Francisco Fuster

March 18, 2011

SDS of pGLO

SDS is the electrophoresis of proteins. This technique is used to see the quality of the

extracted proteins. Here we see if the proteins are degraded or in good condition. To

determine the condition of the proteins we must look at the bands left on the gel. SDS is

a detergent the basically coats the protein with a negative charge so it can travel down

the gel from the negative origin to the positive destination. This lab is a continuation of

the last lab because we will use the transformed bacteria to see of the with arabinose in

the dish actually produced the protein. Our results indicated that those that didn’t have

arabinose in the medium did not produce the protein because the gene was never

turned on. On the other hand, those with arabinose showed the band that indicates the

presence of GFP. The one with no heat applied actually emitted light, because it wasn’t

denatured. The one that had heat applied was present because it is still the same size,

but it did no emit light because the heat denatured it. The substance that was loaded

into the gel was so viscous that only those that had a wider gel were able to get more

accurate results.