Fluorescence and Biology
Sergi Padilla
George Gabriel Stokes
Pink Floyd?microscope
Gin Tonic and Stokes Experiment
U.V Light!
Quinine found in tonic water!
FRET and Lifetime:
Jelly fish glowing in the dark
Biological light?
Green Fluorescent Protein out of Aquorea Victoria
Fluorescence decay: A population of a bunch of molecules relaxing
∆t
τD
τDA
Fluorescence decay of the donor
FRETefficiency
∆t
τD
donor
Proportionbound donor
log Ilog I
Measurement of the mean lifetime:
•.ACQUISITION BEFORE BLEACHING
•. ACQUISITION AFTER BLEACHING
A Proof of FRET by FLIM
+ +
The Nobel prize in Chemistry 2008