisistheisopotentialvalue.
38 Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Notes
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