Post on 13-Jan-2016
RF Generator Power 1550W
Argon Gas Flow Rates
Plasma 15 L / Min
Carrier 0.8 L / Min
Makeup 0.35 L / Min
Collision Gas (He) 4 mL / Min
Spray Chamber Temp. 2 °C
Omega Lens 11.0 V
Octopole bias -18 V
Energy Discrimination 4.0 V
Replicates 3
Nebulizer Micromist Concentric
Isotopes monitored 55Mn, 56Fe, 59Co, 63Cu, 66Zn
Supple 1.
0 100 200 300 400 5000
5
10
15
20
25
30
Par
ticl
e num
ber
(%
)
Particle diameter (nm)
Al-R in Water Al-R in GA
Supple 2.
(Weeks)1 2 3 4 5 6 7 8
Bod
y w
eigh
t (g
)
0
525
30
35
40
45
50
Control1.5 mg/kg3 mg/kg6 mg/kg
Supple 3.
0 2 4 6 8 10 12 Necropsy (weeks)
Control 6 mg/kgKidney
Liver Control 6 mg/kg
Supple 4.
0 100 200 300 400 500
0
5
10
15
20
25
30
Par
ticl
e num
ber
(%
)
Particle diameter (nm)
Al-D in Water Al-D in GA Al-R in Water Al-R in GA
Supple 5.
Sample Solution Diameter (nm) Surface charge (mV)
Al-DIn Water 112.2 ± 31.8 53.78 ± 0.73
In GA 137.7 ± 40.2 39.55 ± 0.43
Al-RIn Water 107.5 ± 30.9 72.44 ± 7.25
In GA 92.9 ± 26.3 38.40 ± 1.35
(A) (B)
Supple 6.D
rink
ing
wat
er (
% o
f co
ntro
l, m
l/m
ice
b.w
.)
Die
t (%
of
cont
rol,
g/m
ice
b.w
.)
Al-S Al-R
LD
H in
Kid
ney
(%
of
con
trol
)
0
100
200500
510
520
530
540
Control5 ug/ml20 ug/ml
Al-S Al-R
LD
H in
Liv
er (
% o
f co
ntr
ol)
0
10
2080
100
Control5 ug/ml20 ug/ml
Al-S Al-R
LD
H in
Lu
ng
(% o
f co
ntr
ol)
0
2080
90
100
110
120
130
140
150
160
170
180
Control5 ug/ml20 ug/ml
Al-S Al-R
LD
H in
Hea
rt (
% o
f co
ntr
ol)
0
2080
90
100
110
120
Control5 ug/ml20 ug/ml
**
*
**
**
Supple 7.
Data for review only : Experimental design for consumption of diet and drinking water (n =3)
Cage No.1 Cage No.2 Cage No.3
Diet and drinking water Diet and drinking water Diet and drinking water
Data for review only : Experimental design using the blood
Hematology analysis
Biochemistry (n=4)
Cytokines (n=6)
Blood sampling (about 1.2 ml/mice)
500 μl
The rest: serum after centrifugation
Hematology analysis
500 μl
The rest: serum after centrifugation
200 μl 200 μl
Hematology (n=8)
The rest serum The rest serum
100 μl
Lysis
100 μl 100 μl 100 μl
ICP/MS analysis (n=3)
100 μl 100 μl 100 μl 100 μl 100 μl 100 μl 100 μl 100 μl
Data for review only : Experimental design for ICP/MS