Single Fiber Polymorphism in Skeletal Muscles: A Case Example from the Lobster
Scott Medler
Wolfe, 1985Cell Ultrastructure
Guyton and Hall 1996Textbook of Medical Physiology9th Ed.
Guyton and Hall 1996Textbook of Medical Physiology9th Ed.
Guyton and Hall 1996Textbook of Medical Physiology9th Ed.
S1/S2 MHC expression
Tm expression vs. MHC expression (mRNA)
TnT3 protein vs. S1/S2 MHC mRNA
TnI3 protein vs. S1/S2 MHC mRNA
TnI2 protein vs. S1/S2 MHC mRNA
S2 fibersS1 fibers
S2 MHC
S1 MHC
TnT1
TnT3
CONTINUUM OF ISOFORM EXPRESSION
= high expression = low expression
TnI3
TnI2
S1 Tm
S2 Tm
Immediate Research Focus:
Single fiber polymorphism in lobsters
• Developmental changes in fiber phenotype
• Innervation pattern and fiber phenotype
• Effects of electrical stimulation on fiber phenotype
• Mechanical measurements from single fibers
Long Term Research Interests:
• Single Fiber Polymorphism in Mammals
• Scaling Effects on Cellular/Molecular Organization of Muscles
• Physiology of Avian Flight Muscles
Acknowledgements:
National Institutes of Health: Postdoctoral fellowship AR 08597-01A1 to S. Medler
National Science Foundation: IBN-0077422 to D.L. Mykles
Dr. D.L. Mykles Colorado State University
Travis Lilley Colorado State University