SUPERNOVA A MONOMERIC PHOTOSENSITIZING FLUORESCENT PROTEIN FOR

CHROMOPHORE-ASSISTED LIGHT INACTIVATION

KIWAMU TAKEMOTO ET AL. 2013SCIENTIFIC REPORTS

PHOTOSENSITIZERS

Bulina et al., 2006

CALI CHROMOPHORE-ASSISTED LIGHT INACTIVATION

Photosensitizer ProteinTarget Protein

intense light irradiation Target inactivation

Cellular Death

PHOTOSENSITIZERS

Li et al., 2012

KILLER RED VS SUPER NOVA

Takemoto et al., 2013, Bulina et al., 2006

579 nm610 nm

SuperNova KillerRed EGFP

Cx43

Keratin

Fibrillarin

KILLER RED VS SUPER NOVA

Scale bar 10µmTakemoto et al., 2013,

EXPERIMENTAL RESULTS

0 15 30

45 60 75 90

Takemoto et al., 2013, Bulina et al., 2006Magnification 100x

EXPERIMENTAL RESULTSActin-GFP Cofilin-SuperNova

BeforeCALI

AfterCALI

Takemoto et al., 2013Scale bar 10µm

CALI APPLICATIONS

Metaphase Early Cytokinesis Late Cytokinesis Death Survival

Ou et al., 2012

CALI IN C.ELEGANS

Scale bar 5µm Scale bar 2,5µm

WT CALI QR.a QR.a (pig-1) QR.p

REFERENCES

Wei Li, Nico Stuurman, Guangshuo Ou. (2012). Chromophore-assisted laser inactivation in neural development. Neuroscience Bulletin. 28 (4), 333–341.

Maria E Bulina, Konstantin A Lukyanov, Olga V Britanova, Daria Onichtchouk, Sergey Lukyanov & Dmitriy M Chudakov. (2006). Chromophore-assisted light inactivation (CALI) using the phototoxic fluorescent protein KillerRed. Nature Protocols. 1 (2), 947-953.

Maria E Bulina, Dmitriy M Chudakov, Olga V Britanova, Yurii G Yanushevich, Dmitry B Staroverov, Tatyana V Chepurnykh, Ekaterina M Merzlyak, Maria A Shkrob Sergey Lukyanov & Konstantin. (2006). A genetically encoded photosensitizer. Nature Biotechnology. 24 (1), 95-99.

Guangshuo Ou, Nico Stuurman, Michael D’Ambrosio, Ronald D. Vale. (2010). Polarized Myosin Produces Unequal-Size Daughters During Asymmetric Cell Division. Science. 330, 677-679.

Takemoto, K., Matsuda, T., Sakai, N., Fu, D., Noda, M., Uchiyama, S., ... & Nagai, T. (2013). SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation. Scientific reports, 3.

Look for diagnosis.com (2014). Chromophore-Assisted Light Inactivation (CALI) using the phototoxic fluorescent protein KillerRed. Available: http://jcs.biologists.org/content/127/8/1621/F1.large.jpg.