1

Transcriptomics with a scalpel, not a hatchet.

Jason Macrander

2

3

GATTACA

A

T

C

G

A TC

G

A TC

G

A

T

C

G

ATC

G

ATC

G

5

GATTACA

A

T

C

G

A TC

G

A TC

G

A

T

C

G

ATC

G

ATC

G

Duplication

Mutation20x1x

GATTACA

A

T

C

G

A TC

G

A TC

G

A

T

C

G

ATC

G

ATC

G

C

C

C

C

G

G

G

G

99

GATTACA

A

T

C

G

ATC

G

ATC

G

A

T

C

G

A TC

G

A TC

G GATTACA

A

T

C

G

ATC

G

ATC

G

A

T

C

G

A TC

G

A TC

G

GATTACA

A

T

C

G

ATC

G

ATC

G

A

T

C

GA

T

C

G

A

T

C

G

GATTACAA

T

C

G

AT

C

G

AT

C

G A

T

C

G

AT

C

G

AT

C

G

1111

12

13

Raw Read Length Transcriptome Size

1414

GATTACA

A

T

C

G

A TC

G

A TC

G

A

T

C

G

ATC

G

ATC

G

14

GATTACA

A

T

C

G

A TC

G

A TC

G

A

T

C

G

ATC

G

ATC

G

15

C G

C

C

C

C

G

G

G

G

C

C

C

C G

G

G

G

16

C

C

C

C G

G

G

G

GATTACAA

T

C

G

A

T

C

G

A

T

C

G

A

T

C

G

A

T

C

G

A

T

C

G

GATTACAA

T

C

G

A

T

C

G

A

T

C

GA

T

C

G

A

T

C

G

A

T

C

G

1717

GATTACA

A

T

C

G

ATC

G

ATC

G

A

T

C

G

A TC

G

A TC

G GATTACA

A

T

C

G

ATC

G

ATC

G

A

T

C

G

A TC

G

A TC

G

GATTACA

A

T

C

G

ATC

G

ATC

G

A

T

C

GA

T

C

G

A

T

C

G

GATTACAA

T

C

G

AT

C

G

AT

C

G A

T

C

G

AT

C

G

AT

C

G

180.2

19